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The map label for this gene is lysS [H]

Identifier: 123969360

GI number: 123969360

Start: 1559871

End: 1561337

Strand: Reverse

Name: lysS [H]

Synonym: A9601_18281

Alternate gene names: 123969360

Gene position: 1561337-1559871 (Counterclockwise)

Preceding gene: 123969361

Following gene: 123969359

Centisome position: 93.5

GC content: 32.17

Gene sequence:

>1467_bases
GTGAATAAAGGATTTGCTTCTTACGCACAAAGCTTTAAGGTATCACATACTACCAGTTTTCTTATTCAAAAATTTGATCA
TCTAGAAAATGGTCAAGAGGAAGACTTCAGTGTTTCTATTGCTGGTAGAGTTCTGGCAAAAAGGGTAATGGGCAAAATTG
CCTTTTTCACAATAAGCGATCAAGAAGGTCAGATTCAGCTTTATCTAGATAAAAGGATTATTAATTTCAATTTAGAAAAA
CAAAAATTACTTTCTTTTGAAGATCTCAAAGAAATAGTAGATATTGGTGATTGGATAGGAGTCTATGGAACTATTAAAAA
AACTAATAAAGGTGAGCTTTCAATTAAAGTAGAAAAATGGGAAATGTTATCCAAATCATTACAACCTCTCCCAGATAAAT
GGCATGGATTGACTGATATTGAAAAAAGATATAGACAACGTTATTTAGATTTAATAGTTAATCCTCACTCTAAAAATGTA
TTTAAAACCAGAGCGAAATGTATAAGTTTTATAAGAAAATGGCTAGATAATAGAAATTTTTTAGAGATAGAGACTCCAAT
TCTGCAATCTGAAGCTGGTGGTGCTGAAGCAAGACCATTTATAACTCATCACAATACATTAGATATTCCGTTGTATTTAA
GAATAGCTACAGAATTACATTTAAAGCGAATGGTTGTTGGAGGTTTTGAGAAAGTCTATGAATTGGGAAGAATCTTCCGT
AATGAGGGGATAAGTACAAGGCATAATCCAGAATTCACCTCAGTGGAAATTTATGAAGCTTATTCTGATTATGTAGATAT
GATGAATTTAACTGAAGAATTGATTAAAGATATCGTAGCTGATGCATGTGGGTCCTTAATTATAAATTATCAAAATAAAG
AAATTGATTTTTCTAAGCCTTGGTCAAGAATATCCATGAAAGCTATTGTCAAAAAATATACAGGGATTGATTTTGATTCT
TTCAGTGGAGACTTTCTAGCAGCAAAACAAGCCGTTAAAAATATCAATGTTGATTGTTCTAATAAAGTAAATACTATGGG
AAGACTTTTAAATGAGGTCTTCGAGCAAAAAGTAGAATCAAAACTTGTAGAACCCACTTTTGTTATTGATTATCCTGTTG
AAATTTCTCCTTTAGCTAGGCCTCATCATGATAATAAACAAATAGTTCAGAGATTTGAATTATTCATTGTTGGTAGAGAA
CTGGCAAATGCGTTTAGTGAGTTGATAGATCCAGTAGATCAAAGAGAAAGAATGCAATTACAGCAATCTCTTAGAGACGA
AGGAGATCTTGAGGCTCACTGTATAGATGAAGATTTTTTAAATGCTTTAGAGATTGGCATGCCGCCTACGGGAGGATTAG
GTATAGGCATTGATAGGCTAATTATGTTAATTACTAATAGCGCATCGATTAGAGATGTAATCCCTTTCCCATTGTTAAAA
CCAGAAATAACTTCCAAAAAAAGTTAA

Upstream 100 bases:

>100_bases
GTTGAGCAATAGTCTGATAATTTTATTAAATAAAACAAATATATTTAAATAAATTTTGTCTGAAATTAAAGAAGCGCGCT
TACAAAAAGCTAGTTCACTC

Downstream 100 bases:

>100_bases
AAATTACCCTCGAATGAAGTAAAATAGTTAAATCAATCCAATACGAAATTTTTTAAATGAGTGGTGAACGTGTTGGTTTC
CGTTTCAAACACGCGGATGC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MNKGFASYAQSFKVSHTTSFLIQKFDHLENGQEEDFSVSIAGRVLAKRVMGKIAFFTISDQEGQIQLYLDKRIINFNLEK
QKLLSFEDLKEIVDIGDWIGVYGTIKKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNV
FKTRAKCISFIRKWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVVGGFEKVYELGRIFR
NEGISTRHNPEFTSVEIYEAYSDYVDMMNLTEELIKDIVADACGSLIINYQNKEIDFSKPWSRISMKAIVKKYTGIDFDS
FSGDFLAAKQAVKNINVDCSNKVNTMGRLLNEVFEQKVESKLVEPTFVIDYPVEISPLARPHHDNKQIVQRFELFIVGRE
LANAFSELIDPVDQRERMQLQQSLRDEGDLEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLITNSASIRDVIPFPLLK
PEITSKKS

Sequences:

>Translated_488_residues
MNKGFASYAQSFKVSHTTSFLIQKFDHLENGQEEDFSVSIAGRVLAKRVMGKIAFFTISDQEGQIQLYLDKRIINFNLEK
QKLLSFEDLKEIVDIGDWIGVYGTIKKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNV
FKTRAKCISFIRKWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVVGGFEKVYELGRIFR
NEGISTRHNPEFTSVEIYEAYSDYVDMMNLTEELIKDIVADACGSLIINYQNKEIDFSKPWSRISMKAIVKKYTGIDFDS
FSGDFLAAKQAVKNINVDCSNKVNTMGRLLNEVFEQKVESKLVEPTFVIDYPVEISPLARPHHDNKQIVQRFELFIVGRE
LANAFSELIDPVDQRERMQLQQSLRDEGDLEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLITNSASIRDVIPFPLLK
PEITSKKS
>Mature_488_residues
MNKGFASYAQSFKVSHTTSFLIQKFDHLENGQEEDFSVSIAGRVLAKRVMGKIAFFTISDQEGQIQLYLDKRIINFNLEK
QKLLSFEDLKEIVDIGDWIGVYGTIKKTNKGELSIKVEKWEMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNV
FKTRAKCISFIRKWLDNRNFLEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVVGGFEKVYELGRIFR
NEGISTRHNPEFTSVEIYEAYSDYVDMMNLTEELIKDIVADACGSLIINYQNKEIDFSKPWSRISMKAIVKKYTGIDFDS
FSGDFLAAKQAVKNINVDCSNKVNTMGRLLNEVFEQKVESKLVEPTFVIDYPVEISPLARPHHDNKQIVQRFELFIVGRE
LANAFSELIDPVDQRERMQLQQSLRDEGDLEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLITNSASIRDVIPFPLLK
PEITSKKS

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=494, Percent_Identity=40.8906882591093, Blast_Score=366, Evalue=1e-101,
Organism=Homo sapiens, GI194272210, Length=494, Percent_Identity=40.8906882591093, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1789256, Length=481, Percent_Identity=47.1933471933472, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1790571, Length=482, Percent_Identity=47.3029045643153, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=30.1829268292683, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI1788173, Length=251, Percent_Identity=25.8964143426295, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1787161, Length=477, Percent_Identity=20.335429769392, Blast_Score=65, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17535925, Length=498, Percent_Identity=41.566265060241, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17535927, Length=498, Percent_Identity=41.566265060241, Blast_Score=380, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71994340, Length=500, Percent_Identity=41.6, Blast_Score=380, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6320242, Length=504, Percent_Identity=41.0714285714286, Blast_Score=383, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324256, Length=482, Percent_Identity=31.9502074688797, Blast_Score=211, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=488, Percent_Identity=21.9262295081967, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640849, Length=502, Percent_Identity=41.4342629482072, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24640851, Length=502, Percent_Identity=41.4342629482072, Blast_Score=373, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24584738, Length=237, Percent_Identity=26.5822784810127, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 55992; Mature: 55992

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGFASYAQSFKVSHTTSFLIQKFDHLENGQEEDFSVSIAGRVLAKRVMGKIAFFTISD
CCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHEEEEEEEC
QEGQIQLYLDKRIINFNLEKQKLLSFEDLKEIVDIGDWIGVYGTIKKTNKGELSIKVEKW
CCCEEEEEEEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEHHH
EMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNVFKTRAKCISFIRKWLDNRNF
HHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCE
LEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVVGGFEKVYELGRIFR
EEECCCHHHCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
NEGISTRHNPEFTSVEIYEAYSDYVDMMNLTEELIKDIVADACGSLIINYQNKEIDFSKP
HCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
WSRISMKAIVKKYTGIDFDSFSGDFLAAKQAVKNINVDCSNKVNTMGRLLNEVFEQKVES
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KLVEPTFVIDYPVEISPLARPHHDNKQIVQRFELFIVGRELANAFSELIDPVDQRERMQL
HHCCCEEEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QQSLRDEGDLEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLITNSASIRDVIPFPLLK
HHHHCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCC
PEITSKKS
CCCCCCCC
>Mature Secondary Structure
MNKGFASYAQSFKVSHTTSFLIQKFDHLENGQEEDFSVSIAGRVLAKRVMGKIAFFTISD
CCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHEEEEEEEC
QEGQIQLYLDKRIINFNLEKQKLLSFEDLKEIVDIGDWIGVYGTIKKTNKGELSIKVEKW
CCCEEEEEEEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHCEEEEECCCCCEEEEEHHH
EMLSKSLQPLPDKWHGLTDIEKRYRQRYLDLIVNPHSKNVFKTRAKCISFIRKWLDNRNF
HHHHHHCCCCCHHHCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCE
LEIETPILQSEAGGAEARPFITHHNTLDIPLYLRIATELHLKRMVVGGFEKVYELGRIFR
EEECCCHHHCCCCCCCCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
NEGISTRHNPEFTSVEIYEAYSDYVDMMNLTEELIKDIVADACGSLIINYQNKEIDFSKP
HCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCC
WSRISMKAIVKKYTGIDFDSFSGDFLAAKQAVKNINVDCSNKVNTMGRLLNEVFEQKVES
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
KLVEPTFVIDYPVEISPLARPHHDNKQIVQRFELFIVGRELANAFSELIDPVDQRERMQL
HHCCCEEEEECCCEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
QQSLRDEGDLEAHCIDEDFLNALEIGMPPTGGLGIGIDRLIMLITNSASIRDVIPFPLLK
HHHHCCCCCCEEEECCHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCC
PEITSKKS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA