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The map label for this gene is prfC [H]

Identifier: 123965971

GI number: 123965971

Start: 666155

End: 667792

Strand: Direct

Name: prfC [H]

Synonym: P9515_07361

Alternate gene names: 123965971

Gene position: 666155-667792 (Clockwise)

Preceding gene: 123965970

Following gene: 123965972

Centisome position: 39.09

GC content: 34.8

Gene sequence:

>1638_bases
ATGAGTTCAAGTACTAACAACTTAAATAATAAAAATATCTTAGAAGCGGTAAATAAAAGAAGAAACTTTGCGATTATTTC
TCATCCTGATGCGGGTAAAACAACTCTTACTGAAAAACTTCTTTTATATGGAGGAGCAATACAGCAAGCAGGGGCTGTAA
AAGCAAGAGGTAATCAAAGGAAAGCTACTTCTGATTGGATGGACCTTGAAAAACAAAGAGGGATTTCAATTACTTCCACT
GTATTGCAGTTTGAGTATGAAAGAGCAGTAATAAATCTTTTGGATACGCCTGGCCACAAGGATTTCTCAGAAGATACTTA
CAGAACTTTAGCTGCCGCAGATAATGCAGTTATGCTTGAAGATGCGGCTAAAGGATTAGAACCTCAAACAAGAAAATTAT
TTGAGGTATGCAGAATGAGAAAAATACCTATATTTACTTTTATTAACAAGATGGATAGACCAGGAAGAGAACCTTTTTCT
TTGATTGATGAAATTGAGTCGGAACTTGGTTTAAATACTTTGCCAGTAAATTGGCCTATTGGTATGGGGGAGGAATTTAG
AGGAGTTATTGATAGATTTACAAGAGAAGTTATTTTATTTGATAAGGCTGTTAGGGGGAAACAGTCAAATGAGAAAAGAA
TGAGTTTAGATGATAAAGAACTTTCAAAATATGTAGAAAGAGAATTACTTGATATCTCTCTTGAAGAGCTAGAGGTTCTA
GACGAAGCAGGTTCGAAACTAGAAAAAGAAGAAATTTTTAAGGGTTCACTTACGCCTGTTTTTTTTGGTTCGGCGATGAC
TAATTTTGGGGTTAGACCTTTTTTAGATAGTTTTTTAAAAATGGCGCAAAAACCAACTTCAAGGAATAGTAATAAAGGGG
ATATTGAGCCTGCAAGCGATGAATTTAGCGGTTTCGTATTTAAGTTACAAGCAAATATGGATCCAAAACATAGAGATAGG
GTTGCATTTATTAGAGTTTGTAGTGGAAAATTTGAAAAGGATATGTCAGTTAAACATTCAAGGACAGGAAAAACGATTAG
ATTATCTCGACCTCAAAAAATATTTGGTCAAGAAAGAGAAGTTGTTGATGATGCCTATCCTGGGGATGTTATTGGACTAA
ATAACCCAGGGATGTTTTCTATAGGAGATACCTTATATACAGGAACTTTTCTTGAATATGAGGGCATACCCTCCTTTAGT
CCTGAAATATTTTGTTGGTTAAGAAATCCAAATCCTTCAGCATTTAAAAACTTTAGAAAGGGTGTAAACGAACTTCGAGA
AGAAGGTGCTGTTCAAATACTTTATGACTTTGATGAGAGCAAAAGAGATCCTATCCTCGCAGCTGTTGGACAATTACAGT
TGGATGTGGTCATTCATAGATTAAAAAATGAATATGGCGTAGATGCCAATCTTGACTCGATGCCATACCAATTGGCAAGA
TGGGTTTCAGATGGATGGTCAGCTATTGACGAATTAGGTAGAATATTTAATTGTAAAGTAGTAAAAGATTGTTGGAATAG
GCCAGTGATTCTTTTTAAAAATCAGTGGAATCTTAACCAATTTATTGAAGATAACGCTAGTTTTAGTTTAAAAAAAGTAT
CACCTGTTGTTAGTGGCGTGGAACCAATTGCTTTATAA

Upstream 100 bases:

>100_bases
AGAATAAATTATCTTCTTTAGAAGTGTTTGAAGAAGGTGATCAGTATTTAGTCATTTATGGTAAAGTTTAAGGTTAGTGA
GATTGTTAAAATAGACTTTA

Downstream 100 bases:

>100_bases
AGTCCTAATGGATAATAAAATTGGTTAATCTATAAATAATATAAAAAACAATTGGATTCAAATAAAAGCAAAAACAATCC
TGATAAAACACCTTATGAAA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 545; Mature: 544

Protein sequence:

>545_residues
MSSSTNNLNNKNILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMDLEKQRGISITST
VLQFEYERAVINLLDTPGHKDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRKIPIFTFINKMDRPGREPFS
LIDEIESELGLNTLPVNWPIGMGEEFRGVIDRFTREVILFDKAVRGKQSNEKRMSLDDKELSKYVERELLDISLEELEVL
DEAGSKLEKEEIFKGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASDEFSGFVFKLQANMDPKHRDR
VAFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQEREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTFLEYEGIPSFS
PEIFCWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLDVVIHRLKNEYGVDANLDSMPYQLAR
WVSDGWSAIDELGRIFNCKVVKDCWNRPVILFKNQWNLNQFIEDNASFSLKKVSPVVSGVEPIAL

Sequences:

>Translated_545_residues
MSSSTNNLNNKNILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMDLEKQRGISITST
VLQFEYERAVINLLDTPGHKDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRKIPIFTFINKMDRPGREPFS
LIDEIESELGLNTLPVNWPIGMGEEFRGVIDRFTREVILFDKAVRGKQSNEKRMSLDDKELSKYVERELLDISLEELEVL
DEAGSKLEKEEIFKGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASDEFSGFVFKLQANMDPKHRDR
VAFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQEREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTFLEYEGIPSFS
PEIFCWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLDVVIHRLKNEYGVDANLDSMPYQLAR
WVSDGWSAIDELGRIFNCKVVKDCWNRPVILFKNQWNLNQFIEDNASFSLKKVSPVVSGVEPIAL
>Mature_544_residues
SSSTNNLNNKNILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQRKATSDWMDLEKQRGISITSTV
LQFEYERAVINLLDTPGHKDFSEDTYRTLAAADNAVMLEDAAKGLEPQTRKLFEVCRMRKIPIFTFINKMDRPGREPFSL
IDEIESELGLNTLPVNWPIGMGEEFRGVIDRFTREVILFDKAVRGKQSNEKRMSLDDKELSKYVERELLDISLEELEVLD
EAGSKLEKEEIFKGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASDEFSGFVFKLQANMDPKHRDRV
AFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQEREVVDDAYPGDVIGLNNPGMFSIGDTLYTGTFLEYEGIPSFSP
EIFCWLRNPNPSAFKNFRKGVNELREEGAVQILYDFDESKRDPILAAVGQLQLDVVIHRLKNEYGVDANLDSMPYQLARW
VSDGWSAIDELGRIFNCKVVKDCWNRPVILFKNQWNLNQFIEDNASFSLKKVSPVVSGVEPIAL

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=30.9128630705394, Blast_Score=202, Evalue=8e-52,
Organism=Homo sapiens, GI19923640, Length=546, Percent_Identity=26.1904761904762, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI25306287, Length=518, Percent_Identity=26.4478764478764, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=29.0076335877863, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI157426893, Length=150, Percent_Identity=32.6666666666667, Blast_Score=87, Evalue=6e-17,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=33.5766423357664, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI4503483, Length=159, Percent_Identity=30.8176100628931, Blast_Score=76, Evalue=7e-14,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=35.3448275862069, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=35.3448275862069, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=35.3448275862069, Blast_Score=73, Evalue=9e-13,
Organism=Escherichia coli, GI1790835, Length=471, Percent_Identity=49.8938428874735, Blast_Score=473, Evalue=1e-134,
Organism=Escherichia coli, GI1789738, Length=491, Percent_Identity=29.938900203666, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI48994988, Length=149, Percent_Identity=37.5838926174497, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788922, Length=183, Percent_Identity=33.3333333333333, Blast_Score=103, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=27.6859504132231, Blast_Score=182, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI17556745, Length=478, Percent_Identity=27.1966527196653, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557151, Length=152, Percent_Identity=34.2105263157895, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=31.055900621118, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71988819, Length=136, Percent_Identity=32.3529411764706, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=136, Percent_Identity=32.3529411764706, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=480, Percent_Identity=30.4166666666667, Blast_Score=206, Evalue=7e-54,
Organism=Saccharomyces cerevisiae, GI6322359, Length=515, Percent_Identity=26.9902912621359, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6324707, Length=567, Percent_Identity=22.7513227513227, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6320593, Length=567, Percent_Identity=22.7513227513227, Blast_Score=80, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=32.6086956521739, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24582462, Length=489, Percent_Identity=28.6298568507157, Blast_Score=192, Evalue=4e-49,
Organism=Drosophila melanogaster, GI221458488, Length=509, Percent_Identity=26.1296660117878, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI78706572, Length=174, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=32.6797385620915, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28574573, Length=142, Percent_Identity=32.3943661971831, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI281363316, Length=170, Percent_Identity=30.5882352941176, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17864358, Length=170, Percent_Identity=30.5882352941176, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 61746; Mature: 61615

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTNNLNNKNILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQR
CCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCC
KATSDWMDLEKQRGISITSTVLQFEYERAVINLLDTPGHKDFSEDTYRTLAAADNAVMLE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEH
DAAKGLEPQTRKLFEVCRMRKIPIFTFINKMDRPGREPFSLIDEIESELGLNTLPVNWPI
HHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GMGEEFRGVIDRFTREVILFDKAVRGKQSNEKRMSLDDKELSKYVERELLDISLEELEVL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
DEAGSKLEKEEIFKGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASD
HHHHHHCCHHHHHCCCCCHHEECHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFSGFVFKLQANMDPKHRDRVAFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQERE
CCCCEEEEEECCCCCCHHHHEEEEEECCCCCCCCCCCHHCCCCCEEEECCCHHHHCCHHH
VVDDAYPGDVIGLNNPGMFSIGDTLYTGTFLEYEGIPSFSPEIFCWLRNPNPSAFKNFRK
HHCCCCCCCEEECCCCCCEEECCHHHCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHH
GVNELREEGAVQILYDFDESKRDPILAAVGQLQLDVVIHRLKNEYGVDANLDSMPYQLAR
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
WVSDGWSAIDELGRIFNCKVVKDCWNRPVILFKNQWNLNQFIEDNASFSLKKVSPVVSGV
HHHHHHHHHHHHHHHHCCEEHHHHHCCCEEEEECCCCHHHHHHCCCCEEHHHHHHHHCCC
EPIAL
CCCCC
>Mature Secondary Structure 
SSSTNNLNNKNILEAVNKRRNFAIISHPDAGKTTLTEKLLLYGGAIQQAGAVKARGNQR
CCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCCCC
KATSDWMDLEKQRGISITSTVLQFEYERAVINLLDTPGHKDFSEDTYRTLAAADNAVMLE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCEEEEH
DAAKGLEPQTRKLFEVCRMRKIPIFTFINKMDRPGREPFSLIDEIESELGLNTLPVNWPI
HHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
GMGEEFRGVIDRFTREVILFDKAVRGKQSNEKRMSLDDKELSKYVERELLDISLEELEVL
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHCCHHHHHHH
DEAGSKLEKEEIFKGSLTPVFFGSAMTNFGVRPFLDSFLKMAQKPTSRNSNKGDIEPASD
HHHHHHCCHHHHHCCCCCHHEECHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
EFSGFVFKLQANMDPKHRDRVAFIRVCSGKFEKDMSVKHSRTGKTIRLSRPQKIFGQERE
CCCCEEEEEECCCCCCHHHHEEEEEECCCCCCCCCCCHHCCCCCEEEECCCHHHHCCHHH
VVDDAYPGDVIGLNNPGMFSIGDTLYTGTFLEYEGIPSFSPEIFCWLRNPNPSAFKNFRK
HHCCCCCCCEEECCCCCCEEECCHHHCCEEEEECCCCCCCCCEEEEEECCCHHHHHHHHH
GVNELREEGAVQILYDFDESKRDPILAAVGQLQLDVVIHRLKNEYGVDANLDSMPYQLAR
HHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
WVSDGWSAIDELGRIFNCKVVKDCWNRPVILFKNQWNLNQFIEDNASFSLKKVSPVVSGV
HHHHHHHHHHHHHHHHCCEEHHHHHCCCEEEEECCCCHHHHHHCCCCEEHHHHHHHHCCC
EPIAL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA