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The map label for this gene is pntAA [H]

Identifier: 121999055

GI number: 121999055

Start: 2497542

End: 2498681

Strand: Reverse

Name: pntAA [H]

Synonym: Hhal_2276

Alternate gene names: 121999055

Gene position: 2498681-2497542 (Counterclockwise)

Preceding gene: 121999057

Following gene: 121999054

Centisome position: 93.29

GC content: 67.54

Gene sequence:

>1140_bases
ATGGCCATCAAGGTTGCCGTGCCGAAGGAGACCCGGCCCGGCGAGCGGCGTGTCGCGCTCGTCCCTGGGGTCCTGAAGCA
GTTCGGCAAACTCGGCGTAGAGCTGCTCCTGGAGACGGGAGCCGGTGAGGGCGCCGGGTTCGATGATGCCGATTATGAAG
CCGGCGGTGTTCGCATCGTCCAGTCGCGCGACGAGTTGCTCAAGGAGGCCGATGTCCTGCTCAGCGTGCAGCCGCTGAGC
GTCAAGGAGACCGGCCAGCTGAAGGAAGGGGCGGTGGTCATCAGCTTCCTGACGCCCCACGAAGGCGACGAGCGGATCCA
GACGCTGTGCAAGCGCAAGGTGACCTCCTTCGCCATGGAGCTGGTGCCGCGGATCACCCGCGCCCAGTCCATCGACGCCC
TCTCCTCCCAGGCGGCTGCCGCCGGTTATAAGGCCGCGCTCATCGCCGCCGAGCGCTCCCCGCGCTTCTTCCCCATGTTG
ACCACCGCGGCTGGTACCATCCGCCCGGCCAAGGCTGTGGTCGTCGGCGCCGGTGTGGCCGGCCTGCAGGCCATCGCCAC
CTGCCGCCGCCTGGGTGCCCAGGTCGAGGGCTACGATGTGCGCCCCGAGACCAAGGAGCAGGTGGAGTCGCTCGGCGCCA
AGTTCATCGATACCGGCGTTTCGGCCGTCGGTGAGGGCGGGTACGCCCGTGAGCTGACCGCCGAGGAGCAGCAGCAGCAG
GCCGAGGTGCTGGCCAACCATATCGCCAACGCCCACGTGGTGGTGACCACGGCGGCCATCCCCGGTCGGCCGGCGCCGAA
GATCATCGACAAGGCGACGGTCGAGCGCATGGGCGGTGGTACGGTGATTGTCGACATGGCCGCCGAGACCGGTGGCAACT
GCGAGGTCACCAAGGCCGGTAAGGACGTCACCCACAACGGCGTCCTGGTGGTCGGCCCGAAGAATCTCCCCGCCAGCGTC
GCCGTCCACACCAGCGAGATGTACAGCAAGAACCTGTACAACCTGCTCACGCTGATGGTCGAGGAGGGGGACCTGACTCT
GAACTGGGACGACCAGGTGATCGCGGATACCTGTCTGACCCACGACGGCCAGGTTCGCCACGGGGCCACCCGGGAGCGCC
TGGAAGGAGGTAAGTCATGA

Upstream 100 bases:

>100_bases
ATACCCGTGCGCCGTGATCCGGGTCGGGCTTCAGGCCTGCCCGGGCAGTCGTCACGGACCAACTAACGGCTAACAACTGC
CGCAAACGGGGGATCAAACC

Downstream 100 bases:

>100_bases
TCGAGGTCGGCGGGCTCGTCGCACTGTACGTCTTCATGCTCGCGGCGGTGGCTGGTTACGAGGTCATCACCCGGGTGCCG
GTCATCCTGCATACGCCGCT

Product: NAD(P) transhydrogenase subunit alpha

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 379; Mature: 378

Protein sequence:

>379_residues
MAIKVAVPKETRPGERRVALVPGVLKQFGKLGVELLLETGAGEGAGFDDADYEAGGVRIVQSRDELLKEADVLLSVQPLS
VKETGQLKEGAVVISFLTPHEGDERIQTLCKRKVTSFAMELVPRITRAQSIDALSSQAAAAGYKAALIAAERSPRFFPML
TTAAGTIRPAKAVVVGAGVAGLQAIATCRRLGAQVEGYDVRPETKEQVESLGAKFIDTGVSAVGEGGYARELTAEEQQQQ
AEVLANHIANAHVVVTTAAIPGRPAPKIIDKATVERMGGGTVIVDMAAETGGNCEVTKAGKDVTHNGVLVVGPKNLPASV
AVHTSEMYSKNLYNLLTLMVEEGDLTLNWDDQVIADTCLTHDGQVRHGATRERLEGGKS

Sequences:

>Translated_379_residues
MAIKVAVPKETRPGERRVALVPGVLKQFGKLGVELLLETGAGEGAGFDDADYEAGGVRIVQSRDELLKEADVLLSVQPLS
VKETGQLKEGAVVISFLTPHEGDERIQTLCKRKVTSFAMELVPRITRAQSIDALSSQAAAAGYKAALIAAERSPRFFPML
TTAAGTIRPAKAVVVGAGVAGLQAIATCRRLGAQVEGYDVRPETKEQVESLGAKFIDTGVSAVGEGGYARELTAEEQQQQ
AEVLANHIANAHVVVTTAAIPGRPAPKIIDKATVERMGGGTVIVDMAAETGGNCEVTKAGKDVTHNGVLVVGPKNLPASV
AVHTSEMYSKNLYNLLTLMVEEGDLTLNWDDQVIADTCLTHDGQVRHGATRERLEGGKS
>Mature_378_residues
AIKVAVPKETRPGERRVALVPGVLKQFGKLGVELLLETGAGEGAGFDDADYEAGGVRIVQSRDELLKEADVLLSVQPLSV
KETGQLKEGAVVISFLTPHEGDERIQTLCKRKVTSFAMELVPRITRAQSIDALSSQAAAAGYKAALIAAERSPRFFPMLT
TAAGTIRPAKAVVVGAGVAGLQAIATCRRLGAQVEGYDVRPETKEQVESLGAKFIDTGVSAVGEGGYARELTAEEQQQQA
EVLANHIANAHVVVTTAAIPGRPAPKIIDKATVERMGGGTVIVDMAAETGGNCEVTKAGKDVTHNGVLVVGPKNLPASVA
VHTSEMYSKNLYNLLTLMVEEGDLTLNWDDQVIADTCLTHDGQVRHGATRERLEGGKS

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=380, Percent_Identity=38.4210526315789, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI122939153, Length=380, Percent_Identity=38.4210526315789, Blast_Score=240, Evalue=1e-63,
Organism=Escherichia coli, GI1787887, Length=372, Percent_Identity=41.3978494623656, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17550456, Length=366, Percent_Identity=39.8907103825137, Blast_Score=270, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 40009; Mature: 39878

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKVAVPKETRPGERRVALVPGVLKQFGKLGVELLLETGAGEGAGFDDADYEAGGVRIV
CEEEEECCCCCCCCCCEEEECHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCEEEE
QSRDELLKEADVLLSVQPLSVKETGQLKEGAVVISFLTPHEGDERIQTLCKRKVTSFAME
ECHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LVPRITRAQSIDALSSQAAAAGYKAALIAAERSPRFFPMLTTAAGTIRPAKAVVVGAGVA
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEHHCCCCCCCCCEEEEECCHH
GLQAIATCRRLGAQVEGYDVRPETKEQVESLGAKFIDTGVSAVGEGGYARELTAEEQQQQ
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHH
AEVLANHIANAHVVVTTAAIPGRPAPKIIDKATVERMGGGTVIVDMAAETGGNCEVTKAG
HHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECC
KDVTHNGVLVVGPKNLPASVAVHTSEMYSKNLYNLLTLMVEEGDLTLNWDDQVIADTCLT
CCCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHEEECCCEEECCCCHHHHHHHHC
HDGQVRHGATRERLEGGKS
CCCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
AIKVAVPKETRPGERRVALVPGVLKQFGKLGVELLLETGAGEGAGFDDADYEAGGVRIV
EEEEECCCCCCCCCCEEEECHHHHHHHHHHCEEEEEECCCCCCCCCCCCCCCCCCEEEE
QSRDELLKEADVLLSVQPLSVKETGQLKEGAVVISFLTPHEGDERIQTLCKRKVTSFAME
ECHHHHHHHHHHEEEECCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
LVPRITRAQSIDALSSQAAAAGYKAALIAAERSPRFFPMLTTAAGTIRPAKAVVVGAGVA
HHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEHHCCCCCCCCCEEEEECCHH
GLQAIATCRRLGAQVEGYDVRPETKEQVESLGAKFIDTGVSAVGEGGYARELTAEEQQQQ
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHHHH
AEVLANHIANAHVVVTTAAIPGRPAPKIIDKATVERMGGGTVIVDMAAETGGNCEVTKAG
HHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCEEEEECC
KDVTHNGVLVVGPKNLPASVAVHTSEMYSKNLYNLLTLMVEEGDLTLNWDDQVIADTCLT
CCCCCCCEEEECCCCCCCEEEEEHHHHHHHHHHHHHHHEEECCCEEECCCCHHHHHHHHC
HDGQVRHGATRERLEGGKS
CCCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]