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The map label for this gene is trmJ [H]

Identifier: 121998574

GI number: 121998574

Start: 1967272

End: 1968021

Strand: Reverse

Name: trmJ [H]

Synonym: Hhal_1795

Alternate gene names: 121998574

Gene position: 1968021-1967272 (Counterclockwise)

Preceding gene: 121998576

Following gene: 121998573

Centisome position: 73.48

GC content: 69.6

Gene sequence:

>750_bases
TTGAGCCTTGAACGCACACGTATTGTGTTGGTCGGGACCACCCACCCCGGCAACATCGGCGCGGCTGCGCGAGCCATGCG
CACCATGGGACTGCGGGACCTCTACCTGGTGGACCCGCAGTGTGACCCGGGCGACCCGGAGGCCGTGGCCCGGGCCGCCG
GCGCGGGCGATGTCCTGGAGCGGGCGCGGACTGCTCCGGACCTTGCCACGGCCCTGACCGGCTGTCGCCTGGCCTTTGGG
CTGTCGGCGCGCAGTCGTGCCGAGCGGCAGCCTCGGCTGGAGTTGCGCCCGGCCGCCGAGTCCGCTGTCGCCCAGGGGGG
TGACGGTGACCTGGCGTGGGTGTTCGGGCGGGAGCGCACCGGCCTGACCAACGCCGAACTCGACCATTGCCACTACCTGG
TGCAGATCCCCACGGATCCGGATTACAGTTCCCTGAATGTGGCGCAGTCGGTGCAGCTGGTAGCGTGGGAGTTGCGCATG
GCGGCCGCGGCCGGGGGGCCGGTGCAGGGCCAGGCGGAGGAGCCGCCCGCGGCGGTGGATACGCTGGAGCGTTTCTTCGA
TCACCTGGAGGAGATGGCCGGTCGGGTGGGTTATCTCGACCGGCACAACCCCGCGCTGACGATGCGGCGGCTGCGTAACA
TCTTTCGCCGCGCATGGCTGACCGAGGATGAGGTACGGATGCTGCGCGGTCTGATCAGCCACGTACTCAACCCGCGCCGC
TGGCGCGAGTCCAATTGTCGTAAAGGGTAG

Upstream 100 bases:

>100_bases
GGACTGCGATGTTGACCATTGGATGCATCGGGAAAGCTCCTACGCTGGACGCGCTACAGGATAACAGCAAAACTGATGCG
ATTCAGACCGGGAGGCCATC

Downstream 100 bases:

>100_bases
ATGGATGCTGCGACGCCTGCGTGAGGATATCCGCTGTGTCATGGATCGCGATCCGGCCGCTCGGAACGCCTTCCATGTGG
TGACCACCTATCCCGGTTTC

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MSLERTRIVLVGTTHPGNIGAAARAMRTMGLRDLYLVDPQCDPGDPEAVARAAGAGDVLERARTAPDLATALTGCRLAFG
LSARSRAERQPRLELRPAAESAVAQGGDGDLAWVFGRERTGLTNAELDHCHYLVQIPTDPDYSSLNVAQSVQLVAWELRM
AAAAGGPVQGQAEEPPAAVDTLERFFDHLEEMAGRVGYLDRHNPALTMRRLRNIFRRAWLTEDEVRMLRGLISHVLNPRR
WRESNCRKG

Sequences:

>Translated_249_residues
MSLERTRIVLVGTTHPGNIGAAARAMRTMGLRDLYLVDPQCDPGDPEAVARAAGAGDVLERARTAPDLATALTGCRLAFG
LSARSRAERQPRLELRPAAESAVAQGGDGDLAWVFGRERTGLTNAELDHCHYLVQIPTDPDYSSLNVAQSVQLVAWELRM
AAAAGGPVQGQAEEPPAAVDTLERFFDHLEEMAGRVGYLDRHNPALTMRRLRNIFRRAWLTEDEVRMLRGLISHVLNPRR
WRESNCRKG
>Mature_248_residues
SLERTRIVLVGTTHPGNIGAAARAMRTMGLRDLYLVDPQCDPGDPEAVARAAGAGDVLERARTAPDLATALTGCRLAFGL
SARSRAERQPRLELRPAAESAVAQGGDGDLAWVFGRERTGLTNAELDHCHYLVQIPTDPDYSSLNVAQSVQLVAWELRMA
AAAGGPVQGQAEEPPAAVDTLERFFDHLEEMAGRVGYLDRHNPALTMRRLRNIFRRAWLTEDEVRMLRGLISHVLNPRRW
RESNCRKG

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=236, Percent_Identity=45.7627118644068, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1790865, Length=227, Percent_Identity=33.4801762114537, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27413; Mature: 27282

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLERTRIVLVGTTHPGNIGAAARAMRTMGLRDLYLVDPQCDPGDPEAVARAAGAGDVLE
CCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCHHHHH
RARTAPDLATALTGCRLAFGLSARSRAERQPRLELRPAAESAVAQGGDGDLAWVFGRERT
HHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCHHHHHHCCCCCCEEEEECCCCC
GLTNAELDHCHYLVQIPTDPDYSSLNVAQSVQLVAWELRMAAAAGGPVQGQAEEPPAAVD
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
TLERFFDHLEEMAGRVGYLDRHNPALTMRRLRNIFRRAWLTEDEVRMLRGLISHVLNPRR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
WRESNCRKG
HCCCCCCCC
>Mature Secondary Structure 
SLERTRIVLVGTTHPGNIGAAARAMRTMGLRDLYLVDPQCDPGDPEAVARAAGAGDVLE
CCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCHHHHH
RARTAPDLATALTGCRLAFGLSARSRAERQPRLELRPAAESAVAQGGDGDLAWVFGRERT
HHHCCHHHHHHHHHHHHHHCCCHHHHHHCCCCCEECCCHHHHHHCCCCCCEEEEECCCCC
GLTNAELDHCHYLVQIPTDPDYSSLNVAQSVQLVAWELRMAAAAGGPVQGQAEEPPAAVD
CCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
TLERFFDHLEEMAGRVGYLDRHNPALTMRRLRNIFRRAWLTEDEVRMLRGLISHVLNPRR
HHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHH
WRESNCRKG
HCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14528314 [H]