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The map label for this gene is speE [H]

Identifier: 123969427

GI number: 123969427

Start: 1630284

End: 1631135

Strand: Direct

Name: speE [H]

Synonym: A9601_18951

Alternate gene names: 123969427

Gene position: 1630284-1631135 (Clockwise)

Preceding gene: 123969426

Following gene: 123969428

Centisome position: 97.63

GC content: 29.11

Gene sequence:

>852_bases
ATGACAGATATTACAACATGGATAGATGAATATCATAAAGGCTCAAGATTCGGCCTAAATGGGAAAATTCTATTTAAAAA
AACCTCAAAATATCAAGAAATTATTGTTATTGAAAATGAATATTATGGTAAAGCTTTAATGTTAGATGGTTGCTGGATGA
CATCATTAAAAGACGAGAAATATTATCATGAGTGTCTTGTGCATCCTGCATTAAGTAGCATTGACGAAAAATCTAATGTA
CTAATTATTGGCGGTGGTGACGGTGGTACTGTAAGAGAATGCGTTAAATATTCTAAAATATCAAAAATTGATCTAGTAGA
AATTGATGAGGAGGTAATCAAAATATCTAAAAAATTTCTAAAAGAAATTGCAGGCGAAGCATGGAATGACAAAAGATTAG
AAATACATATTGATGATGGCGTTAAATGGGTAGAAAAAACAAAAGATAATTTTTACGACGTTATATTTATAGATTGTTCA
GATCCCTCAGAATTTTCAAATTTATTATTTTCACATTCTTTTTATAAAGAATGTGAAAGAATACTTGCACCATCGGGGAT
ATTAGCAACTCAAAGCGAATCTCCTGAATCCTTCAAAAATATTCACATAAATATTTTGAAAAGCCTAAAAAATATATTTA
AAGTTTCTGAAACTATGTATTCCTTTGTGCCTATATATCCAAGCGGGATTTGGAGTTGGACATTTGCTTCTTCAGAAGAT
CTAAGTTTATCAAAGCAAAATTATGATGAAGTCGTAAAAATAGAAAAAGGATGTGAAATTTGGAATTTAAATTTTCAAAA
TGCAGCATTCAAAATGATGCCAAATAAAATTGTAAAAGAACTAGATTCATAA

Upstream 100 bases:

>100_bases
AGCATGCAGGAGATAATATTAAAAAACAAACTCCTAAAGACTTTCAACGGCTTTGGCAAATAGCCAAACAAAAACTGGCG
GGAAAAATTCCTAAAAGCAA

Downstream 100 bases:

>100_bases
GATGACAAAAAATTTATTTGATAACGAAAATGCAATTTATATGGGAGCAAAAAGAAGTCCCGAAAATTGCTCAATTGGAA
TATTTGGAGTTAATTATGAC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MTDITTWIDEYHKGSRFGLNGKILFKKTSKYQEIIVIENEYYGKALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNV
LIIGGGDGGTVRECVKYSKISKIDLVEIDEEVIKISKKFLKEIAGEAWNDKRLEIHIDDGVKWVEKTKDNFYDVIFIDCS
DPSEFSNLLFSHSFYKECERILAPSGILATQSESPESFKNIHINILKSLKNIFKVSETMYSFVPIYPSGIWSWTFASSED
LSLSKQNYDEVVKIEKGCEIWNLNFQNAAFKMMPNKIVKELDS

Sequences:

>Translated_283_residues
MTDITTWIDEYHKGSRFGLNGKILFKKTSKYQEIIVIENEYYGKALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNV
LIIGGGDGGTVRECVKYSKISKIDLVEIDEEVIKISKKFLKEIAGEAWNDKRLEIHIDDGVKWVEKTKDNFYDVIFIDCS
DPSEFSNLLFSHSFYKECERILAPSGILATQSESPESFKNIHINILKSLKNIFKVSETMYSFVPIYPSGIWSWTFASSED
LSLSKQNYDEVVKIEKGCEIWNLNFQNAAFKMMPNKIVKELDS
>Mature_282_residues
TDITTWIDEYHKGSRFGLNGKILFKKTSKYQEIIVIENEYYGKALMLDGCWMTSLKDEKYYHECLVHPALSSIDEKSNVL
IIGGGDGGTVRECVKYSKISKIDLVEIDEEVIKISKKFLKEIAGEAWNDKRLEIHIDDGVKWVEKTKDNFYDVIFIDCSD
PSEFSNLLFSHSFYKECERILAPSGILATQSESPESFKNIHINILKSLKNIFKVSETMYSFVPIYPSGIWSWTFASSEDL
SLSKQNYDEVVKIEKGCEIWNLNFQNAAFKMMPNKIVKELDS

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=268, Percent_Identity=32.089552238806, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1786312, Length=290, Percent_Identity=30, Blast_Score=146, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI212645616, Length=230, Percent_Identity=34.3478260869565, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25154106, Length=225, Percent_Identity=36, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325326, Length=268, Percent_Identity=33.2089552238806, Blast_Score=144, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323175, Length=230, Percent_Identity=34.3478260869565, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645443, Length=263, Percent_Identity=31.5589353612167, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355941, Length=196, Percent_Identity=31.6326530612245, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 32589; Mature: 32457

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1 ; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDITTWIDEYHKGSRFGLNGKILFKKTSKYQEIIVIENEYYGKALMLDGCWMTSLKDEK
CCCHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCEECCCCCHH
YYHECLVHPALSSIDEKSNVLIIGGGDGGTVRECVKYSKISKIDLVEIDEEVIKISKKFL
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHH
KEIAGEAWNDKRLEIHIDDGVKWVEKTKDNFYDVIFIDCSDPSEFSNLLFSHSFYKECER
HHHCCCCCCCCEEEEEECCCCHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
ILAPSGILATQSESPESFKNIHINILKSLKNIFKVSETMYSFVPIYPSGIWSWTFASSED
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCC
LSLSKQNYDEVVKIEKGCEIWNLNFQNAAFKMMPNKIVKELDS
CCCCCCCHHHHHHHHCCCEEEECCCCCHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
TDITTWIDEYHKGSRFGLNGKILFKKTSKYQEIIVIENEYYGKALMLDGCWMTSLKDEK
CCHHHHHHHHCCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCEEEECCCEECCCCCHH
YYHECLVHPALSSIDEKSNVLIIGGGDGGTVRECVKYSKISKIDLVEIDEEVIKISKKFL
HHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHH
KEIAGEAWNDKRLEIHIDDGVKWVEKTKDNFYDVIFIDCSDPSEFSNLLFSHSFYKECER
HHHCCCCCCCCEEEEEECCCCHHHHHCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHH
ILAPSGILATQSESPESFKNIHINILKSLKNIFKVSETMYSFVPIYPSGIWSWTFASSED
HHCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEEECCCCC
LSLSKQNYDEVVKIEKGCEIWNLNFQNAAFKMMPNKIVKELDS
CCCCCCCHHHHHHHHCCCEEEECCCCCHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA