Click here to switch to the map view.

The map label for this gene is menD

Identifier: 123965907

GI number: 123965907

Start: 606945

End: 608711

Strand: Direct

Name: menD

Synonym: P9515_06721

Alternate gene names: 123965907

Gene position: 606945-608711 (Clockwise)

Preceding gene: 123965904

Following gene: 123965908

Centisome position: 35.62

GC content: 28.58

Gene sequence:

>1767_bases
ATGACATCATCTATTGAATGCCAAAATATTTTTAGAAGTTTACAACTTTTAGATCTTTTTATAAAGATAGGAGTTAAAAA
TTTAATTTTATGTCCGGGAAGTCGATCAGCCCCTTTAGCAATAGCAGCAGGAGAGTTATATAAATATGGAATTTTAAATG
TATTTAATTCGATAGACGAAAGATCTGCTGGATTTCATTCACTTGGAATTTCTACTGCTTCGGGAGATATTTCATTAGTT
GTTACGACATCAGGGTCTGCCGTTGGAAACTTATTGCCTGCGGCCGTTGAGGCAGATAGATCCTGTAAATCAATCGTATT
TATAACTGCTGATAGACCCTTAAGGTTAAAAAATTGTGGTTCTAATCAAACAGTAAATCAAGAAGCATTTCTGAATTCAG
TTTGCAGAAGTACTTTGAGCACGAACTTAAATGGAATTCATAAGAATAATGACGATGATATTTTGAAAATAGTAAAAATG
ATAAAAAAACAATTGCTCCAATCGCCTGGCCCTATTCATTTAAATATTCCTTTTGAAAAACCTCTAGATATTTCTCTAAA
AAATAAAAGAAAGACTTTAAAGGTTTTTGAAACACTTTATTTGAATAAAACCTGTAAATTTTTAAAACAAAATGAACAAA
ATAAAAGTATTCATTTTTCTAAAAGAATTTTTGAAAGTTTAGATTTGTCTAACTCAGGAATAATTATTGTTGGTCCGTAT
CAGGGATCGATAAAAGATTTAGTTTCCTTTAATAATTCTTTAGAAAAAATACAATCAATTACAGGCTGGCCCGTTTTTGC
TGATCCTGTGTCAGGCGTATCAGCTGACTTGAGAGGATTAGTAGAAAATTGGGAATTAATTATTAAAAAAAATAATAACG
TTATCAAATGTAATCAGATTTTGAGACTAGGTCCTCTTTCCTCCTCAAACAATTTAGAGAAATTTTTATCAGATTTTGAT
GGCATACAAATTCTTGTCAAAGAAAATAATAGAAGAAAACTAGATCCTATAAAAAAATCTTTAGAATATGAATTTGGCTT
AACTAATTTTGTTAATCAACTTTTAGGAACCTATTCAGATGATCAAAAAAAGAAGAAACCTCTAATTAATTTGGTAAAAG
TTTTGAGGAGAGAAGGACAAAAAATTAGCAAAATTTTAAATGAACAAATTTTTTTAAATAAAGAAATAACAGAATATAAA
CTTGCAAATTTTGTACCTAAAATTTGGCCAGAAAACTATCCTATTATGCTTTCAGCAAGCAGTCCTATTAGAGATTGGCT
TACATTCTCTGAAAATGGAACTTTAACAAGGAAATGTTTTAGCTTTAGAGGGGCTTCAGGGATAGATGGGACTTTATCTC
TTGCATTAGGAATCGCTAGAATTACTCAACCTTTACTTTTGGTTACTGGGGATTTAGCCTTAATTCATGATATAAACGGT
TTCCTTATTGAAAATTCAATTGATTTAAATCTGACAGTTCTATTAATAAATAATAATGGTGGAAATATATTTAATAATTT
ATATAAAAATAATTTAGAAAAAGAAGAATTAAAGAAACTTTTTTTAATGCCTAAATCTATCAACTGGGAAAACCTTGCGA
AAGCTTTTCAAGTGCCAATTAAAATTGTTTCAGATTTAAATAAATTACCAGAATCTTTTGAATGGAGCCTTTCTATGCAA
AAATCAGTAATAATTAAAGTCGATATTAATGTTGATAATGAAATGAAAGAAAGAAGTTTAATCTTAAAAAAAATCCTCAA
TAATTAA

Upstream 100 bases:

>100_bases
AAATCATTTTTGATTGAACTTTTTTTTTCTAGTAGATTTGGCATTTTAGTTTAATTATTTATTTGTAAAAAAAGGAATTT
CTAAAGTATTTATATAATTT

Downstream 100 bases:

>100_bases
TGAAATTGCTTTTTAAAATTATTTAAATCTATGAAAATTTTACCTGGAAAAAATAACGCAGATTGGAAAGAATGGAAATC
CTATGAAGATATCTTGTTTC

Product: menaquinone biosynthesis protein

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD

Number of amino acids: Translated: 588; Mature: 587

Protein sequence:

>588_residues
MTSSIECQNIFRSLQLLDLFIKIGVKNLILCPGSRSAPLAIAAGELYKYGILNVFNSIDERSAGFHSLGISTASGDISLV
VTTSGSAVGNLLPAAVEADRSCKSIVFITADRPLRLKNCGSNQTVNQEAFLNSVCRSTLSTNLNGIHKNNDDDILKIVKM
IKKQLLQSPGPIHLNIPFEKPLDISLKNKRKTLKVFETLYLNKTCKFLKQNEQNKSIHFSKRIFESLDLSNSGIIIVGPY
QGSIKDLVSFNNSLEKIQSITGWPVFADPVSGVSADLRGLVENWELIIKKNNNVIKCNQILRLGPLSSSNNLEKFLSDFD
GIQILVKENNRRKLDPIKKSLEYEFGLTNFVNQLLGTYSDDQKKKKPLINLVKVLRREGQKISKILNEQIFLNKEITEYK
LANFVPKIWPENYPIMLSASSPIRDWLTFSENGTLTRKCFSFRGASGIDGTLSLALGIARITQPLLLVTGDLALIHDING
FLIENSIDLNLTVLLINNNGGNIFNNLYKNNLEKEELKKLFLMPKSINWENLAKAFQVPIKIVSDLNKLPESFEWSLSMQ
KSVIIKVDINVDNEMKERSLILKKILNN

Sequences:

>Translated_588_residues
MTSSIECQNIFRSLQLLDLFIKIGVKNLILCPGSRSAPLAIAAGELYKYGILNVFNSIDERSAGFHSLGISTASGDISLV
VTTSGSAVGNLLPAAVEADRSCKSIVFITADRPLRLKNCGSNQTVNQEAFLNSVCRSTLSTNLNGIHKNNDDDILKIVKM
IKKQLLQSPGPIHLNIPFEKPLDISLKNKRKTLKVFETLYLNKTCKFLKQNEQNKSIHFSKRIFESLDLSNSGIIIVGPY
QGSIKDLVSFNNSLEKIQSITGWPVFADPVSGVSADLRGLVENWELIIKKNNNVIKCNQILRLGPLSSSNNLEKFLSDFD
GIQILVKENNRRKLDPIKKSLEYEFGLTNFVNQLLGTYSDDQKKKKPLINLVKVLRREGQKISKILNEQIFLNKEITEYK
LANFVPKIWPENYPIMLSASSPIRDWLTFSENGTLTRKCFSFRGASGIDGTLSLALGIARITQPLLLVTGDLALIHDING
FLIENSIDLNLTVLLINNNGGNIFNNLYKNNLEKEELKKLFLMPKSINWENLAKAFQVPIKIVSDLNKLPESFEWSLSMQ
KSVIIKVDINVDNEMKERSLILKKILNN
>Mature_587_residues
TSSIECQNIFRSLQLLDLFIKIGVKNLILCPGSRSAPLAIAAGELYKYGILNVFNSIDERSAGFHSLGISTASGDISLVV
TTSGSAVGNLLPAAVEADRSCKSIVFITADRPLRLKNCGSNQTVNQEAFLNSVCRSTLSTNLNGIHKNNDDDILKIVKMI
KKQLLQSPGPIHLNIPFEKPLDISLKNKRKTLKVFETLYLNKTCKFLKQNEQNKSIHFSKRIFESLDLSNSGIIIVGPYQ
GSIKDLVSFNNSLEKIQSITGWPVFADPVSGVSADLRGLVENWELIIKKNNNVIKCNQILRLGPLSSSNNLEKFLSDFDG
IQILVKENNRRKLDPIKKSLEYEFGLTNFVNQLLGTYSDDQKKKKPLINLVKVLRREGQKISKILNEQIFLNKEITEYKL
ANFVPKIWPENYPIMLSASSPIRDWLTFSENGTLTRKCFSFRGASGIDGTLSLALGIARITQPLLLVTGDLALIHDINGF
LIENSIDLNLTVLLINNNGGNIFNNLYKNNLEKEELKKLFLMPKSINWENLAKAFQVPIKIVSDLNKLPESFEWSLSMQK
SVIIKVDINVDNEMKERSLILKKILNN

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily

Homologues:

Organism=Escherichia coli, GI1788599, Length=581, Percent_Identity=23.7521514629948, Blast_Score=162, Evalue=8e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MEND_PROM5 (A2BVS0)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010988.1
- ProteinModelPortal:   A2BVS0
- STRING:   A2BVS0
- GeneID:   4719550
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_06721
- eggNOG:   COG1165
- HOGENOM:   HBG634405
- OMA:   FYHDLNG
- ProtClustDB:   CLSK921906
- BioCyc:   PMAR167542:P9515ORF_0708-MONOMER
- HAMAP:   MF_01659
- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766
- TIGRFAMs:   TIGR00173

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N

EC number: =2.2.1.9

Molecular weight: Translated: 65896; Mature: 65765

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSSIECQNIFRSLQLLDLFIKIGVKNLILCPGSRSAPLAIAAGELYKYGILNVFNSIDE
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHH
RSAGFHSLGISTASGDISLVVTTSGSAVGNLLPAAVEADRSCKSIVFITADRPLRLKNCG
HCCCCEECCCEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCC
SNQTVNQEAFLNSVCRSTLSTNLNGIHKNNDDDILKIVKMIKKQLLQSPGPIHLNIPFEK
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCC
PLDISLKNKRKTLKVFETLYLNKTCKFLKQNEQNKSIHFSKRIFESLDLSNSGIIIVGPY
CCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECC
QGSIKDLVSFNNSLEKIQSITGWPVFADPVSGVSADLRGLVENWELIIKKNNNVIKCNQI
CCCHHHHHHHCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCEEEEECCCCEEEECCE
LRLGPLSSSNNLEKFLSDFDGIQILVKENNRRKLDPIKKSLEYEFGLTNFVNQLLGTYSD
EEECCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCC
DQKKKKPLINLVKVLRREGQKISKILNEQIFLNKEITEYKLANFVPKIWPENYPIMLSAS
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCEEEECC
SPIRDWLTFSENGTLTRKCFSFRGASGIDGTLSLALGIARITQPLLLVTGDLALIHDING
CCHHHHHEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCEEEEECCCC
FLIENSIDLNLTVLLINNNGGNIFNNLYKNNLEKEELKKLFLMPKSINWENLAKAFQVPI
EEEECCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
KIVSDLNKLPESFEWSLSMQKSVIIKVDINVDNEMKERSLILKKILNN
HHHHHHHHCCCHHEECEEECCEEEEEEEECCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSSIECQNIFRSLQLLDLFIKIGVKNLILCPGSRSAPLAIAAGELYKYGILNVFNSIDE
CCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEECCHHHHHHHHHHHHHHHH
RSAGFHSLGISTASGDISLVVTTSGSAVGNLLPAAVEADRSCKSIVFITADRPLRLKNCG
HCCCCEECCCEECCCCEEEEEEECCCHHHHHHHHHHHCCCCCCEEEEEECCCCEEECCCC
SNQTVNQEAFLNSVCRSTLSTNLNGIHKNNDDDILKIVKMIKKQLLQSPGPIHLNIPFEK
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCC
PLDISLKNKRKTLKVFETLYLNKTCKFLKQNEQNKSIHFSKRIFESLDLSNSGIIIVGPY
CCCCEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEECC
QGSIKDLVSFNNSLEKIQSITGWPVFADPVSGVSADLRGLVENWELIIKKNNNVIKCNQI
CCCHHHHHHHCHHHHHHHHHCCCCEECCCCCCCHHHHHHHHHCCEEEEECCCCEEEECCE
LRLGPLSSSNNLEKFLSDFDGIQILVKENNRRKLDPIKKSLEYEFGLTNFVNQLLGTYSD
EEECCCCCCCCHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCC
DQKKKKPLINLVKVLRREGQKISKILNEQIFLNKEITEYKLANFVPKIWPENYPIMLSAS
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHEECCCHHHHHHHHHHHHHCCCCCCEEEECC
SPIRDWLTFSENGTLTRKCFSFRGASGIDGTLSLALGIARITQPLLLVTGDLALIHDING
CCHHHHHEECCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEECCEEEEECCCC
FLIENSIDLNLTVLLINNNGGNIFNNLYKNNLEKEELKKLFLMPKSINWENLAKAFQVPI
EEEECCCCEEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
KIVSDLNKLPESFEWSLSMQKSVIIKVDINVDNEMKERSLILKKILNN
HHHHHHHHCCCHHEECEEECCEEEEEEEECCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA