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The map label for this gene is yafV [C]

Identifier: 123968206

GI number: 123968206

Start: 595842

End: 596669

Strand: Direct

Name: yafV [C]

Synonym: A9601_06711

Alternate gene names: 123968206

Gene position: 595842-596669 (Clockwise)

Preceding gene: 123968205

Following gene: 123968207

Centisome position: 35.68

GC content: 37.2

Gene sequence:

>828_bases
TTGACTGATTTTTTGGTAGCTGCATTACAAATTACGAGTACTTCAAATGTTGAAGCAAATTTTACTGAAGCAGAAGAACA
GATTGAATTAGCTGCTAGAAGAGGTGCTGAGTTAATAGGATTGCCTGAGAATTTTGCTTTTTTAGGAGGAGATGATGAAA
AACTTAGATTAGCTTCTGAATTATCAGTGAAGTGTACAAATTTTCTAAAAACCATGTCACAAAGATATCAAGTATTTCTA
TTGGGAGGAGGGTATCCTGTCCCTGCTGGTGATGATAGTCATACTTTTAATAGGTCAGCTTTATTTGGGAAAGATGGACA
GATTTTGGCAAAATACGACAAGATTCATTTGTTTGATGTTGATTTGCCAGATGGAAATCTATACAAGGAATCATCCACTA
TTTTATCTGGAGCAGAGTATCCACCTGTTGTAGATGTCCCAGGTTTATGCAAAATAGGATTATCGATTTGTTACGACGTT
AGATTTCCTGAACTCTATAGATATTTGTCTTCCAATGGTGCAGAGCTAATTATGATTCCCGCAGCTTTTACAGCTTTTAC
TGGAAAAGATCATTGGCAAATCCTATTACAAGCAAGAGCAATTGAGAATACAGCATATGTAGTCGCTCCAGCTCAAACTG
GTATTCATTATGGAAGAAGGCAAAGTCATGGCCATGCGATGGTAATTGACCCTTGGGGTACAGTTTTATCTGATGCCGGA
AAAACTCAGGGGGCTGCAATAGCACCTGCTGATAAAGAAAGAGTAAAGAAAATTAGGGAGCAGATGCCAAGCCTTAAACA
TAGAAAAAACAAATTGTTTTCAAACTAA

Upstream 100 bases:

>100_bases
TTGCTCTGAACTTGATTGCTTAGATATTGTTCCAGCTCAAGTTGAAAGAGGTGTGATTCGTGCCTCATGATCTACGAATT
GGTTCATTAGGAGTAAAGTC

Downstream 100 bases:

>100_bases
TGATAAAGTTTTTAGAAAATAAACTTCTTCATTATTTATCCGTTTTTTTATTTTTAAATTCTTCAATCTTTCCAGTTAAA
TCTTCAAGTGCACTTGCGGC

Product: putative nitrilase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTDFLVAALQITSTSNVEANFTEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASELSVKCTNFLKTMSQRYQVFL
LGGGYPVPAGDDSHTFNRSALFGKDGQILAKYDKIHLFDVDLPDGNLYKESSTILSGAEYPPVVDVPGLCKIGLSICYDV
RFPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
KTQGAAIAPADKERVKKIREQMPSLKHRKNKLFSN

Sequences:

>Translated_275_residues
MTDFLVAALQITSTSNVEANFTEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASELSVKCTNFLKTMSQRYQVFL
LGGGYPVPAGDDSHTFNRSALFGKDGQILAKYDKIHLFDVDLPDGNLYKESSTILSGAEYPPVVDVPGLCKIGLSICYDV
RFPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
KTQGAAIAPADKERVKKIREQMPSLKHRKNKLFSN
>Mature_274_residues
TDFLVAALQITSTSNVEANFTEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASELSVKCTNFLKTMSQRYQVFLL
GGGYPVPAGDDSHTFNRSALFGKDGQILAKYDKIHLFDVDLPDGNLYKESSTILSGAEYPPVVDVPGLCKIGLSICYDVR
FPELYRYLSSNGAELIMIPAAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAGK
TQGAAIAPADKERVKKIREQMPSLKHRKNKLFSN

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=275, Percent_Identity=38.9090909090909, Blast_Score=197, Evalue=6e-51,
Organism=Homo sapiens, GI5031947, Length=275, Percent_Identity=38.9090909090909, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI297632350, Length=275, Percent_Identity=38.9090909090909, Blast_Score=197, Evalue=8e-51,
Organism=Homo sapiens, GI9910460, Length=274, Percent_Identity=33.9416058394161, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI297632346, Length=193, Percent_Identity=36.7875647668394, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1786412, Length=249, Percent_Identity=28.1124497991968, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17556280, Length=272, Percent_Identity=39.7058823529412, Blast_Score=211, Evalue=4e-55,
Organism=Saccharomyces cerevisiae, GI6322335, Length=302, Percent_Identity=33.7748344370861, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6323383, Length=282, Percent_Identity=32.2695035460993, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17933642, Length=271, Percent_Identity=35.4243542435424, Blast_Score=166, Evalue=1e-41,
Organism=Drosophila melanogaster, GI21355835, Length=272, Percent_Identity=32.3529411764706, Blast_Score=152, Evalue=3e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30270; Mature: 30139

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS50263 CN_HYDROLASE ; PS01227 UPF0012

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFLVAALQITSTSNVEANFTEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASE
CCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCHHEEEHHH
LSVKCTNFLKTMSQRYQVFLLGGGYPVPAGDDSHTFNRSALFGKDGQILAKYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCEECCCCCCCCCCCCCEEECCCCCEEEEECEEEEEEE
DLPDGNLYKESSTILSGAEYPPVVDVPGLCKIGLSICYDVRFPELYRYLSSNGAELIMIP
ECCCCCCEECCCCEECCCCCCCCCCCCCHHHHHHHHEEECCCHHHHHHHHCCCCEEEEEE
AAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
CEEEECCCCCHHEEEEEHEEECCEEEEEEECHHCCCCCCCCCCCCEEEECCCHHHHHCCC
KTQGAAIAPADKERVKKIREQMPSLKHRKNKLFSN
CCCCCEECCCCHHHHHHHHHHCCHHHHHHHHCCCC
>Mature Secondary Structure 
TDFLVAALQITSTSNVEANFTEAEEQIELAARRGAELIGLPENFAFLGGDDEKLRLASE
CCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEECCCCHHEEEHHH
LSVKCTNFLKTMSQRYQVFLLGGGYPVPAGDDSHTFNRSALFGKDGQILAKYDKIHLFDV
HHHHHHHHHHHHHHCEEEEEEECCEECCCCCCCCCCCCCEEECCCCCEEEEECEEEEEEE
DLPDGNLYKESSTILSGAEYPPVVDVPGLCKIGLSICYDVRFPELYRYLSSNGAELIMIP
ECCCCCCEECCCCEECCCCCCCCCCCCCHHHHHHHHEEECCCHHHHHHHHCCCCEEEEEE
AAFTAFTGKDHWQILLQARAIENTAYVVAPAQTGIHYGRRQSHGHAMVIDPWGTVLSDAG
CEEEECCCCCHHEEEEEHEEECCEEEEEEECHHCCCCCCCCCCCCEEEECCCHHHHHCCC
KTQGAAIAPADKERVKKIREQMPSLKHRKNKLFSN
CCCCCEECCCCHHHHHHHHHHCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]