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The map label for this gene is cocE [H]

Identifier: 123965885

GI number: 123965885

Start: 582440

End: 584017

Strand: Reverse

Name: cocE [H]

Synonym: P9515_06501

Alternate gene names: 123965885

Gene position: 584017-582440 (Counterclockwise)

Preceding gene: 123965886

Following gene: 123965884

Centisome position: 34.27

GC content: 34.41

Gene sequence:

>1578_bases
ATGCGGGAAGTAAATTGGGTTGATAAGTCATTAAGACTAAATGATGGGATAGAACTAGCTTCAAGAATTTGGACCCCTAA
AGGAAACGGTTCTTGGCCCGCCTTATTAATGAGACAACCATATGGAAGAGAAATAGCTTCAACAATTACTTATTCTCATC
CTGAATGGTGGGCTTCAAAAGGTTACATGGTCGTAATTCAAGATGTAAGAGGTCAGGGTTCATCTAGTGGAATATTTAAA
GGATTTTCTCAAGAACCTAAAGACACCTCAGAAACACATCAATGGGTAAGATCTTTAAAAGAATGCAATGGAAAACTTGG
CTTATATGGCTTTTCATATCAAGGATTGACTCAACTTACAGGAACCAAAGATTCAAAGCCTCCCGATTGCTTATCCCCCG
CAATGACGGGAATAGATTTAAAAAATCACTGGAGTTCGGATGGTGGGGCTTTTTGGTGGAATAATAATATTGCGTGGGGA
TTACAAATAGCTGCCTTAAAGATGAAAAGAGAAAATAATTCTTTAGGATGGGAAGAAATAATTTCAGCGCTGGAAAATAA
AAGTTATTTAAGAAAAGGATTAGCTCTTTTAAAAAAATACGATCCTAATAACTTTGTACTTGAATGGCATAAAAATATAA
ATCATGATAATGATTTTCTTGAGATAAAGTTAATTTCATCATGGTTAAAAAAACCAATGTTAATTATTGGTGGTCTTTGG
GATCCTCATCTAAGAGGTGCATTTGACCTCTATCAAAAGTCAAAAGCTGCAGGAGGAAATCCTGACATAATAATTGGTGA
TGCTACACACTTGAATTGGTGGGAGGGATCACAAAAAACTTTATTAAACTTTTTTGATACCCACCTCAAAAGTGATAAAG
AAGTTAAAGAAAAAAATTTTTCTAAACATAAAAAAATTTGGAATCTTTCATTAAAAAAATGGGAACCAATTGAAGAGGGA
AAGGTTCAAAAATATAAGTTTGGCCTTGAAAGTAATGGGACCGCAAATACTGAAGTCACTGATGGTAGTCTTCTGCTAAA
TTCAAAGGGTTCAGGCTGGTTTTCAATTGTTAATGATCCATGGAGGCCGTCTCAAGGAGAGGGAGGACATTTAGGTCCAA
CCCCTGGGATGTTTGATAGATATATTTTTGATACAAGATTAGATGTTGGGGTTTTTCAAACCAATTCTCTGCAGAAAGAT
CTTCAGATTTCAGGAATTCCAACCCTCGAAACCTCAGTAAAATGTGATCAGAAAAGCTTTGATATATGTCTTGCCTTGTC
TATGGTTAATGAAAAAGAAAAAACTGTCAATCAATTTTCAACTGGATTTTTAAGAGTTAAAAACTCAAAGAAAAATGAAA
AATGTGAGTGCAAAATTGAAATGCATCCAACAAATATTCTACTTCTTAAGGGTTCAAAACTTAGGTTGTCAATATCAGCT
GCGGCATACCCAGCAATTGGCATAAATTCAGGATCTAGCGACAATAATAATGGAGCTCCCACAATTAATCACAAAATTAT
AACTTTAAGTTTTGAACTCAGTAAAACATTTATGAAAATGAATCCATTTTTCAATTGA

Upstream 100 bases:

>100_bases
AGTTTGTTGCAAAAAAAAATAACCTAAACCCAATTAATGCTGAGATTCTCTCTATTTATGTAATTGGACTTCATTGCCCT
CAGGTTATTTAAATAATTAA

Downstream 100 bases:

>100_bases
TTTTTTTTTTGTTGATAATTTGATAAACTTATCTCTTAACTGCTACCAGTCATGAAAGAAACTATTCAAACAGACTGGAT
TAAATCAGAAGCTTTCAACC

Product: acyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MREVNWVDKSLRLNDGIELASRIWTPKGNGSWPALLMRQPYGREIASTITYSHPEWWASKGYMVVIQDVRGQGSSSGIFK
GFSQEPKDTSETHQWVRSLKECNGKLGLYGFSYQGLTQLTGTKDSKPPDCLSPAMTGIDLKNHWSSDGGAFWWNNNIAWG
LQIAALKMKRENNSLGWEEIISALENKSYLRKGLALLKKYDPNNFVLEWHKNINHDNDFLEIKLISSWLKKPMLIIGGLW
DPHLRGAFDLYQKSKAAGGNPDIIIGDATHLNWWEGSQKTLLNFFDTHLKSDKEVKEKNFSKHKKIWNLSLKKWEPIEEG
KVQKYKFGLESNGTANTEVTDGSLLLNSKGSGWFSIVNDPWRPSQGEGGHLGPTPGMFDRYIFDTRLDVGVFQTNSLQKD
LQISGIPTLETSVKCDQKSFDICLALSMVNEKEKTVNQFSTGFLRVKNSKKNEKCECKIEMHPTNILLLKGSKLRLSISA
AAYPAIGINSGSSDNNNGAPTINHKIITLSFELSKTFMKMNPFFN

Sequences:

>Translated_525_residues
MREVNWVDKSLRLNDGIELASRIWTPKGNGSWPALLMRQPYGREIASTITYSHPEWWASKGYMVVIQDVRGQGSSSGIFK
GFSQEPKDTSETHQWVRSLKECNGKLGLYGFSYQGLTQLTGTKDSKPPDCLSPAMTGIDLKNHWSSDGGAFWWNNNIAWG
LQIAALKMKRENNSLGWEEIISALENKSYLRKGLALLKKYDPNNFVLEWHKNINHDNDFLEIKLISSWLKKPMLIIGGLW
DPHLRGAFDLYQKSKAAGGNPDIIIGDATHLNWWEGSQKTLLNFFDTHLKSDKEVKEKNFSKHKKIWNLSLKKWEPIEEG
KVQKYKFGLESNGTANTEVTDGSLLLNSKGSGWFSIVNDPWRPSQGEGGHLGPTPGMFDRYIFDTRLDVGVFQTNSLQKD
LQISGIPTLETSVKCDQKSFDICLALSMVNEKEKTVNQFSTGFLRVKNSKKNEKCECKIEMHPTNILLLKGSKLRLSISA
AAYPAIGINSGSSDNNNGAPTINHKIITLSFELSKTFMKMNPFFN
>Mature_525_residues
MREVNWVDKSLRLNDGIELASRIWTPKGNGSWPALLMRQPYGREIASTITYSHPEWWASKGYMVVIQDVRGQGSSSGIFK
GFSQEPKDTSETHQWVRSLKECNGKLGLYGFSYQGLTQLTGTKDSKPPDCLSPAMTGIDLKNHWSSDGGAFWWNNNIAWG
LQIAALKMKRENNSLGWEEIISALENKSYLRKGLALLKKYDPNNFVLEWHKNINHDNDFLEIKLISSWLKKPMLIIGGLW
DPHLRGAFDLYQKSKAAGGNPDIIIGDATHLNWWEGSQKTLLNFFDTHLKSDKEVKEKNFSKHKKIWNLSLKKWEPIEEG
KVQKYKFGLESNGTANTEVTDGSLLLNSKGSGWFSIVNDPWRPSQGEGGHLGPTPGMFDRYIFDTRLDVGVFQTNSLQKD
LQISGIPTLETSVKCDQKSFDICLALSMVNEKEKTVNQFSTGFLRVKNSKKNEKCECKIEMHPTNILLLKGSKLRLSISA
AAYPAIGINSGSSDNNNGAPTINHKIITLSFELSKTFMKMNPFFN

Specific function: Hydrolyzes cocaine to benzoate and ecgonine methyl ester, endowing the bacteria with the ability to utilize cocaine as a sole source of carbon and energy for growth, as this bacterium lives in the rhizosphere of coca plants [H]

COG id: COG2936

COG function: function code R; Predicted acyl esterases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha/beta hydrolase family. Carboxylesterase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005674
- InterPro:   IPR008979
- InterPro:   IPR013736
- InterPro:   IPR000383 [H]

Pfam domain/function: PF02129 Peptidase_S15; PF08530 PepX_C [H]

EC number: NA

Molecular weight: Translated: 59220; Mature: 59220

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREVNWVDKSLRLNDGIELASRIWTPKGNGSWPALLMRQPYGREIASTITYSHPEWWASK
CCCCCCCCCCEECCCCHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHEECCCCHHHCCC
GYMVVIQDVRGQGSSSGIFKGFSQEPKDTSETHQWVRSLKECNGKLGLYGFSYQGLTQLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHEEC
GTKDSKPPDCLSPAMTGIDLKNHWSSDGGAFWWNNNIAWGLQIAALKMKRENNSLGWEEI
CCCCCCCCHHHCHHHCCCCHHHCCCCCCCEEEECCCEEEEEEEEEEEEECCCCCCCHHHH
ISALENKSYLRKGLALLKKYDPNNFVLEWHKNINHDNDFLEIKLISSWLKKPMLIIGGLW
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEHHHHHHHCCCEEEEECCC
DPHLRGAFDLYQKSKAAGGNPDIIIGDATHLNWWEGSQKTLLNFFDTHLKSDKEVKEKNF
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
SKHKKIWNLSLKKWEPIEEGKVQKYKFGLESNGTANTEVTDGSLLLNSKGSGWFSIVNDP
HHHHHEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCEECCCEEEEECCCCCEEEECCCC
WRPSQGEGGHLGPTPGMFDRYIFDTRLDVGVFQTNSLQKDLQISGIPTLETSVKCDQKSF
CCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCC
DICLALSMVNEKEKTVNQFSTGFLRVKNSKKNEKCECKIEMHPTNILLLKGSKLRLSISA
CEEEEEEHHCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCEEEEEECCEEEEEEEC
AAYPAIGINSGSSDNNNGAPTINHKIITLSFELSKTFMKMNPFFN
CCCCEEECCCCCCCCCCCCCEECEEEEEEEEEHHHHHHHCCCCCC
>Mature Secondary Structure
MREVNWVDKSLRLNDGIELASRIWTPKGNGSWPALLMRQPYGREIASTITYSHPEWWASK
CCCCCCCCCCEECCCCHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHEECCCCHHHCCC
GYMVVIQDVRGQGSSSGIFKGFSQEPKDTSETHQWVRSLKECNGKLGLYGFSYQGLTQLT
CEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHEEC
GTKDSKPPDCLSPAMTGIDLKNHWSSDGGAFWWNNNIAWGLQIAALKMKRENNSLGWEEI
CCCCCCCCHHHCHHHCCCCHHHCCCCCCCEEEECCCEEEEEEEEEEEEECCCCCCCHHHH
ISALENKSYLRKGLALLKKYDPNNFVLEWHKNINHDNDFLEIKLISSWLKKPMLIIGGLW
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCEEEEHHHHHHHCCCEEEEECCC
DPHLRGAFDLYQKSKAAGGNPDIIIGDATHLNWWEGSQKTLLNFFDTHLKSDKEVKEKNF
CCHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
SKHKKIWNLSLKKWEPIEEGKVQKYKFGLESNGTANTEVTDGSLLLNSKGSGWFSIVNDP
HHHHHEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCEECCCEEEEECCCCCEEEECCCC
WRPSQGEGGHLGPTPGMFDRYIFDTRLDVGVFQTNSLQKDLQISGIPTLETSVKCDQKSF
CCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCC
DICLALSMVNEKEKTVNQFSTGFLRVKNSKKNEKCECKIEMHPTNILLLKGSKLRLSISA
CEEEEEEHHCHHHHHHHHHCCCEEEEECCCCCCCEEEEEEECCCEEEEEECCEEEEEEEC
AAYPAIGINSGSSDNNNGAPTINHKIITLSFELSKTFMKMNPFFN
CCCCEEECCCCCCCCCCCCCEECEEEEEEEEEHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10698749; 11742345; 12369817 [H]