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The map label for this gene is mraW

Identifier: 121998878

GI number: 121998878

Start: 2304738

End: 2305712

Strand: Reverse

Name: mraW

Synonym: Hhal_2099

Alternate gene names: 121998878

Gene position: 2305712-2304738 (Counterclockwise)

Preceding gene: 121998879

Following gene: 121998877

Centisome position: 86.08

GC content: 73.23

Gene sequence:

>975_bases
ATGTCCGCAGCGGAGATGTGCGCAGTGGCGGAGCAGGAGCCAACGGCGACGACACACCGGCCGGTCCTCTATGGGGCCGC
GCTGGCCGCGCTCGACGTGCGTCCGGACGGCTGCTATGTGGACGCCACCTACGGGCGTGGCGGTCACGCCCGCGGTATCC
TGGAGCGACTGGGCCCCCAAGGGCGGTTGTGGGTGGCCGACCGGGACCCGGAGGCGCTGGCCCACGCCCGCGAGACCCTG
GCGGACGACCCCCGCTGCACCGTGCTGGGTGCCGAACTGGCCGAGCTGCCCCGGCTGTTGGCCGAGCAGGGGCTCGCCGC
CGGGGTCGACGGCCTGCTGGCGGATCTCGGCATCTCCTCGCCTCAGGTCGACAACCCCGACCGCGGTTTCTCGTTCCAGC
GCGATGGGCCGCTGGATATGCGCATGGATCCGACCACCGGCGAGTCCGCCGCAGCGCTCCTGGAGCGTCTGAGTGCGCGC
GACATCGCCGGGGTCCTGCGGCAACTCGGCGAAGAGCGCCACGCCGGGCGGATTGCGCGTGCCATTGTCGCTGCCCGGGA
CGCCGGCGACCCGCCGCGCACCACGCTGGCGCTGGCCCGGCTGGTCGAGCAGGCGGTACCGCGGCGTGAGCCGGGGCGCC
ATCCGGCGACGCGGACCTTCCAGGCGCTGCGTATCGCAGTCAACGATGAACTTGGACAGCTGGACCGCTTCCTGGAGGGT
GTCATCGACCTGTTGGCCCCGGGGGGGCGGCTGGCCGTGATCGCGTTCCACTCGCTGGAGGACCGGCGGGTGAAGCGGTT
CATCCGCCGGGCGTCCAGCGTGGGCGATCTGCCGCCGTCGGTGCCGGTGCCGCCGGCGGGTTGTCAGCCGAGGCTGCGGC
CACTGGGGCGTGATCTCCGTGCCGACGAGGGCGAGGTGGCTGGCAACCCGCGCGCGCGCAGCGCGGTATTGCGGGTCGCG
GAGCGGTTGTCATGA

Upstream 100 bases:

>100_bases
CAGCGGCGGGCCGACTGGCTGCAGGAGGCCGCCGCGGCCGACGCCGAGCTCCCCGGGGAGCTGGAGAGCCTCGCCCTGTA
ACTCAGGTCAGGGGGGCGAC

Downstream 100 bases:

>100_bases
GTCGTGCGGCCTGGGGGGGCATCGCCTTGGTGGTGGCCGTTGTGGTCAGCGCCGTGGGGGTGGTAGCGATTCAGCACGAG
TATCGGGGGGGGTTCATTAC

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 324; Mature: 323

Protein sequence:

>324_residues
MSAAEMCAVAEQEPTATTHRPVLYGAALAALDVRPDGCYVDATYGRGGHARGILERLGPQGRLWVADRDPEALAHARETL
ADDPRCTVLGAELAELPRLLAEQGLAAGVDGLLADLGISSPQVDNPDRGFSFQRDGPLDMRMDPTTGESAAALLERLSAR
DIAGVLRQLGEERHAGRIARAIVAARDAGDPPRTTLALARLVEQAVPRREPGRHPATRTFQALRIAVNDELGQLDRFLEG
VIDLLAPGGRLAVIAFHSLEDRRVKRFIRRASSVGDLPPSVPVPPAGCQPRLRPLGRDLRADEGEVAGNPRARSAVLRVA
ERLS

Sequences:

>Translated_324_residues
MSAAEMCAVAEQEPTATTHRPVLYGAALAALDVRPDGCYVDATYGRGGHARGILERLGPQGRLWVADRDPEALAHARETL
ADDPRCTVLGAELAELPRLLAEQGLAAGVDGLLADLGISSPQVDNPDRGFSFQRDGPLDMRMDPTTGESAAALLERLSAR
DIAGVLRQLGEERHAGRIARAIVAARDAGDPPRTTLALARLVEQAVPRREPGRHPATRTFQALRIAVNDELGQLDRFLEG
VIDLLAPGGRLAVIAFHSLEDRRVKRFIRRASSVGDLPPSVPVPPAGCQPRLRPLGRDLRADEGEVAGNPRARSAVLRVA
ERLS
>Mature_323_residues
SAAEMCAVAEQEPTATTHRPVLYGAALAALDVRPDGCYVDATYGRGGHARGILERLGPQGRLWVADRDPEALAHARETLA
DDPRCTVLGAELAELPRLLAEQGLAAGVDGLLADLGISSPQVDNPDRGFSFQRDGPLDMRMDPTTGESAAALLERLSARD
IAGVLRQLGEERHAGRIARAIVAARDAGDPPRTTLALARLVEQAVPRREPGRHPATRTFQALRIAVNDELGQLDRFLEGV
IDLLAPGGRLAVIAFHSLEDRRVKRFIRRASSVGDLPPSVPVPPAGCQPRLRPLGRDLRADEGEVAGNPRARSAVLRVAE
RLS

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=350, Percent_Identity=37.4285714285714, Blast_Score=172, Evalue=3e-43,
Organism=Homo sapiens, GI165377202, Length=201, Percent_Identity=39.3034825870647, Blast_Score=112, Evalue=4e-25,
Organism=Escherichia coli, GI1786270, Length=313, Percent_Identity=51.1182108626198, Blast_Score=291, Evalue=4e-80,
Organism=Drosophila melanogaster, GI62472493, Length=335, Percent_Identity=39.4029850746269, Blast_Score=187, Evalue=7e-48,
Organism=Drosophila melanogaster, GI28571637, Length=335, Percent_Identity=39.4029850746269, Blast_Score=187, Evalue=7e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_HALHL (A1WYV1)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003665.1
- ProteinModelPortal:   A1WYV1
- SMR:   A1WYV1
- STRING:   A1WYV1
- GeneID:   4709953
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_2099
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   RPINSTL
- PhylomeDB:   A1WYV1
- ProtClustDB:   PRK00050
- BioCyc:   HHAL349124:HHAL_2099-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34729; Mature: 34598

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: BINDING 67-67 BINDING 96-96 BINDING 115-115 BINDING 122-122

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAEMCAVAEQEPTATTHRPVLYGAALAALDVRPDGCYVDATYGRGGHARGILERLGPQ
CCHHHHHHHHCCCCCCCCCCCHHHHHHHHHEECCCCCEEEECCCCCCCHHHHHHHHCCCC
GRLWVADRDPEALAHARETLADDPRCTVLGAELAELPRLLAEQGLAAGVDGLLADLGISS
CEEEEECCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
PQVDNPDRGFSFQRDGPLDMRMDPTTGESAAALLERLSARDIAGVLRQLGEERHAGRIAR
CCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVAARDAGDPPRTTLALARLVEQAVPRREPGRHPATRTFQALRIAVNDELGQLDRFLEG
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VIDLLAPGGRLAVIAFHSLEDRRVKRFIRRASSVGDLPPSVPVPPAGCQPRLRPLGRDLR
HHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCC
ADEGEVAGNPRARSAVLRVAERLS
CCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAAEMCAVAEQEPTATTHRPVLYGAALAALDVRPDGCYVDATYGRGGHARGILERLGPQ
CHHHHHHHHCCCCCCCCCCCHHHHHHHHHEECCCCCEEEECCCCCCCHHHHHHHHCCCC
GRLWVADRDPEALAHARETLADDPRCTVLGAELAELPRLLAEQGLAAGVDGLLADLGISS
CEEEEECCCHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
PQVDNPDRGFSFQRDGPLDMRMDPTTGESAAALLERLSARDIAGVLRQLGEERHAGRIAR
CCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AIVAARDAGDPPRTTLALARLVEQAVPRREPGRHPATRTFQALRIAVNDELGQLDRFLEG
HHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VIDLLAPGGRLAVIAFHSLEDRRVKRFIRRASSVGDLPPSVPVPPAGCQPRLRPLGRDLR
HHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHCCCCC
ADEGEVAGNPRARSAVLRVAERLS
CCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA