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The map label for this gene is pdhC [H]

Identifier: 123965701

GI number: 123965701

Start: 414289

End: 415656

Strand: Direct

Name: pdhC [H]

Synonym: P9515_04661

Alternate gene names: 123965701

Gene position: 414289-415656 (Clockwise)

Preceding gene: 123965700

Following gene: 123965702

Centisome position: 24.31

GC content: 35.89

Gene sequence:

>1368_bases
ATGTCTCACGAAATATTTATGCCAGCTCTTAGTTCTACTATGACTGAAGGCAAAATTGTTGAATGGTTGAAAAATCCTGG
AGATAAAGTTGAAAGAGGAGAATCTGTTTTAGTCGTTGAATCAGATAAAGCTGATATGGATGTGGAATCTTTTCAAGACG
GATATCTTGCGGCAGTTCTCATGCCAGCTGGGAGCACTGCTCCAGTAGGGGAAACCATAGGACTTATTGTAGAAAATGAG
GATGAGATAGCTTCTATACAAGAACAAAATAAAGGTAAACAGATTGAAGTATCCAGTGATGCCCAACTCAAATTGCCAAA
TAAAAAATCTGAAATAATAGAAGAGAAGCAAAAAGAGCTGCCTCAAATTAATGAGCAGCAAGTCGAGATTAAAAGGGAAA
AAGTGATAAATACTTCTAATGAAATTCAATTTAATGCTTCTACTAGTAATAATTCCTCGAGAGTAATAGCATCTCCAAGG
GCAAAAAAACTTGCTTCAACAATGGGAGTTGAATTGACAAAAGTGCATGGATCAGGACCTCATGGAAGGATTCAGGCTGA
GGATGTATTGAAGGCAAATGGGCAACCTGTTTCTATCCCATGGATTGGAGAGGGGAGTTCTCCAGCTAGTATTGGCAGTT
CCCATGTTCAAGCTGAAAGTAAGTCAGAGACATTAGGGAATAGTTTTGGTAAACCTGGAGAAACTGTTAAATTTAATACT
CTTCAAAAAGCTGTTAATAACAATATGGAATCTAGTTTAAATGTTCCATGTTTTAGAGTTGGTTATTCAATTAATACTGA
TAAATTGGATAATTTTTATAAGAAAGTAAAGCAAAATGGTGTTACTATGACTGCATTATTAGTCAAGGCCGTTGCAAAGA
CACTTAAAAAACATCCCCAAGTTAATTCAAGCTTTTCAGAAAACGGAATTTCTTATCCAGAAAATATAAATATTGCTGTA
GCTGTTGCTATGGAAGATGGCGGATTGATAACTCCAGTATTAAAAGAACCTTGTAACACTGATTTATTTGAATTATCTAG
AGAGTGGAAAGACCTTGTGAAAAGATCTAGAGCAAAACAATTAGAACCTGATGAATACTCAACAGGAACATTTACGTTGT
CTAATTTAGGAATGTTTGGTGTTGATAGATTTGATGCAATACTTCCACCAGGTACGGGTGCTATTTTAGCCATAGCATCA
TCTAAGCCAACAGTCGTTGCTAACAATGATGGTTCAATATCTGTTAAAAAAATAATGCAAGTAAATTTAACCGCTGATCA
TAGAGTGATTTATGGTGCTGATGGTGCTTCATTCTTGAAAGACTTGTCTTCTCTCATTGAAAATGAGCCAGAGACACTTG
TTTCCTGA

Upstream 100 bases:

>100_bases
TCATAGTTTCCTTAGCGCTATTTTTTTGTAAAATATAAACATAACTATCTTTATTAATTTTGTAAGTTCTTAAAGTTAGT
AAATTTTTAATATCTATCTG

Downstream 100 bases:

>100_bases
TTTTAATTGAATTTTGAAATTTATAATGAAGAAATAGATCATAAGCTTGAAGCTTATGATTATTGTCTTGATGAATCATT
AATAGCAGGTAAACCCTCTA

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENE
DEIASIQEQNKGKQIEVSSDAQLKLPNKKSEIIEEKQKELPQINEQQVEIKREKVINTSNEIQFNASTSNNSSRVIASPR
AKKLASTMGVELTKVHGSGPHGRIQAEDVLKANGQPVSIPWIGEGSSPASIGSSHVQAESKSETLGNSFGKPGETVKFNT
LQKAVNNNMESSLNVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQVNSSFSENGISYPENINIAV
AVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRAKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIAS
SKPTVVANNDGSISVKKIMQVNLTADHRVIYGADGASFLKDLSSLIENEPETLVS

Sequences:

>Translated_455_residues
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENE
DEIASIQEQNKGKQIEVSSDAQLKLPNKKSEIIEEKQKELPQINEQQVEIKREKVINTSNEIQFNASTSNNSSRVIASPR
AKKLASTMGVELTKVHGSGPHGRIQAEDVLKANGQPVSIPWIGEGSSPASIGSSHVQAESKSETLGNSFGKPGETVKFNT
LQKAVNNNMESSLNVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQVNSSFSENGISYPENINIAV
AVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRAKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIAS
SKPTVVANNDGSISVKKIMQVNLTADHRVIYGADGASFLKDLSSLIENEPETLVS
>Mature_454_residues
SHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVLMPAGSTAPVGETIGLIVENED
EIASIQEQNKGKQIEVSSDAQLKLPNKKSEIIEEKQKELPQINEQQVEIKREKVINTSNEIQFNASTSNNSSRVIASPRA
KKLASTMGVELTKVHGSGPHGRIQAEDVLKANGQPVSIPWIGEGSSPASIGSSHVQAESKSETLGNSFGKPGETVKFNTL
QKAVNNNMESSLNVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQVNSSFSENGISYPENINIAVA
VAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRAKQLEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASS
KPTVVANNDGSISVKKIMQVNLTADHRVIYGADGASFLKDLSSLIENEPETLVS

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=466, Percent_Identity=27.6824034334764, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI203098753, Length=479, Percent_Identity=28.6012526096033, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI203098816, Length=474, Percent_Identity=28.9029535864979, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI110671329, Length=451, Percent_Identity=26.6075388026608, Blast_Score=108, Evalue=7e-24,
Organism=Homo sapiens, GI260898739, Length=156, Percent_Identity=33.974358974359, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI19923748, Length=172, Percent_Identity=31.3953488372093, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1786946, Length=461, Percent_Identity=23.4273318872017, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1786305, Length=302, Percent_Identity=30.4635761589404, Blast_Score=115, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI17560088, Length=466, Percent_Identity=29.8283261802575, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=451, Percent_Identity=25.4988913525499, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=29.4117647058824, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI25146366, Length=180, Percent_Identity=35, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324258, Length=465, Percent_Identity=31.1827956989247, Blast_Score=187, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6320352, Length=191, Percent_Identity=30.3664921465969, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6321632, Length=97, Percent_Identity=42.2680412371134, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24582497, Length=312, Percent_Identity=30.1282051282051, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI20129315, Length=312, Percent_Identity=30.1282051282051, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859875, Length=316, Percent_Identity=29.746835443038, Blast_Score=108, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24645909, Length=198, Percent_Identity=29.7979797979798, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 49234; Mature: 49103

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVL
CCCCEECHHHHHHCCCCHHHHHHCCCCCHHHCCCEEEEEECCCCCCCHHHHCCCEEEEEE
MPAGSTAPVGETIGLIVENEDEIASIQEQNKGKQIEVSSDAQLKLPNKKSEIIEEKQKEL
ECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCEEEECCCCEEECCCCHHHHHHHHHHHC
PQINEQQVEIKREKVINTSNEIQFNASTSNNSSRVIASPRAKKLASTMGVELTKVHGSGP
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHCCEEEEEECCCC
HGRIQAEDVLKANGQPVSIPWIGEGSSPASIGSSHVQAESKSETLGNSFGKPGETVKFNT
CCCEEHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHCCCCHHHHHHHCCCCCCCEEEHHH
LQKAVNNNMESSLNVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQ
HHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
VNSSFSENGISYPENINIAVAVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRAKQ
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
LEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASSKPTVVANNDGSISVKKIMQ
CCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEEEEE
VNLTADHRVIYGADGASFLKDLSSLIENEPETLVS
EECCCCCEEEEECCHHHHHHHHHHHHCCCCHHHCC
>Mature Secondary Structure 
SHEIFMPALSSTMTEGKIVEWLKNPGDKVERGESVLVVESDKADMDVESFQDGYLAAVL
CCCEECHHHHHHCCCCHHHHHHCCCCCHHHCCCEEEEEECCCCCCCHHHHCCCEEEEEE
MPAGSTAPVGETIGLIVENEDEIASIQEQNKGKQIEVSSDAQLKLPNKKSEIIEEKQKEL
ECCCCCCCCCCEEEEEEECCHHHHHHHHHCCCCEEEECCCCEEECCCCHHHHHHHHHHHC
PQINEQQVEIKREKVINTSNEIQFNASTSNNSSRVIASPRAKKLASTMGVELTKVHGSGP
CCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCEEEECCHHHHHHHHHCCEEEEEECCCC
HGRIQAEDVLKANGQPVSIPWIGEGSSPASIGSSHVQAESKSETLGNSFGKPGETVKFNT
CCCEEHHHHHHCCCCEEEEEEECCCCCCCCCCCHHHCCCCHHHHHHHCCCCCCCEEEHHH
LQKAVNNNMESSLNVPCFRVGYSINTDKLDNFYKKVKQNGVTMTALLVKAVAKTLKKHPQ
HHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
VNSSFSENGISYPENINIAVAVAMEDGGLITPVLKEPCNTDLFELSREWKDLVKRSRAKQ
CCCCCCCCCCCCCCCCCEEEEEEECCCCEECHHHHCCCCCHHHHHHHHHHHHHHHHHHCC
LEPDEYSTGTFTLSNLGMFGVDRFDAILPPGTGAILAIASSKPTVVANNDGSISVKKIMQ
CCCCCCCCCEEECCCCCCCCCCHHHHCCCCCCCEEEEEECCCCEEEECCCCCEEEEEEEE
VNLTADHRVIYGADGASFLKDLSSLIENEPETLVS
EECCCCCEEEEECCHHHHHHHHHHHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA