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The map label for this gene is hemC

Identifier: 123965794

GI number: 123965794

Start: 498406

End: 499356

Strand: Reverse

Name: hemC

Synonym: P9515_05591

Alternate gene names: 123965794

Gene position: 499356-498406 (Counterclockwise)

Preceding gene: 123965795

Following gene: 123965791

Centisome position: 29.3

GC content: 33.65

Gene sequence:

>951_bases
ATGACTAAATTAAAATTAAAGATAGCTAGTAGAAGAAGTAAATTAGCAATGGTTCAAACATTATGGGTTAAAGAGCAATT
AGAAAAAAATATTCCTAACTTGGAAGTATCCATAGAGTCCATGGCAACACAAGGGGATAAAATTCTCGATGTGGCATTAG
CAAAAATAGGAGATAAAGGACTTTTTACAAAAGAACTTGAAGCTCAAATGCTCATTGGTAAAGCGGACATTGCGGTGCAT
TCTTTAAAAGATTTGCCAACCAACTTACCTGACGGACTAAAATTAGGATGTATAACAAAAAGAGAAGATCCTTCTGATGC
TCTAGTAGTTAATAAAAAAAATGAAATTTATCAATTAGAGAGTCTTCCCGCGGGTTCAATTGTTGGAACTAGTTCTCTTA
GGAGACTGGCTCAATTAAGGTACAAATTCCCATATCTTAATTTCAAAGACATCAGGGGAAATGTTATTACAAGAATAGAA
AAGTTAGATTCCGGAGAATTTGATTGTATTATTCTTGCTGCAGCGGGTTTAAAAAGATTGGGATTTGAATCAAGAATACA
CCAAATTATTCCTAACGAGATTTCCCTCCATGCAGTTGGGCAAGGGGCTTTAGGAATAGAATGTAAATCTGAGGATAAAG
AAGTTCTAAAAATTATAAGTGTCTTAGAAGATAAGGCCTCAAGCCAAAAATGTTTAGCAGAGAGATCTTTTTTAAGAGAA
CTTGAAGGTGGATGTCAAGTTCCAATTGGAGTTAATAGTAGTATTCAAAATGATGAAATATGCCTAAAAGGAATGGTCGC
ATCTATTGATGGGAAAAAACTTATTAAGGATGAATCAAGTGGAAATGTTAAATATCCAGAGGAAGTTGGTAAAAAACTAG
CTGAAAAGCTGAAACTTCAAGGAGCAGATAAAATTCTCTCGGAAATATTTGAACAATTTAGGGATAAATAA

Upstream 100 bases:

>100_bases
AATAAGAAAGTTAAATATAATTTTCAGCTTTGTAAAGTTGCCAAAAATTATCTTAAGATAAATTCGACTTTATCTTAAAC
CTATTTAAACTTATCATTTA

Downstream 100 bases:

>100_bases
TCTTTTATTAATCAACCAATTTAGGTTCAATTTCTTTGTGATATCTAGATTCACAATCTTGAATTACTTTTACAGCTTCC
TCAATTCCATAAAATCCATT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MTKLKLKIASRRSKLAMVQTLWVKEQLEKNIPNLEVSIESMATQGDKILDVALAKIGDKGLFTKELEAQMLIGKADIAVH
SLKDLPTNLPDGLKLGCITKREDPSDALVVNKKNEIYQLESLPAGSIVGTSSLRRLAQLRYKFPYLNFKDIRGNVITRIE
KLDSGEFDCIILAAAGLKRLGFESRIHQIIPNEISLHAVGQGALGIECKSEDKEVLKIISVLEDKASSQKCLAERSFLRE
LEGGCQVPIGVNSSIQNDEICLKGMVASIDGKKLIKDESSGNVKYPEEVGKKLAEKLKLQGADKILSEIFEQFRDK

Sequences:

>Translated_316_residues
MTKLKLKIASRRSKLAMVQTLWVKEQLEKNIPNLEVSIESMATQGDKILDVALAKIGDKGLFTKELEAQMLIGKADIAVH
SLKDLPTNLPDGLKLGCITKREDPSDALVVNKKNEIYQLESLPAGSIVGTSSLRRLAQLRYKFPYLNFKDIRGNVITRIE
KLDSGEFDCIILAAAGLKRLGFESRIHQIIPNEISLHAVGQGALGIECKSEDKEVLKIISVLEDKASSQKCLAERSFLRE
LEGGCQVPIGVNSSIQNDEICLKGMVASIDGKKLIKDESSGNVKYPEEVGKKLAEKLKLQGADKILSEIFEQFRDK
>Mature_315_residues
TKLKLKIASRRSKLAMVQTLWVKEQLEKNIPNLEVSIESMATQGDKILDVALAKIGDKGLFTKELEAQMLIGKADIAVHS
LKDLPTNLPDGLKLGCITKREDPSDALVVNKKNEIYQLESLPAGSIVGTSSLRRLAQLRYKFPYLNFKDIRGNVITRIEK
LDSGEFDCIILAAAGLKRLGFESRIHQIIPNEISLHAVGQGALGIECKSEDKEVLKIISVLEDKASSQKCLAERSFLREL
EGGCQVPIGVNSSIQNDEICLKGMVASIDGKKLIKDESSGNVKYPEEVGKKLAEKLKLQGADKILSEIFEQFRDK

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=284, Percent_Identity=48.2394366197183, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI66933009, Length=284, Percent_Identity=48.2394366197183, Blast_Score=280, Evalue=9e-76,
Organism=Escherichia coli, GI48994974, Length=305, Percent_Identity=50.1639344262295, Blast_Score=291, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6319996, Length=316, Percent_Identity=43.9873417721519, Blast_Score=246, Evalue=3e-66,
Organism=Drosophila melanogaster, GI20130425, Length=284, Percent_Identity=44.3661971830986, Blast_Score=239, Evalue=2e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_PROM5 (A2BVF7)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010875.1
- ProteinModelPortal:   A2BVF7
- SMR:   A2BVF7
- STRING:   A2BVF7
- GeneID:   4719777
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_05591
- eggNOG:   COG0181
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   PMAR167542:P9515ORF_0595-MONOMER
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34915; Mature: 34784

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKLKLKIASRRSKLAMVQTLWVKEQLEKNIPNLEVSIESMATQGDKILDVALAKIGDKG
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHHHHHHCCCC
LFTKELEAQMLIGKADIAVHSLKDLPTNLPDGLKLGCITKREDPSDALVVNKKNEIYQLE
CCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEC
SLPAGSIVGTSSLRRLAQLRYKFPYLNFKDIRGNVITRIEKLDSGEFDCIILAAAGLKRL
CCCCCCEECHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCCEEEEECHHHHHC
GFESRIHQIIPNEISLHAVGQGALGIECKSEDKEVLKIISVLEDKASSQKCLAERSFLRE
CHHHHHHHHCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LEGGCQVPIGVNSSIQNDEICLKGMVASIDGKKLIKDESSGNVKYPEEVGKKLAEKLKLQ
HCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCEECHHHHHHHHHHHHHCC
GADKILSEIFEQFRDK
CHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKLKLKIASRRSKLAMVQTLWVKEQLEKNIPNLEVSIESMATQGDKILDVALAKIGDKG
CEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHCCCCHHHHHHHHHHCCCC
LFTKELEAQMLIGKADIAVHSLKDLPTNLPDGLKLGCITKREDPSDALVVNKKNEIYQLE
CCHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEEC
SLPAGSIVGTSSLRRLAQLRYKFPYLNFKDIRGNVITRIEKLDSGEFDCIILAAAGLKRL
CCCCCCEECHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHCCCCCCCEEEEECHHHHHC
GFESRIHQIIPNEISLHAVGQGALGIECKSEDKEVLKIISVLEDKASSQKCLAERSFLRE
CHHHHHHHHCCCCEEEEEECCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LEGGCQVPIGVNSSIQNDEICLKGMVASIDGKKLIKDESSGNVKYPEEVGKKLAEKLKLQ
HCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCEECHHHHHHHHHHHHHCC
GADKILSEIFEQFRDK
CHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA