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The map label for this gene is queA

Identifier: 123965702

GI number: 123965702

Start: 415663

End: 416784

Strand: Direct

Name: queA

Synonym: P9515_04671

Alternate gene names: 123965702

Gene position: 415663-416784 (Clockwise)

Preceding gene: 123965701

Following gene: 123965707

Centisome position: 24.39

GC content: 31.46

Gene sequence:

>1122_bases
TTGAATTTTGAAATTTATAATGAAGAAATAGATCATAAGCTTGAAGCTTATGATTATTGTCTTGATGAATCATTAATAGC
AGGTAAACCCTCTAAAGTAAGGCATGAATCTAGATTAATGATAGTTAGAGATAGTTCGCTAAAAGAAGATTATTCAACAA
ATAAATATACCAAGGATCTCTTGGCTGAACTTAGAGAGGGGGATCTGGTAGTCATAAATGATACAAAGGTGATGAAAGCG
AGGTTAAAGGTTGAATTAGAAAATGGACAATTAGTGGAATTATTAGTGTTGGAGAAATCAGATCAATCTACTTGGCTATG
TTTAGCAAAGCCTGCAAAAAAGTTAAAAATAAATAAACAATTAAATTTAAAGTCACCCTTTGCGAAAGATATAAAGTTAA
AAATTTCTGGGATTGATGATGAAACTGGAGGAAGATTCATCAAGTTTCCAGAAAACATAAATGATTTAATTTCAATGAAT
AAACTCCTTGATATATTCGGAGAAATTCCAATCCCTCCATATATAAAAAGTTCTGAGGAAGAATCTTTTCATGAAAATAG
TTATCAAACTGAGTATGCATGTAATCCGGGAGCAGTTGCAGCACCCACGGCAGGATTACATTTAAGTAAAAGTCTTATCT
CAAATTTAAAAAAGAAAGGAATATTGGTTATGCCTATTACTTTGCATGTAGGTTATGGAACATTTAAGCCAATTGATCAA
GAAGATTTATCTGATTTAAAACTTCATAAAGAGTGGGTCAGTGTTAGTAAAAAAGTAGTAGAAGAAATAAAAAGAATAAA
AAAGACAGACAGACGAGTAATAGCTATTGGTACTACTAGCGTGCGAGCTCTTGAAAGTTGCTATTCATATGCAATGAAAG
ACTTTATTCCTATAGCTAAGTATGTTGATTTAGTAATTAAGCCAGGCTATAAATTTAAGGCAGTCGATGGATTATTAACC
AATTTTCATCTCCCTAAGAGTTCATTATTACTCCTAGTAAGCGCGATGATTGGTAGAGAGAGGTTATTAGATCTATATAA
AAAAGCCACAAAAGAAAAATTTAGATTTTTCTCTTATGGCGATGCTATGTATATTTCACCAGATTCATTTCTGGAGAAAT
AA

Upstream 100 bases:

>100_bases
TGATCATAGAGTGATTTATGGTGCTGATGGTGCTTCATTCTTGAAAGACTTGTCTTCTCTCATTGAAAATGAGCCAGAGA
CACTTGTTTCCTGATTTTAA

Downstream 100 bases:

>100_bases
TATTTTTAAGCGTTAATTGGTTCCTCAATTATGCCGCTTGGGACTTCAGTAAACATTATTGATGATAAATATCTTTCCGC
TAAATCAGGAAGAACAACGA

Product: queuosine biosynthesis protein

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNFEIYNEEIDHKLEAYDYCLDESLIAGKPSKVRHESRLMIVRDSSLKEDYSTNKYTKDLLAELREGDLVVINDTKVMKA
RLKVELENGQLVELLVLEKSDQSTWLCLAKPAKKLKINKQLNLKSPFAKDIKLKISGIDDETGGRFIKFPENINDLISMN
KLLDIFGEIPIPPYIKSSEEESFHENSYQTEYACNPGAVAAPTAGLHLSKSLISNLKKKGILVMPITLHVGYGTFKPIDQ
EDLSDLKLHKEWVSVSKKVVEEIKRIKKTDRRVIAIGTTSVRALESCYSYAMKDFIPIAKYVDLVIKPGYKFKAVDGLLT
NFHLPKSSLLLLVSAMIGRERLLDLYKKATKEKFRFFSYGDAMYISPDSFLEK

Sequences:

>Translated_373_residues
MNFEIYNEEIDHKLEAYDYCLDESLIAGKPSKVRHESRLMIVRDSSLKEDYSTNKYTKDLLAELREGDLVVINDTKVMKA
RLKVELENGQLVELLVLEKSDQSTWLCLAKPAKKLKINKQLNLKSPFAKDIKLKISGIDDETGGRFIKFPENINDLISMN
KLLDIFGEIPIPPYIKSSEEESFHENSYQTEYACNPGAVAAPTAGLHLSKSLISNLKKKGILVMPITLHVGYGTFKPIDQ
EDLSDLKLHKEWVSVSKKVVEEIKRIKKTDRRVIAIGTTSVRALESCYSYAMKDFIPIAKYVDLVIKPGYKFKAVDGLLT
NFHLPKSSLLLLVSAMIGRERLLDLYKKATKEKFRFFSYGDAMYISPDSFLEK
>Mature_373_residues
MNFEIYNEEIDHKLEAYDYCLDESLIAGKPSKVRHESRLMIVRDSSLKEDYSTNKYTKDLLAELREGDLVVINDTKVMKA
RLKVELENGQLVELLVLEKSDQSTWLCLAKPAKKLKINKQLNLKSPFAKDIKLKISGIDDETGGRFIKFPENINDLISMN
KLLDIFGEIPIPPYIKSSEEESFHENSYQTEYACNPGAVAAPTAGLHLSKSLISNLKKKGILVMPITLHVGYGTFKPIDQ
EDLSDLKLHKEWVSVSKKVVEEIKRIKKTDRRVIAIGTTSVRALESCYSYAMKDFIPIAKYVDLVIKPGYKFKAVDGLLT
NFHLPKSSLLLLVSAMIGRERLLDLYKKATKEKFRFFSYGDAMYISPDSFLEK

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=361, Percent_Identity=38.781163434903, Blast_Score=232, Evalue=3e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_PROM5 (A2BV65)

Other databases:

- EMBL:   CP000552
- RefSeq:   YP_001010783.1
- ProteinModelPortal:   A2BV65
- SMR:   A2BV65
- STRING:   A2BV65
- GeneID:   4719291
- GenomeReviews:   CP000552_GR
- KEGG:   pmc:P9515_04671
- eggNOG:   COG0809
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   CLSK921848
- BioCyc:   PMAR167542:P9515ORF_0501-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 42472; Mature: 42472

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFEIYNEEIDHKLEAYDYCLDESLIAGKPSKVRHESRLMIVRDSSLKEDYSTNKYTKDL
CCCEECCHHHHHHHHHHHHHHCCHHHCCCCHHCCCCCEEEEEECCCCCCCCCCCHHHHHH
LAELREGDLVVINDTKVMKARLKVELENGQLVELLVLEKSDQSTWLCLAKPAKKLKINKQ
HHHHCCCCEEEEECCCEEEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCHHEEECCC
LNLKSPFAKDIKLKISGIDDETGGRFIKFPENINDLISMNKLLDIFGEIPIPPYIKSSEE
CCCCCCCCCCEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ESFHENSYQTEYACNPGAVAAPTAGLHLSKSLISNLKKKGILVMPITLHVGYGTFKPIDQ
HHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCH
EDLSDLKLHKEWVSVSKKVVEEIKRIKKTDRRVIAIGTTSVRALESCYSYAMKDFIPIAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
YVDLVIKPGYKFKAVDGLLTNFHLPKSSLLLLVSAMIGRERLLDLYKKATKEKFRFFSYG
HHHHHCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
DAMYISPDSFLEK
CEEEECCHHHHCC
>Mature Secondary Structure
MNFEIYNEEIDHKLEAYDYCLDESLIAGKPSKVRHESRLMIVRDSSLKEDYSTNKYTKDL
CCCEECCHHHHHHHHHHHHHHCCHHHCCCCHHCCCCCEEEEEECCCCCCCCCCCHHHHHH
LAELREGDLVVINDTKVMKARLKVELENGQLVELLVLEKSDQSTWLCLAKPAKKLKINKQ
HHHHCCCCEEEEECCCEEEEEEEEEECCCCEEEEEEEECCCCCCEEEEECCCHHEEECCC
LNLKSPFAKDIKLKISGIDDETGGRFIKFPENINDLISMNKLLDIFGEIPIPPYIKSSEE
CCCCCCCCCCEEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCC
ESFHENSYQTEYACNPGAVAAPTAGLHLSKSLISNLKKKGILVMPITLHVGYGTFKPIDQ
HHHHCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEEEECCCCCCCCCH
EDLSDLKLHKEWVSVSKKVVEEIKRIKKTDRRVIAIGTTSVRALESCYSYAMKDFIPIAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHHHHH
YVDLVIKPGYKFKAVDGLLTNFHLPKSSLLLLVSAMIGRERLLDLYKKATKEKFRFFSYG
HHHHHCCCCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECC
DAMYISPDSFLEK
CEEEECCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA