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The map label for this gene is rfaE [H]

Identifier: 123968974

GI number: 123968974

Start: 1226714

End: 1228219

Strand: Direct

Name: rfaE [H]

Synonym: A9601_14421

Alternate gene names: 123968974

Gene position: 1226714-1228219 (Clockwise)

Preceding gene: 123968973

Following gene: 123968975

Centisome position: 73.46

GC content: 30.54

Gene sequence:

>1506_bases
ATGAGAAATGAAATTAGTACCATAATCGAACTAAGTGAAGCTAATTTTTACTCAAATTGTATTTTAGGTTATGGGCACTT
TAATTCGATTCATCCAGGCCATATTAGATATTTAAAGCATGCCCGTGAAATTAACAAACTTTTTGTTGTGGCAATTCTTA
AGGATCTTTCCTGCGAAGGATTGATTTTCAACCAAACAGAAAGAGCAAATTCACTTGCCCAACTTGGAATTGCTGATGCA
ATAATCCTTCTACCAGATAACAGTCTCAATAAAGCTGTAAAAAAAATAAATCCAAAAGAAGTTATATTAGGCAAAGAATA
TGAAAACTCAGAATTAGAAGATATTAAAGAAACAATAAAAACGCTTCATAAAACAAAAAAATCAATATTTTTTCATGCTG
GAGAAATAAGTTATGCCAGCTCTGAATTATTGACAATATCTGAAGATAATTTAAAAAACAAACGCGAGAAGGAATTTTTA
AAAGCTTTAGAAAGACAAAATATAACCGTTGATTGTTTAAGGGAAGCAAGTAAAAACATAAAAAAATCTAATTTAATAGT
TATTGGAGATTCAATTTTAGACCAATATACTGCTTGCGAAGCTATAGGAATGAGTGCCGAGGCGCCTGTAATAGTAGTGA
AAGAATTAGATCAAAAAAACTTTTTGGGAGGAGCATCTATTGTCGCCTCTCATATTTCTTCTATAGGATCTAGTTGTAAA
TTTATTTCCATAATTGGCAATGATGGTGAAGGTTCTTGGATAAGAAATTCACTTAAAGAAAATTCAGTGATTTCTGAATT
AATAATTGATCAAAGTAGACCAACAACTTTTAAGAAAAGATATCTTGTTGAGAATCAAAAACTTTTTAGAGTTAGCCGAT
TAGATGATCATATGATTACAAAAGAAATGACAAAATTAATTCTTAATAAGGTAGAAAGTTTATCATGCGAATCTGAAGGT
ATTGTTATTTCGGATTTTGCTTATGGTCTAATTTCAGATGAACTAATAGAGGGATTAGAAAAATGTGCGAAAAAAAATAA
TCTCAAATTATATGGAGATTCTCAATCGAGTAGTCAAATTGGTGATATTTTAAGAATGAAAAATTTTACGCTTCTTTGTC
CAAATGAAAAAGAAGCCAGAATTGCTATGAATAATAAAGATATCGGCCTAGATGAGTTATGTAGAAAGTTGATGGATAAA
ACAAATTGTCAAAACCTTATAATGAAACTTGGTGCTAATGGATTTGTAGTTTATTCAAAAAGCCTAAATAATAGTCTTCA
AATTCAAGCTTTCCCAGCCCTATCAGTAAATCCAGTAGATGTTTCAGGCGCTGGCGATACATTATTATCTATAATGGCTA
CAGGTATGTGCAATGAATCTTCAATAATGAAAATAGCAGCACTTGCATGTTGTGGAGCTTCTATAGCAGTAGATACTATG
GGCAATAACCCAATAGATATTGATAAAGTTTTGAATAAGGCTATACAAATTATTAGCAAGAATTAG

Upstream 100 bases:

>100_bases
ATAGCTGAAGATACTCAGTTAATACTTTTTCATATTTGTATGCAATGGATTTCTAAAACAAGGGCCTAAGAAATATAATA
AAAAAAGTTTTCAAAACTTA

Downstream 100 bases:

>100_bases
AGAAATTTAATTTTCTTTTCGATTTAAGCAGATTCTCTTTGTGTTAATATGTTTTCCTAAAAACTAATCTATGAATATTT
TAGTAACAGGTGGGAACGGT

Product: putative ADP-heptose synthase

Products: NA

Alternate protein names: D-beta-D-heptose 7-phosphate kinase; D-beta-D-heptose 7-phosphotransferase; D-beta-D-heptose 1-phosphate adenosyltransferase [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MRNEISTIIELSEANFYSNCILGYGHFNSIHPGHIRYLKHAREINKLFVVAILKDLSCEGLIFNQTERANSLAQLGIADA
IILLPDNSLNKAVKKINPKEVILGKEYENSELEDIKETIKTLHKTKKSIFFHAGEISYASSELLTISEDNLKNKREKEFL
KALERQNITVDCLREASKNIKKSNLIVIGDSILDQYTACEAIGMSAEAPVIVVKELDQKNFLGGASIVASHISSIGSSCK
FISIIGNDGEGSWIRNSLKENSVISELIIDQSRPTTFKKRYLVENQKLFRVSRLDDHMITKEMTKLILNKVESLSCESEG
IVISDFAYGLISDELIEGLEKCAKKNNLKLYGDSQSSSQIGDILRMKNFTLLCPNEKEARIAMNNKDIGLDELCRKLMDK
TNCQNLIMKLGANGFVVYSKSLNNSLQIQAFPALSVNPVDVSGAGDTLLSIMATGMCNESSIMKIAALACCGASIAVDTM
GNNPIDIDKVLNKAIQIISKN

Sequences:

>Translated_501_residues
MRNEISTIIELSEANFYSNCILGYGHFNSIHPGHIRYLKHAREINKLFVVAILKDLSCEGLIFNQTERANSLAQLGIADA
IILLPDNSLNKAVKKINPKEVILGKEYENSELEDIKETIKTLHKTKKSIFFHAGEISYASSELLTISEDNLKNKREKEFL
KALERQNITVDCLREASKNIKKSNLIVIGDSILDQYTACEAIGMSAEAPVIVVKELDQKNFLGGASIVASHISSIGSSCK
FISIIGNDGEGSWIRNSLKENSVISELIIDQSRPTTFKKRYLVENQKLFRVSRLDDHMITKEMTKLILNKVESLSCESEG
IVISDFAYGLISDELIEGLEKCAKKNNLKLYGDSQSSSQIGDILRMKNFTLLCPNEKEARIAMNNKDIGLDELCRKLMDK
TNCQNLIMKLGANGFVVYSKSLNNSLQIQAFPALSVNPVDVSGAGDTLLSIMATGMCNESSIMKIAALACCGASIAVDTM
GNNPIDIDKVLNKAIQIISKN
>Mature_501_residues
MRNEISTIIELSEANFYSNCILGYGHFNSIHPGHIRYLKHAREINKLFVVAILKDLSCEGLIFNQTERANSLAQLGIADA
IILLPDNSLNKAVKKINPKEVILGKEYENSELEDIKETIKTLHKTKKSIFFHAGEISYASSELLTISEDNLKNKREKEFL
KALERQNITVDCLREASKNIKKSNLIVIGDSILDQYTACEAIGMSAEAPVIVVKELDQKNFLGGASIVASHISSIGSSCK
FISIIGNDGEGSWIRNSLKENSVISELIIDQSRPTTFKKRYLVENQKLFRVSRLDDHMITKEMTKLILNKVESLSCESEG
IVISDFAYGLISDELIEGLEKCAKKNNLKLYGDSQSSSQIGDILRMKNFTLLCPNEKEARIAMNNKDIGLDELCRKLMDK
TNCQNLIMKLGANGFVVYSKSLNNSLQIQAFPALSVNPVDVSGAGDTLLSIMATGMCNESSIMKIAALACCGASIAVDTM
GNNPIDIDKVLNKAIQIISKN

Specific function: Catalyzes the ADP transfer to D-glycero-D-manno-heptose 1-phosphate, yielding ADP-D,D-heptose [H]

COG id: COG2870

COG function: function code M; ADP-heptose synthase, bifunctional sugar kinase/adenylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the cytidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789432, Length=316, Percent_Identity=24.6835443037975, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023030
- InterPro:   IPR011611
- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR011913
- InterPro:   IPR011914
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2; PF00294 PfkB [H]

EC number: 2.7.-.-

Molecular weight: Translated: 55369; Mature: 55369

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNEISTIIELSEANFYSNCILGYGHFNSIHPGHIRYLKHAREINKLFVVAILKDLSCEG
CCCHHHHHHHHHCCCCHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LIFNQTERANSLAQLGIADAIILLPDNSLNKAVKKINPKEVILGKEYENSELEDIKETIK
EEECCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHH
TLHKTKKSIFFHAGEISYASSELLTISEDNLKNKREKEFLKALERQNITVDCLREASKNI
HHHHHHHHHEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCC
KKSNLIVIGDSILDQYTACEAIGMSAEAPVIVVKELDQKNFLGGASIVASHISSIGSSCK
CCCCEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCE
FISIIGNDGEGSWIRNSLKENSVISELIIDQSRPTTFKKRYLVENQKLFRVSRLDDHMIT
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
KEMTKLILNKVESLSCESEGIVISDFAYGLISDELIEGLEKCAKKNNLKLYGDSQSSSQI
HHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH
GDILRMKNFTLLCPNEKEARIAMNNKDIGLDELCRKLMDKTNCQNLIMKLGANGFVVYSK
HHHHHHCCCEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEC
SLNNSLQIQAFPALSVNPVDVSGAGDTLLSIMATGMCNESSIMKIAALACCGASIAVDTM
CCCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECC
GNNPIDIDKVLNKAIQIISKN
CCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRNEISTIIELSEANFYSNCILGYGHFNSIHPGHIRYLKHAREINKLFVVAILKDLSCEG
CCCHHHHHHHHHCCCCHHCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
LIFNQTERANSLAQLGIADAIILLPDNSLNKAVKKINPKEVILGKEYENSELEDIKETIK
EEECCCHHHHHHHHHCCCCEEEEECCCHHHHHHHHCCCHHEEECCCCCCCHHHHHHHHHH
TLHKTKKSIFFHAGEISYASSELLTISEDNLKNKREKEFLKALERQNITVDCLREASKNI
HHHHHHHHHEEECCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCC
KKSNLIVIGDSILDQYTACEAIGMSAEAPVIVVKELDQKNFLGGASIVASHISSIGSSCK
CCCCEEEEEHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCE
FISIIGNDGEGSWIRNSLKENSVISELIIDQSRPTTFKKRYLVENQKLFRVSRLDDHMIT
EEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
KEMTKLILNKVESLSCESEGIVISDFAYGLISDELIEGLEKCAKKNNLKLYGDSQSSSQI
HHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHH
GDILRMKNFTLLCPNEKEARIAMNNKDIGLDELCRKLMDKTNCQNLIMKLGANGFVVYSK
HHHHHHCCCEEECCCCCCCEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEC
SLNNSLQIQAFPALSVNPVDVSGAGDTLLSIMATGMCNESSIMKIAALACCGASIAVDTM
CCCCCEEEEEECCCCCCCEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEECC
GNNPIDIDKVLNKAIQIISKN
CCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA