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The map label for this gene is ilvD [H]

Identifier: 123968369

GI number: 123968369

Start: 724972

End: 726645

Strand: Reverse

Name: ilvD [H]

Synonym: A9601_08361

Alternate gene names: 123968369

Gene position: 726645-724972 (Counterclockwise)

Preceding gene: 123968370

Following gene: 123968361

Centisome position: 43.51

GC content: 36.74

Gene sequence:

>1674_bases
ATGAATAAACTCAGATCATCTGCAATAACCCAAGGTGTGCAAAGATCACCTAACAGATCGATGTTAAGAGCTGTTGGATT
TAATGATGAAGATTTTAATAAACCTATTATTGGAGTTGCAAATGGATACAGCACCATAACACCATGCAATATGGGTTTAA
ATAAGTTAGCTCTAAAAGCTGAAGAGTCTATAAAAAGATCAGGTGGGATGCCTCAGATGTTTGGGACTATAACAGTAAGT
GATGGGATTTCTATGGGAACAGAGGGCATGAAATATTCCCTAGTTTCAAGAGAAGTTATTGCTGATTCAATTGAAACAGC
ATGCAATGCTCAGAGTATGGATGGAGTACTTGCTATAGGTGGATGTGATAAAAATATGCCGGGTGCCATGATTGCGATTG
CAAGAATGAATATTCCATCAATTTTCATTTATGGAGGAACAATAAAGCCTGGGAAATTGCATGGAGAAGATCTTACTGTT
GTTAGTGCATTTGAAGCTGTTGGACAATTAACATCAGGCAAAATTAATGAAGAAAGGCTAATCCAAGTTGAGAAAAATTG
TATTCCTGGTGCTGGTAGCTGTGGAGGAATGTTTACAGCTAATACAATGTCTGCGGTTATTGAAGTATTAGGGTTAAGTC
TTCCTCACAGTTCCACTATGGCTGCTGAAGATCTTGAAAAAGAACTAAGTGCAGACAAAAGTGCTGAGATATTAGTCTCC
GCAATAGAAAAAGATATAAGACCTCTAGACCTAATGACTAAGAAAGCATTTGAAAATGCAATATCAGTAATTATGGCAAT
TGGCGGATCAACAAATGCGGTATTGCACATCTTAGCTATCGCGAATACTGCAGGAATAGATATCAACATTAATGATTTTG
AGAGAATCAGACAAAAAGTACCCGTTATTTGTGACCTTAAACCGAGTGGTAAATATGTGACGGTGGATCTTCATAAGGCA
GGTGGGATTCCACAAGTAATGAAAATACTTTTGAATGCAGGATTAATTCATGGCGATTGCAAAAACATTGAAGGAAAAAC
CATCTCAGAATACTTACAAAATATTCCAGATAAGCCTCCAACAAATCAAAATGTCATAAGAGACATAGATAACCCTCTTT
ATAAAAAAGGACATCTAGCGATATTAAAAGGTAACTTAGCGAGCGAAGGTTCTGTAGCCAAAATTAGCGGAGTAAAAAAC
CCTGTATTAACAGGTCCCGCAAAAATTTTTGAAAGTGAAGAAGATTGTTTAAAATCGATATTAAATAACGATATCAAAGC
TGGTGATGTTGTTGTTATTAGAAACGAAGGTCCTGTAGGAGGACCAGGTATGAGAGAGATGTTAGCTCCAACATCTGCAA
TTGTTGGTCAAGGGCTAGGAGAGAAGGTGGCTTTAATTACCGATGGCAGATTTAGCGGCGGTACCTATGGTCTTGTTGTG
GGTCACATAGCTCCAGAGGCTGCTGTAGGAGGAAATATTGCTCTAATAAAACAAGGTGATTTAATTACAGTAGATGCTGT
AAAACAACTAATTGAAGTTGATTTATCTGACGAAGAATTAGAAAAAAGAAAAAAAGATTGGGTAAAACCTATTCAAAAAT
ACAAAAGAGGAATTCTTTCAAAATATTCGAGAATCGTAAGCACATCAAGTTTAGGGGCTGTTACTGATTTATAA

Upstream 100 bases:

>100_bases
ATCTGGAGAAGAAAGTTGAGCAACTTAATTCTGCAATTGCTGATGTGAGGAAGCAACTTGAAGAGTTGGGGCAATAGAAT
AAAAAAGGTTCTCAAATAAT

Downstream 100 bases:

>100_bases
ATCAGCTCAAATTTTTTTTCTTAATCAATAAAACTTTTTATCCTATTTGCTAAATTTTCCAATCTAGCGCTGTATTTCGG
TGAATTGCATTTTTTCCCAT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIIGVANGYSTITPCNMGLNKLALKAEESIKRSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTV
VSAFEAVGQLTSGKINEERLIQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKVPVICDLKPSGKYVTVDLHKA
GGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPPTNQNVIRDIDNPLYKKGHLAILKGNLASEGSVAKISGVKN
PVLTGPAKIFESEEDCLKSILNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILSKYSRIVSTSSLGAVTDL

Sequences:

>Translated_557_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIIGVANGYSTITPCNMGLNKLALKAEESIKRSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTV
VSAFEAVGQLTSGKINEERLIQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKVPVICDLKPSGKYVTVDLHKA
GGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPPTNQNVIRDIDNPLYKKGHLAILKGNLASEGSVAKISGVKN
PVLTGPAKIFESEEDCLKSILNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILSKYSRIVSTSSLGAVTDL
>Mature_557_residues
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIIGVANGYSTITPCNMGLNKLALKAEESIKRSGGMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIGGCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTV
VSAFEAVGQLTSGKINEERLIQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKVPVICDLKPSGKYVTVDLHKA
GGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPPTNQNVIRDIDNPLYKKGHLAILKGNLASEGSVAKISGVKN
PVLTGPAKIFESEEDCLKSILNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILSKYSRIVSTSSLGAVTDL

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=610, Percent_Identity=39.672131147541, Blast_Score=411, Evalue=1e-116,
Organism=Escherichia coli, GI1788157, Length=478, Percent_Identity=30.9623430962343, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI2367371, Length=510, Percent_Identity=33.921568627451, Blast_Score=211, Evalue=1e-55,
Organism=Escherichia coli, GI1786464, Length=502, Percent_Identity=32.6693227091633, Blast_Score=202, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6322476, Length=564, Percent_Identity=46.6312056737589, Blast_Score=507, Evalue=1e-144,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59091; Mature: 59091

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIIGVANGYSTITPCNMGLNKLALKA
CCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHH
EESIKRSGGMPQMFGTITVSDGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIG
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTVVSAFEAVGQLTSGKINEERL
CCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
IQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
HEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKV
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCEEEECHHHHHHHHHC
PVICDLKPSGKYVTVDLHKAGGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPP
CEEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHCCCCCC
TNQNVIRDIDNPLYKKGHLAILKGNLASEGSVAKISGVKNPVLTGPAKIFESEEDCLKSI
CCCHHHHHCCCHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHH
LNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
HCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEE
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILS
ECCCCCHHCCCCEEEEECCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KYSRIVSTSSLGAVTDL
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNKLRSSAITQGVQRSPNRSMLRAVGFNDEDFNKPIIGVANGYSTITPCNMGLNKLALKA
CCHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHH
EESIKRSGGMPQMFGTITVSDGISMGTEGMKYSLVSREVIADSIETACNAQSMDGVLAIG
HHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCCCCCCEEEEC
GCDKNMPGAMIAIARMNIPSIFIYGGTIKPGKLHGEDLTVVSAFEAVGQLTSGKINEERL
CCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHH
IQVEKNCIPGAGSCGGMFTANTMSAVIEVLGLSLPHSSTMAAEDLEKELSADKSAEILVS
HEECCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHH
AIEKDIRPLDLMTKKAFENAISVIMAIGGSTNAVLHILAIANTAGIDININDFERIRQKV
HHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEEEECCCCEEEECHHHHHHHHHC
PVICDLKPSGKYVTVDLHKAGGIPQVMKILLNAGLIHGDCKNIEGKTISEYLQNIPDKPP
CEEEEECCCCCEEEEEECCCCCCHHHHHHHHHCCEEECCCCCCCCCHHHHHHHHCCCCCC
TNQNVIRDIDNPLYKKGHLAILKGNLASEGSVAKISGVKNPVLTGPAKIFESEEDCLKSI
CCCHHHHHCCCHHHCCCCEEEEECCCCCCCCEEEECCCCCCCCCCHHHHHCCHHHHHHHH
LNNDIKAGDVVVIRNEGPVGGPGMREMLAPTSAIVGQGLGEKVALITDGRFSGGTYGLVV
HCCCCCCCCEEEEECCCCCCCCCHHHHHCCHHHHHHCCCCCEEEEEECCCCCCCEEEEEE
GHIAPEAAVGGNIALIKQGDLITVDAVKQLIEVDLSDEELEKRKKDWVKPIQKYKRGILS
ECCCCCHHCCCCEEEEECCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
KYSRIVSTSSLGAVTDL
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA