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The map label for this gene is murF [H]

Identifier: 123968201

GI number: 123968201

Start: 589968

End: 591341

Strand: Direct

Name: murF [H]

Synonym: A9601_06661

Alternate gene names: 123968201

Gene position: 589968-591341 (Clockwise)

Preceding gene: 123968200

Following gene: 123968205

Centisome position: 35.33

GC content: 27.73

Gene sequence:

>1374_bases
ATGGAATTCTCTATATCAGAAATAAACAATGTTTTGGGAGATATTAAAAATCTGAGCGAGGGGAAAAAAGATTTTTTAAA
TTTTAAGAATATAAGTATTGATAGTAGAACTTTATTAAAAAATGATCTTTTTATAGCCATCAAGGGTAAAAATTTTGATG
GACATGGTTTTCTTTCAGAGGTTTTAAATAAAGGAGTTAAATCTGTCGTAATTAAAAAAGGGATGCAGAAATTACTTCCT
AGTAATTTTCCTTATTGGGTTGTAAATGACACAACAGAGGCATTTCAAAAATTAGCATTGCTAAAAAGAAAAAAATTAAA
TATTCCTATTGTTGCAATAACTGGCTCAGTGGGTAAAACAACTACAAAAGAAATGATTGGTGGAGTTTTAAATAAACTTG
GAAGAATTAAATTATCTCATGCAAATTTCAATAACGAGATTGGAGTTGGTCTTACTATTCTTGCTGCAGATATAGAAGAT
AAAGTTTTAGTTCTTGAAATGGGGATGAGAGGTCTTGGACAGATCGAGAATTTATCCAAATATAGTGAACCAGATATTGC
AGTTATTACTAACATTGGCACAGCCCATATTGGATTGTTAGGTTCAAAAGAAAATATTACTCATGCAAAGTGTGAAATTA
GTAAGTTCTTAAATCCAGAAGGAGTTGTAATTATTCCAGCTAATGATCCATTCCTAGAAAAAACTTTAAAAGAATTTTGG
AAAGGTAGAGTTATAAAAGTAAGGCTATTAAATATAGCAAATCAAAAGGAGAGTATTAAGAAGGATGATAATTTGCGAGG
ATTTTATAATCCTTCAAATAAAACAATTTTAATTGAAGAAAATACCTTTGAAATTTCATTTGAGGGATTTCACAATGCTT
CTAATTTCTTATTTGCTTATGCAGTTGCTAAAGAGCTAGGCATTGATTTTGCAAGTTCTAATAAATTTGATTTTGTAAGT
TTAGATGGAAGGAATAAAATTCTTAAATCAGTAAAAACAACAATATATGATGAATCATATAATGCTTCGCCAGAGTCAGT
AAAAGCATGTATTAAAAACCTCCTTGAAAAACCGAGAAATAAATTTTTCATATTTGGAAGCATGCAGGAATTGGGTAAAG
AATCTGAAAAATATCACAAAGAAATATTCAACTTAATAAATAATTCAGATATAAAAAAGTGTTTATTTATTTGCGAAAAA
GAAAATGAAAAAAATTACACCAATTTTTTAAAAGATAAGAAAAAATTCTTAGTTTTAAATAATATCAAAGATGTACCTCA
AGAGATAAACAAATCTACAAAAAAAGGCGATTCTATTCTTGTAAAAGGAAGCAGATTTTGGCAACTTGATAAAATTATTG
AATTAATTAACTAG

Upstream 100 bases:

>100_bases
CGGAATAAAAGAACCATCACCAGATGTTGCTGAAGTTGAAAAATTTTCTTACGGACAATCAGCTGATCCATCTTTAAAAA
AAGTATGACTTAATTTTTTA

Downstream 100 bases:

>100_bases
ATCAGGATTTCTTCTTTTTCCAATTCTCTATATTTACTTGCTTAGTTCTTGATATCGCTAATGAATTATCTTTAGAGTCC
TTTGTGATCACTGAACCAGC

Product: UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MEFSISEINNVLGDIKNLSEGKKDFLNFKNISIDSRTLLKNDLFIAIKGKNFDGHGFLSEVLNKGVKSVVIKKGMQKLLP
SNFPYWVVNDTTEAFQKLALLKRKKLNIPIVAITGSVGKTTTKEMIGGVLNKLGRIKLSHANFNNEIGVGLTILAADIED
KVLVLEMGMRGLGQIENLSKYSEPDIAVITNIGTAHIGLLGSKENITHAKCEISKFLNPEGVVIIPANDPFLEKTLKEFW
KGRVIKVRLLNIANQKESIKKDDNLRGFYNPSNKTILIEENTFEISFEGFHNASNFLFAYAVAKELGIDFASSNKFDFVS
LDGRNKILKSVKTTIYDESYNASPESVKACIKNLLEKPRNKFFIFGSMQELGKESEKYHKEIFNLINNSDIKKCLFICEK
ENEKNYTNFLKDKKKFLVLNNIKDVPQEINKSTKKGDSILVKGSRFWQLDKIIELIN

Sequences:

>Translated_457_residues
MEFSISEINNVLGDIKNLSEGKKDFLNFKNISIDSRTLLKNDLFIAIKGKNFDGHGFLSEVLNKGVKSVVIKKGMQKLLP
SNFPYWVVNDTTEAFQKLALLKRKKLNIPIVAITGSVGKTTTKEMIGGVLNKLGRIKLSHANFNNEIGVGLTILAADIED
KVLVLEMGMRGLGQIENLSKYSEPDIAVITNIGTAHIGLLGSKENITHAKCEISKFLNPEGVVIIPANDPFLEKTLKEFW
KGRVIKVRLLNIANQKESIKKDDNLRGFYNPSNKTILIEENTFEISFEGFHNASNFLFAYAVAKELGIDFASSNKFDFVS
LDGRNKILKSVKTTIYDESYNASPESVKACIKNLLEKPRNKFFIFGSMQELGKESEKYHKEIFNLINNSDIKKCLFICEK
ENEKNYTNFLKDKKKFLVLNNIKDVPQEINKSTKKGDSILVKGSRFWQLDKIIELIN
>Mature_457_residues
MEFSISEINNVLGDIKNLSEGKKDFLNFKNISIDSRTLLKNDLFIAIKGKNFDGHGFLSEVLNKGVKSVVIKKGMQKLLP
SNFPYWVVNDTTEAFQKLALLKRKKLNIPIVAITGSVGKTTTKEMIGGVLNKLGRIKLSHANFNNEIGVGLTILAADIED
KVLVLEMGMRGLGQIENLSKYSEPDIAVITNIGTAHIGLLGSKENITHAKCEISKFLNPEGVVIIPANDPFLEKTLKEFW
KGRVIKVRLLNIANQKESIKKDDNLRGFYNPSNKTILIEENTFEISFEGFHNASNFLFAYAVAKELGIDFASSNKFDFVS
LDGRNKILKSVKTTIYDESYNASPESVKACIKNLLEKPRNKFFIFGSMQELGKESEKYHKEIFNLINNSDIKKCLFICEK
ENEKNYTNFLKDKKKFLVLNNIKDVPQEINKSTKKGDSILVKGSRFWQLDKIIELIN

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=443, Percent_Identity=25.9593679458239, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 51559; Mature: 51559

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFSISEINNVLGDIKNLSEGKKDFLNFKNISIDSRTLLKNDLFIAIKGKNFDGHGFLSE
CCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHEECCCEEEEEECCCCCCCHHHHH
VLNKGVKSVVIKKGMQKLLPSNFPYWVVNDTTEAFQKLALLKRKKLNIPIVAITGSVGKT
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
TTKEMIGGVLNKLGRIKLSHANFNNEIGVGLTILAADIEDKVLVLEMGMRGLGQIENLSK
HHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHH
YSEPDIAVITNIGTAHIGLLGSKENITHAKCEISKFLNPEGVVIIPANDPFLEKTLKEFW
CCCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
KGRVIKVRLLNIANQKESIKKDDNLRGFYNPSNKTILIEENTFEISFEGFHNASNFLFAY
CCCEEEEEEEEHHCHHHHHCCCCCCCCEECCCCCEEEEECCEEEEEECCCCCCCHHHHHH
AVAKELGIDFASSNKFDFVSLDGRNKILKSVKTTIYDESYNASPESVKACIKNLLEKPRN
HHHHHHCCEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
KFFIFGSMQELGKESEKYHKEIFNLINNSDIKKCLFICEKENEKNYTNFLKDKKKFLVLN
CEEEEECHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCHHEEEEC
NIKDVPQEINKSTKKGDSILVKGSRFWQLDKIIELIN
CHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHC
>Mature Secondary Structure
MEFSISEINNVLGDIKNLSEGKKDFLNFKNISIDSRTLLKNDLFIAIKGKNFDGHGFLSE
CCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHEECCCEEEEEECCCCCCCHHHHH
VLNKGVKSVVIKKGMQKLLPSNFPYWVVNDTTEAFQKLALLKRKKLNIPIVAITGSVGKT
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
TTKEMIGGVLNKLGRIKLSHANFNNEIGVGLTILAADIEDKVLVLEMGMRGLGQIENLSK
HHHHHHHHHHHHHCCEEEEECCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHH
YSEPDIAVITNIGTAHIGLLGSKENITHAKCEISKFLNPEGVVIIPANDPFLEKTLKEFW
CCCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHH
KGRVIKVRLLNIANQKESIKKDDNLRGFYNPSNKTILIEENTFEISFEGFHNASNFLFAY
CCCEEEEEEEEHHCHHHHHCCCCCCCCEECCCCCEEEEECCEEEEEECCCCCCCHHHHHH
AVAKELGIDFASSNKFDFVSLDGRNKILKSVKTTIYDESYNASPESVKACIKNLLEKPRN
HHHHHHCCEECCCCCEEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCC
KFFIFGSMQELGKESEKYHKEIFNLINNSDIKKCLFICEKENEKNYTNFLKDKKKFLVLN
CEEEEECHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHCCHHEEEEC
NIKDVPQEINKSTKKGDSILVKGSRFWQLDKIIELIN
CHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7894708; 8905231 [H]