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The map label for this gene is dnaE [H]

Identifier: 121998458

GI number: 121998458

Start: 1831992

End: 1833764

Strand: Reverse

Name: dnaE [H]

Synonym: Hhal_1679

Alternate gene names: 121998458

Gene position: 1833764-1831992 (Counterclockwise)

Preceding gene: 121998459

Following gene: 121998457

Centisome position: 68.46

GC content: 69.99

Gene sequence:

>1773_bases
ATGCACGACGACGCCACGACCATCGTTCCGTTCCGTGGGGGCGCGCTGGAGCGCGCCGCCTGGGATCGGCTTTGGGATGC
CGCCGATGGTGGCTGCGTGCGCCGGGCCCTCGGCGAGCGGCGTACGCGCTACCAGCGGCGCGTCGAGCAGGAGATGGCAG
CCTTTGCACGCCTGGGTCTGGAGCCGGGGGTGCTGCTGGAACTCTCCTGGGTGGTGCAGGATCTGCGGGGCCGTGGAGTC
ACCTTCGGGCCGGGGTACGGCCCGCAGAGCGGGTCGCTGCTGCTGCACCTGCTGGGTCTCAACGGTATCGATCCCATCGA
TCACGGGTTGCCGTTCCTCGGCTTCGGGCGCGAGCTGGAGGCCCGGCGTCTGCACTTGCGCGTCGCCACCCGCGCCGAGC
AGGCGGAACTGGGCTCGGCGTTGCGCCAGGGGGGTGCGGCCGCTGCGGCGCTGCCGGAACAGGGGATCGAGGTCGTGGTG
GGGCGCGACCCGACGCTCGCCTTGACCTGCGAGATGGTCCGCCTGGCCCGTGCACTCAACGACAACCGCGACTGCGATCC
CCTGCGTTGGGCCTATCCGTGGGATGATCCGGATACCCTGCGCCTGATCGCCCGCGGGGAGACCGCCGGGATCCCGCTGC
TGGAGGATGCGCAGTTGCAGGAGCGCATGCGGGAGGCGGCGCCGCGCAGTCTCGAGGAAGTGACACAGGTCGTGGTGGCG
GCCCGAGATGGGGCGCCGCACGGTCTGCTCGAGCGGTATCGGGCGGCGCGCACGGGCGCGAGCCGTCCGCCATTGCCCCA
TCCCGAGCTCGAGGACATCCTCGCCCCCACGGGATACATCTGGCTCTACCGCGAGCAGGTGGTCGCGGCACTGGCCCGGT
TGCTCGGTTGTGAGCTGGCCGAGGCGGTCGGACTCGCACAACGGCTAAGCGAGGAGGGGGGCGATGCCCACGAGGGGGAG
CTTTCCCGGCAGCTCGGCGAGCGCATCGCTGCCCGTTGCCAGCTTCCCTACGCGCGGGGGCTTTATCTGCTTCGTGCCCT
GCGCCAGGGCCTGGCCCAGGTGGTGGATCGGGCGGCCACGCTGGTCGAGGTCACCGAGGCTTACCGTCAGGCCTACTACA
AGGCACGCTACCCGGCGGTTTTCCAGGCTGCGCGGCTGAACCGGGCGCTGCGGCGGGTGACGCCTCGGGGCGGTGTGCGT
TATGGGGTGGGAGGCGGGCCGGATGGCGTCTGTCCGCATCGGGAGATTCTCATTCGCCTCTGCAAGGAGGCGTTGAGTGA
CGGTGTGACCGTGCTGCCACCGGATATCAATCGCAGCGACTGGGGGTTTCGCCCCCTGGATCGGAACACGGTGATCTACG
GCCTCGGGGCGATTGCGCAGATCGACCGGCAGACCGCCGACCGACTGGTGCTGCGCCGCCGCGGGGGTGAGTACCGCAGC
CTGATGGATCTCATCATCCGGGGGTTCGGAGGCACCGCGCCGGCGATACAGGTTGAGCCGCTGATCTTCTCCGGTGCGCT
GGACGAGTTCGGCCGCGATCGCTGGCACTTGCGCAGGGAGCTCGATGAGGCGCAGGCCGTCCGAGGGTGGCAATACGCCG
CGGATGGGCGGACAACCCACTCTCTGGCGGGCGGTTTGGATGCCTGGCAGGCCGTTCATCCCGGGGAGGGATGGGCCTTC
CAGCGGGAACTTGATGTGCTCGCCTGTGTCCCGTCACGTCAGTACTTTCCGGAACCTGCCCCGGCCGAACGGCGCTTGCG
CCCGGTCCGGTGA

Upstream 100 bases:

>100_bases
CCGGCGAGGCGGCCGCCGGGCTGCTGCGTGAATTCTTTCGCGCCCGGCGTTAACCCCGGCCGCGTCGACGGTCTGGCTCC
GGCGCGGCTATAGTCAGAAC

Downstream 100 bases:

>100_bases
CCCTGTGGGGCCGGGGCTTGAATTCGCCGCCGATCTCCCGCACAGTATGTCCACGCTGACGGCCGACGCGGTCGTATAGG
CATGTAAAGGAAGGTGCCGA

Product: DNA polymerase III subunit alpha-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 590; Mature: 590

Protein sequence:

>590_residues
MHDDATTIVPFRGGALERAAWDRLWDAADGGCVRRALGERRTRYQRRVEQEMAAFARLGLEPGVLLELSWVVQDLRGRGV
TFGPGYGPQSGSLLLHLLGLNGIDPIDHGLPFLGFGRELEARRLHLRVATRAEQAELGSALRQGGAAAAALPEQGIEVVV
GRDPTLALTCEMVRLARALNDNRDCDPLRWAYPWDDPDTLRLIARGETAGIPLLEDAQLQERMREAAPRSLEEVTQVVVA
ARDGAPHGLLERYRAARTGASRPPLPHPELEDILAPTGYIWLYREQVVAALARLLGCELAEAVGLAQRLSEEGGDAHEGE
LSRQLGERIAARCQLPYARGLYLLRALRQGLAQVVDRAATLVEVTEAYRQAYYKARYPAVFQAARLNRALRRVTPRGGVR
YGVGGGPDGVCPHREILIRLCKEALSDGVTVLPPDINRSDWGFRPLDRNTVIYGLGAIAQIDRQTADRLVLRRRGGEYRS
LMDLIIRGFGGTAPAIQVEPLIFSGALDEFGRDRWHLRRELDEAQAVRGWQYAADGRTTHSLAGGLDAWQAVHPGEGWAF
QRELDVLACVPSRQYFPEPAPAERRLRPVR

Sequences:

>Translated_590_residues
MHDDATTIVPFRGGALERAAWDRLWDAADGGCVRRALGERRTRYQRRVEQEMAAFARLGLEPGVLLELSWVVQDLRGRGV
TFGPGYGPQSGSLLLHLLGLNGIDPIDHGLPFLGFGRELEARRLHLRVATRAEQAELGSALRQGGAAAAALPEQGIEVVV
GRDPTLALTCEMVRLARALNDNRDCDPLRWAYPWDDPDTLRLIARGETAGIPLLEDAQLQERMREAAPRSLEEVTQVVVA
ARDGAPHGLLERYRAARTGASRPPLPHPELEDILAPTGYIWLYREQVVAALARLLGCELAEAVGLAQRLSEEGGDAHEGE
LSRQLGERIAARCQLPYARGLYLLRALRQGLAQVVDRAATLVEVTEAYRQAYYKARYPAVFQAARLNRALRRVTPRGGVR
YGVGGGPDGVCPHREILIRLCKEALSDGVTVLPPDINRSDWGFRPLDRNTVIYGLGAIAQIDRQTADRLVLRRRGGEYRS
LMDLIIRGFGGTAPAIQVEPLIFSGALDEFGRDRWHLRRELDEAQAVRGWQYAADGRTTHSLAGGLDAWQAVHPGEGWAF
QRELDVLACVPSRQYFPEPAPAERRLRPVR
>Mature_590_residues
MHDDATTIVPFRGGALERAAWDRLWDAADGGCVRRALGERRTRYQRRVEQEMAAFARLGLEPGVLLELSWVVQDLRGRGV
TFGPGYGPQSGSLLLHLLGLNGIDPIDHGLPFLGFGRELEARRLHLRVATRAEQAELGSALRQGGAAAAALPEQGIEVVV
GRDPTLALTCEMVRLARALNDNRDCDPLRWAYPWDDPDTLRLIARGETAGIPLLEDAQLQERMREAAPRSLEEVTQVVVA
ARDGAPHGLLERYRAARTGASRPPLPHPELEDILAPTGYIWLYREQVVAALARLLGCELAEAVGLAQRLSEEGGDAHEGE
LSRQLGERIAARCQLPYARGLYLLRALRQGLAQVVDRAATLVEVTEAYRQAYYKARYPAVFQAARLNRALRRVTPRGGVR
YGVGGGPDGVCPHREILIRLCKEALSDGVTVLPPDINRSDWGFRPLDRNTVIYGLGAIAQIDRQTADRLVLRRRGGEYRS
LMDLIIRGFGGTAPAIQVEPLIFSGALDEFGRDRWHLRRELDEAQAVRGWQYAADGRTTHSLAGGLDAWQAVHPGEGWAF
QRELDVLACVPSRQYFPEPAPAERRLRPVR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The alpha chain is the DNA polymerase [H]

COG id: COG0587

COG function: function code L; DNA polymerase III, alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-C family. DnaE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786381, Length=363, Percent_Identity=24.7933884297521, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011708
- InterPro:   IPR004365
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR004805 [H]

Pfam domain/function: PF07733 DNA_pol3_alpha; PF02811 PHP; PF01336 tRNA_anti [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 65274; Mature: 65274

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDDATTIVPFRGGALERAAWDRLWDAADGGCVRRALGERRTRYQRRVEQEMAAFARLGL
CCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EPGVLLELSWVVQDLRGRGVTFGPGYGPQSGSLLLHLLGLNGIDPIDHGLPFLGFGRELE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEECCCCCCHHHHCCCCCCCCCCHH
ARRLHLRVATRAEQAELGSALRQGGAAAAALPEQGIEVVVGRDPTLALTCEMVRLARALN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHC
DNRDCDPLRWAYPWDDPDTLRLIARGETAGIPLLEDAQLQERMREAAPRSLEEVTQVVVA
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
ARDGAPHGLLERYRAARTGASRPPLPHPELEDILAPTGYIWLYREQVVAALARLLGCELA
HCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
EAVGLAQRLSEEGGDAHEGELSRQLGERIAARCQLPYARGLYLLRALRQGLAQVVDRAAT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVEVTEAYRQAYYKARYPAVFQAARLNRALRRVTPRGGVRYGVGGGPDGVCPHREILIRL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHH
CKEALSDGVTVLPPDINRSDWGFRPLDRNTVIYGLGAIAQIDRQTADRLVLRRRGGEYRS
HHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHH
LMDLIIRGFGGTAPAIQVEPLIFSGALDEFGRDRWHLRRELDEAQAVRGWQYAADGRTTH
HHHHHHHHCCCCCCCEEECCCHHHCCHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCHH
SLAGGLDAWQAVHPGEGWAFQRELDVLACVPSRQYFPEPAPAERRLRPVR
HHHCCHHHHHCCCCCCCCEEHHCCCCEEECCCCCCCCCCCCHHHCCCCCC
>Mature Secondary Structure
MHDDATTIVPFRGGALERAAWDRLWDAADGGCVRRALGERRTRYQRRVEQEMAAFARLGL
CCCCCCEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
EPGVLLELSWVVQDLRGRGVTFGPGYGPQSGSLLLHLLGLNGIDPIDHGLPFLGFGRELE
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHEECCCCCCHHHHCCCCCCCCCCHH
ARRLHLRVATRAEQAELGSALRQGGAAAAALPEQGIEVVVGRDPTLALTCEMVRLARALN
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHC
DNRDCDPLRWAYPWDDPDTLRLIARGETAGIPLLEDAQLQERMREAAPRSLEEVTQVVVA
CCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
ARDGAPHGLLERYRAARTGASRPPLPHPELEDILAPTGYIWLYREQVVAALARLLGCELA
HCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHH
EAVGLAQRLSEEGGDAHEGELSRQLGERIAARCQLPYARGLYLLRALRQGLAQVVDRAAT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LVEVTEAYRQAYYKARYPAVFQAARLNRALRRVTPRGGVRYGVGGGPDGVCPHREILIRL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHH
CKEALSDGVTVLPPDINRSDWGFRPLDRNTVIYGLGAIAQIDRQTADRLVLRRRGGEYRS
HHHHHHCCCEEECCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCHHHH
LMDLIIRGFGGTAPAIQVEPLIFSGALDEFGRDRWHLRRELDEAQAVRGWQYAADGRTTH
HHHHHHHHCCCCCCCEEECCCHHHCCHHHHCCHHHHHHHHHHHHHHHCCCEECCCCCCHH
SLAGGLDAWQAVHPGEGWAFQRELDVLACVPSRQYFPEPAPAERRLRPVR
HHHCCHHHHHCCCCCCCCEEHHCCCCEEECCCCCCCCCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]