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The map label for this gene is pnp

Identifier: 121998524

GI number: 121998524

Start: 1915824

End: 1917932

Strand: Reverse

Name: pnp

Synonym: Hhal_1745

Alternate gene names: 121998524

Gene position: 1917932-1915824 (Counterclockwise)

Preceding gene: 121998525

Following gene: 121998523

Centisome position: 71.61

GC content: 65.62

Gene sequence:

>2109_bases
ATGAGCGAGAGCCAGCTATCCCTGACCCCGGTCAAGCGGAGTTTTCAGTTCGGTCAGCACCAGGTGACCCTGGAGACCGG
GGGCGTGGCCCGTCAGGCCGATGGCGCCGTCCTGGTCGATATGGCGGACACGGTGGTTCTGGTCACCGTGGTCGGCAGGA
AGGACCCGGCGGCGGCACGCGATTTCCTGCCGCTGACGGTCAATTATCAGGAGCGCACCTACGCTGCGGGGCGTATCCCC
GGTGGCTTCTTCAAGCGGGAGGGCCGGCCCTCCGAGAAGGAGACCCTGACCTGCCGCCTGATCGACCGGCCGATCCGGCC
CCTGTTCCCCGAGGGGTTCCGCCAGGAGGTCCAGGTGATCGCCACGGTGCTCTCGCTCAACGATGAGGTGGATGCCGACA
TTCCGGCGATGATCGGGACCTCGGCGGCGCTGGCGTTGTCCGGCATTCCGTTCGAGGGGCCCATCGGTGCCTGCCGGGTT
GGTCACCTCGACGGCGAGTTCGTACTCAACCCGACCTTCAGTCAGACGGCGGAGTCGGATCTGGATCTGGTCGTGGCCGG
CACCCAGGATGCGGTCCTGATGGTCGAGTCCGAGGCCAATCTGCTGCCGGAGAGCACGATGCTCGACGCCGTGCTCTACG
GGCACGAGCAGATGCAGGTGGCCATCGATACCATCAACGATCTGGCCGCTGAGGCGGGCAAGCCGCGTTGGGACTGGCAG
CCGCCGGCGAAGAACGAGTCGCTGGACTCGGCGGTGGCAGAGCTGGTGCGTTCGGACCTCGAGGCCGCTTACCAGATCGC
CGACAAGCAGGAGCGCAGTGAGCGGGTTGGCGCCCTGCGCGACAAGGCGGTGGAGGCGCTGGCCGACGAGGAGCGTGAAG
GCTGGGGCGCCGGCGATGTCGGAGAGGCGTTCAAGACGCTCGAGAAGAAGATCGTCCGGGGCCGGATTCTGGCCGGTCAT
CCCCGCATCGATGGCCGCGACAACCAGACGGTGCGCCCGCTCAACGTGCAGGCCGGTGTCCTGCCGCGGACCCACGGCTC
GGCGATCTTCACCCGCGGCGAGACCCAGGCGATCGTGGTCAGCACGCTCGGTACAGGCCGGGATGCTCAGGTCATCGATG
CGCTGGAGGGCGAGCGGCGCGAGCCGTTTATGCTGCACTACAACTTCCCGCCGTACTGCGTGGGCGAGACTGGCTTCATC
GGCACGCCGAAGCGGCGCGAAATCGGCCACGGCAAGCTGGCCAAGCGCGGTGTTGAAGCGGTGATGCCCTCCGACGAGGA
GTTCCCCTACGTGATTCGCGTGGTCTCGGAGGTCACGGAGTCCAACGGGTCGTCTTCGATGGCCACCGTCTGTGGCACCA
GTCTGTCGCTGATGGATGCCGGCGTGCCGCTGAAGGCTCAGGTGGCTGGTATCGCCATGGGCCTGATCAAGGAAGGCGAC
GAGTTCGCCGTGCTCACCGACATCCTTGGCGACGAGGACCACCTCGGTGACATGGACTTCAAGGTTGCCGGTAGCAAGGA
TGGTGTCACTGCGCTGCAGATGGACATCAAGATCGACGGGATCACCCGGGAGATCATGGAACAGGCCCTGGATCAGGCGC
GCTCCGGCCGGCTGCATATCCTCGAGCAGATGAATCAGGTGATCGACAAGCCGCGCGAGCAGATGTCCGAGTACGCCCCG
CGGCTGCTGACCATGAGGATTGACCCGGAGCGTATCCGGGACGTGATCGGCAAGGGCGGTGCGACCATCCGTGGGCTGAC
CGAGGAGACCGGTACCAACATCGACATCAGCGACGAGGGCGTGGTGACCATCGCCTCGGCGGACAAGGCGGCCGCCGAAG
AGGCGAAGAAGCGCATCGAGCTGCTGACCGCCGATGTCGAGGTGGGCAAGGTCTACGATGGCAAGGTTGCGAAGCTCATG
GACTTCGGCGCCTTCGTCAACATCCTGCCCGGTCGGGATGGCTTGGTGCATATCTCGCAGATCTCCAACAACCGGGTCGA
GAACGTCGCCGACGAGCTTTCGGAGGGCGACTCGGTCCGGGTCAAGGTGCTCGAGGTCGATCGTCAGGGCCGGATCCGGC
TGTCGATGAAGGCCGTCGAAGAGGAGTAA

Upstream 100 bases:

>100_bases
CGCAAGCTGCTCGATTACCTCAAGCGCAAGGATCGGGGCCGTTATCAGGACGTCATCGAGCGTCTCGGCATTCGCAAGTA
AGCGCAAGGGGTAAACAACG

Downstream 100 bases:

>100_bases
CGGCCGCCGGACCACTGCAATCGCTGCGGTGTCGGCTTGCTGCCGGCGCCGCGGCGGCTACCCTGAAGGGGGTGCCGACC
GATCGTCGGGAGGTTGCGGT

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 702; Mature: 701

Protein sequence:

>702_residues
MSESQLSLTPVKRSFQFGQHQVTLETGGVARQADGAVLVDMADTVVLVTVVGRKDPAAARDFLPLTVNYQERTYAAGRIP
GGFFKREGRPSEKETLTCRLIDRPIRPLFPEGFRQEVQVIATVLSLNDEVDADIPAMIGTSAALALSGIPFEGPIGACRV
GHLDGEFVLNPTFSQTAESDLDLVVAGTQDAVLMVESEANLLPESTMLDAVLYGHEQMQVAIDTINDLAAEAGKPRWDWQ
PPAKNESLDSAVAELVRSDLEAAYQIADKQERSERVGALRDKAVEALADEEREGWGAGDVGEAFKTLEKKIVRGRILAGH
PRIDGRDNQTVRPLNVQAGVLPRTHGSAIFTRGETQAIVVSTLGTGRDAQVIDALEGERREPFMLHYNFPPYCVGETGFI
GTPKRREIGHGKLAKRGVEAVMPSDEEFPYVIRVVSEVTESNGSSSMATVCGTSLSLMDAGVPLKAQVAGIAMGLIKEGD
EFAVLTDILGDEDHLGDMDFKVAGSKDGVTALQMDIKIDGITREIMEQALDQARSGRLHILEQMNQVIDKPREQMSEYAP
RLLTMRIDPERIRDVIGKGGATIRGLTEETGTNIDISDEGVVTIASADKAAAEEAKKRIELLTADVEVGKVYDGKVAKLM
DFGAFVNILPGRDGLVHISQISNNRVENVADELSEGDSVRVKVLEVDRQGRIRLSMKAVEEE

Sequences:

>Translated_702_residues
MSESQLSLTPVKRSFQFGQHQVTLETGGVARQADGAVLVDMADTVVLVTVVGRKDPAAARDFLPLTVNYQERTYAAGRIP
GGFFKREGRPSEKETLTCRLIDRPIRPLFPEGFRQEVQVIATVLSLNDEVDADIPAMIGTSAALALSGIPFEGPIGACRV
GHLDGEFVLNPTFSQTAESDLDLVVAGTQDAVLMVESEANLLPESTMLDAVLYGHEQMQVAIDTINDLAAEAGKPRWDWQ
PPAKNESLDSAVAELVRSDLEAAYQIADKQERSERVGALRDKAVEALADEEREGWGAGDVGEAFKTLEKKIVRGRILAGH
PRIDGRDNQTVRPLNVQAGVLPRTHGSAIFTRGETQAIVVSTLGTGRDAQVIDALEGERREPFMLHYNFPPYCVGETGFI
GTPKRREIGHGKLAKRGVEAVMPSDEEFPYVIRVVSEVTESNGSSSMATVCGTSLSLMDAGVPLKAQVAGIAMGLIKEGD
EFAVLTDILGDEDHLGDMDFKVAGSKDGVTALQMDIKIDGITREIMEQALDQARSGRLHILEQMNQVIDKPREQMSEYAP
RLLTMRIDPERIRDVIGKGGATIRGLTEETGTNIDISDEGVVTIASADKAAAEEAKKRIELLTADVEVGKVYDGKVAKLM
DFGAFVNILPGRDGLVHISQISNNRVENVADELSEGDSVRVKVLEVDRQGRIRLSMKAVEEE
>Mature_701_residues
SESQLSLTPVKRSFQFGQHQVTLETGGVARQADGAVLVDMADTVVLVTVVGRKDPAAARDFLPLTVNYQERTYAAGRIPG
GFFKREGRPSEKETLTCRLIDRPIRPLFPEGFRQEVQVIATVLSLNDEVDADIPAMIGTSAALALSGIPFEGPIGACRVG
HLDGEFVLNPTFSQTAESDLDLVVAGTQDAVLMVESEANLLPESTMLDAVLYGHEQMQVAIDTINDLAAEAGKPRWDWQP
PAKNESLDSAVAELVRSDLEAAYQIADKQERSERVGALRDKAVEALADEEREGWGAGDVGEAFKTLEKKIVRGRILAGHP
RIDGRDNQTVRPLNVQAGVLPRTHGSAIFTRGETQAIVVSTLGTGRDAQVIDALEGERREPFMLHYNFPPYCVGETGFIG
TPKRREIGHGKLAKRGVEAVMPSDEEFPYVIRVVSEVTESNGSSSMATVCGTSLSLMDAGVPLKAQVAGIAMGLIKEGDE
FAVLTDILGDEDHLGDMDFKVAGSKDGVTALQMDIKIDGITREIMEQALDQARSGRLHILEQMNQVIDKPREQMSEYAPR
LLTMRIDPERIRDVIGKGGATIRGLTEETGTNIDISDEGVVTIASADKAAAEEAKKRIELLTADVEVGKVYDGKVAKLMD
FGAFVNILPGRDGLVHISQISNNRVENVADELSEGDSVRVKVLEVDRQGRIRLSMKAVEEE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=713, Percent_Identity=37.3071528751753, Blast_Score=441, Evalue=1e-124,
Organism=Escherichia coli, GI145693187, Length=695, Percent_Identity=65.1798561151079, Blast_Score=931, Evalue=0.0,
Organism=Escherichia coli, GI87082262, Length=78, Percent_Identity=47.4358974358974, Blast_Score=65, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=34.1292134831461, Blast_Score=356, Evalue=3e-98,
Organism=Caenorhabditis elegans, GI17535281, Length=78, Percent_Identity=50, Blast_Score=76, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6320850, Length=103, Percent_Identity=36.8932038834951, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281362905, Length=719, Percent_Identity=37.413073713491, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651641, Length=719, Percent_Identity=37.413073713491, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24651643, Length=719, Percent_Identity=37.413073713491, Blast_Score=444, Evalue=1e-124,
Organism=Drosophila melanogaster, GI161079377, Length=662, Percent_Identity=37.4622356495468, Blast_Score=406, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_HALHL (A1WXU7)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003311.1
- ProteinModelPortal:   A1WXU7
- SMR:   A1WXU7
- STRING:   A1WXU7
- GeneID:   4710476
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1745
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   A1WXU7
- ProtClustDB:   PRK11824
- BioCyc:   HHAL349124:HHAL_1745-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76174; Mature: 76043

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESQLSLTPVKRSFQFGQHQVTLETGGVARQADGAVLVDMADTVVLVTVVGRKDPAAAR
CCCCCEEECHHHHHHHCCCEEEEEECCCCEECCCCEEEEEECCEEEEEEEECCCCCCCHH
DFLPLTVNYQERTYAAGRIPGGFFKREGRPSEKETLTCRLIDRPIRPLFPEGFRQEVQVI
CCEEEEEECCHHEEECCCCCCHHHCCCCCCCHHHEEEEEEECCCCCCCCCHHHHHHHHHH
ATVLSLNDEVDADIPAMIGTSAALALSGIPFEGPIGACRVGHLDGEFVLNPTFSQTAESD
HHHHHCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCEEEECCCCCEEECCCHHHCCCCC
LDLVVAGTQDAVLMVESEANLLPESTMLDAVLYGHEQMQVAIDTINDLAAEAGKPRWDWQ
CEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHCCHHEEEHHHHHHHHHHHCCCCCCCCC
PPAKNESLDSAVAELVRSDLEAAYQIADKQERSERVGALRDKAVEALADEEREGWGAGDV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
GEAFKTLEKKIVRGRILAGHPRIDGRDNQTVRPLNVQAGVLPRTHGSAIFTRGETQAIVV
HHHHHHHHHHHHHCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEE
STLGTGRDAQVIDALEGERREPFMLHYNFPPYCVGETGFIGTPKRREIGHGKLAKRGVEA
EECCCCCCCHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHCHHH
VMPSDEEFPYVIRVVSEVTESNGSSSMATVCGTSLSLMDAGVPLKAQVAGIAMGLIKEGD
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCC
EFAVLTDILGDEDHLGDMDFKVAGSKDGVTALQMDIKIDGITREIMEQALDQARSGRLHI
CEEEEEHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHHHHCCCHHH
LEQMNQVIDKPREQMSEYAPRLLTMRIDPERIRDVIGKGGATIRGLTEETGTNIDISDEG
HHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHCCCCCEEEECHHCCCCEEEECCCC
VVTIASADKAAAEEAKKRIELLTADVEVGKVYDGKVAKLMDFGAFVNILPGRDGLVHISQ
EEEEECCCHHHHHHHHHHHHHEEECCEECCCCCCHHHHHHHHCCCEEECCCCCCEEEEEE
ISNNRVENVADELSEGDSVRVKVLEVDRQGRIRLSMKAVEEE
CCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCC
>Mature Secondary Structure 
SESQLSLTPVKRSFQFGQHQVTLETGGVARQADGAVLVDMADTVVLVTVVGRKDPAAAR
CCCCEEECHHHHHHHCCCEEEEEECCCCEECCCCEEEEEECCEEEEEEEECCCCCCCHH
DFLPLTVNYQERTYAAGRIPGGFFKREGRPSEKETLTCRLIDRPIRPLFPEGFRQEVQVI
CCEEEEEECCHHEEECCCCCCHHHCCCCCCCHHHEEEEEEECCCCCCCCCHHHHHHHHHH
ATVLSLNDEVDADIPAMIGTSAALALSGIPFEGPIGACRVGHLDGEFVLNPTFSQTAESD
HHHHHCCCCCCCCCCHHHCCCHHHHCCCCCCCCCCCCEEEECCCCCEEECCCHHHCCCCC
LDLVVAGTQDAVLMVESEANLLPESTMLDAVLYGHEQMQVAIDTINDLAAEAGKPRWDWQ
CEEEEECCCCEEEEEECCCCCCCCHHHHHHHHHCCHHEEEHHHHHHHHHHHCCCCCCCCC
PPAKNESLDSAVAELVRSDLEAAYQIADKQERSERVGALRDKAVEALADEEREGWGAGDV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
GEAFKTLEKKIVRGRILAGHPRIDGRDNQTVRPLNVQAGVLPRTHGSAIFTRGETQAIVV
HHHHHHHHHHHHHCCEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCEEEECCCCCEEEE
STLGTGRDAQVIDALEGERREPFMLHYNFPPYCVGETGFIGTPKRREIGHGKLAKRGVEA
EECCCCCCCHHHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCHHHHHCHHH
VMPSDEEFPYVIRVVSEVTESNGSSSMATVCGTSLSLMDAGVPLKAQVAGIAMGLIKEGD
CCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCC
EFAVLTDILGDEDHLGDMDFKVAGSKDGVTALQMDIKIDGITREIMEQALDQARSGRLHI
CEEEEEHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEECHHHHHHHHHHHHHHHCCCHHH
LEQMNQVIDKPREQMSEYAPRLLTMRIDPERIRDVIGKGGATIRGLTEETGTNIDISDEG
HHHHHHHHHHHHHHHHHHCCEEEEEEECHHHHHHHHCCCCCEEEECHHCCCCEEEECCCC
VVTIASADKAAAEEAKKRIELLTADVEVGKVYDGKVAKLMDFGAFVNILPGRDGLVHISQ
EEEEECCCHHHHHHHHHHHHHEEECCEECCCCCCHHHHHHHHCCCEEECCCCCCEEEEEE
ISNNRVENVADELSEGDSVRVKVLEVDRQGRIRLSMKAVEEE
CCCCHHHHHHHHHCCCCCEEEEEEEECCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA