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The map label for this gene is mrdA [H]

Identifier: 123967578

GI number: 123967578

Start: 40286

End: 41932

Strand: Direct

Name: mrdA [H]

Synonym: A9601_00411

Alternate gene names: 123967578

Gene position: 40286-41932 (Clockwise)

Preceding gene: 123967577

Following gene: 123967582

Centisome position: 2.41

GC content: 33.15

Gene sequence:

>1647_bases
ATGTCAGATGATAATAGGATCAGACTTATTGCTTCACAGCCAATACGTGGAAGAATACTAGATAAGAATGGTTATGTTTT
AGCAGATAGTAGAGTTAAATATTCTTTAATAATAAAGCCTCAATCTGTTAATAAAAGCAATTGGGAAAACCATAAATTAA
GTATTTCTAACTTGTTAGATATTGACAGTAATGTAATTCAAGAAAAATATTCTGATGGTCTAAAAAATCAAAAACTCTCA
GTAACTATTCTTGATGATTTAAATGTAAAACAATTAATAAAATTCAAGGAAAATGAAGGTAATTTAAATAGTTTTGAAAT
AGCAACCAAATTAATTAGAAATTACCCTTATAAGTCCCTTGCTGCCCATGTAATTGGATATACTCAGCCAATTACTGAAT
CAGAATATAAATTATTATCTAAAAAGGGTTATAAATTAAACGACTTAATAGGAAGAACAGGAATTGAATATGTTTACGAA
GACTTTATAAGAGGTGAATGGGGTGGCGAAATGGTTGAAGTAAATTCATTAGGAAAATTTAAAAGATCGTTGGGTATAAA
ATCTTCAATAAAAGGTAATGATGTTGAATTGACAATCGATATAAATTTGCAATTAGTTGCTGAGGAAGTTCTTAAAGATA
AAAAAGCAGGAGCGATAATAGTAATGGATCCCAGAGATGGTTCAATAAGGGCGATGGCAAGTAAGCCTACCTTTGATTTG
AATTTTTTTTCAAAGGACTTTAAGCCTGAAAAAGAATACAATAAGATATTTAATTCTCCAGAAAAACCTCTTTTTAATAG
AGCATTAAATGCTTATGATCCAGGAAGTGTATGGAAAATTGTAACTGCTTTAGCGGGCTTAGAAAGTGGTAAATTTCCTA
GAAATACCATGTTAGATACGAAACCATGTATAACTTATGGAAGCCAATGTTTTAGAGAGCACAATGATTTAGGTTTTGGC
GTGATAGGTTATGAAGATGCTTTAAGAGTTTCGAGTAATACATTTTTTTATCAAGTCGGATATGGAGTAGGAGTTGATGA
AATTCATAAGGTCTCCCGAAAACTTGGCTTTAATTCTTTATCCGGAATTGAAATTTCTGAACAAGAAAATATAGGATTAG
TAGCTAGTAGTGAATGGGCAAAAAAAGGTAGAGGATGGAGCGAACCTGGAAGAACTCCATGGATTCCAGAAGATATTGCG
AGTATGTCTATTGGACAATTTGTCGTTCAAGTAACTCCGATGCAAATAGCTAGAGCATATGCTGCAATCGCAAATGGAGG
TTATCTAGTTACTCCACATTTGACTAAAAAAGATGTAGAAAGTCGTTTTGATAAAAAACGTATTCTAATTGACGTTGATC
CACAAAATATTCAATTGATAAAAAGTGGTCTCAGGAAAGTAGTAGAGTCTGGTACAGGGGTATCAATTAATTATGGAGTT
TCAAATTTACCTCCGGTGTCAGGTAAAACAGGAACTGCTGAAGATGGTGAAGGTGGCTTAGATCATGCTTGGTTCGTTTG
CTTTGCTCCTTCAGAAAAGAGTGAGTTGCTTGTAGTTGCCTTTGCACAAAATACTCCTGGTGGAGGCTCGGTGCATGCGC
TTCCAATGGCTAAACAAATATTAAAAGTTTGGAATGAAAAAAAATGA

Upstream 100 bases:

>100_bases
ACAACCTTTAGTCTTACTTATTTTTTCTTCTATTTCGTTTTTATTGATTCTATTTAGATTAATTTTTTTACAACTTTTAA
ATTATGAATCTTTTAAGAAG

Downstream 100 bases:

>100_bases
TAAGAATTTTTTGTTTAAAAGTTTATGTTTTTAATTTTTTCATTTGTAAAAGTCTTTGAATAATATCTTCAATAGTTTTT
GTATCTATAGGTTTACTATA

Product: putative penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSDDNRIRLIASQPIRGRILDKNGYVLADSRVKYSLIIKPQSVNKSNWENHKLSISNLLDIDSNVIQEKYSDGLKNQKLS
VTILDDLNVKQLIKFKENEGNLNSFEIATKLIRNYPYKSLAAHVIGYTQPITESEYKLLSKKGYKLNDLIGRTGIEYVYE
DFIRGEWGGEMVEVNSLGKFKRSLGIKSSIKGNDVELTIDINLQLVAEEVLKDKKAGAIIVMDPRDGSIRAMASKPTFDL
NFFSKDFKPEKEYNKIFNSPEKPLFNRALNAYDPGSVWKIVTALAGLESGKFPRNTMLDTKPCITYGSQCFREHNDLGFG
VIGYEDALRVSSNTFFYQVGYGVGVDEIHKVSRKLGFNSLSGIEISEQENIGLVASSEWAKKGRGWSEPGRTPWIPEDIA
SMSIGQFVVQVTPMQIARAYAAIANGGYLVTPHLTKKDVESRFDKKRILIDVDPQNIQLIKSGLRKVVESGTGVSINYGV
SNLPPVSGKTGTAEDGEGGLDHAWFVCFAPSEKSELLVVAFAQNTPGGGSVHALPMAKQILKVWNEKK

Sequences:

>Translated_548_residues
MSDDNRIRLIASQPIRGRILDKNGYVLADSRVKYSLIIKPQSVNKSNWENHKLSISNLLDIDSNVIQEKYSDGLKNQKLS
VTILDDLNVKQLIKFKENEGNLNSFEIATKLIRNYPYKSLAAHVIGYTQPITESEYKLLSKKGYKLNDLIGRTGIEYVYE
DFIRGEWGGEMVEVNSLGKFKRSLGIKSSIKGNDVELTIDINLQLVAEEVLKDKKAGAIIVMDPRDGSIRAMASKPTFDL
NFFSKDFKPEKEYNKIFNSPEKPLFNRALNAYDPGSVWKIVTALAGLESGKFPRNTMLDTKPCITYGSQCFREHNDLGFG
VIGYEDALRVSSNTFFYQVGYGVGVDEIHKVSRKLGFNSLSGIEISEQENIGLVASSEWAKKGRGWSEPGRTPWIPEDIA
SMSIGQFVVQVTPMQIARAYAAIANGGYLVTPHLTKKDVESRFDKKRILIDVDPQNIQLIKSGLRKVVESGTGVSINYGV
SNLPPVSGKTGTAEDGEGGLDHAWFVCFAPSEKSELLVVAFAQNTPGGGSVHALPMAKQILKVWNEKK
>Mature_547_residues
SDDNRIRLIASQPIRGRILDKNGYVLADSRVKYSLIIKPQSVNKSNWENHKLSISNLLDIDSNVIQEKYSDGLKNQKLSV
TILDDLNVKQLIKFKENEGNLNSFEIATKLIRNYPYKSLAAHVIGYTQPITESEYKLLSKKGYKLNDLIGRTGIEYVYED
FIRGEWGGEMVEVNSLGKFKRSLGIKSSIKGNDVELTIDINLQLVAEEVLKDKKAGAIIVMDPRDGSIRAMASKPTFDLN
FFSKDFKPEKEYNKIFNSPEKPLFNRALNAYDPGSVWKIVTALAGLESGKFPRNTMLDTKPCITYGSQCFREHNDLGFGV
IGYEDALRVSSNTFFYQVGYGVGVDEIHKVSRKLGFNSLSGIEISEQENIGLVASSEWAKKGRGWSEPGRTPWIPEDIAS
MSIGQFVVQVTPMQIARAYAAIANGGYLVTPHLTKKDVESRFDKKRILIDVDPQNIQLIKSGLRKVVESGTGVSINYGVS
NLPPVSGKTGTAEDGEGGLDHAWFVCFAPSEKSELLVVAFAQNTPGGGSVHALPMAKQILKVWNEKK

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=571, Percent_Identity=30.2977232924694, Blast_Score=231, Evalue=1e-61,
Organism=Escherichia coli, GI1786272, Length=453, Percent_Identity=22.075055187638, Blast_Score=76, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 60889; Mature: 60758

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDNRIRLIASQPIRGRILDKNGYVLADSRVKYSLIIKPQSVNKSNWENHKLSISNLLD
CCCCCEEEEEECCCCCCEEECCCCEEEECCCEEEEEEEECCCCCCCCCCCCEEEHHHHHH
IDSNVIQEKYSDGLKNQKLSVTILDDLNVKQLIKFKENEGNLNSFEIATKLIRNYPYKSL
CCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHH
AAHVIGYTQPITESEYKLLSKKGYKLNDLIGRTGIEYVYEDFIRGEWGGEMVEVNSLGKF
HHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHH
KRSLGIKSSIKGNDVELTIDINLQLVAEEVLKDKKAGAIIVMDPRDGSIRAMASKPTFDL
HHHCCCCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCEEE
NFFSKDFKPEKEYNKIFNSPEKPLFNRALNAYDPGSVWKIVTALAGLESGKFPRNTMLDT
ECCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
KPCITYGSQCFREHNDLGFGVIGYEDALRVSSNTFFYQVGYGVGVDEIHKVSRKLGFNSL
CHHHHHHHHHHHHCCCCCEEEECCHHHEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCC
SGIEISEQENIGLVASSEWAKKGRGWSEPGRTPWIPEDIASMSIGQFVVQVTPMQIARAY
CCCEECCCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECHHHHHHHH
AAIANGGYLVTPHLTKKDVESRFDKKRILIDVDPQNIQLIKSGLRKVVESGTGVSINYGV
HHHCCCCEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCC
SNLPPVSGKTGTAEDGEGGLDHAWFVCFAPSEKSELLVVAFAQNTPGGGSVHALPMAKQI
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEHHHHHHHH
LKVWNEKK
HHHHCCCC
>Mature Secondary Structure 
SDDNRIRLIASQPIRGRILDKNGYVLADSRVKYSLIIKPQSVNKSNWENHKLSISNLLD
CCCCEEEEEECCCCCCEEECCCCEEEECCCEEEEEEEECCCCCCCCCCCCEEEHHHHHH
IDSNVIQEKYSDGLKNQKLSVTILDDLNVKQLIKFKENEGNLNSFEIATKLIRNYPYKSL
CCHHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHH
AAHVIGYTQPITESEYKLLSKKGYKLNDLIGRTGIEYVYEDFIRGEWGGEMVEVNSLGKF
HHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEECHHHHH
KRSLGIKSSIKGNDVELTIDINLQLVAEEVLKDKKAGAIIVMDPRDGSIRAMASKPTFDL
HHHCCCCCCCCCCCEEEEEEECHHHHHHHHHCCCCCCEEEEECCCCCCEEEEECCCCEEE
NFFSKDFKPEKEYNKIFNSPEKPLFNRALNAYDPGSVWKIVTALAGLESGKFPRNTMLDT
ECCCCCCCCCHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCC
KPCITYGSQCFREHNDLGFGVIGYEDALRVSSNTFFYQVGYGVGVDEIHKVSRKLGFNSL
CHHHHHHHHHHHHCCCCCEEEECCHHHEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCC
SGIEISEQENIGLVASSEWAKKGRGWSEPGRTPWIPEDIASMSIGQFVVQVTPMQIARAY
CCCEECCCCCCEEEECCHHHHCCCCCCCCCCCCCCCHHHHHHHHHHEEEEECHHHHHHHH
AAIANGGYLVTPHLTKKDVESRFDKKRILIDVDPQNIQLIKSGLRKVVESGTGVSINYGV
HHHCCCCEEEECCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEECCC
SNLPPVSGKTGTAEDGEGGLDHAWFVCFAPSEKSELLVVAFAQNTPGGGSVHALPMAKQI
CCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEEECCCCCCCCEEHHHHHHHH
LKVWNEKK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]