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The map label for this gene is miaB [H]

Identifier: 123969036

GI number: 123969036

Start: 1287958

End: 1289352

Strand: Direct

Name: miaB [H]

Synonym: A9601_15041

Alternate gene names: 123969036

Gene position: 1287958-1289352 (Clockwise)

Preceding gene: 123969033

Following gene: 123969037

Centisome position: 77.13

GC content: 35.13

Gene sequence:

>1395_bases
GTGCTAACAAACACAAAACCAGACGAAAAAATTTTTCAAAAGAATTCAACTACTGGTAGTTATTGGATAACCACATTTGG
ATGCCAAATGAACAAGGCTGATTCTGAGAGAATGGCTGGTACATTGGAGAAAATGGGATATACCAGAGCAGATAATGAAT
TAAATGCGGATTTGGTCTTGTACAATACATGCACAATCAGAGATAACGCGGAGCAAAAAGTCTATAGCTTTCTAGGAAGA
CAAGCAAAAAGAAAGCACAAAACACCTAGCTTAAAACTTGTTGTTGCAGGTTGCCTTGCTCAGCAGGAGGGAGAGTCCTT
ACTAAGGAGAGTCCCAGAACTTGATCTGGTTATGGGGCCTCAACACGTAAATAATCTTGAGAATCTTCTGGGGAAAGTTG
ATTTAGGAAATCAAGTTGCTGCCACAGAAGAAAACTTCATTTCTGAAGACATAACAAGTGCCAGAAGAGAAAGCTCTATT
TGTGGCTGGGTTAATATCATTTATGGATGTAATGAAAGATGTTCATATTGTGTAGTCCCCTCTGTAAGAGGAAAAGAGCA
ATCAAGATATCCAAATGCGATAAAAAGTGAGATTCAAAAATTAGCTGGTGATAATTTTAAAGAAATTACTCTTTTGGGTC
AGAACATTGATGCATATGGTAGAGATCTTCCAGGAACAACAAAAGAGGGAAGAAAAGAAAATACTTTAACTGATCTTTTA
TATTATATTCATGATGTTAAAGGAATTCGCAGAATAAGATTTGCTACTAGTCATCCAAGATATTTTTCAAAAAGGTTGAT
TCAAGCTTGTTATGAACTTGATAAAGTCTGTGAACATTTCCATATTCCTTTCCAAAGTGGAAATGATGAGATTTTAAAGC
AAATGTCCAGAGGATATACTATCAAAAAGTATAAAAATATTATCGAGAATATAAGATCATTAATGCCAGATGCATCAATC
ACAGCTGACGCAATAGTTGCATTCCCAGGAGAAACCGAAAAACAATACCAAGATACGTTAAAGCTAATAACAGAAATTGG
CTTTGATCAAGTAAATACAGCAGCATACTCTCCAAGACCAAATACGCCTGCGGCAGTTTGGTCGAATCAACTTTCAGAAG
AGGTGAAAAAAGCTAGATTGCAAGAAATAAATGATTTGGTCGAGAAAACTGCTAGGAGCAGAAACAAAAGATACATAAAT
AATATAGAAAGCGTTTTAATTGAGGGTTTAAATCCAAAAAATTCCTCGCAAATTATGGGTAGAACTAGAACGAATAGACT
AACTTTCGTAGAGATTCCAAAAAACATTGACTTTAATTTTTCATTGGGAGATGAGATAGATGTCAGAATTAATGAAACAA
GACCTTTCTCTTTAACAGGCGAACTTTATCTATAA

Upstream 100 bases:

>100_bases
ATTCGGGGGTTCGAATCCCCTCGCTCGCCCTCAAAAAAATTGCAGCAAAATTTTTTAAATTGTAATTTTGAATTAAAGAA
TCATAAAAATTCCTAGCAAA

Downstream 100 bases:

>100_bases
TTTTTTTTCTAAATGATTGGGGGAAAGAAAAAATGTATTGGGTTAATATTTGGTGGTAATTCCAATGAACATGAAGTATC
GATATCCTCTGCAAAAACAG

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 464; Mature: 464

Protein sequence:

>464_residues
MLTNTKPDEKIFQKNSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVLYNTCTIRDNAEQKVYSFLGR
QAKRKHKTPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVAATEENFISEDITSARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLAGDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYTIKKYKNIIENIRSLMPDASI
TADAIVAFPGETEKQYQDTLKLITEIGFDQVNTAAYSPRPNTPAAVWSNQLSEEVKKARLQEINDLVEKTARSRNKRYIN
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVEIPKNIDFNFSLGDEIDVRINETRPFSLTGELYL

Sequences:

>Translated_464_residues
MLTNTKPDEKIFQKNSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVLYNTCTIRDNAEQKVYSFLGR
QAKRKHKTPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVAATEENFISEDITSARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLAGDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYTIKKYKNIIENIRSLMPDASI
TADAIVAFPGETEKQYQDTLKLITEIGFDQVNTAAYSPRPNTPAAVWSNQLSEEVKKARLQEINDLVEKTARSRNKRYIN
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVEIPKNIDFNFSLGDEIDVRINETRPFSLTGELYL
>Mature_464_residues
MLTNTKPDEKIFQKNSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVLYNTCTIRDNAEQKVYSFLGR
QAKRKHKTPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVAATEENFISEDITSARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLAGDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYTIKKYKNIIENIRSLMPDASI
TADAIVAFPGETEKQYQDTLKLITEIGFDQVNTAAYSPRPNTPAAVWSNQLSEEVKKARLQEINDLVEKTARSRNKRYIN
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVEIPKNIDFNFSLGDEIDVRINETRPFSLTGELYL

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=432, Percent_Identity=33.1018518518519, Blast_Score=238, Evalue=7e-63,
Organism=Homo sapiens, GI28872782, Length=432, Percent_Identity=33.1018518518519, Blast_Score=238, Evalue=1e-62,
Organism=Homo sapiens, GI93277076, Length=375, Percent_Identity=28.8, Blast_Score=156, Evalue=3e-38,
Organism=Escherichia coli, GI1786882, Length=445, Percent_Identity=37.0786516853933, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1787057, Length=447, Percent_Identity=26.3982102908277, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17553146, Length=437, Percent_Identity=33.4096109839817, Blast_Score=230, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=373, Percent_Identity=24.6648793565684, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356207, Length=471, Percent_Identity=30.9978768577495, Blast_Score=228, Evalue=6e-60,
Organism=Drosophila melanogaster, GI19922432, Length=462, Percent_Identity=25.3246753246753, Blast_Score=145, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52771; Mature: 52771

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTNTKPDEKIFQKNSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVL
CCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEE
YNTCTIRDNAEQKVYSFLGRQAKRKHKTPSLKLVVAGCLAQQEGESLLRRVPELDLVMGP
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
QHVNNLENLLGKVDLGNQVAATEENFISEDITSARRESSICGWVNIIYGCNERCSYCVVP
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECC
SVRGKEQSRYPNAIKSEIQKLAGDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
CCCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCHHCCCCCCCCCCCCCCHHHHHHHH
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
IKKYKNIIENIRSLMPDASITADAIVAFPGETEKQYQDTLKLITEIGFDQVNTAAYSPRP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCC
NTPAAVWSNQLSEEVKKARLQEINDLVEKTARSRNKRYINNIESVLIEGLNPKNSSQIMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
RTRTNRLTFVEIPKNIDFNFSLGDEIDVRINETRPFSLTGELYL
CCCCCCEEEEECCCCCCCEEECCCEEEEEECCCCCEEECCCEEC
>Mature Secondary Structure
MLTNTKPDEKIFQKNSTTGSYWITTFGCQMNKADSERMAGTLEKMGYTRADNELNADLVL
CCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEE
YNTCTIRDNAEQKVYSFLGRQAKRKHKTPSLKLVVAGCLAQQEGESLLRRVPELDLVMGP
EEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
QHVNNLENLLGKVDLGNQVAATEENFISEDITSARRESSICGWVNIIYGCNERCSYCVVP
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECC
SVRGKEQSRYPNAIKSEIQKLAGDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
CCCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCHHCCCCCCCCCCCCCCHHHHHHHH
YYIHDVKGIRRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYT
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
IKKYKNIIENIRSLMPDASITADAIVAFPGETEKQYQDTLKLITEIGFDQVNTAAYSPRP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHCCCCCCCCC
NTPAAVWSNQLSEEVKKARLQEINDLVEKTARSRNKRYINNIESVLIEGLNPKNSSQIMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHC
RTRTNRLTFVEIPKNIDFNFSLGDEIDVRINETRPFSLTGELYL
CCCCCCEEEEECCCCCCCEEECCCEEEEEECCCCCEEECCCEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA