Click here to switch to the map view.

The map label for this gene is thiC [H]

Identifier: 123969353

GI number: 123969353

Start: 1554194

End: 1555564

Strand: Reverse

Name: thiC [H]

Synonym: A9601_18211

Alternate gene names: 123969353

Gene position: 1555564-1554194 (Counterclockwise)

Preceding gene: 123969354

Following gene: 123969349

Centisome position: 93.15

GC content: 37.42

Gene sequence:

>1371_bases
ATGAGAAGTTCTTGGATTAAGCCTCGCCTAGGGAAAGACAATGTAACTCAGATGAACTTTGCGAGAAATGGATATATCAC
TGAAGAAATGGATTTTGTTGCTAAAAAAGAGAATCTTCCTTCTTCTTTAATAATGGAAGAAGTAGCAAGAGGAAGATTAA
TTATTCCAGCTAATATTAATCATTTGAATCTTGAGCCAATGTCTATAGGTATTGCTTCAAGATGCAAAGTTAATGCCAAT
ATTGGTGCTTCCCCTAACGCAAGTGATATCAATGAAGAAGTAGAAAAGCTCAAACTTGCTGTTAAATATGGTGCTGATAC
GGTTATGGATCTTTCTACGGGAGGAGTAAATTTAGATGAAGTCCGACAAGCAATTATTCAAGAATCTCCTGTTCCCATCG
GAACTGTTCCTGTTTATCAAGCTTTAGAAAGTGTTCATGGTTCAATAGATAGACTAACAGAAGATGATTTTCTTCATATT
ATTGAAAAACATTGTCAGCAGGGCGTAGATTATCAAACTATTCATGCTGGTCTATTAATAGAGCATTTGCCAAAAGTTAA
AGGAAGAATTACTGGAATTGTCAGTAGAGGGGGAGGTATTTTAGCCCAATGGATGTTACATCATTTTAAACAAAATCCTC
TTTACACAAGGTTTGATGATATCTGTGAGATTTTCAAGAAATATGATTGTACTTTCTCTCTCGGAGATTCGCTTAGGCCT
GGATGTTTGCATGATGCTTCTGATGATGCTCAGCTAGCTGAATTGAAGACCTTAGGCGAGCTTACTCGAAGAGCATGGGA
ACATAATGTTCAAGTAATGGTTGAAGGTCCTGGTCATGTCCCTATGGATCAAATTGAGTTTAATGTGAGAAAGCAAATGG
AAGAATGTTCAGAAGCCCCTTTCTATGTACTTGGTCCATTAGTAACAGATATTTCTCCTGGTTATGACCATATATCAAGT
GCTATTGGGGCGGCAATGGCGGGGTGGTATGGAACTTCGATGCTATGTTATGTAACCCCAAAAGAACATCTAGGCCTTCC
AAATGCAGAAGATGTAAGAGAAGGATTAATTGCTTATAAAATAGCTGCTCACGCTGCTGATATAGCAAGACATAGAGCTG
GAGCTCGTGATCGAGATGATGAACTTAGTCATGCAAGGTATAACTTTGATTGGAATAAACAATTCGAACTTTCTTTAGAT
CCGGAAAGGGCAAAGCAGTACCATGATGAAACATTGCCTGAAGAGATCTTTAAAAAGGCTGAGTTTTGTTCAATGTGTGG
CCCAAAACATTGTCCAATGAATTCAAAGATTTCAGATGAATCACTAGATCAACTAAAAGATAAACTTGAAGAATGTAATA
CTTCAGTTTAG

Upstream 100 bases:

>100_bases
GCTGAGATCATACCCTTTGAACCTGAATCATTAATTTCGATGCAGGGAAGTTGAGATTTGATCAAACTTTAGTAATACCA
CTTTTAAAAATTAAGAAATT

Downstream 100 bases:

>100_bases
TTATCAATTACAAGTTATAGAATTTCCTTAGTCTTATTAACTACGTTTTCTACTGTAAATCCAAAATTTTTCATACATTC
TCCACCTGGTGCTGATGCAC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPSSLIMEEVARGRLIIPANINHLNLEPMSIGIASRCKVNAN
IGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDEVRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYKIAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLD
PERAKQYHDETLPEEIFKKAEFCSMCGPKHCPMNSKISDESLDQLKDKLEECNTSV

Sequences:

>Translated_456_residues
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPSSLIMEEVARGRLIIPANINHLNLEPMSIGIASRCKVNAN
IGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDEVRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYKIAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLD
PERAKQYHDETLPEEIFKKAEFCSMCGPKHCPMNSKISDESLDQLKDKLEECNTSV
>Mature_456_residues
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPSSLIMEEVARGRLIIPANINHLNLEPMSIGIASRCKVNAN
IGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDEVRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHI
IEKHCQQGVDYQTIHAGLLIEHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAPFYVLGPLVTDISPGYDHISS
AIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYKIAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLD
PERAKQYHDETLPEEIFKKAEFCSMCGPKHCPMNSKISDESLDQLKDKLEECNTSV

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=470, Percent_Identity=55.7446808510638, Blast_Score=516, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 51032; Mature: 51032

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPSSLIMEEVARGRLIIPANIN
CCCCCCCCCCCCCCCEEEHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HLNLEPMSIGIASRCKVNANIGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDE
EEECCHHEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
VRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
HHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEECCCCCCC
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDISPGYDHISSAIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYK
EEEECHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLDPERAKQYHDETLPEEIFKKA
HHHHHHHHHHHHCCCCCCCCHHHHCEECCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHH
EFCSMCGPKHCPMNSKISDESLDQLKDKLEECNTSV
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRSSWIKPRLGKDNVTQMNFARNGYITEEMDFVAKKENLPSSLIMEEVARGRLIIPANIN
CCCCCCCCCCCCCCCEEEHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCEEEECCCC
HLNLEPMSIGIASRCKVNANIGASPNASDINEEVEKLKLAVKYGADTVMDLSTGGVNLDE
EEECCHHEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHH
VRQAIIQESPVPIGTVPVYQALESVHGSIDRLTEDDFLHIIEKHCQQGVDYQTIHAGLLI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
EHLPKVKGRITGIVSRGGGILAQWMLHHFKQNPLYTRFDDICEIFKKYDCTFSLGDSLRP
HHHHHHHHHHEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCEECCCCCCC
GCLHDASDDAQLAELKTLGELTRRAWEHNVQVMVEGPGHVPMDQIEFNVRKQMEECSEAP
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCC
FYVLGPLVTDISPGYDHISSAIGAAMAGWYGTSMLCYVTPKEHLGLPNAEDVREGLIAYK
EEEECHHHHCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCHHHCCCCCHHHHHHHHHHHH
IAAHAADIARHRAGARDRDDELSHARYNFDWNKQFELSLDPERAKQYHDETLPEEIFKKA
HHHHHHHHHHHHCCCCCCCCHHHHCEECCCCCCEEEEEECHHHHHHHHHCCCHHHHHHHH
EFCSMCGPKHCPMNSKISDESLDQLKDKLEECNTSV
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA