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The map label for this gene is trpA [H]

Identifier: 123965872

GI number: 123965872

Start: 570010

End: 570849

Strand: Direct

Name: trpA [H]

Synonym: P9515_06371

Alternate gene names: 123965872

Gene position: 570010-570849 (Clockwise)

Preceding gene: 123965871

Following gene: 123965874

Centisome position: 33.45

GC content: 31.43

Gene sequence:

>840_bases
ATGAAAGAAAACAAGAAACCTCAATTTACTAAAAATGAAACTTTATCAAAAATAAATGAGAAGTTTTTGGAATTAAAGAA
AAGTAAAAAATTAGCCTTGATGCCTTTTATAATGGCTGGTGATCCAAATATTGAAAAAACATCAGAGATTTTATTAAAGT
TGCAAGAAAAGGGTGCAGATCTTATTGAATTGGGAATCCCTTATAGTGACCCTCTTGCAGATGGACCGATTATACAATTT
TCAGCATCTAGGGCTTTAAAATCAGGAACTACTTCCATAAAGGTTATTCAGTTATTAGAGTCGCTTAAAGATAAGTTACG
TATTCCAGTTATACTTTTTACTTATTTTAATCCTATCTTAAATTTTGGACTTGAGAATTTTTGTGATTTAGCATCCAAAG
CAGGTGTTGCAGGATTGATAATTCCTGATCTTCCCTTAGAAGAAGCCTCTAAATTTTCGGAAATTATTAGTTCTTATTTT
ATTGATTTAATATTATTAGTTGCCCCTACTACACCCTTTGAAAGAATGAAAATGATATCTAACAAAACAAAAGGGTTTAC
TTATCTTGTAAGCGTAACAGGAGTAACAGGAGAAAGAAGCAAAATGGGAAATAGAGTAGAAAGTCTTATTACTAAATTAA
AAGAAATAAGCATTAATCCAGTAGCAGTTGGTTTTGGAATATCCGCTCCTGAACATGTTAATAGAGTCAGTAAATGGGGG
GCAGATGGAGTAATTATTGGAAGCGCATTTGTTAAAAGAATTTCTAATAGTAATGAAAGAGAAGTTGTAAATGAAGTAGG
AAACTTTTGCGAAGAAATGCGTAAAGCAGCTGATCAATAA

Upstream 100 bases:

>100_bases
CAACTCATTACCAAAAGAAGAGCAGGAGAAACTAATGGAAGATTTAGAAAAAAATCTATAAAACACTTAACAATAATCAA
ATAAAAAAAATCAAAAATTC

Downstream 100 bases:

>100_bases
TAGTAAATAAGAAAAAAATTTTTTTTATAAACTTATATGATTCAAAATCATTAGCGTAATAGGGGAGTATTTATTTTTTT
ATAGTTAAACCAATTCTTTA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MKENKKPQFTKNETLSKINEKFLELKKSKKLALMPFIMAGDPNIEKTSEILLKLQEKGADLIELGIPYSDPLADGPIIQF
SASRALKSGTTSIKVIQLLESLKDKLRIPVILFTYFNPILNFGLENFCDLASKAGVAGLIIPDLPLEEASKFSEIISSYF
IDLILLVAPTTPFERMKMISNKTKGFTYLVSVTGVTGERSKMGNRVESLITKLKEISINPVAVGFGISAPEHVNRVSKWG
ADGVIIGSAFVKRISNSNEREVVNEVGNFCEEMRKAADQ

Sequences:

>Translated_279_residues
MKENKKPQFTKNETLSKINEKFLELKKSKKLALMPFIMAGDPNIEKTSEILLKLQEKGADLIELGIPYSDPLADGPIIQF
SASRALKSGTTSIKVIQLLESLKDKLRIPVILFTYFNPILNFGLENFCDLASKAGVAGLIIPDLPLEEASKFSEIISSYF
IDLILLVAPTTPFERMKMISNKTKGFTYLVSVTGVTGERSKMGNRVESLITKLKEISINPVAVGFGISAPEHVNRVSKWG
ADGVIIGSAFVKRISNSNEREVVNEVGNFCEEMRKAADQ
>Mature_279_residues
MKENKKPQFTKNETLSKINEKFLELKKSKKLALMPFIMAGDPNIEKTSEILLKLQEKGADLIELGIPYSDPLADGPIIQF
SASRALKSGTTSIKVIQLLESLKDKLRIPVILFTYFNPILNFGLENFCDLASKAGVAGLIIPDLPLEEASKFSEIISSYF
IDLILLVAPTTPFERMKMISNKTKGFTYLVSVTGVTGERSKMGNRVESLITKLKEISINPVAVGFGISAPEHVNRVSKWG
ADGVIIGSAFVKRISNSNEREVVNEVGNFCEEMRKAADQ

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=258, Percent_Identity=37.5968992248062, Blast_Score=162, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6321412, Length=245, Percent_Identity=40.8163265306122, Blast_Score=188, Evalue=1e-48,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 30815; Mature: 30815

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENKKPQFTKNETLSKINEKFLELKKSKKLALMPFIMAGDPNIEKTSEILLKLQEKGAD
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCC
LIELGIPYSDPLADGPIIQFSASRALKSGTTSIKVIQLLESLKDKLRIPVILFTYFNPIL
EEEECCCCCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NFGLENFCDLASKAGVAGLIIPDLPLEEASKFSEIISSYFIDLILLVAPTTPFERMKMIS
HHCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NKTKGFTYLVSVTGVTGERSKMGNRVESLITKLKEISINPVAVGFGISAPEHVNRVSKWG
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCC
ADGVIIGSAFVKRISNSNEREVVNEVGNFCEEMRKAADQ
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKENKKPQFTKNETLSKINEKFLELKKSKKLALMPFIMAGDPNIEKTSEILLKLQEKGAD
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCC
LIELGIPYSDPLADGPIIQFSASRALKSGTTSIKVIQLLESLKDKLRIPVILFTYFNPIL
EEEECCCCCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
NFGLENFCDLASKAGVAGLIIPDLPLEEASKFSEIISSYFIDLILLVAPTTPFERMKMIS
HHCHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
NKTKGFTYLVSVTGVTGERSKMGNRVESLITKLKEISINPVAVGFGISAPEHVNRVSKWG
CCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHCC
ADGVIIGSAFVKRISNSNEREVVNEVGNFCEEMRKAADQ
CCCEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]