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The map label for this gene is wcaJ [H]

Identifier: 121998317

GI number: 121998317

Start: 1669082

End: 1670491

Strand: Reverse

Name: wcaJ [H]

Synonym: Hhal_1535

Alternate gene names: 121998317

Gene position: 1670491-1669082 (Counterclockwise)

Preceding gene: 121998318

Following gene: 121998316

Centisome position: 62.37

GC content: 61.42

Gene sequence:

>1410_bases
GTGCGGTTTTTCGGTCATTATATCCCGCGCAAGGTTCTTTTACTCGCTGGATTTGAAGGGGTGCTGGTATTCCTCGGCTT
GACGGCTATCCTCCCGCTGCAGGCCGGCCTGGTGGGGCCGTATGGCCTCGAGCAGCTGCCATTGCAGCTGGAGATGGTGG
TTGTAGCCCTGGCCGTGCTTCTGAGCATGGCGAGTCTGGGTCTTTACGACACCGGGCAGATGGCGATGCGCGATGGGCAG
CTGATGTTAGGGGTCCGGCTGCTGTTGGCCATGGTTGCGACCGGGGTCGGCATGTGGGTCCTACTGGCACTGATGCCGGC
GCTGGCGATCGAGCCGAGCGTGCTGGTGCTTGGCCTGGTGGTGGCATTCTTCCTACTGCTAATGGGCCGATTGCTGTCCA
GCCGAGTTGTTACTGCGAATAAGCTCAAGCCCCGCGTCTTGGTGCTGGGTGTGGGCTCACGCGCGACGGCGGTGACCGAG
CTCCAGGGCGAGCGCGGCCGTGAGGAACTGCCGTACGTCATGGTCGGCTATGTGGCCCCGCCGGGAACCGACCATGTTGA
CGTCCCCGCCGATGAGGTCATCGAGCAAGAGCAAGGGACGTGTCTGCTTGAGCTGTGTCGGCGCCATGGGATCAACGAAC
TAGTTGTGGGGGTTCGCGACCGTCGCGGGCAGCTTCCGGTGCGCGATCTGCTGGAATGCAAACTCCACGAGATCTCGGTG
ATCGACCTCACCCGATTCCTCGAGCGCGAGCGTCGGCAGGTCCGGTTGGACTCCCTGAATACCAGTTCGTTGATCTTTGG
CGAGGGGTTCCGTCAGGACGGGGTGCGCGTCACCGTCAAACGAGCCTTTGATCTGATGGTTAGTCTCGCCCTTCTGTTGG
TTACCTTGCCGATCATGCTGATTACGGCACTCGCCATTAAGCTGGAGAGTCCGGGGTCGGTGTTCTTCGGCCAGGAGCGG
GTCGGCATCGGCAACAAGCCGTACCGGCTGTTTAAGTTCCGCAGTATGCGCGAGGACGCTGAGCAGGACGGTGTGCCGCA
GTGGGCGCAGCAGGGAGATGATCGCATCACACGGGTGGGTGCTGTGATCCGCAAACTTCGCATCGACGAGCTGCCGCAGA
TCCTGAACGTCCTCAAGGGCGAGATGTCTTTCGTCGGGCCGCGCCCCGAGCGGCCGCTGTTTGTTGAACGTCTAGGTGGG
GAGATTCCGCTTTACCGGTTTCGGCACACGGTGGCGCCGGGGATTACCGGTTGGGCGCAGGTCTGTTATCCCTACGGCGC
ATCGATCCAGGATGCACGAGAAAAGCTCCAGTACGATTTGTACTACCTGAAGAACCATACCCTGTTTCTGGATCTGGTTA
TCCTGATCCGCACCGTGGCCGTGGTGCTCTTCGGTCAGGGCGCCCGCTAG

Upstream 100 bases:

>100_bases
CAAGCCCGGCGACGTGCTGATTATACCGGAACGATTTTTCTGATATCGGCTCGGCCATGGATTGGGGGTGCAGGGAACTG
CGCCCCCTTGGAGGCGCGCA

Downstream 100 bases:

>100_bases
GCGCTGCGGAGGCGCGATGCTATCGGCTGCGAGTTTTGCCGCTGCTTTGGCGGCGTTCCTGGTGCTGGCTGTTCTGACAC
TCACACTGTGGCGGGATCGG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MRFFGHYIPRKVLLLAGFEGVLVFLGLTAILPLQAGLVGPYGLEQLPLQLEMVVVALAVLLSMASLGLYDTGQMAMRDGQ
LMLGVRLLLAMVATGVGMWVLLALMPALAIEPSVLVLGLVVAFFLLLMGRLLSSRVVTANKLKPRVLVLGVGSRATAVTE
LQGERGREELPYVMVGYVAPPGTDHVDVPADEVIEQEQGTCLLELCRRHGINELVVGVRDRRGQLPVRDLLECKLHEISV
IDLTRFLERERRQVRLDSLNTSSLIFGEGFRQDGVRVTVKRAFDLMVSLALLLVTLPIMLITALAIKLESPGSVFFGQER
VGIGNKPYRLFKFRSMREDAEQDGVPQWAQQGDDRITRVGAVIRKLRIDELPQILNVLKGEMSFVGPRPERPLFVERLGG
EIPLYRFRHTVAPGITGWAQVCYPYGASIQDAREKLQYDLYYLKNHTLFLDLVILIRTVAVVLFGQGAR

Sequences:

>Translated_469_residues
MRFFGHYIPRKVLLLAGFEGVLVFLGLTAILPLQAGLVGPYGLEQLPLQLEMVVVALAVLLSMASLGLYDTGQMAMRDGQ
LMLGVRLLLAMVATGVGMWVLLALMPALAIEPSVLVLGLVVAFFLLLMGRLLSSRVVTANKLKPRVLVLGVGSRATAVTE
LQGERGREELPYVMVGYVAPPGTDHVDVPADEVIEQEQGTCLLELCRRHGINELVVGVRDRRGQLPVRDLLECKLHEISV
IDLTRFLERERRQVRLDSLNTSSLIFGEGFRQDGVRVTVKRAFDLMVSLALLLVTLPIMLITALAIKLESPGSVFFGQER
VGIGNKPYRLFKFRSMREDAEQDGVPQWAQQGDDRITRVGAVIRKLRIDELPQILNVLKGEMSFVGPRPERPLFVERLGG
EIPLYRFRHTVAPGITGWAQVCYPYGASIQDAREKLQYDLYYLKNHTLFLDLVILIRTVAVVLFGQGAR
>Mature_469_residues
MRFFGHYIPRKVLLLAGFEGVLVFLGLTAILPLQAGLVGPYGLEQLPLQLEMVVVALAVLLSMASLGLYDTGQMAMRDGQ
LMLGVRLLLAMVATGVGMWVLLALMPALAIEPSVLVLGLVVAFFLLLMGRLLSSRVVTANKLKPRVLVLGVGSRATAVTE
LQGERGREELPYVMVGYVAPPGTDHVDVPADEVIEQEQGTCLLELCRRHGINELVVGVRDRRGQLPVRDLLECKLHEISV
IDLTRFLERERRQVRLDSLNTSSLIFGEGFRQDGVRVTVKRAFDLMVSLALLLVTLPIMLITALAIKLESPGSVFFGQER
VGIGNKPYRLFKFRSMREDAEQDGVPQWAQQGDDRITRVGAVIRKLRIDELPQILNVLKGEMSFVGPRPERPLFVERLGG
EIPLYRFRHTVAPGITGWAQVCYPYGASIQDAREKLQYDLYYLKNHTLFLDLVILIRTVAVVLFGQGAR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=189, Percent_Identity=41.2698412698413, Blast_Score=147, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 52116; Mature: 52116

Theoretical pI: Translated: 9.04; Mature: 9.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFFGHYIPRKVLLLAGFEGVLVFLGLTAILPLQAGLVGPYGLEQLPLQLEMVVVALAVL
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHH
LSMASLGLYDTGQMAMRDGQLMLGVRLLLAMVATGVGMWVLLALMPALAIEPSVLVLGLV
HHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VAFFLLLMGRLLSSRVVTANKLKPRVLVLGVGSRATAVTELQGERGREELPYVMVGYVAP
HHHHHHHHHHHHHHCCCHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCEEEEEEECC
PGTDHVDVPADEVIEQEQGTCLLELCRRHGINELVVGVRDRRGQLPVRDLLECKLHEISV
CCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
IDLTRFLERERRQVRLDSLNTSSLIFGEGFRQDGVRVTVKRAFDLMVSLALLLVTLPIML
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
ITALAIKLESPGSVFFGQERVGIGNKPYRLFKFRSMREDAEQDGVPQWAQQGDDRITRVG
HHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHH
AVIRKLRIDELPQILNVLKGEMSFVGPRPERPLFVERLGGEIPLYRFRHTVAPGITGWAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCHHHH
VCYPYGASIQDAREKLQYDLYYLKNHTLFLDLVILIRTVAVVLFGQGAR
HHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRFFGHYIPRKVLLLAGFEGVLVFLGLTAILPLQAGLVGPYGLEQLPLQLEMVVVALAVL
CCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHH
LSMASLGLYDTGQMAMRDGQLMLGVRLLLAMVATGVGMWVLLALMPALAIEPSVLVLGLV
HHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
VAFFLLLMGRLLSSRVVTANKLKPRVLVLGVGSRATAVTELQGERGREELPYVMVGYVAP
HHHHHHHHHHHHHHCCCHHCCCCCEEEEEECCCCHHHHHHHCCCCCCCCCCEEEEEEECC
PGTDHVDVPADEVIEQEQGTCLLELCRRHGINELVVGVRDRRGQLPVRDLLECKLHEISV
CCCCCCCCCHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
IDLTRFLERERRQVRLDSLNTSSLIFGEGFRQDGVRVTVKRAFDLMVSLALLLVTLPIML
HHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
ITALAIKLESPGSVFFGQERVGIGNKPYRLFKFRSMREDAEQDGVPQWAQQGDDRITRVG
HHHHHHHCCCCCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHH
AVIRKLRIDELPQILNVLKGEMSFVGPRPERPLFVERLGGEIPLYRFRHTVAPGITGWAQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCHHHH
VCYPYGASIQDAREKLQYDLYYLKNHTLFLDLVILIRTVAVVLFGQGAR
HHHCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]