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The map label for this gene is dus1 [H]

Identifier: 123968631

GI number: 123968631

Start: 938171

End: 939178

Strand: Direct

Name: dus1 [H]

Synonym: A9601_10981

Alternate gene names: 123968631

Gene position: 938171-939178 (Clockwise)

Preceding gene: 123968628

Following gene: 123968632

Centisome position: 56.18

GC content: 32.94

Gene sequence:

>1008_bases
ATGTCTTCAAATATAAGGCTAAAAGGAAGGGGAGTTAACAGAAAAATTACGAGTAAGGTAATGCTATCGCCATTAGCAGG
AGTTACGGATAACATTTTTAGACGACTTGTACGTAAATGGGCTCCAAACTCTTTACTTTTTACAGAAATGATAAATGCCA
CAAGTCTTAAAAAAGGATATGGCACACAAAAAATCAATCAAATAGATTTAGAAGAAGGTCCAATTGGAGTACAAATATTT
GATAATAGGCCATATGCTGTTTCTGAAGCTGCAAAACAAGCTGAGGACTCTGGAGCTTTCTTAATCGATATAAATATGGG
ATGTCCAGTAAAAAAAATTGCAAAGAAAGGTGGAGGCAGTGCCTTAATTAAAGACCGAAAACTTGCTATAGAATTAGTCA
AAAATGTTGTAAAAGCTGTTAGGGTTCCTGTAACAGTAAAAACACGACTCGGATGGGATAGTAAAGAAGAAAATATAGAG
GATTTCTTATTTAAACTTCAAGATGCGGGAGCAACCATGATCACACTTCATGGAAGAACTAGAAAACAGGGTTTTTCAGG
CAAGTCAGATTGGGAAATGATCGGGAGACTTAAAAAGTTGTTGGAAATTCCAGTAATTGCTAATGGAGATATCAAAAATC
CAGATGACGCTCTTAATTGTTTGAAAAAAACAAAAGCTGATGGTGTAATGATTGGACGAGGAATTTTAGGATCCCCATGG
AAAATAGGAGAAATAGATTATGCTCTTAGAGAAAATAAAAATTTTAAAGAACCAAACACAGAAGAAAAACTATATTTAAT
TATTGAGCATCTTGATGAATTAATAAAAGAAAAAGGAGATCACGGTTTGCTAATCGCAAGGAAACATATCTCATGGACAT
GCAAAGACTTTAAAGGGGCATCAAATTTGAGAAATAACTTGGTTAGAGCTGTTGATAAAAATGAAGTTAAAAATTTAATA
AATAAAATGATTCAAACTTTGAATAATGAAAAAAATAGATTAGCTTAA

Upstream 100 bases:

>100_bases
GTAAAAGCACTTAGTATAAATTAAGAAATAAATAGAAAACGTAAGATCATCTCCCTATCTGAATTTATAATAAATAAAGA
TTAAATAAATTTAAAATTAT

Downstream 100 bases:

>100_bases
AACAAATTTATTTTTTAAATGAAAATTATTAGTAAAGAAACAAGTAAAAGAGTTTGTGATCACATGAATAATGATCACAT
AGATTCAGTACACAAATATC

Product: nitrogen regulation protein NifR3 family-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSSNIRLKGRGVNRKITSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGYGTQKINQIDLEEGPIGVQIF
DNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIE
DFLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTKADGVMIGRGILGSPW
KIGEIDYALRENKNFKEPNTEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLI
NKMIQTLNNEKNRLA

Sequences:

>Translated_335_residues
MSSNIRLKGRGVNRKITSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGYGTQKINQIDLEEGPIGVQIF
DNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIE
DFLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTKADGVMIGRGILGSPW
KIGEIDYALRENKNFKEPNTEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLI
NKMIQTLNNEKNRLA
>Mature_334_residues
SSNIRLKGRGVNRKITSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGYGTQKINQIDLEEGPIGVQIFD
NRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIED
FLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTKADGVMIGRGILGSPWK
IGEIDYALRENKNFKEPNTEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLIN
KMIQTLNNEKNRLA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=236, Percent_Identity=31.7796610169492, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=31.7796610169492, Blast_Score=110, Evalue=1e-24,
Organism=Homo sapiens, GI40807366, Length=228, Percent_Identity=31.140350877193, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI31742496, Length=226, Percent_Identity=34.070796460177, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI8923374, Length=142, Percent_Identity=33.0985915492958, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789660, Length=303, Percent_Identity=34.6534653465347, Blast_Score=175, Evalue=4e-45,
Organism=Escherichia coli, GI1788462, Length=328, Percent_Identity=32.0121951219512, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI145693211, Length=307, Percent_Identity=26.3843648208469, Blast_Score=98, Evalue=6e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=264, Percent_Identity=33.3333333333333, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25144369, Length=212, Percent_Identity=31.6037735849057, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=237, Percent_Identity=29.1139240506329, Blast_Score=87, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=244, Percent_Identity=32.7868852459016, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=304, Percent_Identity=25.3289473684211, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=29.646017699115, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=249, Percent_Identity=33.7349397590361, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24580595, Length=227, Percent_Identity=30.8370044052863, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920448, Length=227, Percent_Identity=30.8370044052863, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921524, Length=235, Percent_Identity=32.7659574468085, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI45549423, Length=136, Percent_Identity=33.8235294117647, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37499; Mature: 37368

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNIRLKGRGVNRKITSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGY
CCCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
GTQKINQIDLEEGPIGVQIFDNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGS
CCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCC
ALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIEDFLFKLQDAGATMITLHGRT
EEHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCH
RKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTKADGVMIGRGILGSPW
HCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCE
KIGEIDYALRENKNFKEPNTEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGA
ECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHCCH
SNLRNNLVRAVDKNEVKNLINKMIQTLNNEKNRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SSNIRLKGRGVNRKITSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGY
CCCEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCC
GTQKINQIDLEEGPIGVQIFDNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGS
CCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCC
ALIKDRKLAIELVKNVVKAVRVPVTVKTRLGWDSKEENIEDFLFKLQDAGATMITLHGRT
EEHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCH
RKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLKKTKADGVMIGRGILGSPW
HCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCE
KIGEIDYALRENKNFKEPNTEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGA
ECCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHCCH
SNLRNNLVRAVDKNEVKNLINKMIQTLNNEKNRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]