Click here to switch to the map view.

The map label for this gene is gshB [H]

Identifier: 123965442

GI number: 123965442

Start: 189558

End: 190481

Strand: Reverse

Name: gshB [H]

Synonym: P9515_02071

Alternate gene names: 123965442

Gene position: 190481-189558 (Counterclockwise)

Preceding gene: 123965443

Following gene: 123965440

Centisome position: 11.18

GC content: 33.23

Gene sequence:

>924_bases
ATGAAATTTCTATTTGTAGTCGACCCAATAAAAAATATAAATCCTTTAAAAGATTCATCTGCCGCATTAATGCAGGCTTC
TTCTAAGAAAAAGATAAAAGTTTGGACTTGCACTCCTCAAGATTTAGAGGCAAGAGGGGATGAAGTATGGGCATCTTCTT
TTAGAGCTGAAGTGATTCCGTGGGTTTCGTTTAAGGAAAATAAATGTATCCCCCTTGCTGAATTTAATTGTATTTGGATG
AGAAAAGATCCCCCTGTAAATGAGGGATATTTATATGCAACACATCTTTTAGAAGTTGCCGAAAGAAAAGGAGTAAAAGT
TATAAATAAACCTTCCTCATTACGTGCTTGGAACGAAAAACTAGGAGCATTGAGATACAGCCATTTGATGGCCCCAACAA
TAGTTGCAAGTAAAGTAAAAGATCTTATTAATTTTGCACAAATTAATGATGATGTTGTTGTGAAACCACTTGGGGGGAAA
GGAGGTCAAGGCGTAATTCGTCTAAATAAGGATTCTCCAGGTATTAAAGCAATGATTGAATTAATAACATCACAAGAAGA
ATTACCAGTAATGATGCAAAAATTTATTCCCGAAGTAAACAAAGGGGATAAAAGAATAATTATTGTTAACGGCGAGCCAA
TTGGATCCATTAATAGAATTCCTCAAGGGGGTGATTTTAGGAGTAATTTAGCTATGGGAGGTAAAGCAGAAAAAACAAAT
CTAACAGCTAAAGAAAAAAAAATTTGTACTGAGTTATCTCAACACTTCAAAGATGAAGGGTTATTCTTTGTTGGTATAGA
TGTAATCAATGAGATGCTTAGTGAAATTAATGTAACAAGTCCAACAGGATTAAGAGAGATAGAAAATTTATCTAATAAAA
AAGTTTCAGATCAAGTTATTGAAAAACTATTAGAGATTATTTAG

Upstream 100 bases:

>100_bases
TTCCTCAAATCTTTATTAATAATAAAAGTATTGGTGGTTGTGACGAACTTTATGAATTAGAAAAAGAAGAGAAACTAGAC
TTATTACTGAATTAGATAAT

Downstream 100 bases:

>100_bases
TGGTTTTGGCAATAAATATTTGCTTTACTAAAGATGTAAATTTAAGTTTAATCTCTTCAATTTCTTTGCTCGCGATAGAA
CCTTTTACTATACCAGAACC

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MKFLFVVDPIKNINPLKDSSAALMQASSKKKIKVWTCTPQDLEARGDEVWASSFRAEVIPWVSFKENKCIPLAEFNCIWM
RKDPPVNEGYLYATHLLEVAERKGVKVINKPSSLRAWNEKLGALRYSHLMAPTIVASKVKDLINFAQINDDVVVKPLGGK
GGQGVIRLNKDSPGIKAMIELITSQEELPVMMQKFIPEVNKGDKRIIIVNGEPIGSINRIPQGGDFRSNLAMGGKAEKTN
LTAKEKKICTELSQHFKDEGLFFVGIDVINEMLSEINVTSPTGLREIENLSNKKVSDQVIEKLLEII

Sequences:

>Translated_307_residues
MKFLFVVDPIKNINPLKDSSAALMQASSKKKIKVWTCTPQDLEARGDEVWASSFRAEVIPWVSFKENKCIPLAEFNCIWM
RKDPPVNEGYLYATHLLEVAERKGVKVINKPSSLRAWNEKLGALRYSHLMAPTIVASKVKDLINFAQINDDVVVKPLGGK
GGQGVIRLNKDSPGIKAMIELITSQEELPVMMQKFIPEVNKGDKRIIIVNGEPIGSINRIPQGGDFRSNLAMGGKAEKTN
LTAKEKKICTELSQHFKDEGLFFVGIDVINEMLSEINVTSPTGLREIENLSNKKVSDQVIEKLLEII
>Mature_307_residues
MKFLFVVDPIKNINPLKDSSAALMQASSKKKIKVWTCTPQDLEARGDEVWASSFRAEVIPWVSFKENKCIPLAEFNCIWM
RKDPPVNEGYLYATHLLEVAERKGVKVINKPSSLRAWNEKLGALRYSHLMAPTIVASKVKDLINFAQINDDVVVKPLGGK
GGQGVIRLNKDSPGIKAMIELITSQEELPVMMQKFIPEVNKGDKRIIIVNGEPIGSINRIPQGGDFRSNLAMGGKAEKTN
LTAKEKKICTELSQHFKDEGLFFVGIDVINEMLSEINVTSPTGLREIENLSNKKVSDQVIEKLLEII

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=293, Percent_Identity=39.2491467576792, Blast_Score=214, Evalue=5e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34243; Mature: 34243

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLFVVDPIKNINPLKDSSAALMQASSKKKIKVWTCTPQDLEARGDEVWASSFRAEVIP
CEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCC
WVSFKENKCIPLAEFNCIWMRKDPPVNEGYLYATHLLEVAERKGVKVINKPSSLRAWNEK
CEECCCCCCCEEECCCEEEEECCCCCCCCEEHHHHHHHHHHHCCCEEECCCHHHHHHHHH
LGALRYSHLMAPTIVASKVKDLINFAQINDDVVVKPLGGKGGQGVIRLNKDSPGIKAMIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHH
LITSQEELPVMMQKFIPEVNKGDKRIIIVNGEPIGSINRIPQGGDFRSNLAMGGKAEKTN
HHHCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
LTAKEKKICTELSQHFKDEGLFFVGIDVINEMLSEINVTSPTGLREIENLSNKKVSDQVI
CCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHH
EKLLEII
HHHHHHC
>Mature Secondary Structure
MKFLFVVDPIKNINPLKDSSAALMQASSKKKIKVWTCTPQDLEARGDEVWASSFRAEVIP
CEEEEEECCCCCCCCCCCCHHHHHHCCCCCEEEEEECCCHHHHCCCCHHHHHHHHHHHCC
WVSFKENKCIPLAEFNCIWMRKDPPVNEGYLYATHLLEVAERKGVKVINKPSSLRAWNEK
CEECCCCCCCEEECCCEEEEECCCCCCCCEEHHHHHHHHHHHCCCEEECCCHHHHHHHHH
LGALRYSHLMAPTIVASKVKDLINFAQINDDVVVKPLGGKGGQGVIRLNKDSPGIKAMIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCCHHHHHHH
LITSQEELPVMMQKFIPEVNKGDKRIIIVNGEPIGSINRIPQGGDFRSNLAMGGKAEKTN
HHHCCCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHCCCCCCCCCCCC
LTAKEKKICTELSQHFKDEGLFFVGIDVINEMLSEINVTSPTGLREIENLSNKKVSDQVI
CCHHHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHCCCCHHHHHH
EKLLEII
HHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]