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The map label for this gene is nadE [H]

Identifier: 123969180

GI number: 123969180

Start: 1392965

End: 1394662

Strand: Direct

Name: nadE [H]

Synonym: A9601_16481

Alternate gene names: 123969180

Gene position: 1392965-1394662 (Clockwise)

Preceding gene: 123969179

Following gene: 123969192

Centisome position: 83.42

GC content: 31.21

Gene sequence:

>1698_bases
ATGAAGTTTTTACTTGCCCAAATAAATCCAGTTGTTGGAGATTTGGAGGGCAATGCAAAGAAAATACTTAATATTGCCTC
AAAAGCTAGCTCAATTTCTGCTGATTTTGTTCTTACTCCTGAATTGTCATTATGGGGATATCCCGCAAATGACTTGCTTT
TAAAAAAAAATTTAATCAAAAATCAATACCAAATTCTTGATAAATTAGCTTCAGATACTAATAAAAAATATGGAAACTTA
AGTATCACAGTCGGAATAGCTGAGTTAATAAACGATTCTTTTTTTCCTAATCTTTATAATTCTATTGCGTTAGTTGAGGG
CGGTGAATGGAGAATAATTGCTCGTAAAATTATTCTTCCCACTTATGAAGTATTTGATGAGAAAAGATATTTTAGATCAG
AAGAAAAAGTTTCTGTATTGATTAAAAAAATTAAAAATCAAACTTGGCGACTTGGCTTCACTATATGTGAGGATTTATGG
GTCAACAAAAATATAGAGGGTAGAGGAATTCATAGAAAAAATCCCATTGTTGATTTAAAGAAAAAAGAAGTTGATATTTT
AGTTAATTTATCAGCCTCCCCATACACTCTGAAAAAGTTAGAGCTAAGATCCAAGGTTTCTAGTTTTGCTGCACAATATC
TTCAAGTACCGCTGATTTATGTCAACCAGATTGGAGCGAATGATAATTTAATTTTTGATGGAAATAGTTTTATTCTTGAT
AAGAATGGATCTACAATTAAGCAATTAAAATCTTTTTCAGAAGATCTCTCCAGCTGGGAAATTGAGCAAACAAAACCTGA
AAAAAAAGAATTCAAAAACTCCGAAATGTCATCAATTTTTGATGCATTAGTCCTAGGTGTTAAAGATTATGCAAAAAAAT
GTAGATTTAAAACAGCTTTAGTTGGATTAAGTGGTGGTATTGATTCAGCACTTGTCTCAGCAATTGCTACAGCAGCTTTA
GGAAGTGAAAATGTTTATTGCGTTTCAATGCCCTCTAAGTGGAGCTCATCTCATTCAAAGATTGATGCAAAAGACTTAGC
AAGAAGATTAAAGATAAGTTTCAAGAGCATTCCAATTGAAAACTTGATGAACTCTTTTGAAGAATCATTTATAAAAACCA
TAGATTTTGAAATGGCTGAAATAACAAATCAAAATATTCAGTCAAGGATAAGAGGCACACTTCTTATGGCTTTAGCAAAT
CAAGAAAAGCATTTGTTACTTTCAACAGGCAATAAATCTGAGCTGGCCGTAGGATATTGCACACTTTATGGAGATATGAA
TGGGGGATTATCGGTAATTGGGGATTTATATAAAACAAATGTTTTTAAATTATGCAATTGGCTTGATAGTGAAGATTCAA
TTAATCATAGAAAATCATATATGTTAGATACTAATGTAGATATAATTGGAAAAAATATTCGTACTAAAGCGCCAAGTGCC
GAACTAGGTCCAGATCAATTAGATACTGATTCTCTCCCACCTTATTCAATTTTAGATAATATTCTTAAAGGAATTATTGA
AGAGAAAAAAGATTTACAACAACTAGAAAAAGATGGTTATAAAAAAGATTTAATTTTAAAAATTATCTCACTCATAAAAA
AAGCGGAATTCAAAAGAAAGCAGGCTCCTCCAATCCTAAAACTAAGCAGTCAATCATTAGGAAGTGACTGGAGAGTTCCC
ATAGCAATATCTTATTGA

Upstream 100 bases:

>100_bases
GTTCTAAGGTTAGATTAAATTTTAAATGTTCTAATTTACCGTCGTCGTTAATAGATATAGTTAAGAAGAATAATTTATAT
GAATCCATCAAATTAGTTGA

Downstream 100 bases:

>100_bases
TCACTATAGGAATACTGTTGGATTTTTTTTAAAGGCCGTTTAACTGAATCAAGGTTGATACCTTTTTTGATAGCAAGAAT
TATGCCTGCAGCCTCTAAAT

Product: carbon-nitrogen hydrolase:NAD+ synthase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKFLLAQINPVVGDLEGNAKKILNIASKASSISADFVLTPELSLWGYPANDLLLKKNLIKNQYQILDKLASDTNKKYGNL
SITVGIAELINDSFFPNLYNSIALVEGGEWRIIARKIILPTYEVFDEKRYFRSEEKVSVLIKKIKNQTWRLGFTICEDLW
VNKNIEGRGIHRKNPIVDLKKKEVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSTIKQLKSFSEDLSSWEIEQTKPEKKEFKNSEMSSIFDALVLGVKDYAKKCRFKTALVGLSGGIDSALVSAIATAAL
GSENVYCVSMPSKWSSSHSKIDAKDLARRLKISFKSIPIENLMNSFEESFIKTIDFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINHRKSYMLDTNVDIIGKNIRTKAPSA
ELGPDQLDTDSLPPYSILDNILKGIIEEKKDLQQLEKDGYKKDLILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY

Sequences:

>Translated_565_residues
MKFLLAQINPVVGDLEGNAKKILNIASKASSISADFVLTPELSLWGYPANDLLLKKNLIKNQYQILDKLASDTNKKYGNL
SITVGIAELINDSFFPNLYNSIALVEGGEWRIIARKIILPTYEVFDEKRYFRSEEKVSVLIKKIKNQTWRLGFTICEDLW
VNKNIEGRGIHRKNPIVDLKKKEVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSTIKQLKSFSEDLSSWEIEQTKPEKKEFKNSEMSSIFDALVLGVKDYAKKCRFKTALVGLSGGIDSALVSAIATAAL
GSENVYCVSMPSKWSSSHSKIDAKDLARRLKISFKSIPIENLMNSFEESFIKTIDFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINHRKSYMLDTNVDIIGKNIRTKAPSA
ELGPDQLDTDSLPPYSILDNILKGIIEEKKDLQQLEKDGYKKDLILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY
>Mature_565_residues
MKFLLAQINPVVGDLEGNAKKILNIASKASSISADFVLTPELSLWGYPANDLLLKKNLIKNQYQILDKLASDTNKKYGNL
SITVGIAELINDSFFPNLYNSIALVEGGEWRIIARKIILPTYEVFDEKRYFRSEEKVSVLIKKIKNQTWRLGFTICEDLW
VNKNIEGRGIHRKNPIVDLKKKEVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSTIKQLKSFSEDLSSWEIEQTKPEKKEFKNSEMSSIFDALVLGVKDYAKKCRFKTALVGLSGGIDSALVSAIATAAL
GSENVYCVSMPSKWSSSHSKIDAKDLARRLKISFKSIPIENLMNSFEESFIKTIDFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINHRKSYMLDTNVDIIGKNIRTKAPSA
ELGPDQLDTDSLPPYSILDNILKGIIEEKKDLQQLEKDGYKKDLILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRVP
IAISY

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 63421; Mature: 63421

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLLAQINPVVGDLEGNAKKILNIASKASSISADFVLTPELSLWGYPANDLLLKKNLIK
CCEEEECCCCHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHH
NQYQILDKLASDTNKKYGNLSITVGIAELINDSFFPNLYNSIALVEGGEWRIIARKIILP
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCCHHHCCEEEEECCCEEEEEEEHHCC
TYEVFDEKRYFRSEEKVSVLIKKIKNQTWRLGFTICEDLWVNKNIEGRGIHRKNPIVDLK
CHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KKEVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCEEEEE
KNGSTIKQLKSFSEDLSSWEIEQTKPEKKEFKNSEMSSIFDALVLGVKDYAKKCRFKTAL
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHE
VGLSGGIDSALVSAIATAALGSENVYCVSMPSKWSSSHSKIDAKDLARRLKISFKSIPIE
EECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHH
NLMNSFEESFIKTIDFEMAEITNQNIQSRIRGTLLMALANQEKHLLLSTGNKSELAVGYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCEEEEEE
TLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINHRKSYMLDTNVDIIGKNIRTKAPSA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCEEEECCCEEECCCCCCCCCCC
ELGPDQLDTDSLPPYSILDNILKGIIEEKKDLQQLEKDGYKKDLILKIISLIKKAEFKRK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QAPPILKLSSQSLGSDWRVPIAISY
CCCCEEEECCCCCCCCCCEEEEEEC
>Mature Secondary Structure
MKFLLAQINPVVGDLEGNAKKILNIASKASSISADFVLTPELSLWGYPANDLLLKKNLIK
CCEEEECCCCHHCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHH
NQYQILDKLASDTNKKYGNLSITVGIAELINDSFFPNLYNSIALVEGGEWRIIARKIILP
HHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCCHHHCCEEEEECCCEEEEEEEHHCC
TYEVFDEKRYFRSEEKVSVLIKKIKNQTWRLGFTICEDLWVNKNIEGRGIHRKNPIVDLK
CHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
KKEVDILVNLSASPYTLKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
CCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCEEEEE
KNGSTIKQLKSFSEDLSSWEIEQTKPEKKEFKNSEMSSIFDALVLGVKDYAKKCRFKTAL
CCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHE
VGLSGGIDSALVSAIATAALGSENVYCVSMPSKWSSSHSKIDAKDLARRLKISFKSIPIE
EECCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCHH
NLMNSFEESFIKTIDFEMAEITNQNIQSRIRGTLLMALANQEKHLLLSTGNKSELAVGYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCEEEEECCCCCCEEEEEE
TLYGDMNGGLSVIGDLYKTNVFKLCNWLDSEDSINHRKSYMLDTNVDIIGKNIRTKAPSA
EEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCEEEECCCEEECCCCCCCCCCC
ELGPDQLDTDSLPPYSILDNILKGIIEEKKDLQQLEKDGYKKDLILKIISLIKKAEFKRK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QAPPILKLSSQSLGSDWRVPIAISY
CCCCEEEECCCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]