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The map label for this gene is proS

Identifier: 123965807

GI number: 123965807

Start: 510506

End: 512308

Strand: Reverse

Name: proS

Synonym: P9515_05721

Alternate gene names: 123965807

Gene position: 512308-510506 (Counterclockwise)

Preceding gene: 123965813

Following gene: 123965806

Centisome position: 30.06

GC content: 31.61

Gene sequence:

>1803_bases
ATGCGCGTCACCACCTCCTTTCCTCTGGGGACGCTTCGTGATACTCCTTCTGAAGCAGAAATTATTTCTCATCAATTACT
TTTAAAAGGGGGTTATATTCGCAGAGTAAATAGTGGAATATATGCATACATGCCGATAATGCTCAAAGTAATTGAAAAAA
TATCGAACATAATTGAAAAAGAACTAAATAATAATGGCTGTTCAAAATTACTTTTACCCCAACTCCACCCAGCAGAGTTA
TGGAAAAGGAGTGAAAGATGGGAAGGTTATACAGCCGGGGAAGGAATAATGTTTAATCTCAAGGATAGGCAAGGGAAAGA
ATTTGGCTTGGCACCAACACATGAAGAAGTAATTACAAATATTGCATCAGAAATTATTAATTCATATAAGCAGCTACCTT
TATGTTTCTACCAAATACAAACAAAATTTAGAGATGAGATAAGGCCACGATTTGGGTTGATGAGGAGTAGGGAATTCATA
ATGAAGGATGGTTATTCTTTTCACTCTTCAAAAGAAGATTTGTCATCTTTTTATGAAAAAATGGAAAGATCATATGAAAA
TATTTTTAAAAATTGTGGATTAGATACAGTTGGAGTAGATGCAGATAGTGGAGCTATTGGAGGGGCCGCATCTAAAGAAT
TCATGGTAACAGCAGATGCCGGCGAAGATTATATTTTATTTACTGAAAGTGGTTCTTATGCAGCTAATATTGAAAAAGCC
ATTTCTTTACCTTCAAAAGAGATTCCTTTAAAAAGTTTTGAAACTGAATGGTTAGAAACACCTAATCAAAAATCAATTGT
TGATATATGTAAGGAAAATGATTTGGATGCTAGCCAAATAGTTAAAGTCGTTATATTCGTCGCTAAATTTGAAAACAAGT
CCCAACTTCCAATCCTCACATGTATAAGAGGAGATCAACATATAAATGAAATTAAACTTTTTAATTTGATTAGCAAAAAA
TATAGTTCAAACTTGATTAGCCTAGAAATAATTGAAGACAATGCAACTATTGAAAAAAATCTAACCAATTTTCCATTAGG
TTACATTGGACCAGACATTAATGATGAAGTAATAAAAAATAGTTCAAGTTGGGACAAAAGTTGGATAAGAATCGCTGATC
ACTCTGCCAATAATCTTTCAAGTTTTGTAAGTGGTAGCAATAAAGTTAATTTTCATAAAGTATTCCAAACATTTTCATTC
ATTGATAATCAATTCCTAATCTCAGATATAAGGAATGCAAAAAAAGGAGATCGTATTAGCCTTGAAAGTAATGAAGAGTT
AAAAGAAAAAAGAGGTATTGAAATTGGACATATTTTTCAATTAGGTCAAAAATATAGCGAGAAATTGAATGCAAAATTCT
CTGACAAGGATGGTAAATTGAAAAATTTATGGATGGGTTGTTATGGAATTGGCGTTACAAGAATCGCTCAAGCAGCTATT
GAACAGAATCATGATGAAAATGGTATTTCTTGGCCAATTCAAATTTCTCCCTTTGAGATTTTAATTATTCCAACTAATTT
AAAAGATCCATATCAAACAAAACTTACTGAAGAGATTTACAAGGAGTTTGAAAGCAAAAAAATTGATGTGTTACTTGACG
ACCGAGATGTTAGAGCAGGAGTTAAGTTCAAAGATGCGGATTTAATTGGTATTCCTTTTCAAATAATTATTGGCAGAGAT
TCGATCAATAAAGAAGTAGAATTTATATGCAGATCAAGCAAAAGAAAAATTAAGATTTCTTCACAGAATTTGTTAGAAAA
ATTTATTTCCGAATCAAAAATACTGTACAATGAAAATTCTTAA

Upstream 100 bases:

>100_bases
AAGGCTCAATCTAAACTACCTGCCTTCAGGGACTCAAGATACTTTAAATTTTTTTTACGACTTAATTGATAAAATTAAAC
ATAATTACAAATTTATTTAT

Downstream 100 bases:

>100_bases
AGCTAAATTTTATTCGGAGTAAAGTTATGTTAGTTAAAGAAAAGCCTTTATTATTTCCTGATTTTTTTGCAAAAGTTATA
TCTTTTACCATTTGTTTATC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKGGYIRRVNSGIYAYMPIMLKVIEKISNIIEKELNNNGCSKLLLPQLHPAEL
WKRSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITNIASEIINSYKQLPLCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSKEDLSSFYEKMERSYENIFKNCGLDTVGVDADSGAIGGAASKEFMVTADAGEDYILFTESGSYAANIEKA
ISLPSKEIPLKSFETEWLETPNQKSIVDICKENDLDASQIVKVVIFVAKFENKSQLPILTCIRGDQHINEIKLFNLISKK
YSSNLISLEIIEDNATIEKNLTNFPLGYIGPDINDEVIKNSSSWDKSWIRIADHSANNLSSFVSGSNKVNFHKVFQTFSF
IDNQFLISDIRNAKKGDRISLESNEELKEKRGIEIGHIFQLGQKYSEKLNAKFSDKDGKLKNLWMGCYGIGVTRIAQAAI
EQNHDENGISWPIQISPFEILIIPTNLKDPYQTKLTEEIYKEFESKKIDVLLDDRDVRAGVKFKDADLIGIPFQIIIGRD
SINKEVEFICRSSKRKIKISSQNLLEKFISESKILYNENS

Sequences:

>Translated_600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKGGYIRRVNSGIYAYMPIMLKVIEKISNIIEKELNNNGCSKLLLPQLHPAEL
WKRSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITNIASEIINSYKQLPLCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSKEDLSSFYEKMERSYENIFKNCGLDTVGVDADSGAIGGAASKEFMVTADAGEDYILFTESGSYAANIEKA
ISLPSKEIPLKSFETEWLETPNQKSIVDICKENDLDASQIVKVVIFVAKFENKSQLPILTCIRGDQHINEIKLFNLISKK
YSSNLISLEIIEDNATIEKNLTNFPLGYIGPDINDEVIKNSSSWDKSWIRIADHSANNLSSFVSGSNKVNFHKVFQTFSF
IDNQFLISDIRNAKKGDRISLESNEELKEKRGIEIGHIFQLGQKYSEKLNAKFSDKDGKLKNLWMGCYGIGVTRIAQAAI
EQNHDENGISWPIQISPFEILIIPTNLKDPYQTKLTEEIYKEFESKKIDVLLDDRDVRAGVKFKDADLIGIPFQIIIGRD
SINKEVEFICRSSKRKIKISSQNLLEKFISESKILYNENS
>Mature_600_residues
MRVTTSFPLGTLRDTPSEAEIISHQLLLKGGYIRRVNSGIYAYMPIMLKVIEKISNIIEKELNNNGCSKLLLPQLHPAEL
WKRSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITNIASEIINSYKQLPLCFYQIQTKFRDEIRPRFGLMRSREFI
MKDGYSFHSSKEDLSSFYEKMERSYENIFKNCGLDTVGVDADSGAIGGAASKEFMVTADAGEDYILFTESGSYAANIEKA
ISLPSKEIPLKSFETEWLETPNQKSIVDICKENDLDASQIVKVVIFVAKFENKSQLPILTCIRGDQHINEIKLFNLISKK
YSSNLISLEIIEDNATIEKNLTNFPLGYIGPDINDEVIKNSSSWDKSWIRIADHSANNLSSFVSGSNKVNFHKVFQTFSF
IDNQFLISDIRNAKKGDRISLESNEELKEKRGIEIGHIFQLGQKYSEKLNAKFSDKDGKLKNLWMGCYGIGVTRIAQAAI
EQNHDENGISWPIQISPFEILIIPTNLKDPYQTKLTEEIYKEFESKKIDVLLDDRDVRAGVKFKDADLIGIPFQIIIGRD
SINKEVEFICRSSKRKIKISSQNLLEKFISESKILYNENS

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=245, Percent_Identity=41.2244897959184, Blast_Score=188, Evalue=1e-47,
Organism=Escherichia coli, GI1786392, Length=593, Percent_Identity=41.3153456998314, Blast_Score=452, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI115532348, Length=273, Percent_Identity=33.3333333333333, Blast_Score=147, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6320931, Length=226, Percent_Identity=36.283185840708, Blast_Score=165, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24656200, Length=227, Percent_Identity=36.5638766519824, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_PROM5 (A2BVH0)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010888.1
- ProteinModelPortal:   A2BVH0
- SMR:   A2BVH0
- STRING:   A2BVH0
- GeneID:   4719706
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_05721
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- ProtClustDB:   PRK09194
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 68348; Mature: 68348

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTTSFPLGTLRDTPSEAEIISHQLLLKGGYIRRVNSGIYAYMPIMLKVIEKISNIIEK
CEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
ELNNNGCSKLLLPQLHPAELWKRSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITN
HHCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHH
IASEIINSYKQLPLCFYQIQTKFRDEIRPRFGLMRSREFIMKDGYSFHSSKEDLSSFYEK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
MERSYENIFKNCGLDTVGVDADSGAIGGAASKEFMVTADAGEDYILFTESGSYAANIEKA
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHH
ISLPSKEIPLKSFETEWLETPNQKSIVDICKENDLDASQIVKVVIFVAKFENKSQLPILT
HCCCCCCCCCHHCCHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
CIRGDQHINEIKLFNLISKKYSSNLISLEIIEDNATIEKNLTNFPLGYIGPDINDEVIKN
EECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEECCCCCCCCCCCCCCCCCHHHHCC
SSSWDKSWIRIADHSANNLSSFVSGSNKVNFHKVFQTFSFIDNQFLISDIRNAKKGDRIS
CCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEE
LESNEELKEKRGIEIGHIFQLGQKYSEKLNAKFSDKDGKLKNLWMGCYGIGVTRIAQAAI
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQNHDENGISWPIQISPFEILIIPTNLKDPYQTKLTEEIYKEFESKKIDVLLDDRDVRAG
HCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
VKFKDADLIGIPFQIIIGRDSINKEVEFICRSSKRKIKISSQNLLEKFISESKILYNENS
CEECCCCEECCCEEEEECCCCCCHHHHHHHCCCCCEEEECHHHHHHHHHCCCEEEEECCC
>Mature Secondary Structure
MRVTTSFPLGTLRDTPSEAEIISHQLLLKGGYIRRVNSGIYAYMPIMLKVIEKISNIIEK
CEEECCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHH
ELNNNGCSKLLLPQLHPAELWKRSERWEGYTAGEGIMFNLKDRQGKEFGLAPTHEEVITN
HHCCCCCCCCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECCCCCCCCCCCCCHHHHHHH
IASEIINSYKQLPLCFYQIQTKFRDEIRPRFGLMRSREFIMKDGYSFHSSKEDLSSFYEK
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
MERSYENIFKNCGLDTVGVDADSGAIGGAASKEFMVTADAGEDYILFTESGSYAANIEKA
HHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCHHHHHHH
ISLPSKEIPLKSFETEWLETPNQKSIVDICKENDLDASQIVKVVIFVAKFENKSQLPILT
HCCCCCCCCCHHCCHHHHCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
CIRGDQHINEIKLFNLISKKYSSNLISLEIIEDNATIEKNLTNFPLGYIGPDINDEVIKN
EECCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCEECCCCCCCCCCCCCCCCCHHHHCC
SSSWDKSWIRIADHSANNLSSFVSGSNKVNFHKVFQTFSFIDNQFLISDIRNAKKGDRIS
CCCCCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCEEE
LESNEELKEKRGIEIGHIFQLGQKYSEKLNAKFSDKDGKLKNLWMGCYGIGVTRIAQAAI
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EQNHDENGISWPIQISPFEILIIPTNLKDPYQTKLTEEIYKEFESKKIDVLLDDRDVRAG
HCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
VKFKDADLIGIPFQIIIGRDSINKEVEFICRSSKRKIKISSQNLLEKFISESKILYNENS
CEECCCCEECCCEEEEECCCCCCHHHHHHHCCCCCEEEECHHHHHHHHHCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA