Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 123969228

GI number: 123969228

Start: 1438788

End: 1440191

Strand: Direct

Name: nusA [H]

Synonym: A9601_16961

Alternate gene names: 123969228

Gene position: 1438788-1440191 (Clockwise)

Preceding gene: 123969227

Following gene: 123969229

Centisome position: 86.16

GC content: 37.32

Gene sequence:

>1404_bases
ATGGCATTAGTTATTCTCCCAGGTTTAAACAATCTCATTGAAGATATTAGTGAGGAAAAAAAGTTACCTCCTAATATCGT
GGAATTAGCCTTACGCGAAGCTTTATTAAAAGGATATGAAAAATATAGAAAAACTTTTTACATTGGAGTTAACCAAGATC
CATTTGATGAAGAATATTTTAGTAATTTTGATGTTGGACTAGATCTAGATGAAGAAGGTTACAGGATATTATCAAGTAAA
ATTATTGTAGAAGAAGTTGAGAGCGAAGATCATCAAATATCTCTAGTAGAAGTTAAGCAAGTCGCTGATGATGCTCAAAT
AGGTGACACAGTTGTTTTAGACGTTACTCCAGAAAAAGAGGATTTTGGGCGAATGGCTGCTTCAACAACAAAGCAAGTTT
TAGCCCAAAAGTTAAGAGATCAACAACGAAAAATGATCCAGGAAGAATTTGCGGATTTGGAAGATCCTGTTTTAACGGCA
AGAGTTATAAGATTTGAAAGACAATCAGTCATTATGGGAGTTAGTTCGGGTATTGGTAGACCTGAAGTTGAGGCCGAACT
TCCCAAGAGAGATCAATTACCAAATGATAATTATAGAGCAAATGCAACTTTTAAAGTATTTTTGAAAGAAGTTAGCGAAA
TTGCCAGAAAAGGGCCGCAACTTTTTGTAAGTAGAGCAAATGCTGGTTTAGTGGTTTATTTATTTGAAAATGAAGTACCG
GAAATTCAAGAAGGTACAGTGAAAATTGTTGCTGTTTCAAGAGAAGCCAACCCTCCTTCAAGAGCTGTTGGGCCAAGAAC
AAAAGTAGCTGTTGATAGTGTCGAAGAAGAAGTGGACCCTGTAGGTGCATGTATTGGAGCTAGAGGAGCAAGAATTCAAC
AAGTAGTAAATGAATTAAGGGGTGAAAAAATTGATGTTATTAAATGGTCATCTAACCCAATACAGTATATTTTAAACTCT
TTAAGTCCTGCGAAAGTAGATCAAGTAAGACTTGTAGACCCAGCAGGGCAACATGCGCACGTACTAGTTCCTCCTGATCA
ATTAAGTCTCGCAATTGGTAGAGAAGGTCAAAATGTAAGACTTGCCGCAAGATTAACTGGTTGGAAGATTGACGTTAAAA
ACTCACATGAATACGATCAGGAAGCAGAAGATGCTGCGGTCTCTGAATTAATTATTCAAAGGGAAGATGAAGAGAATCTC
CAGAGAGAAGCTGAATTAAGATTAGAAGCAGAACAAGCTGAGCGTGCTGCAGAAGATGCGAGATTAAGAGAGCTTTATCC
TCTTCCCGAAGATGAAGAAGAATATGGAGAGGAACAATACGAAGGAGTAGAATTCACAGATAATGATCCATTAGAGACTG
TTCAAGATACTGAGACATCTGCCAAAGAGGAGAAAAAACGGTGA

Upstream 100 bases:

>100_bases
GGTAAGATTAAAAAAATCCCTTTTGATGAAGTACTAAAAATTAGTCTTTGTACATTAAAAGATTAATCATTTTCAACCAT
TATTCAATTTCCCATCATAG

Downstream 100 bases:

>100_bases
TACAAGAAATCCCTGTCATGCGTTTATGCATTTCATGTAGAAAAACATATGACAGGAAAGATCTTTTAAAGATTACTAAA
GATCATAAGCAAGGTATCAT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVNQDPFDEEYFSNFDVGLDLDEEGYRILSSK
IIVEEVESEDHQISLVEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTA
RVIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVEEEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSNPIQYILNS
LSPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEENL
QREAELRLEAEQAERAAEDARLRELYPLPEDEEEYGEEQYEGVEFTDNDPLETVQDTETSAKEEKKR

Sequences:

>Translated_467_residues
MALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVNQDPFDEEYFSNFDVGLDLDEEGYRILSSK
IIVEEVESEDHQISLVEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTA
RVIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVEEEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSNPIQYILNS
LSPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEENL
QREAELRLEAEQAERAAEDARLRELYPLPEDEEEYGEEQYEGVEFTDNDPLETVQDTETSAKEEKKR
>Mature_466_residues
ALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVNQDPFDEEYFSNFDVGLDLDEEGYRILSSKI
IVEEVESEDHQISLVEVKQVADDAQIGDTVVLDVTPEKEDFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTAR
VIRFERQSVIMGVSSGIGRPEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVPE
IQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVEEEVDPVGACIGARGARIQQVVNELRGEKIDVIKWSSNPIQYILNSL
SPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVRLAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEENLQ
REAELRLEAEQAERAAEDARLRELYPLPEDEEEYGEEQYEGVEFTDNDPLETVQDTETSAKEEKKR

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=363, Percent_Identity=33.3333333333333, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 52588; Mature: 52457

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVNQDPFDEEYF
CEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHH
SNFDVGLDLDEEGYRILSSKIIVEEVESEDHQISLVEVKQVADDAQIGDTVVLDVTPEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCEEEEEECCCHH
DFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTARVIRFERQSVIMGVSSGIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHCCCCC
PEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVEEEVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIKWSSNPIQYILNSLSPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEENLQREAELRLEAEQAERAAEDA
EEEEEECEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHHHHHHHHHH
RLRELYPLPEDEEEYGEEQYEGVEFTDNDPLETVQDTETSAKEEKKR
HHHHHCCCCCCHHHHCHHHHCCCCCCCCCCCHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
ALVILPGLNNLIEDISEEKKLPPNIVELALREALLKGYEKYRKTFYIGVNQDPFDEEYF
EEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHHHH
SNFDVGLDLDEEGYRILSSKIIVEEVESEDHQISLVEVKQVADDAQIGDTVVLDVTPEKE
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCEEEEEECCCHH
DFGRMAASTTKQVLAQKLRDQQRKMIQEEFADLEDPVLTARVIRFERQSVIMGVSSGIGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEEEHHCCCCC
PEVEAELPKRDQLPNDNYRANATFKVFLKEVSEIARKGPQLFVSRANAGLVVYLFENEVP
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECCCCC
EIQEGTVKIVAVSREANPPSRAVGPRTKVAVDSVEEEVDPVGACIGARGARIQQVVNELR
CCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHC
GEKIDVIKWSSNPIQYILNSLSPAKVDQVRLVDPAGQHAHVLVPPDQLSLAIGREGQNVR
CCEEEEEEECCCHHHHHHHCCCCCCCCEEEEECCCCCCCEEEECCHHEEEEECCCCCCEE
LAARLTGWKIDVKNSHEYDQEAEDAAVSELIIQREDEENLQREAELRLEAEQAERAAEDA
EEEEEECEEEEECCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHEEHHHHHHHHHHH
RLRELYPLPEDEEEYGEEQYEGVEFTDNDPLETVQDTETSAKEEKKR
HHHHHCCCCCCHHHHCHHHHCCCCCCCCCCCHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]