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The map label for this gene is thiE

Identifier: 121998791

GI number: 121998791

Start: 2216579

End: 2217208

Strand: Reverse

Name: thiE

Synonym: Hhal_2012

Alternate gene names: 121998791

Gene position: 2217208-2216579 (Counterclockwise)

Preceding gene: 121998792

Following gene: 121998790

Centisome position: 82.78

GC content: 72.06

Gene sequence:

>630_bases
GTGAACAAGCAACCGAGTCAACTGGCCGGGGTCTATGCGGTGACCCAGCCCCGCCCCGATCTTCAGGAGGCCGTGGCGGC
GGTCCTGCGGGGCGGCGTGGGTATCGTCCAGTACCGCGACAAGAGTGAGGATGCCGACCGCCGTCGCGAGGAGGCGGGCG
CCCTGTGCCGGCTGTGTGAGGAGTACGGCGCCCTGTTCCTGGTCAACGACGATGTGGACCTGGCCGCGGCCGTGGCGGCC
CACGGTGTGCATCTGGGCCGCGACGACGGGGCCGTGGTGGCGGCCCGGCAGCAGCTGGGCGACACCGCCTGGATCGGTGT
GTCGTGCTACGACGATCTGGATCGCGCCCGTCGGTTGGTGGCCGAGGGCGCCGACTACGTGGCCTTCGGCAGCATCTTCC
CGTCGCCAACCAAGCCCGAATCCGGGCTCGCGCCCATGGAGCTTCTGCGCAGCGGGCGCGAGGCCACGGGCTGCCCGACG
GTGGCCATCGGCGGCATCGACGCCGGCAATATCCATGAGGTGGCTGCCGCCGGGGCCGATGCCGCCGCCGTGGTCAGTGC
CCTGTTCGCCGCCGAGGATCCGGAGGCGGCCGCCCGGCAGCTCGTCGCCCAGTGGCAGCGCAGTCGGTAG

Upstream 100 bases:

>100_bases
GAGCCGGGCACCCCGTTCGAGGACTTGCCGGAGGAATTCCTCTGCCCGGAGTGCAGCGCCGGCAAGGAGGCCTTCGAGGT
GATGGAGGTTTGAGGTCGAA

Downstream 100 bases:

>100_bases
AATCCCGTCCTGATACCGAATCACAAGACAAGAGGAGTACCCATGCAGCGCACCCATCAGTTATTCCAGCAGGCCCGGGA
GCTGATCCCCGGGGGCGTCA

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MNKQPSQLAGVYAVTQPRPDLQEAVAAVLRGGVGIVQYRDKSEDADRRREEAGALCRLCEEYGALFLVNDDVDLAAAVAA
HGVHLGRDDGAVVAARQQLGDTAWIGVSCYDDLDRARRLVAEGADYVAFGSIFPSPTKPESGLAPMELLRSGREATGCPT
VAIGGIDAGNIHEVAAAGADAAAVVSALFAAEDPEAAARQLVAQWQRSR

Sequences:

>Translated_209_residues
MNKQPSQLAGVYAVTQPRPDLQEAVAAVLRGGVGIVQYRDKSEDADRRREEAGALCRLCEEYGALFLVNDDVDLAAAVAA
HGVHLGRDDGAVVAARQQLGDTAWIGVSCYDDLDRARRLVAEGADYVAFGSIFPSPTKPESGLAPMELLRSGREATGCPT
VAIGGIDAGNIHEVAAAGADAAAVVSALFAAEDPEAAARQLVAQWQRSR
>Mature_209_residues
MNKQPSQLAGVYAVTQPRPDLQEAVAAVLRGGVGIVQYRDKSEDADRRREEAGALCRLCEEYGALFLVNDDVDLAAAVAA
HGVHLGRDDGAVVAARQQLGDTAWIGVSCYDDLDRARRLVAEGADYVAFGSIFPSPTKPESGLAPMELLRSGREATGCPT
VAIGGIDAGNIHEVAAAGADAAAVVSALFAAEDPEAAARQLVAQWQRSR

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=206, Percent_Identity=34.9514563106796, Blast_Score=74, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6325042, Length=215, Percent_Identity=31.1627906976744, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_HALHL (A1WYL4)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003578.1
- ProteinModelPortal:   A1WYL4
- SMR:   A1WYL4
- STRING:   A1WYL4
- GeneID:   4710409
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_2012
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   IMGSNNC
- BioCyc:   HHAL349124:HHAL_2012-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 21913; Mature: 21913

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQPSQLAGVYAVTQPRPDLQEAVAAVLRGGVGIVQYRDKSEDADRRREEAGALCRLCE
CCCCCCHHHCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
EYGALFLVNDDVDLAAAVAAHGVHLGRDDGAVVAARQQLGDTAWIGVSCYDDLDRARRLV
HHCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCEEEEEEHHHHHHHHHHHHH
AEGADYVAFGSIFPSPTKPESGLAPMELLRSGREATGCPTVAIGGIDAGNIHEVAAAGAD
HCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHCCCC
AAAVVSALFAAEDPEAAARQLVAQWQRSR
HHHHHHHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKQPSQLAGVYAVTQPRPDLQEAVAAVLRGGVGIVQYRDKSEDADRRREEAGALCRLCE
CCCCCCHHHCEEEECCCCCCHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHH
EYGALFLVNDDVDLAAAVAAHGVHLGRDDGAVVAARQQLGDTAWIGVSCYDDLDRARRLV
HHCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEHHHHHCCCEEEEEEHHHHHHHHHHHHH
AEGADYVAFGSIFPSPTKPESGLAPMELLRSGREATGCPTVAIGGIDAGNIHEVAAAGAD
HCCCCEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHCCCC
AAAVVSALFAAEDPEAAARQLVAQWQRSR
HHHHHHHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA