Click here to switch to the map view.

The map label for this gene is truB

Identifier: 121998526

GI number: 121998526

Start: 1918348

End: 1919262

Strand: Reverse

Name: truB

Synonym: Hhal_1747

Alternate gene names: 121998526

Gene position: 1919262-1918348 (Counterclockwise)

Preceding gene: 121998527

Following gene: 121998525

Centisome position: 71.66

GC content: 68.42

Gene sequence:

>915_bases
ATGGCGAAGAGGCGGGGTGGGCGCAACGTCACCGGCGTGCTGTTGTTCGACAAGCCGGCTGGCGTGACCTCCAATAAGGC
CCTGCAACAGGTTAAGCGGCTCTTCGGGGCGCGCAAGGCTGGGCACACGGGCAGTCTCGATCCGCTGGCTACCGGCCTGC
TGCCGATCTGCTTCGGCGATGCAACCAAGGTATCCGGGTTCCTGCTCGATGCGGACAAACGGTACGTGGTGACCTGCAAG
CTCGGGGTGGCCACAGACACCGGCGACGCGGAGGGCACGGAACGCGATCGTGCCGAGGTCCCCGCGCTGGACGAGGAGGC
GGTCGAACGAGGTCTGACCGGGTTTCGCGGCCCCATCGACCAGCTGCCGCCCATGTACTCGGCCATCAAGCACCAGGGCC
AGCGACTCTACGACCTGGCGCGCCAGGGGGTGGAGGTCGAGCGCGAACCCCGCCGGGTTGAGATCTACGACCTGCAGCTG
GTGGCGGTTCGTGGGGATGAGCTGGAGCTGTCGGTGCACTGCTCCAAAGGCACCTACATCCGTACACTGGCCGAGGACAT
CGCCCAGGCGCTGGGGACCGTGGGACACGTGTGCGCGCTGCGCAGGCTCGGGCTGGGGCCGTACCAGGATCCGGCGATGT
GGACCGAAGAGATGCTCGCCGCGCGGGCGGAGCAGGGGCGCGAGGCGCTGGATGCCACACTGCTGCCGATGGACTCGGCC
CTCCAGCAGTACACCGGCGTCGAGCTTGCCGACGATCTCGCCTACTTTGTCGTCCAGGGGCAGCCGGTCTTTGTGCCCAA
GGCGCCCAGCGAGGGTTGGCTGCGGCTCTACGATCGCGGCGGCAACTTCCTGGGCATGGGGCAGGCCCTGGATGACGGGC
GCATCGGGCCCAAGCGCCTGGTGGCGCGCCGCTGA

Upstream 100 bases:

>100_bases
CACGACACCGCCTTCGATCGCGGTGCGCGGCTGAGTGCCCTGATCGACGAGGTGGCGCCGCCGCCGGCAGACGACGAACC
GAGCAAGGAGTAGAAGGCGA

Downstream 100 bases:

>100_bases
ACGGGGGCGGTGCTGCGCAAGCCCCCGCCGCCGGGTGGCAAATTCTTGTTCCCTTGCCCCCGCGAGGGCTACACTAGCTG
CCTTTATTGACAGGCCGAAA

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MAKRRGGRNVTGVLLFDKPAGVTSNKALQQVKRLFGARKAGHTGSLDPLATGLLPICFGDATKVSGFLLDADKRYVVTCK
LGVATDTGDAEGTERDRAEVPALDEEAVERGLTGFRGPIDQLPPMYSAIKHQGQRLYDLARQGVEVEREPRRVEIYDLQL
VAVRGDELELSVHCSKGTYIRTLAEDIAQALGTVGHVCALRRLGLGPYQDPAMWTEEMLAARAEQGREALDATLLPMDSA
LQQYTGVELADDLAYFVVQGQPVFVPKAPSEGWLRLYDRGGNFLGMGQALDDGRIGPKRLVARR

Sequences:

>Translated_304_residues
MAKRRGGRNVTGVLLFDKPAGVTSNKALQQVKRLFGARKAGHTGSLDPLATGLLPICFGDATKVSGFLLDADKRYVVTCK
LGVATDTGDAEGTERDRAEVPALDEEAVERGLTGFRGPIDQLPPMYSAIKHQGQRLYDLARQGVEVEREPRRVEIYDLQL
VAVRGDELELSVHCSKGTYIRTLAEDIAQALGTVGHVCALRRLGLGPYQDPAMWTEEMLAARAEQGREALDATLLPMDSA
LQQYTGVELADDLAYFVVQGQPVFVPKAPSEGWLRLYDRGGNFLGMGQALDDGRIGPKRLVARR
>Mature_303_residues
AKRRGGRNVTGVLLFDKPAGVTSNKALQQVKRLFGARKAGHTGSLDPLATGLLPICFGDATKVSGFLLDADKRYVVTCKL
GVATDTGDAEGTERDRAEVPALDEEAVERGLTGFRGPIDQLPPMYSAIKHQGQRLYDLARQGVEVEREPRRVEIYDLQLV
AVRGDELELSVHCSKGTYIRTLAEDIAQALGTVGHVCALRRLGLGPYQDPAMWTEEMLAARAEQGREALDATLLPMDSAL
QQYTGVELADDLAYFVVQGQPVFVPKAPSEGWLRLYDRGGNFLGMGQALDDGRIGPKRLVARR

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=258, Percent_Identity=32.9457364341085, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI215599015, Length=262, Percent_Identity=25.1908396946565, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4503337, Length=262, Percent_Identity=25.1908396946565, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI2367200, Length=307, Percent_Identity=48.5342019543974, Blast_Score=284, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI17553978, Length=262, Percent_Identity=23.6641221374046, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324037, Length=193, Percent_Identity=30.5699481865285, Blast_Score=85, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=265, Percent_Identity=26.4150943396226, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281364189, Length=266, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364187, Length=266, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364185, Length=266, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI281364183, Length=266, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI62471759, Length=266, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17975520, Length=266, Percent_Identity=26.3157894736842, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_HALHL (A1WXU9)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003313.1
- ProteinModelPortal:   A1WXU9
- SMR:   A1WXU9
- STRING:   A1WXU9
- GeneID:   4710480
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1747
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LNKVEKC
- PhylomeDB:   A1WXU9
- ProtClustDB:   PRK05033
- BioCyc:   HHAL349124:HHAL_1747-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 33133; Mature: 33002

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: ACT_SITE 47-47

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKRRGGRNVTGVLLFDKPAGVTSNKALQQVKRLFGARKAGHTGSLDPLATGLLPICFGD
CCCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHCCCC
ATKVSGFLLDADKRYVVTCKLGVATDTGDAEGTERDRAEVPALDEEAVERGLTGFRGPID
CHHHCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHH
QLPPMYSAIKHQGQRLYDLARQGVEVEREPRRVEIYDLQLVAVRGDELELSVHCSKGTYI
HCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCEEEEEEEECCCCHH
RTLAEDIAQALGTVGHVCALRRLGLGPYQDPAMWTEEMLAARAEQGREALDATLLPMDSA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LQQYTGVELADDLAYFVVQGQPVFVPKAPSEGWLRLYDRGGNFLGMGQALDDGRIGPKRL
HHHHCCCCHHHHHEEEEECCCEEEEECCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHH
VARR
HCCC
>Mature Secondary Structure 
AKRRGGRNVTGVLLFDKPAGVTSNKALQQVKRLFGARKAGHTGSLDPLATGLLPICFGD
CCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHCCCC
ATKVSGFLLDADKRYVVTCKLGVATDTGDAEGTERDRAEVPALDEEAVERGLTGFRGPID
CHHHCEEEEECCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCHH
QLPPMYSAIKHQGQRLYDLARQGVEVEREPRRVEIYDLQLVAVRGDELELSVHCSKGTYI
HCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCEEEEEEEECCCCHH
RTLAEDIAQALGTVGHVCALRRLGLGPYQDPAMWTEEMLAARAEQGREALDATLLPMDSA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
LQQYTGVELADDLAYFVVQGQPVFVPKAPSEGWLRLYDRGGNFLGMGQALDDGRIGPKRL
HHHHCCCCHHHHHEEEEECCCEEEEECCCCCCCEEEEECCCCEEECCCCCCCCCCCHHHH
VARR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA