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The map label for this gene is murD [H]

Identifier: 123969098

GI number: 123969098

Start: 1341561

End: 1342991

Strand: Direct

Name: murD [H]

Synonym: A9601_15661

Alternate gene names: 123969098

Gene position: 1341561-1342991 (Clockwise)

Preceding gene: 123969096

Following gene: 123969099

Centisome position: 80.34

GC content: 28.86

Gene sequence:

>1431_bases
ATGATAGATAATTATTCAAGTAAAAGAAATATTAATCTTGTAATTGGATTAGGTAAATCTGGATTTTGGGCTGCCAAGTA
TTTGAGAAGCATCAATAAGAGAGTAATTGTTTGGGAAAGTAACGATGGGATAGAATTCTTAGAAAGAAAAACAGCATTAG
AAGAACTTGATGTACTAGTTTCTCTGAATAAAGAATTTGTATTTGAAGAAATTCAACCTTTTGTGAGAGAGATCGAATCT
GTTGTTGTGAGTCCATCAATACCTTATGACCATGTAACTATTGTTGAATTAAAAAAAAAGGGAATTAGAGTAATTGGAGA
AATTAATGTTGCATGGGAAATTTTAAAAGACACAAATTGGATAGGTATTACTGGCACTAATGGCAAGACTACTGTTACTC
ATTTACTAAGCCATATACTCTGCGATACTGGATTATATGCTCCTTTTGCTGGAAATATTGGTACACCTTTATGTAAATAT
GCTTACTCCAAAAAACACGAAAAAATTGATTGGGTTGTAGCTGAATTAAGCAGTTATCAAATAGAAATATCTCCAGAAGT
AAAACCTAATATTGGAATATGGACAACCTTCACAGAAGATCATCTTGAAAGACATAAAACACTTGAAAACTATTTCAATA
TAAAAAAAAGCTTGCTAGAAAAATCTGATTTTAGAATTTATAATTATGACGATAAAAACCTGAGAAATTACTACAGTTCA
CTATCAAGAGGGGTTTGGATAACAACTAGTTTAGATAAATCAAATTTTATTCATTGCGATTATTGGATAGATGATCAAGC
ATACATTGTTGAGAGAGGGAAGAAATTATTCAAACTTGAACATTTTTCTTTAAAAGGAATGCATAATCTTCAAAATCTTT
TATTGGTAATTGCAGCAGCAAGAAAAGTTGGATTATCTGGCAAGAAGATTAAAGATTCTTTATCTAATTACAAACAATTA
CCCCATAGGATGGAAACAATTTATAAAAATAATGGTCTGGAAATCATCAATGATAGTAAAGCTACAAATTTTGACTCATC
TATTGCGGGAATAAGTTCAATTGAAGGTCAAATAATAATAATTGCTGGGGGCAGATTAAAAGGCAATGAATATAGTGAGT
GGATAAAAGTTTTAAAGAAAAAAGTTAAATGTGTTTTTCTTTTTGGAGAAAGCTCAAAAGTCCTAAAAATGGCGCTTATT
AATGAAGGATTTAAAAAAAATATTTTTGAATTTTCAGAACTAAAAGAGCTTTTAAATTTTGTTTTTCATTATTTACAAAA
TAATAGAGTTGGAACATTATTATTTTCACCTTCATGCTCTAGTTTTGATCAATTTAAAAATTATGAAGAGCGTGGAGATT
ATTTCAAGAAACTAATAAGTGAAAAATTAAAGGTTAATCAATCCATTTTTTGTTCAAGTATCATTTCGTAA

Upstream 100 bases:

>100_bases
TAATTAACTTTTCTTGGGCGAATACTATAAATACTGCAGAAAGATCTCATGATTGAATTACCCCTTAAACACAATTAATA
TAAATTCAATTATAAATTTA

Downstream 100 bases:

>100_bases
TTTCCAGGTCGGTCATCACAACCGTTAACCCCTAGCAAATATTTACTTAATTAGTTAGATTTTGATTATAAATTAACCAC
TAGCAATGAGTGAATCTAAC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MIDNYSSKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESNDGIEFLERKTALEELDVLVSLNKEFVFEEIQPFVREIES
VVVSPSIPYDHVTIVELKKKGIRVIGEINVAWEILKDTNWIGITGTNGKTTVTHLLSHILCDTGLYAPFAGNIGTPLCKY
AYSKKHEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNYYSS
LSRGVWITTSLDKSNFIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAARKVGLSGKKIKDSLSNYKQL
PHRMETIYKNNGLEIINDSKATNFDSSIAGISSIEGQIIIIAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALI
NEGFKKNIFEFSELKELLNFVFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNQSIFCSSIIS

Sequences:

>Translated_476_residues
MIDNYSSKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESNDGIEFLERKTALEELDVLVSLNKEFVFEEIQPFVREIES
VVVSPSIPYDHVTIVELKKKGIRVIGEINVAWEILKDTNWIGITGTNGKTTVTHLLSHILCDTGLYAPFAGNIGTPLCKY
AYSKKHEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNYYSS
LSRGVWITTSLDKSNFIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAARKVGLSGKKIKDSLSNYKQL
PHRMETIYKNNGLEIINDSKATNFDSSIAGISSIEGQIIIIAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALI
NEGFKKNIFEFSELKELLNFVFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNQSIFCSSIIS
>Mature_476_residues
MIDNYSSKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESNDGIEFLERKTALEELDVLVSLNKEFVFEEIQPFVREIES
VVVSPSIPYDHVTIVELKKKGIRVIGEINVAWEILKDTNWIGITGTNGKTTVTHLLSHILCDTGLYAPFAGNIGTPLCKY
AYSKKHEKIDWVVAELSSYQIEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNYYSS
LSRGVWITTSLDKSNFIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAARKVGLSGKKIKDSLSNYKQL
PHRMETIYKNNGLEIINDSKATNFDSSIAGISSIEGQIIIIAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALI
NEGFKKNIFEFSELKELLNFVFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNQSIFCSSIIS

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=463, Percent_Identity=26.9978401727862, Blast_Score=154, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 54723; Mature: 54723

Theoretical pI: Translated: 9.18; Mature: 9.18

Prosite motif: PS01011 FOLYLPOLYGLU_SYNT_1 ; PS00430 TONB_DEPENDENT_REC_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDNYSSKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESNDGIEFLERKTALEELDVLV
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
SLNKEFVFEEIQPFVREIESVVVSPSIPYDHVTIVELKKKGIRVIGEINVAWEILKDTNW
HCCHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCEEEEEEHHEEEEECCCCE
IGITGTNGKTTVTHLLSHILCDTGLYAPFAGNIGTPLCKYAYSKKHEKIDWVVAELSSYQ
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHEECCCEE
IEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNYYSS
EEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
LSRGVWITTSLDKSNFIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAA
HCCCEEEEEECCCCCEEEEEEEECCCHHHHHCCHHHEEEHHHCCCCHHHHHHHHHHHHHH
RKVGLSGKKIKDSLSNYKQLPHRMETIYKNNGLEIINDSKATNFDSSIAGISSIEGQIII
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCHHCCCCEEEE
IAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALINEGFKKNIFEFSELKELLNF
EECCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNQSIFCSSIIS
HHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCC
>Mature Secondary Structure
MIDNYSSKRNINLVIGLGKSGFWAAKYLRSINKRVIVWESNDGIEFLERKTALEELDVLV
CCCCCCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHH
SLNKEFVFEEIQPFVREIESVVVSPSIPYDHVTIVELKKKGIRVIGEINVAWEILKDTNW
HCCHHHHHHHHHHHHHHHHHHEECCCCCCCCEEEEEECCCCCEEEEEEHHEEEEECCCCE
IGITGTNGKTTVTHLLSHILCDTGLYAPFAGNIGTPLCKYAYSKKHEKIDWVVAELSSYQ
EEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHEECCCEE
IEISPEVKPNIGIWTTFTEDHLERHKTLENYFNIKKSLLEKSDFRIYNYDDKNLRNYYSS
EEECCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHH
LSRGVWITTSLDKSNFIHCDYWIDDQAYIVERGKKLFKLEHFSLKGMHNLQNLLLVIAAA
HCCCEEEEEECCCCCEEEEEEEECCCHHHHHCCHHHEEEHHHCCCCHHHHHHHHHHHHHH
RKVGLSGKKIKDSLSNYKQLPHRMETIYKNNGLEIINDSKATNFDSSIAGISSIEGQIII
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHCCHHCCCCEEEE
IAGGRLKGNEYSEWIKVLKKKVKCVFLFGESSKVLKMALINEGFKKNIFEFSELKELLNF
EECCCCCCCCHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHH
VFHYLQNNRVGTLLFSPSCSSFDQFKNYEERGDYFKKLISEKLKVNQSIFCSSIIS
HHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA