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The map label for this gene is rnhA [H]

Identifier: 123967755

GI number: 123967755

Start: 204081

End: 204800

Strand: Reverse

Name: rnhA [H]

Synonym: A9601_02181

Alternate gene names: 123967755

Gene position: 204800-204081 (Counterclockwise)

Preceding gene: 123967756

Following gene: 123967754

Centisome position: 12.26

GC content: 31.81

Gene sequence:

>720_bases
ATGAATAGTGATAGTATTGCGATTGAAGCCGCAACCGATGGAGCCTGCAGCGGTAATCCAGGCCCAGGTGGTTGGGGCGG
TTTAATAATTTTTGACGATAACAGCGAATTAGAAATAGGTGGGTCCGAGCAAAATACTACTAATAATAGAATGGAACTCA
CGGCGGCTATAAAAACTCTTGAGAAATTAAAAACCTACAAATTAAAAGAGAACTTTAAACTAAGAACTGATAGTAAATAT
GTCATAGAGGGTTATACAAAATGGATTATTAATTGGAAGAAAAATGGATGGAAAACAAGTTCAGGAAAACCAGTTCAAAA
TCTTGATCTATGGCAAAAAATTGATCAATTAAGAATTAATGGCCTAATAATGGAATATGTTAAAGGTCATAGCGGGGATA
AACAAAATGATAGGGTTGATAAAATTGCAACTAATTACAGCAAAGGTATATCTATAGAAAGTAACTTAAAAAAAGTAGAA
TACTCAGTTGATTTTTTTGAAAAAAATGCACCTGCAGAAATTCAGAAATTATTTTCCCGCAATGAATTAATTCAAAAATT
TGCAGAAAAAAAATACCTGTTAAGTTCACCTGAACTAGACACCTTATTAGGTGACGAAAACCACTTAAAGATAAAACAAT
ATTCACTTTTTGAATGGCGTAATTGGATATTGATTCCTAAAGATAAAAAATATTGGATAATAGAAAAAAAAGAAGCCTAA

Upstream 100 bases:

>100_bases
AATCTTTAAAGAAAGAGATTGAAGAAGCTGGCGGTAAAGTTACACTTAAGTAAGAGTACATTTTTTCAAGCCGATAACCT
CAATATCGGCTTCAAAAATT

Downstream 100 bases:

>100_bases
TTTTTTATGAATGAACAGAAGGGTGGATTTAAAAATCTTTATAAAAACTTGATTACCCCTGTATTACAAAAAGACTCTGG
AATTGATGCAGAATACTTAA

Product: ribonuclease HI

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNSDSIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTLEKLKTYKLKENFKLRTDSKY
VIEGYTKWIINWKKNGWKTSSGKPVQNLDLWQKIDQLRINGLIMEYVKGHSGDKQNDRVDKIATNYSKGISIESNLKKVE
YSVDFFEKNAPAEIQKLFSRNELIQKFAEKKYLLSSPELDTLLGDENHLKIKQYSLFEWRNWILIPKDKKYWIIEKKEA

Sequences:

>Translated_239_residues
MNSDSIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTLEKLKTYKLKENFKLRTDSKY
VIEGYTKWIINWKKNGWKTSSGKPVQNLDLWQKIDQLRINGLIMEYVKGHSGDKQNDRVDKIATNYSKGISIESNLKKVE
YSVDFFEKNAPAEIQKLFSRNELIQKFAEKKYLLSSPELDTLLGDENHLKIKQYSLFEWRNWILIPKDKKYWIIEKKEA
>Mature_239_residues
MNSDSIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTLEKLKTYKLKENFKLRTDSKY
VIEGYTKWIINWKKNGWKTSSGKPVQNLDLWQKIDQLRINGLIMEYVKGHSGDKQNDRVDKIATNYSKGISIESNLKKVE
YSVDFFEKNAPAEIQKLFSRNELIQKFAEKKYLLSSPELDTLLGDENHLKIKQYSLFEWRNWILIPKDKKYWIIEKKEA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=141, Percent_Identity=36.1702127659575, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1786408, Length=154, Percent_Identity=45.4545454545455, Blast_Score=131, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI115532542, Length=136, Percent_Identity=38.9705882352941, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17505482, Length=129, Percent_Identity=37.984496124031, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17137476, Length=137, Percent_Identity=32.1167883211679, Blast_Score=67, Evalue=8e-12,
Organism=Drosophila melanogaster, GI45552509, Length=137, Percent_Identity=32.1167883211679, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 27461; Mature: 27461

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDSIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTL
CCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHH
EKLKTYKLKENFKLRTDSKYVIEGYTKWIINWKKNGWKTSSGKPVQNLDLWQKIDQLRIN
HHHHHHHHCCCCEEECCCEEEEECEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHH
GLIMEYVKGHSGDKQNDRVDKIATNYSKGISIESNLKKVEYSVDFFEKNAPAEIQKLFSR
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEHHCCCCCHHHHHHHHH
NELIQKFAEKKYLLSSPELDTLLGDENHLKIKQYSLFEWRNWILIPKDKKYWIIEKKEA
HHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEEEEEECCCEEEEECCCEEEEEEECCC
>Mature Secondary Structure
MNSDSIAIEAATDGACSGNPGPGGWGGLIIFDDNSELEIGGSEQNTTNNRMELTAAIKTL
CCCCCEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEECCCCCCCCCCHHHHHHHHHHH
EKLKTYKLKENFKLRTDSKYVIEGYTKWIINWKKNGWKTSSGKPVQNLDLWQKIDQLRIN
HHHHHHHHCCCCEEECCCEEEEECEEEEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHH
GLIMEYVKGHSGDKQNDRVDKIATNYSKGISIESNLKKVEYSVDFFEKNAPAEIQKLFSR
HHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEEEEHHCCCCCHHHHHHHHH
NELIQKFAEKKYLLSSPELDTLLGDENHLKIKQYSLFEWRNWILIPKDKKYWIIEKKEA
HHHHHHHHHHHHCCCCCCHHHHCCCCCCEEEEEEEEEECCCEEEEECCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917486 [H]