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The map label for this gene is rffG [C]

Identifier: 123968952

GI number: 123968952

Start: 1191765

End: 1192823

Strand: Direct

Name: rffG [C]

Synonym: A9601_14191

Alternate gene names: 123968952

Gene position: 1191765-1192823 (Clockwise)

Preceding gene: 123968951

Following gene: 123968953

Centisome position: 71.37

GC content: 30.41

Gene sequence:

>1059_bases
ATGAATAATTATTTAATAACAGGAAACTTGGGTTATATAGGGCCTGTTTTATGTAAATTTATAAAAAAAAATGATCCTAA
TTCATTCATAACCGGATATGACAATGGTTACTTCGTTAATTGCCCCATAGCCGCAGAGGTATTGACGGAAAAACCCTTCT
TAGATTTACAAGTATATGGTGACGTTAGAGATAAAAATAAATTATCCAAATATATTGAGAAAGCAGATTTTATTGTTCAC
TTAGCAGCTATTTCAAATGATCCAATGGGTAGCGAATTTGCGAAAGTGACAAAAGAGATTAATCAAGAATCAAGTTTATT
CATAGCCAAAGAGGCTATAAATAATAATGTAAAATCATTTGTTTTTGCATCATCATGTTCAGTATATGGAACCGGATCAG
ATTCACCAAGAACTGAAAAAGATCCTGTTAAACCGCTAACTGCTTATGCAAAAAGTAAGGTAGGCACTGAGAATGATTTA
TTAGGTTTAATTAATTATAAAAACACAAAAATCACCTCACTTAGATTTTCTACCGCCTGCGGGTATAGTCCAAATTTAAG
GCTTGATCTTGTTCTTAATGATTTTGTCGCGACAGCAATTAATTCAGGCAAAATTGAAATATTAAGTGACGGGTCTCCAT
GGAGGCCACTTATAGATGTAGAAGATATGGCAAGATCTATTTTCTGGGCATGCAATAGATTATCTGGGAAACAAATGGAG
GTTATAAATGTGGGATCACAAGATTGGAACTACCAAATAAAGGATTTAGCTTTTGAAATTAAAAAATTATTAGGAGAAGA
TATTCATATAAAAATCAATAAGTCTGCTGCACCTGATAAAAGATCTTATAAAGTTTGTTTTGATAAATACTTTAATTTAA
CCCCTCAAAATTTTACACCTCAAATAAATTTGAAAAAATCTGTAATAAGAATGTTAAAGGCTCTCAAACCATTTAAAGAA
AGACTTTCAAGAGAAGATAGAAATCATTTAATTAGATTAAATGCTCTTAAATCTCTCAAACAAAACAACTTAATAGATGT
AAATTTAAATTGGAAATAA

Upstream 100 bases:

>100_bases
GGAAACTTTATATCTATATTAAATGCACCAAGTCCTGCAGCAACTTCTTCACTCAGCATTGCAGATTATGTAGTAAGATC
TCTAATTAGCTGAATTTATT

Downstream 100 bases:

>100_bases
TATATTGCAAACAACATTCTTATAGAATAAATAAAATTTTCATAAATTAATAAAGATGAATGAAATAGTTCACCTTATTT
ATAATGACAGAATAGAATCT

Product: hypothetical protein

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: UDP-Glucose 4-Epimerase; Nucleoside-Diphosphate-Sugar Epimerase; Epimerase/Dehydratase; NAD-Dependent Epimerase/Dehydratase Family Protein; DTDP-Glucose 4 6-Dehydratase; UDP-Galactose 4-Epimerase; VI Polysaccharide Biosynthesis Protein VipB/TviC; NAD Dependent Epimerase/Dehydratase Family Protein; UDP-Glucose 4-Epimerase Protein; Sugar-Nucleotide Epimerase/Dehydratase; HrEpiB; Nucleotide Sugar Epimerase; UDP-Glucuronate 5-Epimerase; UDP-N-Acetylglucosamine 4-Epimerase; NAD Dependent Epimerase; Sugar Nucleotide Epimerase/Dehydratase; Nucleoside-Diphosphate Sugar Epimerase; NAD Dependent Epimerase/Dehydratase Family; Polysaccharide Biosynthesis Protein; UDP- Or DTTP-Glucose 4-Epimerase Or 4-6-Dehydratase; Dehydratase-Like Protein; Nucleoside-Diphosphate-Sugar Epimerases; 3-Beta Hydroxysteroid Dehydrogenase/Isomerase Family; Capsular Polysaccharide Biosynthesis Protein I; DegT/DnrJ/EryC1/StrS Aminotransferase; Epimerase/Dehydratase Family Protein; Sugar Nucleotide Epimerase Dehydratase Protein; UDP-Or DTTP-Glucose 4-Epimerase Or 4-6-Dehydratase; UDP-Glucose 4 Epimerase; ADP-Glyceromanno-Heptose 6-Epimerase; DTDP-Glucose-4 6 Dehydratase; GalE-2 UDP-Glucose 4-Epimerase; Spore Coat Polysaccharide Synthesis Protein SpsJ; UDP-Glucose 4-Epimerase Related Protein; Thymidine Diphosphoglucose 4 6-Dehydratase; Nucleotide Sugar Dehydratase; NAD Dependent Epimerase/Dehydratase

Number of amino acids: Translated: 352; Mature: 352

Protein sequence:

>352_residues
MNNYLITGNLGYIGPVLCKFIKKNDPNSFITGYDNGYFVNCPIAAEVLTEKPFLDLQVYGDVRDKNKLSKYIEKADFIVH
LAAISNDPMGSEFAKVTKEINQESSLFIAKEAINNNVKSFVFASSCSVYGTGSDSPRTEKDPVKPLTAYAKSKVGTENDL
LGLINYKNTKITSLRFSTACGYSPNLRLDLVLNDFVATAINSGKIEILSDGSPWRPLIDVEDMARSIFWACNRLSGKQME
VINVGSQDWNYQIKDLAFEIKKLLGEDIHIKINKSAAPDKRSYKVCFDKYFNLTPQNFTPQINLKKSVIRMLKALKPFKE
RLSREDRNHLIRLNALKSLKQNNLIDVNLNWK

Sequences:

>Translated_352_residues
MNNYLITGNLGYIGPVLCKFIKKNDPNSFITGYDNGYFVNCPIAAEVLTEKPFLDLQVYGDVRDKNKLSKYIEKADFIVH
LAAISNDPMGSEFAKVTKEINQESSLFIAKEAINNNVKSFVFASSCSVYGTGSDSPRTEKDPVKPLTAYAKSKVGTENDL
LGLINYKNTKITSLRFSTACGYSPNLRLDLVLNDFVATAINSGKIEILSDGSPWRPLIDVEDMARSIFWACNRLSGKQME
VINVGSQDWNYQIKDLAFEIKKLLGEDIHIKINKSAAPDKRSYKVCFDKYFNLTPQNFTPQINLKKSVIRMLKALKPFKE
RLSREDRNHLIRLNALKSLKQNNLIDVNLNWK
>Mature_352_residues
MNNYLITGNLGYIGPVLCKFIKKNDPNSFITGYDNGYFVNCPIAAEVLTEKPFLDLQVYGDVRDKNKLSKYIEKADFIVH
LAAISNDPMGSEFAKVTKEINQESSLFIAKEAINNNVKSFVFASSCSVYGTGSDSPRTEKDPVKPLTAYAKSKVGTENDL
LGLINYKNTKITSLRFSTACGYSPNLRLDLVLNDFVATAINSGKIEILSDGSPWRPLIDVEDMARSIFWACNRLSGKQME
VINVGSQDWNYQIKDLAFEIKKLLGEDIHIKINKSAAPDKRSYKVCFDKYFNLTPQNFTPQINLKKSVIRMLKALKPFKE
RLSREDRNHLIRLNALKSLKQNNLIDVNLNWK

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 39789; Mature: 39789

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNYLITGNLGYIGPVLCKFIKKNDPNSFITGYDNGYFVNCPIAAEVLTEKPFLDLQVYG
CCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCEEEECCHHHHHHCCCCCEEEEEEC
DVRDKNKLSKYIEKADFIVHLAAISNDPMGSEFAKVTKEINQESSLFIAKEAINNNVKSF
CCCCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEHHHHCCCHHHE
VFASSCSVYGTGSDSPRTEKDPVKPLTAYAKSKVGTENDLLGLINYKNTKITSLRFSTAC
EEECCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEEEEC
GYSPNLRLDLVLNDFVATAINSGKIEILSDGSPWRPLIDVEDMARSIFWACNRLSGKQME
CCCCCCEEEEEEHHHHHHHCCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHCCCCCEEE
VINVGSQDWNYQIKDLAFEIKKLLGEDIHIKINKSAAPDKRSYKVCFDKYFNLTPQNFTP
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCC
QINLKKSVIRMLKALKPFKERLSREDRNHLIRLNALKSLKQNNLIDVNLNWK
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MNNYLITGNLGYIGPVLCKFIKKNDPNSFITGYDNGYFVNCPIAAEVLTEKPFLDLQVYG
CCCEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCCEEEECCHHHHHHCCCCCEEEEEEC
DVRDKNKLSKYIEKADFIVHLAAISNDPMGSEFAKVTKEINQESSLFIAKEAINNNVKSF
CCCCHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHCCCCCEEEEHHHHCCCHHHE
VFASSCSVYGTGSDSPRTEKDPVKPLTAYAKSKVGTENDLLGLINYKNTKITSLRFSTAC
EEECCCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEEECCCCEEEEEEEEEEC
GYSPNLRLDLVLNDFVATAINSGKIEILSDGSPWRPLIDVEDMARSIFWACNRLSGKQME
CCCCCCEEEEEEHHHHHHHCCCCEEEEEECCCCCCCEECHHHHHHHHHHHHHCCCCCEEE
VINVGSQDWNYQIKDLAFEIKKLLGEDIHIKINKSAAPDKRSYKVCFDKYFNLTPQNFTP
EEECCCCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCCCCC
QINLKKSVIRMLKALKPFKERLSREDRNHLIRLNALKSLKQNNLIDVNLNWK
CCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA