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The map label for this gene is ispD

Identifier: 121998217

GI number: 121998217

Start: 1547011

End: 1547739

Strand: Reverse

Name: ispD

Synonym: Hhal_1435

Alternate gene names: 121998217

Gene position: 1547739-1547011 (Counterclockwise)

Preceding gene: 121998218

Following gene: 121998216

Centisome position: 57.78

GC content: 72.02

Gene sequence:

>729_bases
ATGGGGCGCTACTGGGCGGTGATACCGGCGGCCGGTGTGGGCCGGCGTATGGGGGGCGGGGATCGCCCCAAACAGTATCG
CCTGCTGCTGGGGCGGCCGGTGATCGGCTGGGCCCTGGAACGGCTGCTCGGTCACCCGAAGGTGGCCGGGGCCGTGGTGG
CGTTGGCCGCCGACGACCCCCACTGGGACGCCCTGGGACTGGACAGGCAGGTGGCGGGGAAACCCGTGCACCGCGTGGAA
GGGGGGGCAGAGCGGCGTGATTCGGTTCGGGCCGCGCTGGCCTACCTGGGCGGGATCGCCAACCCCGAGGATCGGGTGCT
GGTCCACGACGCCGTACGGCCGTGCCTGAGTGCGGCCGAACTGGACCGACTGATCGACGAGGGGGGGGCAGCCGTCGACG
GCGCCCTGCTGGCGACCCCGGTCCGCGATACCCTCAAGCGCGCCGATGGCGACTGCGTCGGTGCCACCGTCTCCCGGGAG
GGGCTGTGGCAGGCCCAGACGCCTCAGCTTTTCCCGCTGCGGCGTCTGTCGGCGGCGCTGGACGCGGCCTTGGCAGCTGG
TGTGGCCGTCACCGACGAGGCCCAGGCTGTGGAATGGCATGACGGCCAGCCGCGGCTGGTCACTGGCGAAGCCGGCAATC
TGAAGATCACCCATCAGGCTGACCTGGATCTGGCTGCTGCCGTGCTTACGGCGCAGCGGGCGGCCACGGAGAGGGAGCAA
ACCGCGTGA

Upstream 100 bases:

>100_bases
AGAATCTTAAGGAGAGCACCGAGGCCCTGGAGGAACGGGCCCGTTATGAACTCGGTATGATCCGGGACGACGAGGTCTTC
TACCAGGTTGTGGAGGAGTA

Downstream 100 bases:

>100_bases
TTCGCATTGGGCAGGGTTTCGACGTCCACCGGTTCGCCGGCAAGGGCGAGGGTTTTCGGCTGGGCGGGGTCGCGGTCCCG
TACCACAAGCGGTTGGAGGC

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MGRYWAVIPAAGVGRRMGGGDRPKQYRLLLGRPVIGWALERLLGHPKVAGAVVALAADDPHWDALGLDRQVAGKPVHRVE
GGAERRDSVRAALAYLGGIANPEDRVLVHDAVRPCLSAAELDRLIDEGGAAVDGALLATPVRDTLKRADGDCVGATVSRE
GLWQAQTPQLFPLRRLSAALDAALAAGVAVTDEAQAVEWHDGQPRLVTGEAGNLKITHQADLDLAAAVLTAQRAATEREQ
TA

Sequences:

>Translated_242_residues
MGRYWAVIPAAGVGRRMGGGDRPKQYRLLLGRPVIGWALERLLGHPKVAGAVVALAADDPHWDALGLDRQVAGKPVHRVE
GGAERRDSVRAALAYLGGIANPEDRVLVHDAVRPCLSAAELDRLIDEGGAAVDGALLATPVRDTLKRADGDCVGATVSRE
GLWQAQTPQLFPLRRLSAALDAALAAGVAVTDEAQAVEWHDGQPRLVTGEAGNLKITHQADLDLAAAVLTAQRAATEREQ
TA
>Mature_241_residues
GRYWAVIPAAGVGRRMGGGDRPKQYRLLLGRPVIGWALERLLGHPKVAGAVVALAADDPHWDALGLDRQVAGKPVHRVEG
GAERRDSVRAALAYLGGIANPEDRVLVHDAVRPCLSAAELDRLIDEGGAAVDGALLATPVRDTLKRADGDCVGATVSREG
LWQAQTPQLFPLRRLSAALDAALAAGVAVTDEAQAVEWHDGQPRLVTGEAGNLKITHQADLDLAAAVLTAQRAATEREQT
A

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP)

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family

Homologues:

Organism=Escherichia coli, GI1789104, Length=228, Percent_Identity=42.9824561403509, Blast_Score=150, Evalue=6e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPD_HALHL (A1WWZ0)

Other databases:

- EMBL:   CP000544
- RefSeq:   YP_001003004.1
- ProteinModelPortal:   A1WWZ0
- SMR:   A1WWZ0
- STRING:   A1WWZ0
- GeneID:   4710722
- GenomeReviews:   CP000544_GR
- KEGG:   hha:Hhal_1435
- eggNOG:   COG1211
- HOGENOM:   HBG672839
- OMA:   FPQGAIL
- PhylomeDB:   A1WWZ0
- ProtClustDB:   CLSK727477
- BioCyc:   HHAL349124:HHAL_1435-MONOMER
- HAMAP:   MF_00108
- InterPro:   IPR001228
- InterPro:   IPR018294
- TIGRFAMs:   TIGR00453

Pfam domain/function: PF01128 IspD

EC number: =2.7.7.60

Molecular weight: Translated: 25520; Mature: 25389

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRYWAVIPAAGVGRRMGGGDRPKQYRLLLGRPVIGWALERLLGHPKVAGAVVALAADDP
CCCEEEEECCCCCCCCCCCCCCCHHEEHEECCCHHHHHHHHHHCCCCHHCEEEEEECCCC
HWDALGLDRQVAGKPVHRVEGGAERRDSVRAALAYLGGIANPEDRVLVHDAVRPCLSAAE
CCCCCCCCHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
LDRLIDEGGAAVDGALLATPVRDTLKRADGDCVGATVSREGLWQAQTPQLFPLRRLSAAL
HHHHHHCCCCCCCCHHEEHHHHHHHHHCCCCEEECEECCCCCCCCCCCCCCHHHHHHHHH
DAALAAGVAVTDEAQAVEWHDGQPRLVTGEAGNLKITHQADLDLAAAVLTAQRAATEREQ
HHHHHCCEEECCCCCEEEECCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHC
TA
CC
>Mature Secondary Structure 
GRYWAVIPAAGVGRRMGGGDRPKQYRLLLGRPVIGWALERLLGHPKVAGAVVALAADDP
CCEEEEECCCCCCCCCCCCCCCHHEEHEECCCHHHHHHHHHHCCCCHHCEEEEEECCCC
HWDALGLDRQVAGKPVHRVEGGAERRDSVRAALAYLGGIANPEDRVLVHDAVRPCLSAAE
CCCCCCCCHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHH
LDRLIDEGGAAVDGALLATPVRDTLKRADGDCVGATVSREGLWQAQTPQLFPLRRLSAAL
HHHHHHCCCCCCCCHHEEHHHHHHHHHCCCCEEECEECCCCCCCCCCCCCCHHHHHHHHH
DAALAAGVAVTDEAQAVEWHDGQPRLVTGEAGNLKITHQADLDLAAAVLTAQRAATEREQ
HHHHHCCEEECCCCCEEEECCCCCEEEECCCCCEEEEECCCHHHHHHHHHHHHHHHHHHC
TA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA