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The map label for this gene is thiF [H]

Identifier: 123969308

GI number: 123969308

Start: 1508150

End: 1509295

Strand: Direct

Name: thiF [H]

Synonym: A9601_17761

Alternate gene names: 123969308

Gene position: 1508150-1509295 (Clockwise)

Preceding gene: 123969306

Following gene: 123969310

Centisome position: 90.31

GC content: 30.37

Gene sequence:

>1146_bases
ATGTCCAAAGATATAAAATTTAATTTCTTAAACTCTGATGAAGAAGAGAGATACCAAAAACATCTTACCCTTAAAGAGAT
AGGTTACGAGGGCCAATTAAATCTTAAAAACAGCTCAGTATTATGTATCGGAGCAGGTGGGCTTGGCTCTTCCGTTTTGC
TTTATCTAGCTGCAACAGGAATTGGGAGAATTGGAATAGTTGATAACGATCAAGTTGAAAAGTCTAATCTCCAGAGACAG
ATAATTCATGAAACAAATACTATTGGCAATCTTAAAATTGATTCTGCTAGGGAAAGAATTAAAAATTTCAATCCTAATTG
TGAAATATTAACCTTTTCAGAGAGAATTAATCCTAAAAATGCTCTTGAATTAATAAAGGAGTTTGATGTTATTTGTGATT
GCTCTGATAACTTTGGCACAAGATATTTAATAAATGATTCATGCCTGATATTAGATAAACCCCTAGTCTTTGGAAGTGTA
CAAGGCTTTGAAGGGCAGGTGAGTGTTTTCAATTTATATAAAAATAGTCCAAATTTAAGAGACTTACTTCCAGAATCACC
TTCAAAAAATGCTGCCCCAAGTTGTGCAGAATTCGGAGTTGTGGGTGTTTCAACAGGTTTAATAGGAATTCTTCAGGTTA
ATGAAATTATCAAAATCATTTTGAAAAAAGGTGAAATTTTAGATGGGAAGATTTTAATTTTTGATCTATTGAATATGAAT
ATGAAAAAATTACATCTAAAAAGTGATCAGTCAAATAAACGAATAAAAAACCTGTCTCAGTTTGAGGGCTTTTATAATAG
CGACGAATATTGTGAAAAAAATAATGAGATTAACATTATAAATGCTGATGAATTTAATAGTTTATACAAAGCAAAACCCA
ACAAAATTCTTTTAATTGATGTTAGAGAAAATGAAGAATTTTCTTCATTTGCAATAGAAGGATCTATCTCAATTCCCTTA
AGTCATTTGAAACAAGCATCTGACTTAAAATTTATTCAAAAAGAAAGTTTAAATAAAGAGGTTTTCACTATATGTAAATC
GGGGAAACGCTCTGAAAAAGCATCAAGAATCTTATCTAAATTCAAAATTAAGTCAAGATCTATTGAAGGCGGCATCGAAA
AGGTAAAAAAAATATTGGGCAATTAA

Upstream 100 bases:

>100_bases
CATTAATAAATTAGATCAAAAAGAAATTTTAAAAAGTCTAAAATTTTTATTTTAATTATTATATTTTCATAGTTTAGCTC
GCAAAAATTAAAGGATCTCA

Downstream 100 bases:

>100_bases
TTTTTTAAGAATAGTTAGTAATCTACACCTCTTTTTAAATCAACTCCCACATTTGCATAATGCTTATGACAAACCATTTC
AGAGTAAACATCAGCCAGTT

Product: molybdopterin biosynthesis protein

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSKDIKFNFLNSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATGIGRIGIVDNDQVEKSNLQRQ
IIHETNTIGNLKIDSARERIKNFNPNCEILTFSERINPKNALELIKEFDVICDCSDNFGTRYLINDSCLILDKPLVFGSV
QGFEGQVSVFNLYKNSPNLRDLLPESPSKNAAPSCAEFGVVGVSTGLIGILQVNEIIKIILKKGEILDGKILIFDLLNMN
MKKLHLKSDQSNKRIKNLSQFEGFYNSDEYCEKNNEINIINADEFNSLYKAKPNKILLIDVRENEEFSSFAIEGSISIPL
SHLKQASDLKFIQKESLNKEVFTICKSGKRSEKASRILSKFKIKSRSIEGGIEKVKKILGN

Sequences:

>Translated_381_residues
MSKDIKFNFLNSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATGIGRIGIVDNDQVEKSNLQRQ
IIHETNTIGNLKIDSARERIKNFNPNCEILTFSERINPKNALELIKEFDVICDCSDNFGTRYLINDSCLILDKPLVFGSV
QGFEGQVSVFNLYKNSPNLRDLLPESPSKNAAPSCAEFGVVGVSTGLIGILQVNEIIKIILKKGEILDGKILIFDLLNMN
MKKLHLKSDQSNKRIKNLSQFEGFYNSDEYCEKNNEINIINADEFNSLYKAKPNKILLIDVRENEEFSSFAIEGSISIPL
SHLKQASDLKFIQKESLNKEVFTICKSGKRSEKASRILSKFKIKSRSIEGGIEKVKKILGN
>Mature_380_residues
SKDIKFNFLNSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATGIGRIGIVDNDQVEKSNLQRQI
IHETNTIGNLKIDSARERIKNFNPNCEILTFSERINPKNALELIKEFDVICDCSDNFGTRYLINDSCLILDKPLVFGSVQ
GFEGQVSVFNLYKNSPNLRDLLPESPSKNAAPSCAEFGVVGVSTGLIGILQVNEIIKIILKKGEILDGKILIFDLLNMNM
KKLHLKSDQSNKRIKNLSQFEGFYNSDEYCEKNNEINIINADEFNSLYKAKPNKILLIDVRENEEFSSFAIEGSISIPLS
HLKQASDLKFIQKESLNKEVFTICKSGKRSEKASRILSKFKIKSRSIEGGIEKVKKILGN

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=395, Percent_Identity=34.6835443037975, Blast_Score=212, Evalue=4e-55,
Organism=Homo sapiens, GI4885649, Length=163, Percent_Identity=30.6748466257669, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI23510338, Length=164, Percent_Identity=26.219512195122, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI23510340, Length=164, Percent_Identity=26.219512195122, Blast_Score=69, Evalue=8e-12,
Organism=Homo sapiens, GI38045942, Length=150, Percent_Identity=32, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI38045944, Length=150, Percent_Identity=32, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787048, Length=237, Percent_Identity=35.4430379746835, Blast_Score=154, Evalue=1e-38,
Organism=Escherichia coli, GI87082356, Length=240, Percent_Identity=35, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1789177, Length=191, Percent_Identity=28.2722513089005, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17540406, Length=392, Percent_Identity=32.1428571428571, Blast_Score=202, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI193203301, Length=135, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321903, Length=391, Percent_Identity=33.2480818414322, Blast_Score=202, Evalue=5e-53,
Organism=Saccharomyces cerevisiae, GI6325438, Length=160, Percent_Identity=28.125, Blast_Score=70, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6320598, Length=141, Percent_Identity=30.4964539007092, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322639, Length=193, Percent_Identity=26.4248704663212, Blast_Score=67, Evalue=6e-12,
Organism=Drosophila melanogaster, GI24582879, Length=393, Percent_Identity=36.8956743002545, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24660640, Length=137, Percent_Identity=34.3065693430657, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 42725; Mature: 42593

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIKFNFLNSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATG
CCCCEEEEECCCCHHHHHHHHCHHHHCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHCC
IGRIGIVDNDQVEKSNLQRQIIHETNTIGNLKIDSARERIKNFNPNCEILTFSERINPKN
CCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCEEEEECCCCCHHH
ALELIKEFDVICDCSDNFGTRYLINDSCLILDKPLVFGSVQGFEGQVSVFNLYKNSPNLR
HHHHHHHCCEEEECCCCCCCEEEECCCEEEEECCEEEEECCCCCCCEEEEEEECCCCCHH
DLLPESPSKNAAPSCAEFGVVGVSTGLIGILQVNEIIKIILKKGEILDGKILIFDLLNMN
HHCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCC
MKKLHLKSDQSNKRIKNLSQFEGFYNSDEYCEKNNEINIINADEFNSLYKAKPNKILLID
EEEEEECCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEECHHHHHHHHCCCCEEEEEE
VRENEEFSSFAIEGSISIPLSHLKQASDLKFIQKESLNKEVFTICKSGKRSEKASRILSK
ECCCCCCCEEEEECEEECCHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
FKIKSRSIEGGIEKVKKILGN
HHHHHCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKDIKFNFLNSDEEERYQKHLTLKEIGYEGQLNLKNSSVLCIGAGGLGSSVLLYLAATG
CCCEEEEECCCCHHHHHHHHCHHHHCCCCCEEEECCCCEEEEECCCCHHHHHHHHHHCC
IGRIGIVDNDQVEKSNLQRQIIHETNTIGNLKIDSARERIKNFNPNCEILTFSERINPKN
CCEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCCCCEEEEECCCCCHHH
ALELIKEFDVICDCSDNFGTRYLINDSCLILDKPLVFGSVQGFEGQVSVFNLYKNSPNLR
HHHHHHHCCEEEECCCCCCCEEEECCCEEEEECCEEEEECCCCCCCEEEEEEECCCCCHH
DLLPESPSKNAAPSCAEFGVVGVSTGLIGILQVNEIIKIILKKGEILDGKILIFDLLNMN
HHCCCCCCCCCCCCHHHCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEHHCCC
MKKLHLKSDQSNKRIKNLSQFEGFYNSDEYCEKNNEINIINADEFNSLYKAKPNKILLID
EEEEEECCCCCHHHHHHHHHHCCCCCCCHHHCCCCCEEEEECHHHHHHHHCCCCEEEEEE
VRENEEFSSFAIEGSISIPLSHLKQASDLKFIQKESLNKEVFTICKSGKRSEKASRILSK
ECCCCCCCEEEEECEEECCHHHHCCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHH
FKIKSRSIEGGIEKVKKILGN
HHHHHCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]