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The map label for this gene is clpR [H]

Identifier: 123969044

GI number: 123969044

Start: 1296261

End: 1296923

Strand: Direct

Name: clpR [H]

Synonym: A9601_15121

Alternate gene names: 123969044

Gene position: 1296261-1296923 (Clockwise)

Preceding gene: 123969043

Following gene: 123969045

Centisome position: 77.63

GC content: 39.22

Gene sequence:

>663_bases
ATGACTGTATCTGCTCCTTATTACGGGGAAAATACCGTTATGAGGACCCCTCCCCCTGATCTCCCATCTCTTTTATTGAA
AGAGCGAATTGTTTATCTTGGTTTACCATTATTTTCAGATGATGATGCTAAAAGACAATTAGGAATGGATGTAACTGAGC
TAATCATTGCTCAACTTCTTTATTTAGAGTTTGAGGATCCAGAAAAACCAATCTATTTCTATATCAATTCAACGGGGACA
AGTTGGTACACTGGTGATGCAGTTGGTTTTGAAACAGAAGCTTTTGCTATCTGCGATACAATAAGTTACATCAAGCCCCC
AGTACACACAATATGTATCGGACAAGCAATGGGGACTGCCGCAGTTATCCTTTCATCTGGCACTAAGGGACAAAGGGCGG
CTCTTCCACATGCTTCTATTGTTTTACATCAACCTATAAGCGGAGCAAGAGGCCAAGCAACCGATATCCAAATAAGAGCT
GAGGAGGTTTTGAAAAATAAAAAATCAATGCTGGAGATTCTATCTCGTAATACTGGGAAGACTATCGAAGAACTCTCAAA
AGACTCTGACAGGATGAGTTATCTCAACCCCCAAGAAGCCCTAGATTATGGAGTTATCGATAGAATACTAACAAGTCAAA
AAGATTTACCAAATAAAATTTAA

Upstream 100 bases:

>100_bases
AAACTAATAAATAATCCTGAGTCAAACAAATCTTTTAAATCACCAGCGACACAGGGCTAAATCTAGCTAGAATCAGTTCA
ATCTTAATTTCGTGATACAA

Downstream 100 bases:

>100_bases
CTCTCACAAAACTATTTTAAAATCATGCCAATAGGAACTCCAAGCGTGCCTTACAGACTTCCAGGAAGTCAGTACGAAAG
ATGGGTTGACATATACACAA

Product: ATP-dependent Clp protease-like protein

Products: NA

Alternate protein names: Endopeptidase Clp-like [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTVSAPYYGENTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFEDPEKPIYFYINSTGT
SWYTGDAVGFETEAFAICDTISYIKPPVHTICIGQAMGTAAVILSSGTKGQRAALPHASIVLHQPISGARGQATDIQIRA
EEVLKNKKSMLEILSRNTGKTIEELSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI

Sequences:

>Translated_220_residues
MTVSAPYYGENTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFEDPEKPIYFYINSTGT
SWYTGDAVGFETEAFAICDTISYIKPPVHTICIGQAMGTAAVILSSGTKGQRAALPHASIVLHQPISGARGQATDIQIRA
EEVLKNKKSMLEILSRNTGKTIEELSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI
>Mature_219_residues
TVSAPYYGENTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLLYLEFEDPEKPIYFYINSTGTS
WYTGDAVGFETEAFAICDTISYIKPPVHTICIGQAMGTAAVILSSGTKGQRAALPHASIVLHQPISGARGQATDIQIRAE
EVLKNKKSMLEILSRNTGKTIEELSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI

Specific function: Has lost the two conserved residues (Ser and His) proposed to be part of the active site. Therefore it could be inactive [H]

COG id: COG0740

COG function: function code OU; Protease subunit of ATP-dependent Clp proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S14 family [H]

Homologues:

Organism=Homo sapiens, GI5174419, Length=192, Percent_Identity=41.1458333333333, Blast_Score=149, Evalue=2e-36,
Organism=Escherichia coli, GI1786641, Length=195, Percent_Identity=42.0512820512821, Blast_Score=163, Evalue=7e-42,
Organism=Caenorhabditis elegans, GI17538017, Length=216, Percent_Identity=38.4259259259259, Blast_Score=140, Evalue=5e-34,
Organism=Drosophila melanogaster, GI20129427, Length=191, Percent_Identity=40.8376963350785, Blast_Score=146, Evalue=8e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001907 [H]

Pfam domain/function: PF00574 CLP_protease [H]

EC number: 3.4.21.92

Molecular weight: Translated: 24341; Mature: 24210

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSAPYYGENTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLL
CCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHH
YLEFEDPEKPIYFYINSTGTSWYTGDAVGFETEAFAICDTISYIKPPVHTICIGQAMGTA
HHCCCCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHEEEHHHHHCCE
AVILSSGTKGQRAALPHASIVLHQPISGARGQATDIQIRAEEVLKNKKSMLEILSRNTGK
EEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCH
TIEELSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI
HHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVSAPYYGENTVMRTPPPDLPSLLLKERIVYLGLPLFSDDDAKRQLGMDVTELIIAQLL
CCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHH
YLEFEDPEKPIYFYINSTGTSWYTGDAVGFETEAFAICDTISYIKPPVHTICIGQAMGTA
HHCCCCCCCCEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCHHEEEHHHHHCCE
AVILSSGTKGQRAALPHASIVLHQPISGARGQATDIQIRAEEVLKNKKSMLEILSRNTGK
EEEEECCCCCCCCCCCCCEEEEECCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHCCCCH
TIEELSKDSDRMSYLNPQEALDYGVIDRILTSQKDLPNKI
HHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP imidodiphosphate; dATP degradation, [C]

Metal ions: Ca2+; Mg2+; Mn2+; Zn2+ [C]

Kcat value (1/min): 800 [C]

Specific activity: NA

Km value (mM): 1.3 {N-succinyl-Leu-Tyr} 0.21 {ATP}} [C]

Substrates: NA

Specific reaction: NA

General reaction: Hydrolysis of peptide bonds [C]

Inhibitor: Diisopropyl fluorophosphate ClpAP; Fluorosulfonyl benzoyl adenosine; Kappa-Casein; Mg2+; NEM hydrolysis; Neohydrin; Succinyl -Leu-Tyr4-methyl coumarin7-amide; Xaa-Tyr-Leu-Tyr-Trp [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]