Click here to switch to the map view.

The map label for this gene is glgB

Identifier: 123965884

GI number: 123965884

Start: 580124

End: 582388

Strand: Reverse

Name: glgB

Synonym: P9515_06491

Alternate gene names: 123965884

Gene position: 582388-580124 (Counterclockwise)

Preceding gene: 123965885

Following gene: 123965883

Centisome position: 34.17

GC content: 34.88

Gene sequence:

>2265_bases
ATGAAAGAAACTATTCAAACAGACTGGATTAAATCAGAAGCTTTCAACCTCGAAAATTGTTGCAATGATAATCCATTAAA
TATTTTAGGTCCCCATTTCTTCAAAGGGAAGTGGGTTACAAGAGTATGGATGCCAGAGGCAGATGAAGTAAATATTACTT
TTAAGGATAAAACCTATAAAACAACTACTCCCAATCATAAATGGATGTTTGAGGCAATTCTTCATGAAGACCCAAAAAAT
AATTATCAAATAAATGTATTGAGAGGAGGAGTTTCTCATTCACAACAAGACCCATGGGCTTTTAAGGATGAATGGATGGG
GGAGGTCGATAGGCACCTTTTTGCAGAGGGTAATCACCATCATATTTGGAAGAAAATGGGTGCCCATATCAGTGAAGATA
TAAATCAAAAAGGTGTAATGTTCTGTATTTGGGCACCAAATGCAAAATCAATCTCAATAATCGGAGATATAAATTCTTGG
GACGGTAGACATCATCCTATGCAAAAAAGGCTGGGTGGAGTTTGGGAATTATTTATGCCAATATTGAAGGAAGGAGATGT
TTATAAATATGAAATAAGAACTCAACAAGGGCATATTTATGAAAAAGCAGACCCATATGGTTTTCTTCATGAAGTAAGGC
CCCAAAATGGCTCAATAATATCTAAACTCAACAACTTCAATTGGGAAGATAGTTCTTGGATCACTAGTAGAGATTCATCA
AGTCAAATTAACAAACCTATTTCAGTTTATGAGATGCATCTTGGAAGTTGGATGCATGATTCAACCGATAATAAGTATAT
TGAAAAAAATGGCAACCCAAGACATCCAGTACCCGCAGCAGATTTAAAGCCTGGGACAAGGTTTTTAACTTATCCTGAAT
TAACCGAAAAGCTTATCCCATATGTGAAAGAAAGAGGATTTACTCATATCGAATTAATGCCTATTTCAGAACATCCTTTT
GATGGTTCATGGGGATATCAAGTTACAGGTTGGTATGCCCCAACAAGTAGATATGGAACTCCAAATGAATTTAAAGAGTT
TATAAATAAATGCCATGCAGAGGGCATAGGTGTCATTCTAGACTGGGTTCCTGGCCATTTCCCAAAAGATAAACATGGTT
TAGCTTTCTTTGATGGTTGTCATCTTTATGAGCATGGTGATCCTCGTATTGGAGAACATAAAGAATGGGGAACTTTAATT
TTTAACTACAGTAGAAATGAAGTAAGGAATTTTTTAGTAGCAAATCTTATTTATTGGTTTGAAGAATTTCATATTGATGG
AATAAGAGTAGATGCTGTAGCTTCAATGTTATACAGAGATTATTTACGTCCAGAAGGAGAATGGATTCCAAATGAAAATG
GTGGAAATGAAAATTTAGAAGCTGTTAAATTTCTTCAGCAAGCAAATCATGTCCTTTTTCAACATTTCCCGGGAGCACTA
TCTATTGCTGAAGAATCCACTACTTGGCCAATGGTTACAGAGCCAACAAATGTAGGAGGTTTAGGTTTCAATTTAAAGTG
GAATATGGGGTGGATGCACGATATGCTCGATTATTTTGAAATAGACCCTTGGTTTAGACAATTTCATCAAAATAGCGTGA
CCTTTTCGATAACTTATAACTATACCGAAAATTTTATGCTTGCTCTTAGCCATGATGAGGTTGTTCATGGGAAAAGTCAC
CTTCTACATAAAATGCCAGGAGATGATTGGAAAAAATTTGCGAATACAAGAGCTTTATTAACTTATATGTGGACACATCC
TGGTAAAAAGACAATTTTTATGGGAATGGAATTTGGACAAAGGCAAGAATGGAATGTTTGGGATGATCTTCAATGGGAAC
TTCTAGAATATGAACCCCACAAAGGTATTAGAAACCTTATTGATGACTTAAATAAACTTTATAAAAATGAGCCATCTTTA
TGGAAAAATGACTTTGATCCTTATGGATTTCAATGGATAGATTGCGATGACACCTCTAATTCAGTTATTAGTTTTATGAG
AAGGGAAAATGAAAGTAATGAATGGCTTGTGGTTATAGCAAACTTTACCCCTAATTTTCACGAATCATATAAGATAGGTG
TTCCATTAGAAGGATACTATAAGGAGATTTTTAATTCAGATGGATCTAAATATGGAGGAAGTAACAAAGGGAATATGGGA
GGTAAGGACACCCTAAAATATAATATTCACAATTATGAAAATGCTCTTGAAATTGCTCTCCCACCACTAAGTGTAAGTAT
CTTTAAGCATCAGCTAAAGAAATAA

Upstream 100 bases:

>100_bases
TTTTGAACTCAGTAAAACATTTATGAAAATGAATCCATTTTTCAATTGATTTTTTTTTTGTTGATAATTTGATAAACTTA
TCTCTTAACTGCTACCAGTC

Downstream 100 bases:

>100_bases
AAAAGCTTTTTAATTAGTTAATTTCATTTAATTCTTCACATCAACGATTTTAATCTGCTCCACGTTGTAAACTTTTTAAG
TTAAAGATTTTTTATTTTTT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 754; Mature: 754

Protein sequence:

>754_residues
MKETIQTDWIKSEAFNLENCCNDNPLNILGPHFFKGKWVTRVWMPEADEVNITFKDKTYKTTTPNHKWMFEAILHEDPKN
NYQINVLRGGVSHSQQDPWAFKDEWMGEVDRHLFAEGNHHHIWKKMGAHISEDINQKGVMFCIWAPNAKSISIIGDINSW
DGRHHPMQKRLGGVWELFMPILKEGDVYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIISKLNNFNWEDSSWITSRDSS
SQINKPISVYEMHLGSWMHDSTDNKYIEKNGNPRHPVPAADLKPGTRFLTYPELTEKLIPYVKERGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRYGTPNEFKEFINKCHAEGIGVILDWVPGHFPKDKHGLAFFDGCHLYEHGDPRIGEHKEWGTLI
FNYSRNEVRNFLVANLIYWFEEFHIDGIRVDAVASMLYRDYLRPEGEWIPNENGGNENLEAVKFLQQANHVLFQHFPGAL
SIAEESTTWPMVTEPTNVGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYNYTENFMLALSHDEVVHGKSH
LLHKMPGDDWKKFANTRALLTYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWELLEYEPHKGIRNLIDDLNKLYKNEPSL
WKNDFDPYGFQWIDCDDTSNSVISFMRRENESNEWLVVIANFTPNFHESYKIGVPLEGYYKEIFNSDGSKYGGSNKGNMG
GKDTLKYNIHNYENALEIALPPLSVSIFKHQLKK

Sequences:

>Translated_754_residues
MKETIQTDWIKSEAFNLENCCNDNPLNILGPHFFKGKWVTRVWMPEADEVNITFKDKTYKTTTPNHKWMFEAILHEDPKN
NYQINVLRGGVSHSQQDPWAFKDEWMGEVDRHLFAEGNHHHIWKKMGAHISEDINQKGVMFCIWAPNAKSISIIGDINSW
DGRHHPMQKRLGGVWELFMPILKEGDVYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIISKLNNFNWEDSSWITSRDSS
SQINKPISVYEMHLGSWMHDSTDNKYIEKNGNPRHPVPAADLKPGTRFLTYPELTEKLIPYVKERGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRYGTPNEFKEFINKCHAEGIGVILDWVPGHFPKDKHGLAFFDGCHLYEHGDPRIGEHKEWGTLI
FNYSRNEVRNFLVANLIYWFEEFHIDGIRVDAVASMLYRDYLRPEGEWIPNENGGNENLEAVKFLQQANHVLFQHFPGAL
SIAEESTTWPMVTEPTNVGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYNYTENFMLALSHDEVVHGKSH
LLHKMPGDDWKKFANTRALLTYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWELLEYEPHKGIRNLIDDLNKLYKNEPSL
WKNDFDPYGFQWIDCDDTSNSVISFMRRENESNEWLVVIANFTPNFHESYKIGVPLEGYYKEIFNSDGSKYGGSNKGNMG
GKDTLKYNIHNYENALEIALPPLSVSIFKHQLKK
>Mature_754_residues
MKETIQTDWIKSEAFNLENCCNDNPLNILGPHFFKGKWVTRVWMPEADEVNITFKDKTYKTTTPNHKWMFEAILHEDPKN
NYQINVLRGGVSHSQQDPWAFKDEWMGEVDRHLFAEGNHHHIWKKMGAHISEDINQKGVMFCIWAPNAKSISIIGDINSW
DGRHHPMQKRLGGVWELFMPILKEGDVYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIISKLNNFNWEDSSWITSRDSS
SQINKPISVYEMHLGSWMHDSTDNKYIEKNGNPRHPVPAADLKPGTRFLTYPELTEKLIPYVKERGFTHIELMPISEHPF
DGSWGYQVTGWYAPTSRYGTPNEFKEFINKCHAEGIGVILDWVPGHFPKDKHGLAFFDGCHLYEHGDPRIGEHKEWGTLI
FNYSRNEVRNFLVANLIYWFEEFHIDGIRVDAVASMLYRDYLRPEGEWIPNENGGNENLEAVKFLQQANHVLFQHFPGAL
SIAEESTTWPMVTEPTNVGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYNYTENFMLALSHDEVVHGKSH
LLHKMPGDDWKKFANTRALLTYMWTHPGKKTIFMGMEFGQRQEWNVWDDLQWELLEYEPHKGIRNLIDDLNKLYKNEPSL
WKNDFDPYGFQWIDCDDTSNSVISFMRRENESNEWLVVIANFTPNFHESYKIGVPLEGYYKEIFNSDGSKYGGSNKGNMG
GKDTLKYNIHNYENALEIALPPLSVSIFKHQLKK

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=658, Percent_Identity=27.0516717325228, Blast_Score=202, Evalue=1e-51,
Organism=Escherichia coli, GI1789839, Length=730, Percent_Identity=46.3013698630137, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=647, Percent_Identity=25.8114374034003, Blast_Score=172, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI32564391, Length=619, Percent_Identity=25.3634894991922, Blast_Score=148, Evalue=9e-36,
Organism=Saccharomyces cerevisiae, GI6320826, Length=722, Percent_Identity=26.4542936288089, Blast_Score=181, Evalue=3e-46,
Organism=Drosophila melanogaster, GI28573410, Length=680, Percent_Identity=26.3235294117647, Blast_Score=201, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_PROM5 (A2BVP7)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010965.1
- ProteinModelPortal:   A2BVP7
- STRING:   A2BVP7
- GeneID:   4719658
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_06491
- eggNOG:   COG0296
- HOGENOM:   HBG287139
- OMA:   DGTCLYE
- ProtClustDB:   PRK05402
- BioCyc:   PMAR167542:P9515ORF_0685-MONOMER
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 88201; Mature: 88201

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: ACT_SITE 326-326 ACT_SITE 361-361 ACT_SITE 366-366 ACT_SITE 429-429 ACT_SITE 431-431 ACT_SITE 484-484 ACT_SITE 551-551 ACT_SITE 552-552

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETIQTDWIKSEAFNLENCCNDNPLNILGPHFFKGKWVTRVWMPEADEVNITFKDKTYK
CCCCCCCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEE
TTTPNHKWMFEAILHEDPKNNYQINVLRGGVSHSQQDPWAFKDEWMGEVDRHLFAEGNHH
CCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
HIWKKMGAHISEDINQKGVMFCIWAPNAKSISIIGDINSWDGRHHPMQKRLGGVWELFMP
HHHHHHCCHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
ILKEGDVYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIISKLNNFNWEDSSWITSRDSS
HHCCCCEEEEEEEECCCCEEECCCCCCHHHHCCCCCCCHHHHHCCCCCCCCCEEECCCCC
SQINKPISVYEMHLGSWMHDSTDNKYIEKNGNPRHPVPAADLKPGTRFLTYPELTEKLIP
HHCCCCCEEEEEHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
YVKERGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRYGTPNEFKEFINKCHAEGIGVIL
HHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEE
DWVPGHFPKDKHGLAFFDGCHLYEHGDPRIGEHKEWGTLIFNYSRNEVRNFLVANLIYWF
ECCCCCCCCCCCCEEEEECEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
EEFHIDGIRVDAVASMLYRDYLRPEGEWIPNENGGNENLEAVKFLQQANHVLFQHFPGAL
HHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
SIAEESTTWPMVTEPTNVGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYN
EECCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEEE
YTENFMLALSHDEVVHGKSHLLHKMPGDDWKKFANTRALLTYMWTHPGKKTIFMGMEFGQ
CCCCEEEEECCCCHHCCHHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCC
RQEWNVWDDLQWELLEYEPHKGIRNLIDDLNKLYKNEPSLWKNDFDPYGFQWIDCDDTSN
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHH
SVISFMRRENESNEWLVVIANFTPNFHESYKIGVPLEGYYKEIFNSDGSKYGGSNKGNMG
HHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCCC
GKDTLKYNIHNYENALEIALPPLSVSIFKHQLKK
CCCEEEEEECCCCCEEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MKETIQTDWIKSEAFNLENCCNDNPLNILGPHFFKGKWVTRVWMPEADEVNITFKDKTYK
CCCCCCCHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEEECCCEEE
TTTPNHKWMFEAILHEDPKNNYQINVLRGGVSHSQQDPWAFKDEWMGEVDRHLFAEGNHH
CCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
HIWKKMGAHISEDINQKGVMFCIWAPNAKSISIIGDINSWDGRHHPMQKRLGGVWELFMP
HHHHHHCCHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHH
ILKEGDVYKYEIRTQQGHIYEKADPYGFLHEVRPQNGSIISKLNNFNWEDSSWITSRDSS
HHCCCCEEEEEEEECCCCEEECCCCCCHHHHCCCCCCCHHHHHCCCCCCCCCEEECCCCC
SQINKPISVYEMHLGSWMHDSTDNKYIEKNGNPRHPVPAADLKPGTRFLTYPELTEKLIP
HHCCCCCEEEEEHHCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEECHHHHHHHHH
YVKERGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRYGTPNEFKEFINKCHAEGIGVIL
HHHHCCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEE
DWVPGHFPKDKHGLAFFDGCHLYEHGDPRIGEHKEWGTLIFNYSRNEVRNFLVANLIYWF
ECCCCCCCCCCCCEEEEECEEEEECCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHH
EEFHIDGIRVDAVASMLYRDYLRPEGEWIPNENGGNENLEAVKFLQQANHVLFQHFPGAL
HHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
SIAEESTTWPMVTEPTNVGGLGFNLKWNMGWMHDMLDYFEIDPWFRQFHQNSVTFSITYN
EECCCCCCCCEEECCCCCCCEEEEEEECCCHHHHHHHHHCCCHHHHHHCCCCEEEEEEEE
YTENFMLALSHDEVVHGKSHLLHKMPGDDWKKFANTRALLTYMWTHPGKKTIFMGMEFGQ
CCCCEEEEECCCCHHCCHHHHHHCCCCHHHHHHHCCCEEEEEEEECCCCEEEEEECCCCC
RQEWNVWDDLQWELLEYEPHKGIRNLIDDLNKLYKNEPSLWKNDFDPYGFQWIDCDDTSN
CCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCHH
SVISFMRRENESNEWLVVIANFTPNFHESYKIGVPLEGYYKEIFNSDGSKYGGSNKGNMG
HHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCHHHHHHHHCCCCCCCCCCCCCCCC
GKDTLKYNIHNYENALEIALPPLSVSIFKHQLKK
CCCEEEEEECCCCCEEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA