Click here to switch to the map view.

The map label for this gene is gltX [H]

Identifier: 123965772

GI number: 123965772

Start: 479032

End: 480513

Strand: Reverse

Name: gltX [H]

Synonym: P9515_05371

Alternate gene names: 123965772

Gene position: 480513-479032 (Counterclockwise)

Preceding gene: 123965773

Following gene: 123965768

Centisome position: 28.2

GC content: 31.71

Gene sequence:

>1482_bases
TTGCGGGTTCGAATCCCGTCGGTCCCGTTCTTTTCTAAAAAGAAATTGGAAAAACGTTTAAGGTTAGCGCCCAGTCCAAC
TGGTTTATTACACATAGGTACAGCAAGAACAGCACTATTTAATTGGTTATATGCAAAAAAAACTAATGGAAAATTCTTTA
TTCGAATAGAAGATACTGATATTGTCCGATCTAAGTCTGAATATACAACCAACATATTAGATGGATTAAATTGGTTAGGA
CTTTCTTGGGATGAAGAGCCTATTAAACAAAGCAAACGAATTTCTATTTACAAAAAAAATATTAAAAAACTTTTGGAAAT
TGGAGCTGCATATAGATGCTTTACTTCAGAAAGTGAGATTTCTGAGCTTAGAGAAAAGCAAAAATCAAGTGGGTTGCCAC
CAAGGCATGACAATAGACATAGAAATCTTACGAGTAACGAAATTAAAGCATTTTTATCTCAAGGAAGATCTTCAGTCATA
AGGTTTAAGATTGATGATGAAACAGAAATCAAATGGGAAGATCAGATTAGAGGCGAAATAAAATGGAAAGGGAGGGATCT
TGGGGGCGATTTGGTTTTGTCTAGAAGAGCTTTAGGCGATGAAATAGGAGATCCACTATATAATCTTGCTGTCGTGGTAG
ATGACAATTTCATGAATATTACTCATGTTGTAAGGGGTGAAGATCACATTTCAAATACTGCAAAACAAATCTTGATTTAT
AAGGCCTTAAATTTTAAATTGCCAATTTTTTCTCATACTCCTCTTATATTGAATAGTGAAGGTAAAAAGCTCTCAAAACG
TGATTGCGTAACTTCAATTGATGAATTCAGAGAAATGGGATATTTACCAGAAGCTTTAGCCAATTACATGGCATTTTTGG
GATGGTCAATAAAAGATTCTGAAAGTGAAATTCTCTCATTAAAAGAGATATCTAGAGTTTTTAATTTATCAGATATAAAT
AAAGCTGGTGCAAAATTTAATTGGGAAAAACTTAATTGGATTAATTCTCAATATATTAAGCAAATGGAATTAACAAAATT
AAGACAACTCATAAAAAATTACTGGGATGATATGGGTTGGGAATCTCCTTCAGCAGAATGGGATTTGAAACTAATAAATC
TAATTCGAGACTCAATGCTTTTGTTAAAGGATGCAATTGATCAATCAAAACCATTTTTTACTTTGTCTCAAATGCAAAAA
GAGGGGGAAGAATTTCTGAACAATAAAGATGAGACAAGAGAGTCTTTAAAATATATCCTTTGCTATCTAAAAGAAGAAAA
TATATCAACCATTGATAGTAATAAAGCTAAAGAAATAATTCACAAAATTTCTGTTGAAAACTCTATAAAAAAAGGGATTT
TAATGAAGTCATTGAGGGTAGCTTTTTTTGGTTGTCTTAGCGGACCAGATTTAATTCAAAGTTGGGAATTATTCTCTGAA
AACAAAAGTGATATAACCTTAATTGAAAGATGTCTTATTTAA

Upstream 100 bases:

>100_bases
GTATTATTATCTGATAATTTTAAAGTCAGTTCCTTAATTAGAACAAAAATTCTGGGACCGTAGTTCAACTGGTTAGAGCA
CCGCCCTGTCACGGCGGAAG

Downstream 100 bases:

>100_bases
CTATATTTTCTTTTCTATTAAATCCAGAAAATTCGCGAGAGGTCTTGCTGTAAGACCCTGAATCCCAACTGTCATGAGTA
TCGTAAGAAATACCAAACCT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MRVRIPSVPFFSKKKLEKRLRLAPSPTGLLHIGTARTALFNWLYAKKTNGKFFIRIEDTDIVRSKSEYTTNILDGLNWLG
LSWDEEPIKQSKRISIYKKNIKKLLEIGAAYRCFTSESEISELREKQKSSGLPPRHDNRHRNLTSNEIKAFLSQGRSSVI
RFKIDDETEIKWEDQIRGEIKWKGRDLGGDLVLSRRALGDEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIY
KALNFKLPIFSHTPLILNSEGKKLSKRDCVTSIDEFREMGYLPEALANYMAFLGWSIKDSESEILSLKEISRVFNLSDIN
KAGAKFNWEKLNWINSQYIKQMELTKLRQLIKNYWDDMGWESPSAEWDLKLINLIRDSMLLLKDAIDQSKPFFTLSQMQK
EGEEFLNNKDETRESLKYILCYLKEENISTIDSNKAKEIIHKISVENSIKKGILMKSLRVAFFGCLSGPDLIQSWELFSE
NKSDITLIERCLI

Sequences:

>Translated_493_residues
MRVRIPSVPFFSKKKLEKRLRLAPSPTGLLHIGTARTALFNWLYAKKTNGKFFIRIEDTDIVRSKSEYTTNILDGLNWLG
LSWDEEPIKQSKRISIYKKNIKKLLEIGAAYRCFTSESEISELREKQKSSGLPPRHDNRHRNLTSNEIKAFLSQGRSSVI
RFKIDDETEIKWEDQIRGEIKWKGRDLGGDLVLSRRALGDEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIY
KALNFKLPIFSHTPLILNSEGKKLSKRDCVTSIDEFREMGYLPEALANYMAFLGWSIKDSESEILSLKEISRVFNLSDIN
KAGAKFNWEKLNWINSQYIKQMELTKLRQLIKNYWDDMGWESPSAEWDLKLINLIRDSMLLLKDAIDQSKPFFTLSQMQK
EGEEFLNNKDETRESLKYILCYLKEENISTIDSNKAKEIIHKISVENSIKKGILMKSLRVAFFGCLSGPDLIQSWELFSE
NKSDITLIERCLI
>Mature_493_residues
MRVRIPSVPFFSKKKLEKRLRLAPSPTGLLHIGTARTALFNWLYAKKTNGKFFIRIEDTDIVRSKSEYTTNILDGLNWLG
LSWDEEPIKQSKRISIYKKNIKKLLEIGAAYRCFTSESEISELREKQKSSGLPPRHDNRHRNLTSNEIKAFLSQGRSSVI
RFKIDDETEIKWEDQIRGEIKWKGRDLGGDLVLSRRALGDEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIY
KALNFKLPIFSHTPLILNSEGKKLSKRDCVTSIDEFREMGYLPEALANYMAFLGWSIKDSESEILSLKEISRVFNLSDIN
KAGAKFNWEKLNWINSQYIKQMELTKLRQLIKNYWDDMGWESPSAEWDLKLINLIRDSMLLLKDAIDQSKPFFTLSQMQK
EGEEFLNNKDETRESLKYILCYLKEENISTIDSNKAKEIIHKISVENSIKKGILMKSLRVAFFGCLSGPDLIQSWELFSE
NKSDITLIERCLI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=343, Percent_Identity=34.9854227405248, Blast_Score=217, Evalue=2e-56,
Organism=Escherichia coli, GI1788743, Length=460, Percent_Identity=36.7391304347826, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI226510925, Length=268, Percent_Identity=32.8358208955224, Blast_Score=131, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71993394, Length=474, Percent_Identity=32.0675105485232, Blast_Score=202, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324540, Length=503, Percent_Identity=32.8031809145129, Blast_Score=239, Evalue=1e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=226, Percent_Identity=25.2212389380531, Blast_Score=71, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24665321, Length=483, Percent_Identity=34.3685300207039, Blast_Score=253, Evalue=3e-67,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 57106; Mature: 57106

Theoretical pI: Translated: 9.26; Mature: 9.26

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVRIPSVPFFSKKKLEKRLRLAPSPTGLLHIGTARTALFNWLYAKKTNGKFFIRIEDTD
CEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHEEECCCEEEEEECCCH
IVRSKSEYTTNILDGLNWLGLSWDEEPIKQSKRISIYKKNIKKLLEIGAAYRCFTSESEI
HHHCCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHH
SELREKQKSSGLPPRHDNRHRNLTSNEIKAFLSQGRSSVIRFKIDDETEIKWEDQIRGEI
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCEE
KWKGRDLGGDLVLSRRALGDEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIY
EECCCCCCCHHHHHHHHHHHHHCCCHHEEEEEEECCCEEEHEEECCCHHHHHHHHHHHHE
KALNFKLPIFSHTPLILNSEGKKLSKRDCVTSIDEFREMGYLPEALANYMAFLGWSIKDS
EECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
ESEILSLKEISRVFNLSDINKAGAKFNWEKLNWINSQYIKQMELTKLRQLIKNYWDDMGW
HHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ESPSAEWDLKLINLIRDSMLLLKDAIDQSKPFFTLSQMQKEGEEFLNNKDETRESLKYIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
CYLKEENISTIDSNKAKEIIHKISVENSIKKGILMKSLRVAFFGCLSGPDLIQSWELFSE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NKSDITLIERCLI
CCCHHHHHHHHCC
>Mature Secondary Structure
MRVRIPSVPFFSKKKLEKRLRLAPSPTGLLHIGTARTALFNWLYAKKTNGKFFIRIEDTD
CEEECCCCCCHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHEEECCCEEEEEECCCH
IVRSKSEYTTNILDGLNWLGLSWDEEPIKQSKRISIYKKNIKKLLEIGAAYRCFTSESEI
HHHCCHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCHHHH
SELREKQKSSGLPPRHDNRHRNLTSNEIKAFLSQGRSSVIRFKIDDETEIKWEDQIRGEI
HHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCEEECCCCCCEE
KWKGRDLGGDLVLSRRALGDEIGDPLYNLAVVVDDNFMNITHVVRGEDHISNTAKQILIY
EECCCCCCCHHHHHHHHHHHHHCCCHHEEEEEEECCCEEEHEEECCCHHHHHHHHHHHHE
KALNFKLPIFSHTPLILNSEGKKLSKRDCVTSIDEFREMGYLPEALANYMAFLGWSIKDS
EECCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCC
ESEILSLKEISRVFNLSDINKAGAKFNWEKLNWINSQYIKQMELTKLRQLIKNYWDDMGW
HHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
ESPSAEWDLKLINLIRDSMLLLKDAIDQSKPFFTLSQMQKEGEEFLNNKDETRESLKYIL
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHH
CYLKEENISTIDSNKAKEIIHKISVENSIKKGILMKSLRVAFFGCLSGPDLIQSWELFSE
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
NKSDITLIERCLI
CCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA