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The map label for this gene is gmd [H]

Identifier: 123968932

GI number: 123968932

Start: 1168547

End: 1169689

Strand: Direct

Name: gmd [H]

Synonym: A9601_13991

Alternate gene names: 123968932

Gene position: 1168547-1169689 (Clockwise)

Preceding gene: 123968931

Following gene: 123968933

Centisome position: 69.98

GC content: 32.46

Gene sequence:

>1143_bases
ATGATAAAGAATCAAAAAGTTGCTCTAATTACTGGTATTACCGGTCAAGATGGCAGCTATCTTGCGGAATTTTTATTGAA
TAAAGGTTATATAGTTCATGGAATAAAAAGAAGGTCCAGTAGTTTAAATACTGAGAGAATTGACCATATTTATCAAGATC
CGCATTTAAGTAACCCCTCATTGATATTGCATTACGGAGATTTAACTGATAGTACAAACCTTATAAGAATAATCAAATAT
GTAAAGCCAGATGAGATTTACAATCTTGGGGCTCAAAGTCATGTGAGTGTTAGCTTTGAAACTCCAGAATACACAGCTAA
CTGTGATGCGTTAGGGACCTTAAGAATATTAGAAGCAATTAGATTATTAAAACTTGAAAAAATTACTAAATTTTATCAAG
CTAGTACAAGCGAATTATATGGTTTAGTGCAAGAAACCCCTCAAACTGAGAAAACACCTTTTTACCCTAGAAGTCCTTAT
GGAGTAGCTAAACTTTACTCATATTGGATAACTGTAAATTACAGAGAATCCTATGGAATATTTGCTTGCAATGGAATTCT
TTTTAATCATGAAAGTCCAAGAAGAGGAGAGACATTTGTTACAAGAAAAATAACAAGAGGATTATCTCGAATAGATCAGG
GCTTAGAAAATTGTATTTATCTTGGTAATCTTGATGCATTAAGAGATTGGGGACATGCTGTTGATTATGTAGAAATGCAG
TGGAAGATGTTACAGCAAAAAGACCCAAAAGATTATGTAATTTCAACAGGCAGGCAGGAAAGTGTAAGAAGATTTATTCA
TTTAGCAGCTTTGGCATTGGGGTGGAAAACAAATGCAGAGAATAATGCTATTGTTTGGGAGGGTTCAGGATTAGATGAAG
TAGGAAGAAGAGTTGATACAAATGAAATTATTATAAGGATTGATCCAAAATATTTCAGACCATCAGAAGTTGAAACACTC
CTTGGAGATTCTTCAAAAGCATATGAAGAACTTAATTGGAAACCAAAAATAAGTTTAGAAGAGCTTATAAAAGAAATGAT
AAATGCAGATATTGAGTTAGCAAAAAAAGAAGCTTTTTTATTTAGAAAAGGATTTAACATCCCTAAATCAATTGAAAACC
CTCCGAGCATTTTTGAAAAATAA

Upstream 100 bases:

>100_bases
TAATTAGTAAATTTTTTTACTCACTTTTAAATAGAATAACTTAGAAGTAATTTATTTATGTATCCCAAAATTTATATGAC
AAGTTTCAATAGGATTTAAA

Downstream 100 bases:

>100_bases
ATATACATTATAATGAAAAAATTAATTACCAAAGAAGATAAAATATTTATTGCTGGTCATAAAGGAATGGTTGGTAGGTC
AATTAAAAAAAATTTAATTT

Product: gdpmannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MIKNQKVALITGITGQDGSYLAEFLLNKGYIVHGIKRRSSSLNTERIDHIYQDPHLSNPSLILHYGDLTDSTNLIRIIKY
VKPDEIYNLGAQSHVSVSFETPEYTANCDALGTLRILEAIRLLKLEKITKFYQASTSELYGLVQETPQTEKTPFYPRSPY
GVAKLYSYWITVNYRESYGIFACNGILFNHESPRRGETFVTRKITRGLSRIDQGLENCIYLGNLDALRDWGHAVDYVEMQ
WKMLQQKDPKDYVISTGRQESVRRFIHLAALALGWKTNAENNAIVWEGSGLDEVGRRVDTNEIIIRIDPKYFRPSEVETL
LGDSSKAYEELNWKPKISLEELIKEMINADIELAKKEAFLFRKGFNIPKSIENPPSIFEK

Sequences:

>Translated_380_residues
MIKNQKVALITGITGQDGSYLAEFLLNKGYIVHGIKRRSSSLNTERIDHIYQDPHLSNPSLILHYGDLTDSTNLIRIIKY
VKPDEIYNLGAQSHVSVSFETPEYTANCDALGTLRILEAIRLLKLEKITKFYQASTSELYGLVQETPQTEKTPFYPRSPY
GVAKLYSYWITVNYRESYGIFACNGILFNHESPRRGETFVTRKITRGLSRIDQGLENCIYLGNLDALRDWGHAVDYVEMQ
WKMLQQKDPKDYVISTGRQESVRRFIHLAALALGWKTNAENNAIVWEGSGLDEVGRRVDTNEIIIRIDPKYFRPSEVETL
LGDSSKAYEELNWKPKISLEELIKEMINADIELAKKEAFLFRKGFNIPKSIENPPSIFEK
>Mature_380_residues
MIKNQKVALITGITGQDGSYLAEFLLNKGYIVHGIKRRSSSLNTERIDHIYQDPHLSNPSLILHYGDLTDSTNLIRIIKY
VKPDEIYNLGAQSHVSVSFETPEYTANCDALGTLRILEAIRLLKLEKITKFYQASTSELYGLVQETPQTEKTPFYPRSPY
GVAKLYSYWITVNYRESYGIFACNGILFNHESPRRGETFVTRKITRGLSRIDQGLENCIYLGNLDALRDWGHAVDYVEMQ
WKMLQQKDPKDYVISTGRQESVRRFIHLAALALGWKTNAENNAIVWEGSGLDEVGRRVDTNEIIIRIDPKYFRPSEVETL
LGDSSKAYEELNWKPKISLEELIKEMINADIELAKKEAFLFRKGFNIPKSIENPPSIFEK

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=357, Percent_Identity=60.2240896358543, Blast_Score=446, Evalue=1e-125,
Organism=Escherichia coli, GI1788366, Length=373, Percent_Identity=67.2922252010724, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI48994969, Length=239, Percent_Identity=27.1966527196653, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1788353, Length=258, Percent_Identity=24.8062015503876, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17507723, Length=358, Percent_Identity=60.6145251396648, Blast_Score=453, Evalue=1e-128,
Organism=Caenorhabditis elegans, GI133901788, Length=356, Percent_Identity=60.1123595505618, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17539424, Length=355, Percent_Identity=60.2816901408451, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI17539422, Length=356, Percent_Identity=60.1123595505618, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI133901786, Length=356, Percent_Identity=60.1123595505618, Blast_Score=452, Evalue=1e-127,
Organism=Caenorhabditis elegans, GI133901790, Length=356, Percent_Identity=60.1123595505618, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24158427, Length=352, Percent_Identity=60.7954545454545, Blast_Score=447, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 43584; Mature: 43584

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKNQKVALITGITGQDGSYLAEFLLNKGYIVHGIKRRSSSLNTERIDHIYQDPHLSNPS
CCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHHHCCCCCCCCE
LILHYGDLTDSTNLIRIIKYVKPDEIYNLGAQSHVSVSFETPEYTANCDALGTLRILEAI
EEEEECCCCCCCHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
RLLKLEKITKFYQASTSELYGLVQETPQTEKTPFYPRSPYGVAKLYSYWITVNYRESYGI
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEEEECCCCE
FACNGILFNHESPRRGETFVTRKITRGLSRIDQGLENCIYLGNLDALRDWGHAVDYVEMQ
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHH
WKMLQQKDPKDYVISTGRQESVRRFIHLAALALGWKTNAENNAIVWEGSGLDEVGRRVDT
HHHHHCCCCHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCCC
NEIIIRIDPKYFRPSEVETLLGDSSKAYEELNWKPKISLEELIKEMINADIELAKKEAFL
CEEEEEECCCCCCCCHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
FRKGFNIPKSIENPPSIFEK
HHHCCCCCCCCCCCHHHHCC
>Mature Secondary Structure
MIKNQKVALITGITGQDGSYLAEFLLNKGYIVHGIKRRSSSLNTERIDHIYQDPHLSNPS
CCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEHHHHCCCCCHHHHHHHHCCCCCCCCE
LILHYGDLTDSTNLIRIIKYVKPDEIYNLGAQSHVSVSFETPEYTANCDALGTLRILEAI
EEEEECCCCCCCHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHH
RLLKLEKITKFYQASTSELYGLVQETPQTEKTPFYPRSPYGVAKLYSYWITVNYRESYGI
HHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHEEEEEEEEEECCCCE
FACNGILFNHESPRRGETFVTRKITRGLSRIDQGLENCIYLGNLDALRDWGHAVDYVEMQ
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHHH
WKMLQQKDPKDYVISTGRQESVRRFIHLAALALGWKTNAENNAIVWEGSGLDEVGRRVDT
HHHHHCCCCHHHEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHHCCCCCC
NEIIIRIDPKYFRPSEVETLLGDSSKAYEELNWKPKISLEELIKEMINADIELAKKEAFL
CEEEEEECCCCCCCCHHHHHHCCCHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHH
FRKGFNIPKSIENPPSIFEK
HHHCCCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]