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The map label for this gene is ispB [H]

Identifier: 121998628

GI number: 121998628

Start: 2020751

End: 2021722

Strand: Reverse

Name: ispB [H]

Synonym: Hhal_1849

Alternate gene names: 121998628

Gene position: 2021722-2020751 (Counterclockwise)

Preceding gene: 121998629

Following gene: 121998627

Centisome position: 75.48

GC content: 64.61

Gene sequence:

>972_bases
ATGAACCTTGACGCAATCCGTGAGCTCGCCGCTGACGACATGGCGGAGGTCAACCGCCTGATCGAGGAGCGGCTTTCCTC
CGACGTGGTGCTGATCAATCAGCTCGCCTACCACATCGTCCATAGTGGCGGTAAGCGGCTGCGCCCCATGGTTGTGCTCC
TGGCGGCGCGGGCGTGCGGGTACCAAGGTAAGGATCACGTCCAGCTGGCGGCGATCATCGAGTTCATCCACACTGCGACG
CTGCTCCATGACGACGTGGTGGACGGCTCGGACATGCGCCGCGGCAAGCAGACGGCGCACACCATCTGGGGGAACGAGGC
CAGCGTCCTGGTCGGCGACTTCCTCTACACTCGCGCCTTCGAGATGATGGTCGAGCTCGACTCGATGGCCGTGCTGCGGA
TTCTGGCGCGGGCGACCAACACCATCGCTCGGGGTGAGGTGATGCAGCTGATGAACGTCCACGAGCCGGACCTGACCGAG
GCGGATTACCGCCAGGTGATCTACCGCAAGACGGCGACCCTCTTCGAGGCGGGGTGCCAGAACGGAGCCGTGCTCGCCGG
GCTGGACGACGCCGGCTGCGAAGCGCTGGCGGCCTACGGGCGGCATCTGGGTACGGCGTACCAGCTGGTGGACGATGCCC
TCGATTACAGTGGCGATGCCAGCGAGATCGGCAAGAATATCGGCGACGATCTGGCCGAGGGCAAGACCACCATGCCGCTG
ATCCACGCCATGCGTGAGGGTGAGCCCGCCGATCGCGACCTGATTCGCCAGGCCATTGAGGCCGGTGGGCTGGATCAGAT
GGAAGCCGTCGGTCGGGCGATTGAAAAGACCGGGTCGGCTGAATATACTAGGCGCCTCGCTGAGGTTGAGGTTGAGCAGG
CCGTCGAGCAGCTTACCTCGGCGGTTCCGGGTAGCCCCTGGCGAGATGCGCTGGTACACTTGGCGCGCAACTCGATATCC
CGGACAGCGTAA

Upstream 100 bases:

>100_bases
ATCTGGCGCAGTGGCCGCCGCGGCCGGGGAGGCCGTGCTTGACACCCTCGGCCCCCCTGCCTAGCATCCGCGGGGCTGAC
CCGGAGCGCAGATCCTGCCC

Downstream 100 bases:

>100_bases
CAGGTTGCATACGGGGTGTAGCTCAGTTTGGTAGAGCACTGCCTTCGGGAGGCAGGGGCCGCAGGTTCAAATCCTGCCAC
CCCGACCAGACAATCCAAGG

Product: trans-hexaprenyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNLDAIRELAADDMAEVNRLIEERLSSDVVLINQLAYHIVHSGGKRLRPMVVLLAARACGYQGKDHVQLAAIIEFIHTAT
LLHDDVVDGSDMRRGKQTAHTIWGNEASVLVGDFLYTRAFEMMVELDSMAVLRILARATNTIARGEVMQLMNVHEPDLTE
ADYRQVIYRKTATLFEAGCQNGAVLAGLDDAGCEALAAYGRHLGTAYQLVDDALDYSGDASEIGKNIGDDLAEGKTTMPL
IHAMREGEPADRDLIRQAIEAGGLDQMEAVGRAIEKTGSAEYTRRLAEVEVEQAVEQLTSAVPGSPWRDALVHLARNSIS
RTA

Sequences:

>Translated_323_residues
MNLDAIRELAADDMAEVNRLIEERLSSDVVLINQLAYHIVHSGGKRLRPMVVLLAARACGYQGKDHVQLAAIIEFIHTAT
LLHDDVVDGSDMRRGKQTAHTIWGNEASVLVGDFLYTRAFEMMVELDSMAVLRILARATNTIARGEVMQLMNVHEPDLTE
ADYRQVIYRKTATLFEAGCQNGAVLAGLDDAGCEALAAYGRHLGTAYQLVDDALDYSGDASEIGKNIGDDLAEGKTTMPL
IHAMREGEPADRDLIRQAIEAGGLDQMEAVGRAIEKTGSAEYTRRLAEVEVEQAVEQLTSAVPGSPWRDALVHLARNSIS
RTA
>Mature_323_residues
MNLDAIRELAADDMAEVNRLIEERLSSDVVLINQLAYHIVHSGGKRLRPMVVLLAARACGYQGKDHVQLAAIIEFIHTAT
LLHDDVVDGSDMRRGKQTAHTIWGNEASVLVGDFLYTRAFEMMVELDSMAVLRILARATNTIARGEVMQLMNVHEPDLTE
ADYRQVIYRKTATLFEAGCQNGAVLAGLDDAGCEALAAYGRHLGTAYQLVDDALDYSGDASEIGKNIGDDLAEGKTTMPL
IHAMREGEPADRDLIRQAIEAGGLDQMEAVGRAIEKTGSAEYTRRLAEVEVEQAVEQLTSAVPGSPWRDALVHLARNSIS
RTA

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=297, Percent_Identity=30.976430976431, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI4758430, Length=252, Percent_Identity=30.5555555555556, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI83700220, Length=252, Percent_Identity=30.5555555555556, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=57.4534161490683, Blast_Score=369, Evalue=1e-103,
Organism=Escherichia coli, GI1786623, Length=239, Percent_Identity=33.0543933054393, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17505681, Length=348, Percent_Identity=29.0229885057471, Blast_Score=155, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6319475, Length=298, Percent_Identity=30.5369127516779, Blast_Score=129, Evalue=6e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=243, Percent_Identity=25.1028806584362, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=31.8791946308725, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI281365769, Length=295, Percent_Identity=26.7796610169492, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24660002, Length=295, Percent_Identity=26.7796610169492, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35269; Mature: 35269

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLDAIRELAADDMAEVNRLIEERLSSDVVLINQLAYHIVHSGGKRLRPMVVLLAARACG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
YQGKDHVQLAAIIEFIHTATLLHDDVVDGSDMRRGKQTAHTIWGNEASVLVGDFLYTRAF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EMMVELDSMAVLRILARATNTIARGEVMQLMNVHEPDLTEADYRQVIYRKTATLFEAGCQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NGAVLAGLDDAGCEALAAYGRHLGTAYQLVDDALDYSGDASEIGKNIGDDLAEGKTTMPL
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHH
IHAMREGEPADRDLIRQAIEAGGLDQMEAVGRAIEKTGSAEYTRRLAEVEVEQAVEQLTS
HHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AVPGSPWRDALVHLARNSISRTA
HCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNLDAIRELAADDMAEVNRLIEERLSSDVVLINQLAYHIVHSGGKRLRPMVVLLAARACG
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHC
YQGKDHVQLAAIIEFIHTATLLHDDVVDGSDMRRGKQTAHTIWGNEASVLVGDFLYTRAF
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
EMMVELDSMAVLRILARATNTIARGEVMQLMNVHEPDLTEADYRQVIYRKTATLFEAGCQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCC
NGAVLAGLDDAGCEALAAYGRHLGTAYQLVDDALDYSGDASEIGKNIGDDLAEGKTTMPL
CCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHH
IHAMREGEPADRDLIRQAIEAGGLDQMEAVGRAIEKTGSAEYTRRLAEVEVEQAVEQLTS
HHHHHCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AVPGSPWRDALVHLARNSISRTA
HCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]