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The map label for this gene is priA [H]

Identifier: 123965796

GI number: 123965796

Start: 500978

End: 503251

Strand: Direct

Name: priA [H]

Synonym: P9515_05611

Alternate gene names: 123965796

Gene position: 500978-503251 (Clockwise)

Preceding gene: 123965793

Following gene: 123965800

Centisome position: 29.4

GC content: 32.54

Gene sequence:

>2274_bases
ATGACGGCAAGTAATAATTTATCAAATACCTCTTTTAAGTTTGAGGTCTTGCTTGATATAGGTAGTCATAGTAATAGTTT
CTCTTATTTAGACGGTAATAATCTCGGAGTAGAAGTTGGTGATATTGTTTCAGTAAAACTTAAAGGAAGATTATTAAATG
GATTAACGATTGCTAGAAACCCTTTCTTAAAAAGGGATAAAAATGGAAAAGATTTTGATGAAGAATCTAGGTTTAGATAT
TTGTCTATAGAAGGTATTGTGCAAAAAAAAGTGATTCAAGACTGGTGGAGAGAATGGCTGGAGACTTTAGCTTTATCTTA
TCGAGTAAGCAACTTAAAAATGTTTAAAACAGCTTTTCCTCCGGGATGGATTGGAAAACATAAAAAAATATCTCAAAAAT
ATAGATATCAAATATGGATTAAATTACAACCTAATTTTCACTTCAGGAATTATGAATTAAATAAGAGAGAACTTTCATTA
ATAAATTTTCTAGGTCTTAAAGGGAAATGGCAAAGCGAATTAATTAAGTTTGGTTTTAATTCAAAACTTATAAATTTAAT
GATTAGTAAAAAACTTTTGATAAAGACTAAACGAAAAAAAATATTTAATACCAAATTAAGTTCTTTTAAAAATGATTGTA
TTTCAATTAAAAAACCAAATCTTACACAGGAGCAAAAAAAAGTATATGTGGAAATGCAAGAGATGCAACCTGGAGAGGTC
TGTTTGCTCTGGGGAGAAACAGGTTCTGGCAAAACAGAAGTTTATATGAGAATGGCTGAAGATCAACTACTTAATAAAAA
GAGCTGTTTAATATTGGCTCCGGAAATTGGCTTGATTCCTCAACTTATTGATAGATTTAGTAAAAGATTTCAAAGTGAGG
TCATTGAGTATCACAGTAATTGTTCTTCAAGGCATAGGACTTTAGTTTGGGAAAAAATAATAGATGAAGATGAACCCGTT
ATTGTTGTAGGGACAAGATCTGCAGTATTCCTTCCTATTCGGAATTTAGGCTTAATAGTGATGGATGAAGAACATGATGT
TTCCTATAAGCAAGATAGTCCAATGCCTTGTTACGATGCGAGAGATGTTGCCCTTGAAAGAGTAAAAAGAAATCCAACTA
AGCTAATTTTTGGAAGTGCAACTCCTTCAATGAGAACTTGGAAAAGGGTTGTTTATGAAAAAAAAATAAGGTTAATAAGA
ATGAAGGAAAGAATATCTACTACAGAAATACCCGAAATTAAAGTTGTTGATATGCGTAGTGAATTTAAAAAGGGAAATAC
AAAAATTTTTTGCAGCGAATTATTAGAATTAATTTCTAAGCTTAAAGAGAATCAAGAACAAGCAATAATTTTGATTCCTA
GAAGAGGTTATAACGGTTTTCTAAGTTGTAGAAATTGTGGATTTATAATCAATTGCCCTAATTGTGATGTACCTTTATCC
GTTCATATGGGATCAAAAGGTAAACAGTGGCTTAGCTGTCACTGGTGTGACCATAAAGCAAAACTTATTTACTCTTGTCC
AGATTGTAAATCAAATGCCTTTAAGCCTTTTGGGATAGGAACTCAAAGAGTTATTGAGTTCTTGAATAATGAATTCCCCG
AATTGAGGGTACTTCGTTTTGATAGAGACACTACCTCAGGTAAAGATGGTCATAGAAATATCCTTTCAATGTTTTCTAAT
GGGAATGCGGATATTCTTGTAGGAACTCAAATGTTAGCAAAAGGGATTGATATTCCCAATGTTACTCTTTCTGTGGTTAT
TGCTGCAGATGGACTTCTTCATCGTCCAGACATTTCTTCAGAAGAAAAATCATTACAATTATTTCTACAATTAGCAGGCA
GAGCAGGTAGAGCTGATAAAACAGGAAAGGTTGTTTTTCAAACATATAAACCAAGTCATCCTGTCCTCTGTTATCTCAAA
AATAGAAATTATGAGGGTTTTTTAGCTGAAAGCTCTATCTTAAGAAAAGATGCAAAACTATTTCCTTTTTGCAATGTATG
CCTTTTTAAAGTTTCTGGAAAGAACTATGAATGTACTGAAAATACTGCAGCTGAGCTGGCAAAATATTTGATGAGTTTTT
GTCAAGGAGATAAGTGGACGGTAATTGGTCCTGCTCCAAGTTTAATTGCAAAAGTTGGGAATAAATTTAGGTGGCAAATA
TTAATGCATGGTCCAGAAAACTCGGAAGTCCCTTTGCCAGATAGGGCTAAATTATGGCAATTAATTCCCAAAAATGTCTT
TTTATCACTTGATCTGAATCCGGTAGAGCTATAG

Upstream 100 bases:

>100_bases
AATGATCAAAGCTTCATGTCTCCCTTAAGAACAGGATACTTACAATGTGCAAAAACACTTTGTGGAATGTTCAACAATCC
AATACTAAGAAAAAGTTTTG

Downstream 100 bases:

>100_bases
CTAATTGGAAGGAAGTTCTGGAAAGTTAGAACCTATCTGGTATCTATAAAATCTTATTAAATAAGTAAGTAATATCGATA
CTAAGATAAGTAAAAACCCT

Product: primosomal protein N' (replication factor Y)

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 757; Mature: 756

Protein sequence:

>757_residues
MTASNNLSNTSFKFEVLLDIGSHSNSFSYLDGNNLGVEVGDIVSVKLKGRLLNGLTIARNPFLKRDKNGKDFDEESRFRY
LSIEGIVQKKVIQDWWREWLETLALSYRVSNLKMFKTAFPPGWIGKHKKISQKYRYQIWIKLQPNFHFRNYELNKRELSL
INFLGLKGKWQSELIKFGFNSKLINLMISKKLLIKTKRKKIFNTKLSSFKNDCISIKKPNLTQEQKKVYVEMQEMQPGEV
CLLWGETGSGKTEVYMRMAEDQLLNKKSCLILAPEIGLIPQLIDRFSKRFQSEVIEYHSNCSSRHRTLVWEKIIDEDEPV
IVVGTRSAVFLPIRNLGLIVMDEEHDVSYKQDSPMPCYDARDVALERVKRNPTKLIFGSATPSMRTWKRVVYEKKIRLIR
MKERISTTEIPEIKVVDMRSEFKKGNTKIFCSELLELISKLKENQEQAIILIPRRGYNGFLSCRNCGFIINCPNCDVPLS
VHMGSKGKQWLSCHWCDHKAKLIYSCPDCKSNAFKPFGIGTQRVIEFLNNEFPELRVLRFDRDTTSGKDGHRNILSMFSN
GNADILVGTQMLAKGIDIPNVTLSVVIAADGLLHRPDISSEEKSLQLFLQLAGRAGRADKTGKVVFQTYKPSHPVLCYLK
NRNYEGFLAESSILRKDAKLFPFCNVCLFKVSGKNYECTENTAAELAKYLMSFCQGDKWTVIGPAPSLIAKVGNKFRWQI
LMHGPENSEVPLPDRAKLWQLIPKNVFLSLDLNPVEL

Sequences:

>Translated_757_residues
MTASNNLSNTSFKFEVLLDIGSHSNSFSYLDGNNLGVEVGDIVSVKLKGRLLNGLTIARNPFLKRDKNGKDFDEESRFRY
LSIEGIVQKKVIQDWWREWLETLALSYRVSNLKMFKTAFPPGWIGKHKKISQKYRYQIWIKLQPNFHFRNYELNKRELSL
INFLGLKGKWQSELIKFGFNSKLINLMISKKLLIKTKRKKIFNTKLSSFKNDCISIKKPNLTQEQKKVYVEMQEMQPGEV
CLLWGETGSGKTEVYMRMAEDQLLNKKSCLILAPEIGLIPQLIDRFSKRFQSEVIEYHSNCSSRHRTLVWEKIIDEDEPV
IVVGTRSAVFLPIRNLGLIVMDEEHDVSYKQDSPMPCYDARDVALERVKRNPTKLIFGSATPSMRTWKRVVYEKKIRLIR
MKERISTTEIPEIKVVDMRSEFKKGNTKIFCSELLELISKLKENQEQAIILIPRRGYNGFLSCRNCGFIINCPNCDVPLS
VHMGSKGKQWLSCHWCDHKAKLIYSCPDCKSNAFKPFGIGTQRVIEFLNNEFPELRVLRFDRDTTSGKDGHRNILSMFSN
GNADILVGTQMLAKGIDIPNVTLSVVIAADGLLHRPDISSEEKSLQLFLQLAGRAGRADKTGKVVFQTYKPSHPVLCYLK
NRNYEGFLAESSILRKDAKLFPFCNVCLFKVSGKNYECTENTAAELAKYLMSFCQGDKWTVIGPAPSLIAKVGNKFRWQI
LMHGPENSEVPLPDRAKLWQLIPKNVFLSLDLNPVEL
>Mature_756_residues
TASNNLSNTSFKFEVLLDIGSHSNSFSYLDGNNLGVEVGDIVSVKLKGRLLNGLTIARNPFLKRDKNGKDFDEESRFRYL
SIEGIVQKKVIQDWWREWLETLALSYRVSNLKMFKTAFPPGWIGKHKKISQKYRYQIWIKLQPNFHFRNYELNKRELSLI
NFLGLKGKWQSELIKFGFNSKLINLMISKKLLIKTKRKKIFNTKLSSFKNDCISIKKPNLTQEQKKVYVEMQEMQPGEVC
LLWGETGSGKTEVYMRMAEDQLLNKKSCLILAPEIGLIPQLIDRFSKRFQSEVIEYHSNCSSRHRTLVWEKIIDEDEPVI
VVGTRSAVFLPIRNLGLIVMDEEHDVSYKQDSPMPCYDARDVALERVKRNPTKLIFGSATPSMRTWKRVVYEKKIRLIRM
KERISTTEIPEIKVVDMRSEFKKGNTKIFCSELLELISKLKENQEQAIILIPRRGYNGFLSCRNCGFIINCPNCDVPLSV
HMGSKGKQWLSCHWCDHKAKLIYSCPDCKSNAFKPFGIGTQRVIEFLNNEFPELRVLRFDRDTTSGKDGHRNILSMFSNG
NADILVGTQMLAKGIDIPNVTLSVVIAADGLLHRPDISSEEKSLQLFLQLAGRAGRADKTGKVVFQTYKPSHPVLCYLKN
RNYEGFLAESSILRKDAKLFPFCNVCLFKVSGKNYECTENTAAELAKYLMSFCQGDKWTVIGPAPSLIAKVGNKFRWQIL
MHGPENSEVPLPDRAKLWQLIPKNVFLSLDLNPVEL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=600, Percent_Identity=34.8333333333333, Blast_Score=336, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 86906; Mature: 86775

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASNNLSNTSFKFEVLLDIGSHSNSFSYLDGNNLGVEVGDIVSVKLKGRLLNGLTIARN
CCCCCCCCCCEEEEEEEEEECCCCCCEEEECCCCCCEEECCEEEEEEECEEECCEEEECC
PFLKRDKNGKDFDEESRFRYLSIEGIVQKKVIQDWWREWLETLALSYRVSNLKMFKTAFP
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
PGWIGKHKKISQKYRYQIWIKLQPNFHFRNYELNKRELSLINFLGLKGKWQSELIKFGFN
CCCCCCCHHHCCCEEEEEEEEECCCEEEECEECCHHHHHHHHHHCCCCCCHHHHHHHCCC
SKLINLMISKKLLIKTKRKKIFNTKLSSFKNDCISIKKPNLTQEQKKVYVEMQEMQPGEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHEEEEHHHCCCCCE
CLLWGETGSGKTEVYMRMAEDQLLNKKSCLILAPEIGLIPQLIDRFSKRFQSEVIEYHSN
EEEECCCCCCCCCEEEEEHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
CSSRHRTLVWEKIIDEDEPVIVVGTRSAVFLPIRNLGLIVMDEEHDVSYKQDSPMPCYDA
CCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCH
RDVALERVKRNPTKLIFGSATPSMRTWKRVVYEKKIRLIRMKERISTTEIPEIKVVDMRS
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHH
EFKKGNTKIFCSELLELISKLKENQEQAIILIPRRGYNGFLSCRNCGFIINCPNCDVPLS
HHCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCEEEEECCCCCCEEE
VHMGSKGKQWLSCHWCDHKAKLIYSCPDCKSNAFKPFGIGTQRVIEFLNNEFPELRVLRF
EEECCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEE
DRDTTSGKDGHRNILSMFSNGNADILVGTQMLAKGIDIPNVTLSVVIAADGLLHRPDISS
CCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCEEEEEEEECCCEECCCCCC
EEKSLQLFLQLAGRAGRADKTGKVVFQTYKPSHPVLCYLKNRNYEGFLAESSILRKDAKL
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHCCCCC
FPFCNVCLFKVSGKNYECTENTAAELAKYLMSFCQGDKWTVIGPAPSLIAKVGNKFRWQI
CCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEE
LMHGPENSEVPLPDRAKLWQLIPKNVFLSLDLNPVEL
EEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure 
TASNNLSNTSFKFEVLLDIGSHSNSFSYLDGNNLGVEVGDIVSVKLKGRLLNGLTIARN
CCCCCCCCCEEEEEEEEEECCCCCCEEEECCCCCCEEECCEEEEEEECEEECCEEEECC
PFLKRDKNGKDFDEESRFRYLSIEGIVQKKVIQDWWREWLETLALSYRVSNLKMFKTAFP
CCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCC
PGWIGKHKKISQKYRYQIWIKLQPNFHFRNYELNKRELSLINFLGLKGKWQSELIKFGFN
CCCCCCCHHHCCCEEEEEEEEECCCEEEECEECCHHHHHHHHHHCCCCCCHHHHHHHCCC
SKLINLMISKKLLIKTKRKKIFNTKLSSFKNDCISIKKPNLTQEQKKVYVEMQEMQPGEV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHEEEEHHHCCCCCE
CLLWGETGSGKTEVYMRMAEDQLLNKKSCLILAPEIGLIPQLIDRFSKRFQSEVIEYHSN
EEEECCCCCCCCCEEEEEHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHC
CSSRHRTLVWEKIIDEDEPVIVVGTRSAVFLPIRNLGLIVMDEEHDVSYKQDSPMPCYDA
CCHHHHHHHHHHHCCCCCCEEEEECCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCH
RDVALERVKRNPTKLIFGSATPSMRTWKRVVYEKKIRLIRMKERISTTEIPEIKVVDMRS
HHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHH
EFKKGNTKIFCSELLELISKLKENQEQAIILIPRRGYNGFLSCRNCGFIINCPNCDVPLS
HHCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCEEEEECCCCCCEEE
VHMGSKGKQWLSCHWCDHKAKLIYSCPDCKSNAFKPFGIGTQRVIEFLNNEFPELRVLRF
EEECCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEE
DRDTTSGKDGHRNILSMFSNGNADILVGTQMLAKGIDIPNVTLSVVIAADGLLHRPDISS
CCCCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCEEEEEEEECCCEECCCCCC
EEKSLQLFLQLAGRAGRADKTGKVVFQTYKPSHPVLCYLKNRNYEGFLAESSILRKDAKL
HHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCEEEEEECCCCCCEEHHHHHHHCCCCC
FPFCNVCLFKVSGKNYECTENTAAELAKYLMSFCQGDKWTVIGPAPSLIAKVGNKFRWQI
CCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHCCCCEEEEE
LMHGPENSEVPLPDRAKLWQLIPKNVFLSLDLNPVEL
EEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]