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The map label for this gene is def

Identifier: 121999101

GI number: 121999101

Start: 2548767

End: 2549276

Strand: Reverse

Name: def

Synonym: Hhal_2323

Alternate gene names: 121999101

Gene position: 2549276-2548767 (Counterclockwise)

Preceding gene: 121999105

Following gene: 121999100

Centisome position: 95.18

GC content: 69.61

Gene sequence:

>510_bases
ATGGCCCTCCTCGATATCCTTGTCTATCCCGATCCCCGCCTCCGTGAGCGGGCCGAGCCCGTCGCCGAGGTGGACGACGC
CGTCCGCCGCCTGGTGGACGACATGCTTGAGACCATGTACGAGGCCCGCGGCATCGGTCTGGCCGCCACGCAGGTGGGCG
ACCGCCGGCGGGTGGCGGTGATCGATGTGTCCGAGGAGCGTGACGAGCCGCTGGTGCTGATCAATCCGGAGATCCTCGAG
GCCACCGGCGAGGCCAGCGGCGAGGAAGGCTGCCTGTCCATCCCGGGCTACTACGACGATGTGGCACGGGCCACCCGGGT
GCGCTACCGCGCGCTGGACCGCGACGCCCAGCCCATCGAGGGCGAGGCCGAGGGGACGCTGGCCGTCTGCCTGCAGCACG
AGATCGACCATCTGGACGGTCGACTGTTCATCGACTACCTCTCCGAGCTCAAGCGCAAGCGGGTGCGCAAGCGCATGGAG
AAACGCGAGCGGCTCAGCGCGGCGGGCTGA

Upstream 100 bases:

>100_bases
GGTCTGGATGCTCTGTATAAGCATTCCAGATTATGGCTGCCCTCGGCTGCGCGGTGCGTTACAATCGACCCGTCATTTAA
CAACAGGCACAGCATCGGTC

Downstream 100 bases:

>100_bases
TGGCGGCACGGATCGTCTTTGCCGGAACCCCGGATTTTGCGGTGCCCGGCCTCGATGCCCTGGTCGAGGCCGGGGTGAGG
CCCGTGGCGGTCTTCACCCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 169; Mature: 168

Protein sequence:

>169_residues
MALLDILVYPDPRLRERAEPVAEVDDAVRRLVDDMLETMYEARGIGLAATQVGDRRRVAVIDVSEERDEPLVLINPEILE
ATGEASGEEGCLSIPGYYDDVARATRVRYRALDRDAQPIEGEAEGTLAVCLQHEIDHLDGRLFIDYLSELKRKRVRKRME
KRERLSAAG

Sequences:

>Translated_169_residues
MALLDILVYPDPRLRERAEPVAEVDDAVRRLVDDMLETMYEARGIGLAATQVGDRRRVAVIDVSEERDEPLVLINPEILE
ATGEASGEEGCLSIPGYYDDVARATRVRYRALDRDAQPIEGEAEGTLAVCLQHEIDHLDGRLFIDYLSELKRKRVRKRME
KRERLSAAG
>Mature_168_residues
ALLDILVYPDPRLRERAEPVAEVDDAVRRLVDDMLETMYEARGIGLAATQVGDRRRVAVIDVSEERDEPLVLINPEILEA
TGEASGEEGCLSIPGYYDDVARATRVRYRALDRDAQPIEGEAEGTLAVCLQHEIDHLDGRLFIDYLSELKRKRVRKRMEK
RERLSAAG

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=157, Percent_Identity=31.2101910828025, Blast_Score=67, Evalue=7e-12,
Organism=Escherichia coli, GI1789682, Length=167, Percent_Identity=59.8802395209581, Blast_Score=200, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24645728, Length=155, Percent_Identity=34.1935483870968, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19105; Mature: 18974

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLDILVYPDPRLRERAEPVAEVDDAVRRLVDDMLETMYEARGIGLAATQVGDRRRVAV
CCCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCEEEE
IDVSEERDEPLVLINPEILEATGEASGEEGCLSIPGYYDDVARATRVRYRALDRDAQPIE
EECCCCCCCCEEEECHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCC
GEAEGTLAVCLQHEIDHLDGRLFIDYLSELKRKRVRKRMEKRERLSAAG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLDILVYPDPRLRERAEPVAEVDDAVRRLVDDMLETMYEARGIGLAATQVGDRRRVAV
CCEEEEECCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHCCCCCCEEEE
IDVSEERDEPLVLINPEILEATGEASGEEGCLSIPGYYDDVARATRVRYRALDRDAQPIE
EECCCCCCCCEEEECHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCC
GEAEGTLAVCLQHEIDHLDGRLFIDYLSELKRKRVRKRMEKRERLSAAG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA