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The map label for this gene is proB [H]

Identifier: 123968385

GI number: 123968385

Start: 738588

End: 739670

Strand: Reverse

Name: proB [H]

Synonym: A9601_08521

Alternate gene names: 123968385

Gene position: 739670-738588 (Counterclockwise)

Preceding gene: 123968386

Following gene: 123968384

Centisome position: 44.29

GC content: 29.73

Gene sequence:

>1083_bases
ATGAAAACTTGGGTTATAAAAATTGGTACTAGTATTTTAAGAGGAACAGAGGAAACATCTACAGAAGAAGTTATTGAAAA
CCTTTCTAGATCCTTTACAAGTTTTCTTTCAAAAGGAAACAAGTTAATTTTAGTAACTAGTGGAGCCGTTGGATTAGGTT
GCCAAAAGTTAAATATTAAAACAAGACCAAATGATTTAAGTACCCTTCAAGCTACTGCTGCAGTAGGTCAAGTTAATTTA
ATGTCCTTATACGATAAAGTATTTAATAGATTAGGTCTTAATATTGCTCAAATTTTAATAACTAAAGCTGATTTCAATTC
ACGAGAATCCTTTAATAACGCTTCTAAAACTTTAAAAAGATTAATCGATTTGAATGTTATTCCAATAGTAAATGAAAATG
ATACCGTAGCAAATGAAGAGCTTAAATATGGAGATAATGATACCCTCTCTGCTTTAGTTGCCTTGGCTATAAATGCTAAC
AAGCTTATTTTATTAACCGATATTGAAAATCTATACTCAAAAGATCCACGTAATAATAAAGATGCGCAACCTATCAAAGA
AGTTCATAATAGTGAATTAAAGGAAATTAAAGATAAAAATATTCAAAACTCAAATAATGAATGGGGAACAGGAGGAATTT
CTACAAAATTAATTTCTGCAGAAATAGCAACAAAAGGAGGAGTCGAAGTCCAACTAGTTGATGGAACTAATAAAAAAAAC
TTAATTGAAATATTTAATGATAATAAAATTGGAACTTTATTTTATCCAGTAGAAAAACCTATTGGAAACAAAAAAAGTTG
GCTTTCACACGCAATTCAAACAGTAGGGAAAATTACTTTAGATGATGGCGCTTCTTTTGCAATTAAAAAAAAAGGTGCCT
CACTTTTAGCGGTTGGTGTTAAGAATGTAGAAGGAAACTTTACGATTAATCAGGCAGTTAAAATCGTAAATACAAATGAT
AAAGAAGTTGCAAAAGGTTTAGTATCAATAAGTAGCGACAAATTAAGAAGTATCTTAAATAATAAAGAAAATAACAACTC
CTCGATAATTGTCGTTCATAGAGATGTTCTTGCTCTCTCTTAG

Upstream 100 bases:

>100_bases
ATATAAAAACAATATTAGTTAAGAGATTAAATAGAGATGGCCTGCCTATAAAATTTAATTTAACTTTGACAATAGAAAAA
TTAATTTCTCATTTTATAAA

Downstream 100 bases:

>100_bases
AAAAAATTAAAACTGAAAAATGCTTTTAAGTGATTTAGTTGATCTAATAAAAAAAGGAAATTCAAATTTTATTAGTGCCA
ATATTTTCGAAGATTTAAAT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKVFNRLGLNIAQILITKADFNSRESFNNASKTLKRLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGVKNVEGNFTINQAVKIVNTND
KEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS

Sequences:

>Translated_360_residues
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKVFNRLGLNIAQILITKADFNSRESFNNASKTLKRLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGVKNVEGNFTINQAVKIVNTND
KEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS
>Mature_360_residues
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIKTRPNDLSTLQATAAVGQVNL
MSLYDKVFNRLGLNIAQILITKADFNSRESFNNASKTLKRLIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINAN
KLILLTDIENLYSKDPRNNKDAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGVKNVEGNFTINQAVKIVNTND
KEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=29.9319727891156, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=29.7297297297297, Blast_Score=118, Evalue=7e-27,
Organism=Escherichia coli, GI1786437, Length=363, Percent_Identity=37.1900826446281, Blast_Score=259, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI17569969, Length=312, Percent_Identity=28.2051282051282, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17569967, Length=312, Percent_Identity=28.2051282051282, Blast_Score=118, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320506, Length=354, Percent_Identity=32.2033898305085, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6321822, Length=232, Percent_Identity=42.2413793103448, Blast_Score=169, Evalue=7e-43,
Organism=Drosophila melanogaster, GI21357643, Length=291, Percent_Identity=32.9896907216495, Blast_Score=134, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39270; Mature: 39270

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIK
CCEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEE
TRPNDLSTLQATAAVGQVNLMSLYDKVFNRLGLNIAQILITKADFNSRESFNNASKTLKR
ECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEHHEEEECCCCCCHHHHHHHHHHHH
LIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINANKLILLTDIENLYSKDPRNNK
HHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEECCCEEEEEECCHHHHCCCCCCCC
DAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHCCCCCCEEEEECCCCCCC
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGV
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCEEEECCCCCEEEECCCCEEEEEEE
KNVEGNFTINQAVKIVNTNDKEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS
ECCCCCEEEEHEEEEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEECCEEECC
>Mature Secondary Structure
MKTWVIKIGTSILRGTEETSTEEVIENLSRSFTSFLSKGNKLILVTSGAVGLGCQKLNIK
CCEEEEEECHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEEE
TRPNDLSTLQATAAVGQVNLMSLYDKVFNRLGLNIAQILITKADFNSRESFNNASKTLKR
ECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEHHEEEECCCCCCHHHHHHHHHHHH
LIDLNVIPIVNENDTVANEELKYGDNDTLSALVALAINANKLILLTDIENLYSKDPRNNK
HHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHEECCCEEEEEECCHHHHCCCCCCCC
DAQPIKEVHNSELKEIKDKNIQNSNNEWGTGGISTKLISAEIATKGGVEVQLVDGTNKKN
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEHCCCCCCEEEEECCCCCCC
LIEIFNDNKIGTLFYPVEKPIGNKKSWLSHAIQTVGKITLDDGASFAIKKKGASLLAVGV
EEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCEEEECCCCCEEEECCCCEEEEEEE
KNVEGNFTINQAVKIVNTNDKEVAKGLVSISSDKLRSILNNKENNNSSIIVVHRDVLALS
ECCCCCEEEEHEEEEEECCHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEECCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA