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The map label for this gene is ispD [H]

Identifier: 123968047

GI number: 123968047

Start: 444615

End: 445286

Strand: Direct

Name: ispD [H]

Synonym: A9601_05101

Alternate gene names: 123968047

Gene position: 444615-445286 (Clockwise)

Preceding gene: 123968045

Following gene: 123968050

Centisome position: 26.63

GC content: 33.04

Gene sequence:

>672_bases
GTGCACTTTTTAATACCAGCTGCAGGGAGTGGTAGCAGAATGAAAGCTGGAAAAAATAAATTACTTATTGATTTAGAGGG
AGAGTCTTTGATTTATTGGACGCTTAAATCCGTACTTTCTGCAAACTCAACAAACTGGGTTGGAATAATTGGGCAACCGA
AAGATAAAAATTTATTATTAAATTCAGCAAAGGATTTTGCCCAAAAAGTTCATTGGATTAATGGTGGTGACACCAGACAA
CAGTCAGTTTTTAATGGTTTAGAAGCGTTACCAAAAGATGCTAAAAAAGTTTTAATACATGATGGTGCTAGATGTCTAAT
TAATCCTGAATTGATAGACCTTTGTGCCAAGCAATTAGATGAAAATGAAGCTGTAATTTTGGCTACTAAGGTAACTGACA
CAATAAAGATTGTTGATAAAGAAGGTTTTATTAAAGAAACACCAGATAGAAATTATTTATGGGCAGCGCAAACTCCTCAG
GGCTTTTTAGTAGATAGATTAAAAAAAGCTCATAAGATGGCAATTGATAAAAAATGGAAAGTCACAGATGATGCCTCACT
ATTCGAAATACTTAATTGGAAAGTGAAGATTATTGAAGGAACTTATTCAAATATAAAAATTACATCCCCTATAGATTTGA
AAATAGCAAAACTTTTTGTGAAGAACCCCTAG

Upstream 100 bases:

>100_bases
ATTGGACATTATGAAATTTAATAATTTTCAATCGTAGTACTATTTGTGTAAAGATATTGCTTTTATTTATAATTGTCTAG
AAAATATAAATCCTTCTATT

Downstream 100 bases:

>100_bases
TTAAAAAGATGTATAGATACTAAGAATGCCATCATCACCATTTAAAGTGGCTTCGTATCCTAAAGGAATGCAAGCATTTC
CCGATATGTGACCTATTGGG

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVLSANSTNWVGIIGQPKDKNLLLNSAKDFAQKVHWINGGDTRQ
QSVFNGLEALPKDAKKVLIHDGARCLINPELIDLCAKQLDENEAVILATKVTDTIKIVDKEGFIKETPDRNYLWAAQTPQ
GFLVDRLKKAHKMAIDKKWKVTDDASLFEILNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNP

Sequences:

>Translated_223_residues
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVLSANSTNWVGIIGQPKDKNLLLNSAKDFAQKVHWINGGDTRQ
QSVFNGLEALPKDAKKVLIHDGARCLINPELIDLCAKQLDENEAVILATKVTDTIKIVDKEGFIKETPDRNYLWAAQTPQ
GFLVDRLKKAHKMAIDKKWKVTDDASLFEILNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNP
>Mature_223_residues
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVLSANSTNWVGIIGQPKDKNLLLNSAKDFAQKVHWINGGDTRQ
QSVFNGLEALPKDAKKVLIHDGARCLINPELIDLCAKQLDENEAVILATKVTDTIKIVDKEGFIKETPDRNYLWAAQTPQ
GFLVDRLKKAHKMAIDKKWKVTDDASLFEILNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNP

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=219, Percent_Identity=32.8767123287671, Blast_Score=107, Evalue=5e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 24949; Mature: 24949

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVLSANSTNWVGIIGQPKDKNLLL
CEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHHCCCCCCEEEEEECCCCCCEEE
NSAKDFAQKVHWINGGDTRQQSVFNGLEALPKDAKKVLIHDGARCLINPELIDLCAKQLD
HHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCHHHEEEEECCCEEEECHHHHHHHHHHCC
ENEAVILATKVTDTIKIVDKEGFIKETPDRNYLWAAQTPQGFLVDRLKKAHKMAIDKKWK
CCCEEEEEEECCCEEEEEECCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEE
VTDDASLFEILNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNP
ECCCHHHHHHHCCEEEEEECCCCCEEECCCCCEEEEEEEECCC
>Mature Secondary Structure
MHFLIPAAGSGSRMKAGKNKLLIDLEGESLIYWTLKSVLSANSTNWVGIIGQPKDKNLLL
CEEEEECCCCCCCCCCCCCEEEEEECCCEEEEEEHHHHHCCCCCCEEEEEECCCCCCEEE
NSAKDFAQKVHWINGGDTRQQSVFNGLEALPKDAKKVLIHDGARCLINPELIDLCAKQLD
HHHHHHHHHEEECCCCCHHHHHHHHHHHHCCCHHHEEEEECCCEEEECHHHHHHHHHHCC
ENEAVILATKVTDTIKIVDKEGFIKETPDRNYLWAAQTPQGFLVDRLKKAHKMAIDKKWK
CCCEEEEEEECCCEEEEEECCCCEECCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCEE
VTDDASLFEILNWKVKIIEGTYSNIKITSPIDLKIAKLFVKNP
ECCCHHHHHHHCCEEEEEECCCCCEEECCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA