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The map label for this gene is miaA

Identifier: 123969377

GI number: 123969377

Start: 1575394

End: 1576293

Strand: Direct

Name: miaA

Synonym: A9601_18451

Alternate gene names: 123969377

Gene position: 1575394-1576293 (Clockwise)

Preceding gene: 123969370

Following gene: 123969378

Centisome position: 94.34

GC content: 31.0

Gene sequence:

>900_bases
ATGTCTAGAGACCTTCCTAATGTCATAATTTTGATTGGGCCTACTGCAAGTGGCAAAACAGAATTAGCTATTGAAATTGC
AGAATATTTTAAAACTCATATACATAATATCGATTCAAGGCAAATTTATAAGTCCATGGATATTGGAACAGCCAAGCCAT
CTAAAAACCAACAAAAAAAAATCAAGCATTTTTTAATAGATATTGAGGAGCCAATCCATCCAATTAATGTTAAACAATTT
CAAGGAATTGCTCAAAAATCAATAAAAAGTGAAATTAAACAAAATAATCTACCTTTTCTTGTTGGAGGTAGTGGGTTGTA
TATGAACTCGATAACAAAAGGTTTTTTTGTACCAGACGTCCCACCTCAAAATGATTTGAGAAAACAATTGGAAGAACTTG
GTCAGAAAAAATGTTGGGACTTATTAAAAAATTGTGATCCATTATCAACAAAAAAAATTAATTTTGCTGACCATATTAGA
ACAATAAGAGCTTTAGAAGTCTTTTACGTCACAGGAAAACCTTTGTCAACTTTGAAAGTGCAAAGGGCGCCTGACTGGAG
AATACTAGAGCTTGGATTAGATAGAGATAATTTAAAAGAAAGAATATTACAAAGGACAAAAAATATGTTTTCAGCGGGAA
TTATTGAAGAGACAAACTACCTCATCTCTAAATACGGATTTGATTTGCCAATATTAGAAACCATTGGATATCGAGAAGCT
AAGGATGTTTTAAAAAACCATTCAACCATTGACAAAGCAATTGAGTTAACTACGACAAAAACTATCCAATATGCAAAAAG
ACAAAAAACTTGGTTTCGTAATAAAAATAATCCTCTTTGGCTTGATAACAAAAACCTTCTAAAAGATGCAATAATTAAGA
TAGAGTCTTTTTTAAGCTAA

Upstream 100 bases:

>100_bases
TTATTTAAACTTACCACCGCAATCAGATAAAGGCTCGAAGTCAATGAAAAGTTTAATCTCTTTAATTATATAGCTAATTT
TTTTTTCTTCATAAATTCAT

Downstream 100 bases:

>100_bases
CTTTATAAAAATAGATTTTTTCATCATCCACTCAAATTATTAAATTAACTGAATGCCACCACGCCCACGTTTTGACCGCC
GAGCTCCAGTAAGAGAGCTA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSRDLPNVIILIGPTASGKTELAIEIAEYFKTHIHNIDSRQIYKSMDIGTAKPSKNQQKKIKHFLIDIEEPIHPINVKQF
QGIAQKSIKSEIKQNNLPFLVGGSGLYMNSITKGFFVPDVPPQNDLRKQLEELGQKKCWDLLKNCDPLSTKKINFADHIR
TIRALEVFYVTGKPLSTLKVQRAPDWRILELGLDRDNLKERILQRTKNMFSAGIIEETNYLISKYGFDLPILETIGYREA
KDVLKNHSTIDKAIELTTTKTIQYAKRQKTWFRNKNNPLWLDNKNLLKDAIIKIESFLS

Sequences:

>Translated_299_residues
MSRDLPNVIILIGPTASGKTELAIEIAEYFKTHIHNIDSRQIYKSMDIGTAKPSKNQQKKIKHFLIDIEEPIHPINVKQF
QGIAQKSIKSEIKQNNLPFLVGGSGLYMNSITKGFFVPDVPPQNDLRKQLEELGQKKCWDLLKNCDPLSTKKINFADHIR
TIRALEVFYVTGKPLSTLKVQRAPDWRILELGLDRDNLKERILQRTKNMFSAGIIEETNYLISKYGFDLPILETIGYREA
KDVLKNHSTIDKAIELTTTKTIQYAKRQKTWFRNKNNPLWLDNKNLLKDAIIKIESFLS
>Mature_298_residues
SRDLPNVIILIGPTASGKTELAIEIAEYFKTHIHNIDSRQIYKSMDIGTAKPSKNQQKKIKHFLIDIEEPIHPINVKQFQ
GIAQKSIKSEIKQNNLPFLVGGSGLYMNSITKGFFVPDVPPQNDLRKQLEELGQKKCWDLLKNCDPLSTKKINFADHIRT
IRALEVFYVTGKPLSTLKVQRAPDWRILELGLDRDNLKERILQRTKNMFSAGIIEETNYLISKYGFDLPILETIGYREAK
DVLKNHSTIDKAIELTTTKTIQYAKRQKTWFRNKNNPLWLDNKNLLKDAIIKIESFLS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=316, Percent_Identity=25.9493670886076, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1790613, Length=293, Percent_Identity=33.1058020477816, Blast_Score=160, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI25144712, Length=269, Percent_Identity=25.6505576208178, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324848, Length=286, Percent_Identity=26.9230769230769, Blast_Score=92, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24649830, Length=267, Percent_Identity=29.5880149812734, Blast_Score=114, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_PROMS (A2BTL7)

Other databases:

- EMBL:   CP000551
- RefSeq:   YP_001010235.1
- ProteinModelPortal:   A2BTL7
- SMR:   A2BTL7
- STRING:   A2BTL7
- GeneID:   4718582
- GenomeReviews:   CP000551_GR
- KEGG:   pmb:A9601_18451
- eggNOG:   COG0324
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- PhylomeDB:   A2BTL7
- ProtClustDB:   PRK00091
- BioCyc:   PMAR146891:A9601_18451-MONOMER
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 34433; Mature: 34302

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRDLPNVIILIGPTASGKTELAIEIAEYFKTHIHNIDSRQIYKSMDIGTAKPSKNQQKK
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHH
IKHFLIDIEEPIHPINVKQFQGIAQKSIKSEIKQNNLPFLVGGSGLYMNSITKGFFVPDV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEHHHCCCCCCCCCC
PPQNDLRKQLEELGQKKCWDLLKNCDPLSTKKINFADHIRTIRALEVFYVTGKPLSTLKV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCCEEE
QRAPDWRILELGLDRDNLKERILQRTKNMFSAGIIEETNYLISKYGFDLPILETIGYREA
ECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHCCCHHH
KDVLKNHSTIDKAIELTTTKTIQYAKRQKTWFRNKNNPLWLDNKNLLKDAIIKIESFLS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRDLPNVIILIGPTASGKTELAIEIAEYFKTHIHNIDSRQIYKSMDIGTAKPSKNQQKK
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCCHHHHH
IKHFLIDIEEPIHPINVKQFQGIAQKSIKSEIKQNNLPFLVGGSGLYMNSITKGFFVPDV
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCEEHHHCCCCCCCCCC
PPQNDLRKQLEELGQKKCWDLLKNCDPLSTKKINFADHIRTIRALEVFYVTGKPLSTLKV
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHEEEEEECCCCCCCEEE
QRAPDWRILELGLDRDNLKERILQRTKNMFSAGIIEETNYLISKYGFDLPILETIGYREA
ECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHCCCHHH
KDVLKNHSTIDKAIELTTTKTIQYAKRQKTWFRNKNNPLWLDNKNLLKDAIIKIESFLS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA