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The map label for this gene is speB [C]

Identifier: 123969428

GI number: 123969428

Start: 1631137

End: 1632018

Strand: Direct

Name: speB [C]

Synonym: A9601_18961

Alternate gene names: 123969428

Gene position: 1631137-1632018 (Clockwise)

Preceding gene: 123969427

Following gene: 123969429

Centisome position: 97.68

GC content: 33.33

Gene sequence:

>882_bases
ATGACAAAAAATTTATTTGATAACGAAAATGCAATTTATATGGGAGCAAAAAGAAGTCCCGAAAATTGCTCAATTGGAAT
ATTTGGAGTTAATTATGACGGGACATGTTCGTTTAAACCAGGAGCAAGATTTGGTCCAGAAGCAATAAGACAAGTCAGTT
CTTGTTTAGAAACATATTGTCCAAAAATTAAAAAAGACTTAGAGGATATTATGTATGTTGATTTTGGATCAATACTAATT
GATAAAAATGACTCAAAGTCCGTTATTGAATCGGTTAAATCAGCAACAAATTATTTAATCAGTAAACGCCTTAGTCCTAT
TATGCTCGGCGGCGAACACTCTATTACAAGAGGTGCAATTGAAGCATTAGTAAAAAAATATCCAGATTTGATATTGGTTC
AACTTGATGCTCATGCAGATTTAAGAGAATCATATATAGGAAATGAACATAGTCATGCTTGTACTATGAAAAGGTGCTTA
GAAGTGCTACCTGAAAAGAAAATTTTGCAAGTAGGAATTAGAAGTGGGACTAAGGAAGAATTTGAAATTATGCATAACAA
CAACCAATTAGTTAACTTTTGTCCAGGCGGAAATGCATATGAGTTAAAACAAGCTCTTCTTCCATACGCTAAGTGTCCAA
TCTATTTAACAATAGATTTAGATTGGTTTGATCCCAGTTTATTAGCAGGGACGGGCACTCCAGAACCGGGAGGATTTTTT
TGGAATGATTTTGAAGAGATACTTAAAACTTTAAAAAACCTTAGAATCGTGGGATCAGATATTGTGGAATTATCTCCAGA
AATTGATAGAAGCGGAGTAAGTAGCATAGTTGCAGCCAAAGTACTTAGAAGCTTAATTTTGTCATTAGAAAATATGCAAT
AA

Upstream 100 bases:

>100_bases
GTAAAAATAGAAAAAGGATGTGAAATTTGGAATTTAAATTTTCAAAATGCAGCATTCAAAATGATGCCAAATAAAATTGT
AAAAGAACTAGATTCATAAG

Downstream 100 bases:

>100_bases
AAAATTTCTAAACTAAAGTGTATAAATACTAAATAGAAACTAAATATTTCATGGATTTGCTAAAAAGTCCTCTTTATTCA
AAATATGTTGAATCCAATGC

Product: arginase

Products: putrescine; urea

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTKNLFDNENAIYMGAKRSPENCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKIKKDLEDIMYVDFGSILI
DKNDSKSVIESVKSATNYLISKRLSPIMLGGEHSITRGAIEALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCL
EVLPEKKILQVGIRSGTKEEFEIMHNNNQLVNFCPGGNAYELKQALLPYAKCPIYLTIDLDWFDPSLLAGTGTPEPGGFF
WNDFEEILKTLKNLRIVGSDIVELSPEIDRSGVSSIVAAKVLRSLILSLENMQ

Sequences:

>Translated_293_residues
MTKNLFDNENAIYMGAKRSPENCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKIKKDLEDIMYVDFGSILI
DKNDSKSVIESVKSATNYLISKRLSPIMLGGEHSITRGAIEALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCL
EVLPEKKILQVGIRSGTKEEFEIMHNNNQLVNFCPGGNAYELKQALLPYAKCPIYLTIDLDWFDPSLLAGTGTPEPGGFF
WNDFEEILKTLKNLRIVGSDIVELSPEIDRSGVSSIVAAKVLRSLILSLENMQ
>Mature_292_residues
TKNLFDNENAIYMGAKRSPENCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYCPKIKKDLEDIMYVDFGSILID
KNDSKSVIESVKSATNYLISKRLSPIMLGGEHSITRGAIEALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCLE
VLPEKKILQVGIRSGTKEEFEIMHNNNQLVNFCPGGNAYELKQALLPYAKCPIYLTIDLDWFDPSLLAGTGTPEPGGFFW
NDFEEILKTLKNLRIVGSDIVELSPEIDRSGVSSIVAAKVLRSLILSLENMQ

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI37537722, Length=288, Percent_Identity=28.8194444444444, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1789306, Length=256, Percent_Identity=29.296875, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005925
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: 3.5.3.11

Molecular weight: Translated: 32591; Mature: 32460

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS00012 PHOSPHOPANTETHEINE ; PS00147 ARGINASE_1 ; PS00148 ARGINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNLFDNENAIYMGAKRSPENCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYC
CCCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHC
PKIKKDLEDIMYVDFGSILIDKNDSKSVIESVKSATNYLISKRLSPIMLGGEHSITRGAI
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHH
EALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCLEVLPEKKILQVGIRSGTKEE
HHHHHHCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHH
FEIMHNNNQLVNFCPGGNAYELKQALLPYAKCPIYLTIDLDWFDPSLLAGTGTPEPGGFF
HHEEECCCCEEEECCCCCHHHHHHHHCCHHCCCEEEEEEECCCCCCCEECCCCCCCCCCC
WNDFEEILKTLKNLRIVGSDIVELSPEIDRSGVSSIVAAKVLRSLILSLENMQ
HHHHHHHHHHHHHCEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKNLFDNENAIYMGAKRSPENCSIGIFGVNYDGTCSFKPGARFGPEAIRQVSSCLETYC
CCCCCCCCCEEEEECCCCCCCCEEEEEEECCCCEECCCCCCCCCHHHHHHHHHHHHHHC
PKIKKDLEDIMYVDFGSILIDKNDSKSVIESVKSATNYLISKRLSPIMLGGEHSITRGAI
HHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHH
EALVKKYPDLILVQLDAHADLRESYIGNEHSHACTMKRCLEVLPEKKILQVGIRSGTKEE
HHHHHHCCCEEEEEECCCCHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCHHH
FEIMHNNNQLVNFCPGGNAYELKQALLPYAKCPIYLTIDLDWFDPSLLAGTGTPEPGGFF
HHEEECCCCEEEECCCCCHHHHHHHHCCHHCCCEEEEEEECCCCCCCEECCCCCCCCCCC
WNDFEEILKTLKNLRIVGSDIVELSPEIDRSGVSSIVAAKVLRSLILSLENMQ
HHHHHHHHHHHHHCEECCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: agmatine; H2O

Specific reaction: agmatine + H2O = putrescine + urea

General reaction: Amidine hydrolysis [C]

Inhibitor: EDTA; EGTA [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]