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The map label for this gene is pnp [H]

Identifier: 123968917

GI number: 123968917

Start: 1150972

End: 1153137

Strand: Direct

Name: pnp [H]

Synonym: A9601_13841

Alternate gene names: 123968917

Gene position: 1150972-1153137 (Clockwise)

Preceding gene: 123968916

Following gene: 123968919

Centisome position: 68.93

GC content: 38.83

Gene sequence:

>2166_bases
GTGGAAGGACAAAATAAGTCGATCACGTTTGACGGACGAGAGATACGACTAACTACAGGACTATACGCTCCTCAAGCAAG
TGGATCAGTAATGATTGAGTGTGGTGATACCTCACTATTAGTTACAGCTACAAAAACTGCCAAAAAAGAACCATCAGACT
TTCTCCCTCTGATATGCGACTATGAAGAAAAACTTTTTGCTGCAGGGAGAATTCCTGGTGGTTTTATGAGGAGAGAAGGT
CGCCCACCAGAAAGAGCGACTTTAATTGCAAGATTAATTGATAGGCCAATGAGGCCACTTTTCCCCACATGGATGGGGGA
TGAGATTCAAATAGTCGCATCATGCCTTTCTCTAGATGAAAGAGTTCCAGCAGATGTTTTGGCTGTTACCGGGGCTTCAA
TAGCAACTTTAGTTGGAGAGATTCCATTTTATGGGCCAATGGCTGCTGTAAGAGTTGGGCTGATAGGCGATGATTTCATC
TTAAATCCTAGTTATAGAGAGATTGAAAAGGGAGATTTAGACATTGTTGTTGCAGGATCACCAGACGGTATCGTCATGAT
TGAAGCAGGTGCAAATCAATTGTCAGAGCAAGATACAATCGAAGCTATAGATTTCGGATATGAGGCTGTTACTGAACTTA
TTAAATCGCAAGAAGATTTACTACAAGATTTAGGAATAAAACAGATTAAGCCATCTGAACCTGAAGAAGATAAAACATTA
CCCTCTTATTTAGAGAAAAATTGTACAAAACCTATTGAGTTAGTTTTAAAGAAATTTGACCTTTCAAAGGAAGAAAGAGA
TCTTGAACTCGAAAAAATTAAAACTGAAACTCAAAGCAAAATTGATTCACTTAAAGATGACAATCAAGTAAAAGTTCTAA
CCTCAGAAAATGAAAAATTGCTTCATTCCGACTTTAAAAAATTAACAAAAAAATTAATGAGGTCGCAAATCATAAATGAT
GGGAAAAGAGTTGATGGAAGGGATCTCGATGAAGTTAGAAAAATATCAGCCTCTGCAGGAATTCTTCCAAAAAGAGTTCA
TGGCTCTGCACTTTTTCAAAGAGGCCTAACTCAAGTCTTATCTACTACTACTCTTGGCACGCCAAGCGATGCGCAAGAGA
TGGATGATTTAAATCCAAGTACTGAAAAAACATATTTGCATCACTATAATTTTCCTCCTTATTCAGTGGGAGAAACAAGA
CCAATGAGAACTCCAGGCAGAAGAGAAATTGGTCATGGAGCATTAGCTGAAAGAGCAATAATACCTGTGCTGCCTGGTAA
AGAAACATTTCCTTATGTTTTAAGGGTAGTAAGTGAAGTTTTAAGTTCTAACGGATCAACCTCAATGGGTTCTGTATGCG
GCAGCACTCTTTCGTTACTAGATGCAGGAGTCCCTTTAAAGGCTCCTGTAAGTGGGACTGCTATGGGCTTAATCAAGGAA
GGTAAAGAAGTCCGAATTCTCACAGATATTCAAGGAATTGAAGACTTTCTTGGAGACATGGACTTTAAAGTTGCAGGTAC
TGACAAGGGTATAACCGCTTTACAAATGGATATGAAAATTACAGGCTTACCAGTATCTATTATTTCTGATGCAATTAAAA
AAGCTCGTCCTGCAAGAATACATATTTTAGAAAAGATGCAAGGGGCAATAGATAAGCCTCAAGAATCTCTTTCTCCTCAT
GCCCCCCGACTTTTAAGCTTTAGAATTGACCCTGAGCTTATAGGAACAGTTATTGGACCCGGCGGTAGAACTATCAAAGG
AATCACTGAAAGAACAAATACAAAAATAGATATAGAAGATGGTGGCATTGTAACTATTGCTTCTCATGATGGAGCTGCGG
CAGAGGAAGCTCAAAAGATAATAGAAGGATTAACACGAAAGGTTCATGAAGGTGAGATTTTCTCCGGAGTCGTAACACGA
ATAATACCAATAGGTGCTTTTGTAGAAATATTGCCAGGCAAAGAAGGGATGGTTCACATTTCTCAATTATCTGAAGCAAG
GGTGGAGAGAGTCGAAGATGTAGTAAGGCAAGGTGATGAAGTAACCGTAAGAGTTAGAGAGATTGATAGTAGAGGGAGAA
TTAATCTTACTTTGAGAGGAGTAGGCCAAAATAATGGAATGTCTTATCCAGAACCAACTCCTACTCCAGTTGCTCCGCTT
AATTAA

Upstream 100 bases:

>100_bases
AGGACATTTTTAAAAATTTTTATAGCTTATCTAAATAATTTACTCAATATGTCCCTATTAAATGTAAGAATAAATTATGT
ATAGATTTATAATTTAAAAA

Downstream 100 bases:

>100_bases
AACTAAAGAGGATAGATTCCATAATCCTCAATAATTTTCTTTATTTCTAAGCAAATATTTCCGTGAGTTTTCTTGTCATT
TGTAGCAATTATAATTCCTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase [H]

Number of amino acids: Translated: 721; Mature: 721

Protein sequence:

>721_residues
MEGQNKSITFDGREIRLTTGLYAPQASGSVMIECGDTSLLVTATKTAKKEPSDFLPLICDYEEKLFAAGRIPGGFMRREG
RPPERATLIARLIDRPMRPLFPTWMGDEIQIVASCLSLDERVPADVLAVTGASIATLVGEIPFYGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSPDGIVMIEAGANQLSEQDTIEAIDFGYEAVTELIKSQEDLLQDLGIKQIKPSEPEEDKTL
PSYLEKNCTKPIELVLKKFDLSKEERDLELEKIKTETQSKIDSLKDDNQVKVLTSENEKLLHSDFKKLTKKLMRSQIIND
GKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARIHILEKMQGAIDKPQESLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFSGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNNGMSYPEPTPTPVAPL
N

Sequences:

>Translated_721_residues
MEGQNKSITFDGREIRLTTGLYAPQASGSVMIECGDTSLLVTATKTAKKEPSDFLPLICDYEEKLFAAGRIPGGFMRREG
RPPERATLIARLIDRPMRPLFPTWMGDEIQIVASCLSLDERVPADVLAVTGASIATLVGEIPFYGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSPDGIVMIEAGANQLSEQDTIEAIDFGYEAVTELIKSQEDLLQDLGIKQIKPSEPEEDKTL
PSYLEKNCTKPIELVLKKFDLSKEERDLELEKIKTETQSKIDSLKDDNQVKVLTSENEKLLHSDFKKLTKKLMRSQIIND
GKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARIHILEKMQGAIDKPQESLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFSGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNNGMSYPEPTPTPVAPL
N
>Mature_721_residues
MEGQNKSITFDGREIRLTTGLYAPQASGSVMIECGDTSLLVTATKTAKKEPSDFLPLICDYEEKLFAAGRIPGGFMRREG
RPPERATLIARLIDRPMRPLFPTWMGDEIQIVASCLSLDERVPADVLAVTGASIATLVGEIPFYGPMAAVRVGLIGDDFI
LNPSYREIEKGDLDIVVAGSPDGIVMIEAGANQLSEQDTIEAIDFGYEAVTELIKSQEDLLQDLGIKQIKPSEPEEDKTL
PSYLEKNCTKPIELVLKKFDLSKEERDLELEKIKTETQSKIDSLKDDNQVKVLTSENEKLLHSDFKKLTKKLMRSQIIND
GKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVLSTTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETR
PMRTPGRREIGHGALAERAIIPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARIHILEKMQGAIDKPQESLSPH
APRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIEDGGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFSGVVTR
IIPIGAFVEILPGKEGMVHISQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNNGMSYPEPTPTPVAPL
N

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction [H]

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI188528628, Length=741, Percent_Identity=35.357624831309, Blast_Score=436, Evalue=1e-122,
Organism=Escherichia coli, GI145693187, Length=699, Percent_Identity=45.3505007153076, Blast_Score=596, Evalue=1e-171,
Organism=Caenorhabditis elegans, GI115534063, Length=677, Percent_Identity=33.97341211226, Blast_Score=350, Evalue=2e-96,
Organism=Drosophila melanogaster, GI281362905, Length=681, Percent_Identity=37.0044052863436, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651641, Length=681, Percent_Identity=37.0044052863436, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI24651643, Length=681, Percent_Identity=37.0044052863436, Blast_Score=425, Evalue=1e-119,
Organism=Drosophila melanogaster, GI161079377, Length=625, Percent_Identity=36.96, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967 [H]

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1 [H]

EC number: =2.7.7.8 [H]

Molecular weight: Translated: 78606; Mature: 78606

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGQNKSITFDGREIRLTTGLYAPQASGSVMIECGDTSLLVTATKTAKKEPSDFLPLICD
CCCCCCCEEECCCEEEEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCEEEC
YEEKLFAAGRIPGGFMRREGRPPERATLIARLIDRPMRPLFPTWMGDEIQIVASCLSLDE
CHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
RVPADVLAVTGASIATLVGEIPFYGPMAAVRVGLIGDDFILNPSYREIEKGDLDIVVAGS
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHHHCCCCEEEEEECC
PDGIVMIEAGANQLSEQDTIEAIDFGYEAVTELIKSQEDLLQDLGIKQIKPSEPEEDKTL
CCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PSYLEKNCTKPIELVLKKFDLSKEERDLELEKIKTETQSKIDSLKDDNQVKVLTSENEKL
HHHHHHCCCCHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
LHSDFKKLTKKLMRSQIINDGKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
STTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETRPMRTPGRREIGHGALAERAI
HHCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCE
IPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARI
CCEEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCHHH
HILEKMQGAIDKPQESLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHCCCCHHHCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFSGVVTRIIPIGAFVEILPGKEGMVHI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCEEEH
SQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNNGMSYPEPTPTPVAPL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
N
C
>Mature Secondary Structure
MEGQNKSITFDGREIRLTTGLYAPQASGSVMIECGDTSLLVTATKTAKKEPSDFLPLICD
CCCCCCCEEECCCEEEEEECCCCCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCEEEC
YEEKLFAAGRIPGGFMRREGRPPERATLIARLIDRPMRPLFPTWMGDEIQIVASCLSLDE
CHHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHH
RVPADVLAVTGASIATLVGEIPFYGPMAAVRVGLIGDDFILNPSYREIEKGDLDIVVAGS
CCCHHHHHHCCHHHHHHHHCCCCCCCHHHHEEEEECCCEEECCCHHHHCCCCEEEEEECC
PDGIVMIEAGANQLSEQDTIEAIDFGYEAVTELIKSQEDLLQDLGIKQIKPSEPEEDKTL
CCCEEEEECCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
PSYLEKNCTKPIELVLKKFDLSKEERDLELEKIKTETQSKIDSLKDDNQVKVLTSENEKL
HHHHHHCCCCHHHHHHHHHCCCCCHHCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHH
LHSDFKKLTKKLMRSQIINDGKRVDGRDLDEVRKISASAGILPKRVHGSALFQRGLTQVL
HHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHH
STTTLGTPSDAQEMDDLNPSTEKTYLHHYNFPPYSVGETRPMRTPGRREIGHGALAERAI
HHCCCCCCCCHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHCCCCHHHHCCE
IPVLPGKETFPYVLRVVSEVLSSNGSTSMGSVCGSTLSLLDAGVPLKAPVSGTAMGLIKE
EECCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHC
GKEVRILTDIQGIEDFLGDMDFKVAGTDKGITALQMDMKITGLPVSIISDAIKKARPARI
CCEEEEEEHHHHHHHHHCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCHHH
HILEKMQGAIDKPQESLSPHAPRLLSFRIDPELIGTVIGPGGRTIKGITERTNTKIDIED
HHHHHHHHHCCCCHHHCCCCCCCEEEEEECHHHHHEEECCCCCEEECHHHCCCCEEEECC
GGIVTIASHDGAAAEEAQKIIEGLTRKVHEGEIFSGVVTRIIPIGAFVEILPGKEGMVHI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEECCCCCCEEEH
SQLSEARVERVEDVVRQGDEVTVRVREIDSRGRINLTLRGVGQNNGMSYPEPTPTPVAPL
HHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCEEEEEEEECCCCCCCCCCCCCCCCCCCC
N
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA