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The map label for this gene is rfbB [H]

Identifier: 123965387

GI number: 123965387

Start: 137254

End: 138327

Strand: Reverse

Name: rfbB [H]

Synonym: P9515_01521

Alternate gene names: 123965387

Gene position: 138327-137254 (Counterclockwise)

Preceding gene: 123965389

Following gene: 123965385

Centisome position: 8.12

GC content: 31.19

Gene sequence:

>1074_bases
ATGATAAAAAACAAATATAAGATAATTGTTACGGGAGGAGCAGGCTTTATTGGAGGGACTTTAATAAGAAAGATATTGCA
AGAAAAAAAAGACTGGGTTGTTTATAACTTGGATCAAATGGGCTATGCAAGTGACTTATCGTGGATAAAAAATTCAGAGG
ACTATAAATTTAGACATTTCTTCTTGAAAATTGATTTAAGGAACAAAGAAATATTAGAAAATTTAGTCAAAGAGATAAAT
CCTAATTTGATAATACATCTTGCAGCTGAAAGTCATGTTGATCGATCAATTGATAACCCATTAAAATTTATAGAGAGCAA
CATCATAGGAACTTTTAATTTACTTGAGGCCTCAAGAGCTTATTGGAAAAAATTAAGTGATAATAAAAAACAATTATTTA
GATTTATTCACATAAGCACAGATGAAGTCTTCGGATCATTGGGATTAGATGGAAAATTTGATGAGAATACTAAATATTCT
CCTCGAAGTCCATATTCTGCTAGTAAAGCCTCTAGTGATCATTTGGCACAAAGTTGGTACCATACATATGGTTTACCTAC
TATAATTTCGAATTGTAGTAACAACTTTGGGCCATATCAATTCCCAGAAAAACTTATCCCTTTAAGTATTTTAAAAGGAA
TTAAAGGGGAAAAAATACCTCTTTATGGAAATGGTTTAAATGTAAGAGATTGGTTATATGTAGAAGATCATGTAGAAGCT
TTATTACTTATCGCTGAAAAGGGAGAAGTTGGAAAGAGTTACTGTATAGGGGGATTTGGTGAGAGAACTAATAAAGAAGT
TCAACTTCAAATATGTAAAATTCTTGATGCGGTTGCTCCCAAAGAATTTCCTCATGAATCTCTTATTAAAAATGTTACAG
ACAGACCGGGGCATGATATTAGATACGCCATAAATTCAAATTTAATCAAAGAAAAGTTAGGTTGGACTCCTAAAGTAACT
TTTGAAGATGGGTTAAAAAAAACTATTTATTGGTATCTGAATCACTCTAAGTGGATTCATTCAATGATGCAAAAATCAGG
TTACGCAGGCAATAGAATTGGAAATAAGAAATAA

Upstream 100 bases:

>100_bases
TATAATTATTGCTATTAATAAATAAGAGCTAATTTAGGAATATCCTATCATTATTTAAAGTTTCTTTTATAAAGAAAAAT
TTATTTATTTTACATATTTC

Downstream 100 bases:

>100_bases
ATATTAAAGTTGATTCATACCTAAAGATAATTCCTTTTGAGATGCTTTAATGACTTTCAAAGAAGATTTGATAAAAACGA
ATGTCAGTAGAAAACCAACC

Product: dTDP-D-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MIKNKYKIIVTGGAGFIGGTLIRKILQEKKDWVVYNLDQMGYASDLSWIKNSEDYKFRHFFLKIDLRNKEILENLVKEIN
PNLIIHLAAESHVDRSIDNPLKFIESNIIGTFNLLEASRAYWKKLSDNKKQLFRFIHISTDEVFGSLGLDGKFDENTKYS
PRSPYSASKASSDHLAQSWYHTYGLPTIISNCSNNFGPYQFPEKLIPLSILKGIKGEKIPLYGNGLNVRDWLYVEDHVEA
LLLIAEKGEVGKSYCIGGFGERTNKEVQLQICKILDAVAPKEFPHESLIKNVTDRPGHDIRYAINSNLIKEKLGWTPKVT
FEDGLKKTIYWYLNHSKWIHSMMQKSGYAGNRIGNKK

Sequences:

>Translated_357_residues
MIKNKYKIIVTGGAGFIGGTLIRKILQEKKDWVVYNLDQMGYASDLSWIKNSEDYKFRHFFLKIDLRNKEILENLVKEIN
PNLIIHLAAESHVDRSIDNPLKFIESNIIGTFNLLEASRAYWKKLSDNKKQLFRFIHISTDEVFGSLGLDGKFDENTKYS
PRSPYSASKASSDHLAQSWYHTYGLPTIISNCSNNFGPYQFPEKLIPLSILKGIKGEKIPLYGNGLNVRDWLYVEDHVEA
LLLIAEKGEVGKSYCIGGFGERTNKEVQLQICKILDAVAPKEFPHESLIKNVTDRPGHDIRYAINSNLIKEKLGWTPKVT
FEDGLKKTIYWYLNHSKWIHSMMQKSGYAGNRIGNKK
>Mature_357_residues
MIKNKYKIIVTGGAGFIGGTLIRKILQEKKDWVVYNLDQMGYASDLSWIKNSEDYKFRHFFLKIDLRNKEILENLVKEIN
PNLIIHLAAESHVDRSIDNPLKFIESNIIGTFNLLEASRAYWKKLSDNKKQLFRFIHISTDEVFGSLGLDGKFDENTKYS
PRSPYSASKASSDHLAQSWYHTYGLPTIISNCSNNFGPYQFPEKLIPLSILKGIKGEKIPLYGNGLNVRDWLYVEDHVEA
LLLIAEKGEVGKSYCIGGFGERTNKEVQLQICKILDAVAPKEFPHESLIKNVTDRPGHDIRYAINSNLIKEKLGWTPKVT
FEDGLKKTIYWYLNHSKWIHSMMQKSGYAGNRIGNKK

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=34.0425531914894, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI42516563, Length=337, Percent_Identity=25.2225519287834, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI48994969, Length=359, Percent_Identity=52.3676880222841, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1788353, Length=353, Percent_Identity=50.4249291784703, Blast_Score=363, Evalue=1e-102,
Organism=Escherichia coli, GI1788366, Length=315, Percent_Identity=26.984126984127, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786974, Length=350, Percent_Identity=22.2857142857143, Blast_Score=71, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17568069, Length=328, Percent_Identity=32.9268292682927, Blast_Score=181, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI115532424, Length=363, Percent_Identity=28.6501377410468, Blast_Score=159, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17539532, Length=350, Percent_Identity=23.1428571428571, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17507723, Length=280, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=291, Percent_Identity=25.4295532646048, Blast_Score=69, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=291, Percent_Identity=25.4295532646048, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=291, Percent_Identity=25.4295532646048, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=291, Percent_Identity=25.4295532646048, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=291, Percent_Identity=25.4295532646048, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=356, Percent_Identity=24.438202247191, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356223, Length=338, Percent_Identity=24.2603550295858, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24158427, Length=346, Percent_Identity=23.9884393063584, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI19923002, Length=355, Percent_Identity=22.8169014084507, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 40908; Mature: 40908

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNKYKIIVTGGAGFIGGTLIRKILQEKKDWVVYNLDQMGYASDLSWIKNSEDYKFRHF
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHCCCHHHHHHHCCCCCCEEEEE
FLKIDLRNKEILENLVKEINPNLIIHLAAESHVDRSIDNPLKFIESNIIGTFNLLEASRA
EEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YWKKLSDNKKQLFRFIHISTDEVFGSLGLDGKFDENTKYSPRSPYSASKASSDHLAQSWY
HHHHHCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HTYGLPTIISNCSNNFGPYQFPEKLIPLSILKGIKGEKIPLYGNGLNVRDWLYVEDHVEA
HHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEHHHHEE
LLLIAEKGEVGKSYCIGGFGERTNKEVQLQICKILDAVAPKEFPHESLIKNVTDRPGHDI
EEEEECCCCCCCHHEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCE
RYAINSNLIKEKLGWTPKVTFEDGLKKTIYWYLNHSKWIHSMMQKSGYAGNRIGNKK
EEECCCHHHHHHHCCCCCEEHHHCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MIKNKYKIIVTGGAGFIGGTLIRKILQEKKDWVVYNLDQMGYASDLSWIKNSEDYKFRHF
CCCCCEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHCCCHHHHHHHCCCCCCEEEEE
FLKIDLRNKEILENLVKEINPNLIIHLAAESHVDRSIDNPLKFIESNIIGTFNLLEASRA
EEEEECCCHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
YWKKLSDNKKQLFRFIHISTDEVFGSLGLDGKFDENTKYSPRSPYSASKASSDHLAQSWY
HHHHHCCCHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
HTYGLPTIISNCSNNFGPYQFPEKLIPLSILKGIKGEKIPLYGNGLNVRDWLYVEDHVEA
HHCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEHHHHEE
LLLIAEKGEVGKSYCIGGFGERTNKEVQLQICKILDAVAPKEFPHESLIKNVTDRPGHDI
EEEEECCCCCCCHHEECCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCE
RYAINSNLIKEKLGWTPKVTFEDGLKKTIYWYLNHSKWIHSMMQKSGYAGNRIGNKK
EEECCCHHHHHHHCCCCCEEHHHCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 7559340 [H]