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The map label for this gene is mlaE [H]

Identifier: 121998898

GI number: 121998898

Start: 2322191

End: 2322967

Strand: Reverse

Name: mlaE [H]

Synonym: Hhal_2119

Alternate gene names: 121998898

Gene position: 2322967-2322191 (Counterclockwise)

Preceding gene: 121998899

Following gene: 121998897

Centisome position: 86.73

GC content: 66.28

Gene sequence:

>777_bases
ATGATCGGCTTGTTCCAGGCGCTGGGGCAACGGACCCTGACTGCGCTGCGCGGCCTGGGCGAGGCGGTGCTGCTCATGGG
GCGGATCCTGCGCGCCGCCCCCGAGGTGGTGGTGCGCCCCCGCCTGCTCATCGAGCAGGTCTACGCCGTCGGCGTGTTGT
CGCTGGTGATCATCCTGGTGTCGGCGCTGTTCGTCGGTATGGTCCTCGGCCTGCAGGGGTACTACACCCTGGCGGACTTC
GGGGCCACCGAAGCGGTGGGCACCATGGTCGCCCTGTCTCTAGTCCGCGAACTCGGGCCTGTGGTAACCGCGCTGCTGTT
CGCCGGGCGGGCTGGCTCATCGCTGACCGCCGAGGTGGGGCTGATGAAGGCCACCGAGCAGCTCTCGAGCATGGAGATGA
TGGCGGTGGACCCGGAGCGCCGTATTCTGGCCCCGCGCTTCTTCGCTGCCGTATTGGCGGTGCCACTGCTCGCCGCCGTG
TTCAGCGCCGTGGGCGTGTTCGGTGGTTATCTGGTGGCGGTGGGCATGGTCGGGATCGACAGCGGCGCGTTCTGGTCCAA
CATGCAGAACGCAGTCACCATCCAGGATGATATTTTGAATGGCGTGATCAAGAGCGTCGTCTTCGGCTTCTTGGTCGGCT
GGATCGCCCTGCACAAGGGGTACATGGCAGAGCCGACCTCGAGCGGTGTCAGTCGGGCGACGACCTTGGGCGTCGTGCAC
GCTTCGCTGGCGGTGCTCGGGCTCGACTTCCTGCTCACCGCACTGATGTTCGACTGA

Upstream 100 bases:

>100_bases
AGTCGCTGCGCCAGTTTATCGATGCCCGGCCGGATGGTCCGGTGCCGTTCCACTATCCGGCGCCGCGGCTGTCCGACGAA
CTGCTCGGCGGAGGGGGGCG

Downstream 100 bases:

>100_bases
CGGAGCAAGGGTATGAGTCAGCGGCTCGTGGAGATCTGGGTCGGGGTGTTCGTGGCCATTGGCCTGGCGGCGATGATGGT
CCTGGCCCTGCAGGTCAGTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MIGLFQALGQRTLTALRGLGEAVLLMGRILRAAPEVVVRPRLLIEQVYAVGVLSLVIILVSALFVGMVLGLQGYYTLADF
GATEAVGTMVALSLVRELGPVVTALLFAGRAGSSLTAEVGLMKATEQLSSMEMMAVDPERRILAPRFFAAVLAVPLLAAV
FSAVGVFGGYLVAVGMVGIDSGAFWSNMQNAVTIQDDILNGVIKSVVFGFLVGWIALHKGYMAEPTSSGVSRATTLGVVH
ASLAVLGLDFLLTALMFD

Sequences:

>Translated_258_residues
MIGLFQALGQRTLTALRGLGEAVLLMGRILRAAPEVVVRPRLLIEQVYAVGVLSLVIILVSALFVGMVLGLQGYYTLADF
GATEAVGTMVALSLVRELGPVVTALLFAGRAGSSLTAEVGLMKATEQLSSMEMMAVDPERRILAPRFFAAVLAVPLLAAV
FSAVGVFGGYLVAVGMVGIDSGAFWSNMQNAVTIQDDILNGVIKSVVFGFLVGWIALHKGYMAEPTSSGVSRATTLGVVH
ASLAVLGLDFLLTALMFD
>Mature_258_residues
MIGLFQALGQRTLTALRGLGEAVLLMGRILRAAPEVVVRPRLLIEQVYAVGVLSLVIILVSALFVGMVLGLQGYYTLADF
GATEAVGTMVALSLVRELGPVVTALLFAGRAGSSLTAEVGLMKATEQLSSMEMMAVDPERRILAPRFFAAVLAVPLLAAV
FSAVGVFGGYLVAVGMVGIDSGAFWSNMQNAVTIQDDILNGVIKSVVFGFLVGWIALHKGYMAEPTSSGVSRATTLGVVH
ASLAVLGLDFLLTALMFD

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=251, Percent_Identity=61.3545816733068, Blast_Score=297, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27048; Mature: 27048

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIGLFQALGQRTLTALRGLGEAVLLMGRILRAAPEVVVRPRLLIEQVYAVGVLSLVIILV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
SALFVGMVLGLQGYYTLADFGATEAVGTMVALSLVRELGPVVTALLFAGRAGSSLTAEVG
HHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
LMKATEQLSSMEMMAVDPERRILAPRFFAAVLAVPLLAAVFSAVGVFGGYLVAVGMVGID
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SGAFWSNMQNAVTIQDDILNGVIKSVVFGFLVGWIALHKGYMAEPTSSGVSRATTLGVVH
CCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
ASLAVLGLDFLLTALMFD
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]