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The map label for this gene is pheT [H]

Identifier: 123968524

GI number: 123968524

Start: 862219

End: 864663

Strand: Reverse

Name: pheT [H]

Synonym: A9601_09911

Alternate gene names: 123968524

Gene position: 864663-862219 (Counterclockwise)

Preceding gene: 123968530

Following gene: 123968523

Centisome position: 51.78

GC content: 30.35

Gene sequence:

>2445_bases
ATGAAAGTTTCTCAAAATTGGTTGAATAATTTAGTAGAAATTACTTCTACTCCTGAAGATCTCTCTGAGAAATTGTCTAT
TGGTGGATTTGAGGTTGAATCATTAGAAGATTGTTCAGAAAATGTAAATGGTGTTGTTTTAGGTAAAGTGTTATCTGTTT
TAAAACACGAAGGATCTGACAAGCTTTCAATTTGCCAAGTCGATATTGGTAATTCAAGGAATTTACAAATTATCTGTGGT
GCTCGCAATATTAAACCAAATATTTATGTTTATATTGCTACTGTCGGCGCGAAATTAAATGCTGTTAATTTAAATATTAA
AAGAAGCGAAATTAGAGGTGTCATGAGTGAAGGCATGATATGTTCACTGCAGGAACTAGGTTTAGAGGACTCTAGCGAAG
GTATAGAGATCATTGATGAAGATTTAGCCTCAAAACATGAATTAGGCACTCCAGCGTCTGAATTGCTTCAATTAAATGAT
TTTATATATGATTTAGCCATTACAGCTAATAGACCTGACGGAATGTCAGTTTTGGGTATAGCCCGTGAAATTTCTGCCCT
TTTAGAATCCACTTTAAATTCTCCTGAATTAAACCATAAATACAATATTCAATTACTTAAGGGAATTAAACTTTGTCCAG
AGGCTATAGAATCGAACTGTATTTATACAATAAGCTGCATTGATGGAGTAAATGGAGATAAATTATCGCCAAATTGGCTT
AAAGACCGTATAGAAAAATCAGGTATAAAATCTATTAATCTTCTAGTTGACTTGACAAATTATATTCTTTTAGAACAAGG
TCAACCTTTGCATGCGTTTGATAAAGATAAACTTTCAAACTTAATTGGTAAGGAAGTTTCTCCAGAAGATTTCTCTGTAA
GAAAAGGTAAGGATAATGAAAGACTTATTTGCTTAGATGGTAAAGAATATGACTTAAATGACAATATCACAGTCATTACT
TGTTGTGATAAACCGGTAGCTATTGCAGGGGTGATAGGCGGTATAGAGACTTCTGTAACTAATACTACCTCATCTATTTA
CCTTGAAGGCGCTGTTTTTAATCCAGTTACTATAAGAAAATCTTCAAAGGCGGTTGGCATAAGAACAGAATCTAGCAGCA
GGTATGAAAAAGGGATATCATCAAAAAATACGATTAGTGCAGTAACAAGGGCAATTAATCTTTTAGAACAATATTTTTGT
ATTAATTCACCAATCATCAATACTTCCAATTTAATAAGTAATGAGGATATATTTATTAAACTACGGAGAAATCGAATACA
TAAAATTCTTGGTCCATTAATAATTAACGATCAATCTGAAAAAAGAAATTTATCTGATAATGAAATAGTTGATAAATTAA
AACTTATAGGTTGTACTTTAAAGAATAAAGAATATGGCTGGGATGTAGCAGTAATCCCTAATAGATCGCATGATTTAATA
AGAGAAATAGATTTAATTGAAGAAATAGCGAGATTAATAGGGTATGACAGATTCGACTTAAAACTTCCCAATCCAATTAA
GCCTGGAAAATTGTCATCAGAACAGTTGGCATTGAGAAAAGTAAAAAATGGTTTTATAGATAACGGTTTTAACGAGGTAC
TAAGCTACTCTCTTGTTCCTGAAGATAATGAAAAACTTATAAAGATTTCAAATCCTTTGTTATTAGAAACTAGCTGTCTT
AGAAATAATATCTGGAAAGAACATTTGGAGATAGTGAACCGTAATATAAAAGCTGGACAAGAAAGTTGTTATATATTTGA
AATTGGAAATGTTTTTCATAAAAAAACTGATTTCATTCAAGAAGAAGTTCTGAATGGTGCGATTTATGGAAATAAAAAAT
TTGGAAAATGGTTAAATTCGGGTAAGGATAATCATCTTAATTATTACCAGGCCAGAGGAAAATTAAAGGAGGCTTTATCA
TCTTTGAACATAAAAATTGATGATAAACCTACTGATTCAATTGATTTTCTTCATCCAGGAAGAACAGCGAAATTAATTAT
TGAAGGGAAAGATGCAGGTTATTTTGGTGAAATTCATCCCAAACTAATATTAGAAAAGAAGTCATTAAAAAAAGTTTATT
TATTTAACATTAATGTTTCTAACCTCTTGGAAGCTAGTACAAGAAAAAATAAATGGATTCCAATATATAAGCAATACCCA
ATTGTTCCGAAAATGGAAAGAGATATAAATTTTGTTTTCAGTAAGAAATTTTTAATTAGCGATATAACATCACAGATAAG
AAAAACTGGTAAAAATCTCTTAGAGGATGTAAATTTACTTGATGTTTTTGAAGATACTAAATTTGGAGAGGATCATATAA
GTTATACATTTAGATTATCTTATAGAGATAAAGATAAAACATTATTGGACTCGGACATTACATCAATACATTCAAATATC
ATTTCTAATGTTGAAAAATGTTTCGACACGAAATTGCGGAATTAA

Upstream 100 bases:

>100_bases
TTTAATTAATACTTTATTGTATAATTTTCTATTGTACATCTTCAACAAACTTTTTTGAAGATATACTCATTTCTTTTATA
CTCATTGAATAAAAATATAA

Downstream 100 bases:

>100_bases
TTGTATTACTAATCTTATTTAAGACTATATTCTAGTCTAAATATAATTCTTATATAAGACAAATAATAATCGTATAAAAC
TAATTAATGTTGTATGATCA

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 814; Mature: 814

Protein sequence:

>814_residues
MKVSQNWLNNLVEITSTPEDLSEKLSIGGFEVESLEDCSENVNGVVLGKVLSVLKHEGSDKLSICQVDIGNSRNLQIICG
ARNIKPNIYVYIATVGAKLNAVNLNIKRSEIRGVMSEGMICSLQELGLEDSSEGIEIIDEDLASKHELGTPASELLQLND
FIYDLAITANRPDGMSVLGIAREISALLESTLNSPELNHKYNIQLLKGIKLCPEAIESNCIYTISCIDGVNGDKLSPNWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNERLICLDGKEYDLNDNITVIT
CCDKPVAIAGVIGGIETSVTNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTISAVTRAINLLEQYFC
INSPIINTSNLISNEDIFIKLRRNRIHKILGPLIINDQSEKRNLSDNEIVDKLKLIGCTLKNKEYGWDVAVIPNRSHDLI
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIDNGFNEVLSYSLVPEDNEKLIKISNPLLLETSCL
RNNIWKEHLEIVNRNIKAGQESCYIFEIGNVFHKKTDFIQEEVLNGAIYGNKKFGKWLNSGKDNHLNYYQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLIIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLEASTRKNKWIPIYKQYP
IVPKMERDINFVFSKKFLISDITSQIRKTGKNLLEDVNLLDVFEDTKFGEDHISYTFRLSYRDKDKTLLDSDITSIHSNI
ISNVEKCFDTKLRN

Sequences:

>Translated_814_residues
MKVSQNWLNNLVEITSTPEDLSEKLSIGGFEVESLEDCSENVNGVVLGKVLSVLKHEGSDKLSICQVDIGNSRNLQIICG
ARNIKPNIYVYIATVGAKLNAVNLNIKRSEIRGVMSEGMICSLQELGLEDSSEGIEIIDEDLASKHELGTPASELLQLND
FIYDLAITANRPDGMSVLGIAREISALLESTLNSPELNHKYNIQLLKGIKLCPEAIESNCIYTISCIDGVNGDKLSPNWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNERLICLDGKEYDLNDNITVIT
CCDKPVAIAGVIGGIETSVTNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTISAVTRAINLLEQYFC
INSPIINTSNLISNEDIFIKLRRNRIHKILGPLIINDQSEKRNLSDNEIVDKLKLIGCTLKNKEYGWDVAVIPNRSHDLI
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIDNGFNEVLSYSLVPEDNEKLIKISNPLLLETSCL
RNNIWKEHLEIVNRNIKAGQESCYIFEIGNVFHKKTDFIQEEVLNGAIYGNKKFGKWLNSGKDNHLNYYQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLIIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLEASTRKNKWIPIYKQYP
IVPKMERDINFVFSKKFLISDITSQIRKTGKNLLEDVNLLDVFEDTKFGEDHISYTFRLSYRDKDKTLLDSDITSIHSNI
ISNVEKCFDTKLRN
>Mature_814_residues
MKVSQNWLNNLVEITSTPEDLSEKLSIGGFEVESLEDCSENVNGVVLGKVLSVLKHEGSDKLSICQVDIGNSRNLQIICG
ARNIKPNIYVYIATVGAKLNAVNLNIKRSEIRGVMSEGMICSLQELGLEDSSEGIEIIDEDLASKHELGTPASELLQLND
FIYDLAITANRPDGMSVLGIAREISALLESTLNSPELNHKYNIQLLKGIKLCPEAIESNCIYTISCIDGVNGDKLSPNWL
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNERLICLDGKEYDLNDNITVIT
CCDKPVAIAGVIGGIETSVTNTTSSIYLEGAVFNPVTIRKSSKAVGIRTESSSRYEKGISSKNTISAVTRAINLLEQYFC
INSPIINTSNLISNEDIFIKLRRNRIHKILGPLIINDQSEKRNLSDNEIVDKLKLIGCTLKNKEYGWDVAVIPNRSHDLI
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIDNGFNEVLSYSLVPEDNEKLIKISNPLLLETSCL
RNNIWKEHLEIVNRNIKAGQESCYIFEIGNVFHKKTDFIQEEVLNGAIYGNKKFGKWLNSGKDNHLNYYQARGKLKEALS
SLNIKIDDKPTDSIDFLHPGRTAKLIIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLEASTRKNKWIPIYKQYP
IVPKMERDINFVFSKKFLISDITSQIRKTGKNLLEDVNLLDVFEDTKFGEDHISYTFRLSYRDKDKTLLDSDITSIHSNI
ISNVEKCFDTKLRN

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=838, Percent_Identity=30.1909307875895, Blast_Score=362, Evalue=1e-101,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 91532; Mature: 91532

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVSQNWLNNLVEITSTPEDLSEKLSIGGFEVESLEDCSENVNGVVLGKVLSVLKHEGSD
CCCCHHHHHHHHECCCCHHHHHHHHCCCCEEHHHHHHHHCCCCEEEHHHHHHHHHCCCCC
KLSICQVDIGNSRNLQIICGARNIKPNIYVYIATVGAKLNAVNLNIKRSEIRGVMSEGMI
CEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCEEEEEEEEEEHHHHHHHHHCCCE
CSLQELGLEDSSEGIEIIDEDLASKHELGTPASELLQLNDFIYDLAITANRPDGMSVLGI
EEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECCCCCCCHHHHH
AREISALLESTLNSPELNHKYNIQLLKGIKLCPEAIESNCIYTISCIDGVNGDKLSPNWL
HHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHCHHHHCCCCEEEEEEECCCCCCCCCHHHH
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNE
HHHHHHCCCCEEEHEEEEHHHEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCC
RLICLDGKEYDLNDNITVITCCDKPVAIAGVIGGIETSVTNTTSSIYLEGAVFNPVTIRK
EEEEECCCEECCCCCEEEEEECCCCEEEEHHHCCCHHCCCCCCCEEEEECEEECCEEEEC
SSKAVGIRTESSSRYEKGISSKNTISAVTRAINLLEQYFCINSPIINTSNLISNEDIFIK
CCCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEE
LRRNRIHKILGPLIINDQSEKRNLSDNEIVDKLKLIGCTLKNKEYGWDVAVIPNRSHDLI
EHHHHHHHHHCCEEECCCHHHCCCCHHHHHHHHHHHEEEECCCCCCCEEEEECCCCHHHH
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIDNGFNEVLSYSLVP
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHEEECCC
EDNEKLIKISNPLLLETSCLRNNIWKEHLEIVNRNIKAGQESCYIFEIGNVFHKKTDFIQ
CCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHH
EEVLNGAIYGNKKFGKWLNSGKDNHLNYYQARGKLKEALSSLNIKIDDKPTDSIDFLHPG
HHHHCCEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCC
RTAKLIIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLEASTRKNKWIPIYKQYP
CEEEEEEECCCCCCCCCCCHHEEEECCCCCEEEEEECCHHHHHHHHHCCCCCEEEHCCCC
IVPKMERDINFVFSKKFLISDITSQIRKTGKNLLEDVNLLDVFEDTKFGEDHISYTFRLS
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEEE
YRDKDKTLLDSDITSIHSNIISNVEKCFDTKLRN
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKVSQNWLNNLVEITSTPEDLSEKLSIGGFEVESLEDCSENVNGVVLGKVLSVLKHEGSD
CCCCHHHHHHHHECCCCHHHHHHHHCCCCEEHHHHHHHHCCCCEEEHHHHHHHHHCCCCC
KLSICQVDIGNSRNLQIICGARNIKPNIYVYIATVGAKLNAVNLNIKRSEIRGVMSEGMI
CEEEEEEECCCCCCEEEEEECCCCCCCEEEEEEEECCEEEEEEEEEEHHHHHHHHHCCCE
CSLQELGLEDSSEGIEIIDEDLASKHELGTPASELLQLNDFIYDLAITANRPDGMSVLGI
EEHHHCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECCCCCCCHHHHH
AREISALLESTLNSPELNHKYNIQLLKGIKLCPEAIESNCIYTISCIDGVNGDKLSPNWL
HHHHHHHHHHHCCCCCCCCEEEEEEEHHHHHCHHHHCCCCEEEEEEECCCCCCCCCHHHH
KDRIEKSGIKSINLLVDLTNYILLEQGQPLHAFDKDKLSNLIGKEVSPEDFSVRKGKDNE
HHHHHHCCCCEEEHEEEEHHHEEEECCCCCCCCCHHHHHHHHCCCCCCCCCEEECCCCCC
RLICLDGKEYDLNDNITVITCCDKPVAIAGVIGGIETSVTNTTSSIYLEGAVFNPVTIRK
EEEEECCCEECCCCCEEEEEECCCCEEEEHHHCCCHHCCCCCCCEEEEECEEECCEEEEC
SSKAVGIRTESSSRYEKGISSKNTISAVTRAINLLEQYFCINSPIINTSNLISNEDIFIK
CCCEEEEEECCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEE
LRRNRIHKILGPLIINDQSEKRNLSDNEIVDKLKLIGCTLKNKEYGWDVAVIPNRSHDLI
EHHHHHHHHHCCEEECCCHHHCCCCHHHHHHHHHHHEEEECCCCCCCEEEEECCCCHHHH
REIDLIEEIARLIGYDRFDLKLPNPIKPGKLSSEQLALRKVKNGFIDNGFNEVLSYSLVP
HHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHEEECCC
EDNEKLIKISNPLLLETSCLRNNIWKEHLEIVNRNIKAGQESCYIFEIGNVFHKKTDFIQ
CCCCCEEEECCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCHHHHHHHHHH
EEVLNGAIYGNKKFGKWLNSGKDNHLNYYQARGKLKEALSSLNIKIDDKPTDSIDFLHPG
HHHHCCEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCC
RTAKLIIEGKDAGYFGEIHPKLILEKKSLKKVYLFNINVSNLLEASTRKNKWIPIYKQYP
CEEEEEEECCCCCCCCCCCHHEEEECCCCCEEEEEECCHHHHHHHHHCCCCCEEEHCCCC
IVPKMERDINFVFSKKFLISDITSQIRKTGKNLLEDVNLLDVFEDTKFGEDHISYTFRLS
CCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCEEEEEEE
YRDKDKTLLDSDITSIHSNIISNVEKCFDTKLRN
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA