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The map label for this gene is galE [C]

Identifier: 123968975

GI number: 123968975

Start: 1228290

End: 1229237

Strand: Direct

Name: galE [C]

Synonym: A9601_14431

Alternate gene names: 123968975

Gene position: 1228290-1229237 (Clockwise)

Preceding gene: 123968974

Following gene: 123968976

Centisome position: 73.56

GC content: 30.49

Gene sequence:

>948_bases
ATGAATATTTTAGTAACAGGTGGGAACGGTTATAAGGGCACCGTATTAATTCCAAAACTTTTGAAAGAAGGTCATAAAAT
TACTTCAATAGATTCAAATTTATTTGGTAACTACTTAAAACCAACTAAAAACTTAAAAATCATAAAAGAGGATATAAGAA
ATATAAAGCAAAGTCACTTCTTAGGGATAAATGCAGTTATTCATTTGGCCAATATAGCAAATGATCCTCTTGTAGAATTA
GATCCCCATTTAAGCTGGGAGGTTAATGTCTTAGCAAGTCAGCAATTGGCAGAAAAAGCTTATAGAGCAGGTGTAAAAAC
TTTTATTTATGCAAGTTCTGGAAGTGTTTATGGAATTAGTGACAAAGAACGAGTAACTGAAGATACAGAGTTACTACCTA
TCTCGGAATATAACAAAACAAAAATGGTAGCAGAAAGGATTTTTTTAAGTTACCAAGACAAAATGAAAATTTTTTGTATA
AGGCCAGCTACTGTTTGCGGTGTATCTCCAAGAATGAGGCTAGACGTTTCAGTAAACATACTAACTTTTTCTGCCTTATC
AAAAGGGCTAATAACTGTATTCGGTGGGAATCAAATACGCCCAAATATTCATATAGATGATATTTGTGATTTATACTTGT
TTTTCATAAATAAATATAGTGAACTTGAATCAGGATTTTATAATGCTGGTTTTGAAAATATCTCAATATTAGAAATTGCT
GAAGAAGTTAAGAAACTTTTACCAAATACGAAAATAGAGATAACTCCCTCAAATGACCCAAGATCTTATAGGCAATGTTC
AGATAAAATTTTAGATTTGGGGTTTATTCCTAAAAAAAGTGTGAAACATGCTATCTCTGAAATAATTGATTCCTATGAAT
CAAAAACTCTTAACACAAATGAAACATGTTTTTCTGTTAAGTGGTTAAAAAAAAGTTTACATAACTAG

Upstream 100 bases:

>100_bases
AAGGCTATACAAATTATTAGCAAGAATTAGAGAAATTTAATTTTCTTTTCGATTTAAGCAGATTCTCTTTGTGTTAATAT
GTTTTCCTAAAAACTAATCT

Downstream 100 bases:

>100_bases
AAAATGGAACCTATTTATTCATTATGTATTTGCAATTACAACATGAACGATACTCTAGAATCGAGCATAAATAGCCTACT
GGCACAAATCAATGAACGAA

Product: putative nucleoside-diphosphate sugar epimerase

Products: UDPglucoseal [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNILVTGGNGYKGTVLIPKLLKEGHKITSIDSNLFGNYLKPTKNLKIIKEDIRNIKQSHFLGINAVIHLANIANDPLVEL
DPHLSWEVNVLASQQLAEKAYRAGVKTFIYASSGSVYGISDKERVTEDTELLPISEYNKTKMVAERIFLSYQDKMKIFCI
RPATVCGVSPRMRLDVSVNILTFSALSKGLITVFGGNQIRPNIHIDDICDLYLFFINKYSELESGFYNAGFENISILEIA
EEVKKLLPNTKIEITPSNDPRSYRQCSDKILDLGFIPKKSVKHAISEIIDSYESKTLNTNETCFSVKWLKKSLHN

Sequences:

>Translated_315_residues
MNILVTGGNGYKGTVLIPKLLKEGHKITSIDSNLFGNYLKPTKNLKIIKEDIRNIKQSHFLGINAVIHLANIANDPLVEL
DPHLSWEVNVLASQQLAEKAYRAGVKTFIYASSGSVYGISDKERVTEDTELLPISEYNKTKMVAERIFLSYQDKMKIFCI
RPATVCGVSPRMRLDVSVNILTFSALSKGLITVFGGNQIRPNIHIDDICDLYLFFINKYSELESGFYNAGFENISILEIA
EEVKKLLPNTKIEITPSNDPRSYRQCSDKILDLGFIPKKSVKHAISEIIDSYESKTLNTNETCFSVKWLKKSLHN
>Mature_315_residues
MNILVTGGNGYKGTVLIPKLLKEGHKITSIDSNLFGNYLKPTKNLKIIKEDIRNIKQSHFLGINAVIHLANIANDPLVEL
DPHLSWEVNVLASQQLAEKAYRAGVKTFIYASSGSVYGISDKERVTEDTELLPISEYNKTKMVAERIFLSYQDKMKIFCI
RPATVCGVSPRMRLDVSVNILTFSALSKGLITVFGGNQIRPNIHIDDICDLYLFFINKYSELESGFYNAGFENISILEIA
EEVKKLLPNTKIEITPSNDPRSYRQCSDKILDLGFIPKKSVKHAISEIIDSYESKTLNTNETCFSVKWLKKSLHN

Specific function: Putative nucleotide sugar epimerase/dehydrogenase [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI42516563, Length=260, Percent_Identity=26.1538461538462, Blast_Score=74, Evalue=1e-13,
Organism=Homo sapiens, GI7657641, Length=327, Percent_Identity=27.5229357798165, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1786974, Length=283, Percent_Identity=26.5017667844523, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17539532, Length=313, Percent_Identity=24.6006389776358, Blast_Score=81, Evalue=6e-16,
Organism=Drosophila melanogaster, GI21356223, Length=260, Percent_Identity=26.9230769230769, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 5.1.3.2 [C]

Molecular weight: Translated: 35481; Mature: 35481

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILVTGGNGYKGTVLIPKLLKEGHKITSIDSNLFGNYLKPTKNLKIIKEDIRNIKQSHF
CEEEEECCCCCCCEEEEHHHHHCCCEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
LGINAVIHLANIANDPLVELDPHLSWEVNVLASQQLAEKAYRAGVKTFIYASSGSVYGIS
CCHHHHHEEECCCCCCCEEECCCCCEEEEHHHHHHHHHHHHHCCCEEEEEECCCCEEECC
DKERVTEDTELLPISEYNKTKMVAERIFLSYQDKMKIFCIRPATVCGVSPRMRLDVSVNI
CHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCHHEECCCCCEEEEEEEEE
LTFSALSKGLITVFGGNQIRPNIHIDDICDLYLFFINKYSELESGFYNAGFENISILEIA
EEEEHHCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
EEVKKLLPNTKIEITPSNDPRSYRQCSDKILDLGFIPKKSVKHAISEIIDSYESKTLNTN
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ETCFSVKWLKKSLHN
CCEEHHHHHHHHHCC
>Mature Secondary Structure
MNILVTGGNGYKGTVLIPKLLKEGHKITSIDSNLFGNYLKPTKNLKIIKEDIRNIKQSHF
CEEEEECCCCCCCEEEEHHHHHCCCEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHC
LGINAVIHLANIANDPLVELDPHLSWEVNVLASQQLAEKAYRAGVKTFIYASSGSVYGIS
CCHHHHHEEECCCCCCCEEECCCCCEEEEHHHHHHHHHHHHHCCCEEEEEECCCCEEECC
DKERVTEDTELLPISEYNKTKMVAERIFLSYQDKMKIFCIRPATVCGVSPRMRLDVSVNI
CHHHCCCCCCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCHHEECCCCCEEEEEEEEE
LTFSALSKGLITVFGGNQIRPNIHIDDICDLYLFFINKYSELESGFYNAGFENISILEIA
EEEEHHCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
EEVKKLLPNTKIEITPSNDPRSYRQCSDKILDLGFIPKKSVKHAISEIIDSYESKTLNTN
HHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCC
ETCFSVKWLKKSLHN
CCEEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): 57600 [C]

Specific activity: 233.3

Km value (mM): 0.256 {UDPgalactose}} 0.225 {UDPgalactose}} 0.2 {UDPgalactose}} 0.18 {UDPgalactose}} 0.16 {UDPgalactose}} 0.14 {UDPgalactose}} 0.048 {UDPgalactose}} 0.026 {UDPgalactose}} [C]

Substrates: UDPglucose [C]

Specific reaction: UDPglucose <==> UDPglucoseal [C]

General reaction: Epimerization (specificity for carbon forming a hexosulose) [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]